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The map label for this gene is yjfH [C]

Identifier: 150006258

GI number: 150006258

Start: 4656096

End: 4656902

Strand: Reverse

Name: yjfH [C]

Synonym: BVU_3769

Alternate gene names: 150006258

Gene position: 4656902-4656096 (Counterclockwise)

Preceding gene: 150006259

Following gene: 150006255

Centisome position: 90.19

GC content: 48.33

Gene sequence:

>807_bases
ATGCCTATTATTGAAATATCATCATTGACACATCCCGGAGTGGATGTTTTCTGCATGTTGACGGAAGCTCAGTTACGTCA
GCGTATGGAACCTGGGAAAGGGGTATTTATAGCCGAAAGCCCCAATGTCATTAGTAGGGCTTTGGATGCAGGATATGAAC
CTCTTGCTCTTATGTGTGAGCGTAAGCATATCTCTGGCAGTGCTGCTCACATTATCAAGCGTTGTGGTGATGTGCCCGTT
TATACGGGTGATAGAGAGTTGTTGACTACGTTGACTGGCTATGTTCTGACACGTGGTGTATTATGTGCTATGCGTCGTCC
AGTGTTGCCCTCTATGGAAGAGATCTGCCGGGATACCCGGCGGATTGTAGTCATTGACGGTGTGGTGGACACTACCAATA
TCGGGGCGATATTTCGTTCGGCAGCTGCTCTTGGGATAGATGCTGTTTTGTTGACACGCAATTCTTGCGATCCGTTGAAT
CGCCGTGCGGTAAGGGTGTCTATGGGTTCGGTGTTCTTGGTTCCATGGACATGGTTGAATGGTACGATTGGCGATTTGAA
CAAGTTTGGTTTCCGTACTGCTGCCATGGCGTTGACTGATGATTCTATCTCTATAGATCATCCCATATTGGCGGCTGAAC
CTCGGTTGGCTATTGTGATGGGAACAGAAGGGGATGGGCTTCCTTACAAGACAATTGCTGAAGCTGATTATGTAGTCCGT
ATCCCTATGGCGCATGGAGTGGATTCGCTCAATGTGGCTGCAGCAGCTGCTGTTGCCTTTTGGCAACTCCGGATTACAGA
CCTTTGA

Upstream 100 bases:

>100_bases
AGATTTTTGAGAACCGACAAGATGGAGTGATAAAAGTAGCTGTGACAATGGAGGCTGGAATGTAGATTAAAAGCAATGAA
ATAGTATAATATAAGAGTGT

Downstream 100 bases:

>100_bases
AAAGATTAATATATGATTAAGTTTATTTTATTTTCATAGCTCCCATGGCGTCCGGGCAGTCTGTGGGGGCCTTAGTTGTA
TAGGCATAAAGCGTATCATC

Product: putative rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MPIIEISSLTHPGVDVFCMLTEAQLRQRMEPGKGVFIAESPNVISRALDAGYEPLALMCERKHISGSAAHIIKRCGDVPV
YTGDRELLTTLTGYVLTRGVLCAMRRPVLPSMEEICRDTRRIVVIDGVVDTTNIGAIFRSAAALGIDAVLLTRNSCDPLN
RRAVRVSMGSVFLVPWTWLNGTIGDLNKFGFRTAAMALTDDSISIDHPILAAEPRLAIVMGTEGDGLPYKTIAEADYVVR
IPMAHGVDSLNVAAAAAVAFWQLRITDL

Sequences:

>Translated_268_residues
MPIIEISSLTHPGVDVFCMLTEAQLRQRMEPGKGVFIAESPNVISRALDAGYEPLALMCERKHISGSAAHIIKRCGDVPV
YTGDRELLTTLTGYVLTRGVLCAMRRPVLPSMEEICRDTRRIVVIDGVVDTTNIGAIFRSAAALGIDAVLLTRNSCDPLN
RRAVRVSMGSVFLVPWTWLNGTIGDLNKFGFRTAAMALTDDSISIDHPILAAEPRLAIVMGTEGDGLPYKTIAEADYVVR
IPMAHGVDSLNVAAAAAVAFWQLRITDL
>Mature_267_residues
PIIEISSLTHPGVDVFCMLTEAQLRQRMEPGKGVFIAESPNVISRALDAGYEPLALMCERKHISGSAAHIIKRCGDVPVY
TGDRELLTTLTGYVLTRGVLCAMRRPVLPSMEEICRDTRRIVVIDGVVDTTNIGAIFRSAAALGIDAVLLTRNSCDPLNR
RAVRVSMGSVFLVPWTWLNGTIGDLNKFGFRTAAMALTDDSISIDHPILAAEPRLAIVMGTEGDGLPYKTIAEADYVVRI
PMAHGVDSLNVAAAAAVAFWQLRITDL

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=150, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29061; Mature: 28930

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIIEISSLTHPGVDVFCMLTEAQLRQRMEPGKGVFIAESPNVISRALDAGYEPLALMCE
CCEEEECCCCCCCCEEEEEEEHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHHHEE
RKHISGSAAHIIKRCGDVPVYTGDRELLTTLTGYVLTRGVLCAMRRPVLPSMEEICRDTR
CCCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
RIVVIDGVVDTTNIGAIFRSAAALGIDAVLLTRNSCDPLNRRAVRVSMGSVFLVPWTWLN
EEEEEECEECCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEECCEEEEEEEECC
GTIGDLNKFGFRTAAMALTDDSISIDHPILAAEPRLAIVMGTEGDGLPYKTIAEADYVVR
CCHHHHHHHCHHEEEEEEECCCCCCCCCEEECCCCEEEEECCCCCCCCCCEECCCCEEEE
IPMAHGVDSLNVAAAAAVAFWQLRITDL
EEHHCCCCCHHHHHHHHHHEEEEEEECC
>Mature Secondary Structure 
PIIEISSLTHPGVDVFCMLTEAQLRQRMEPGKGVFIAESPNVISRALDAGYEPLALMCE
CEEEECCCCCCCCEEEEEEEHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHHHEE
RKHISGSAAHIIKRCGDVPVYTGDRELLTTLTGYVLTRGVLCAMRRPVLPSMEEICRDTR
CCCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
RIVVIDGVVDTTNIGAIFRSAAALGIDAVLLTRNSCDPLNRRAVRVSMGSVFLVPWTWLN
EEEEEECEECCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEECCEEEEEEEECC
GTIGDLNKFGFRTAAMALTDDSISIDHPILAAEPRLAIVMGTEGDGLPYKTIAEADYVVR
CCHHHHHHHCHHEEEEEEECCCCCCCCCEEECCCCEEEEECCCCCCCCCCEECCCCEEEE
IPMAHGVDSLNVAAAAAVAFWQLRITDL
EEHHCCCCCHHHHHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]