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The map label for this gene is rsmE [H]

Identifier: 150006102

GI number: 150006102

Start: 4477557

End: 4478258

Strand: Reverse

Name: rsmE [H]

Synonym: BVU_3611

Alternate gene names: 150006102

Gene position: 4478258-4477557 (Counterclockwise)

Preceding gene: 150006103

Following gene: 150006101

Centisome position: 86.73

GC content: 44.02

Gene sequence:

>702_bases
ATGCATGTTTTTTATACCCCTGATATAGCGAGTAACCTTGAGCTTCCGGAAGAGGAAGCAGGACATTGTTTACGGGTTTT
GCGCCTTTCCATAGGCGATGAGATTATGTTGACCGATGGGAAAGGTTGTTTTTACAAAGCTGTAATCAGTGCTGCAAGCG
GTAAACGTTGCCAAGTGAAGGTAACGGAAACGATTCCGCAGGAAAAAGGGTGGAACGGTTGGTTGCATATTGCCATGGCT
CCCACTAAAAATATGGATCGTACTGAATGGTTTACAGAGAAAGCGACTGAAATAGGCTTTGATGAACTGTCTTTCCTGAA
CTGCCGTTATTCCGAACGGAAAGTAATCAAGAAGGAACGTATTGAAAAGATTCTGGTATCGGCAGTGAAACAATCGTTGA
AGGCGACTATGCCGGTGCTGAATGAAATGACGGACTTTAATAAGTTTGTTTCACGGGATTTTAAAGGGCAGAAGTTCATC
GCCCATTGCTATGAAGGGGAAAAACCTTTGTTGAAAGATATATTGCGTTCCGGAGAAGATGCTTTGGTGATGATAGGTCC
GGAAGGAGATTTTAGCGAGGAAGAAGTGGCTAAGGCTATAGCGGCGGGTTTTCAGCCTGTCAGTTTGGGAAAGTCCCGTC
TTCGCACGGAAACAGCTGCATTGGTAGCCTGTCATACGCTGAATTTATTAAACCAAAAATAA

Upstream 100 bases:

>100_bases
TATGAGCAGGCATCCCGTTTGCGTGATGAGATAAAACGACGGGAACAAGAGGAGGAGAATACAATAGCTTGATATATAAA
TTGAAAAAACAAGAAGAACT

Downstream 100 bases:

>100_bases
GAATATGAGAAAAGGTATTTGGGGATTTGCACTCGTAGCGATAGTGCTGCTTATGGCTTCTTGTTCGTCTGAAAGTGAGT
ATGCCAACGCTATTCCTAAG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MHVFYTPDIASNLELPEEEAGHCLRVLRLSIGDEIMLTDGKGCFYKAVISAASGKRCQVKVTETIPQEKGWNGWLHIAMA
PTKNMDRTEWFTEKATEIGFDELSFLNCRYSERKVIKKERIEKILVSAVKQSLKATMPVLNEMTDFNKFVSRDFKGQKFI
AHCYEGEKPLLKDILRSGEDALVMIGPEGDFSEEEVAKAIAAGFQPVSLGKSRLRTETAALVACHTLNLLNQK

Sequences:

>Translated_233_residues
MHVFYTPDIASNLELPEEEAGHCLRVLRLSIGDEIMLTDGKGCFYKAVISAASGKRCQVKVTETIPQEKGWNGWLHIAMA
PTKNMDRTEWFTEKATEIGFDELSFLNCRYSERKVIKKERIEKILVSAVKQSLKATMPVLNEMTDFNKFVSRDFKGQKFI
AHCYEGEKPLLKDILRSGEDALVMIGPEGDFSEEEVAKAIAAGFQPVSLGKSRLRTETAALVACHTLNLLNQK
>Mature_233_residues
MHVFYTPDIASNLELPEEEAGHCLRVLRLSIGDEIMLTDGKGCFYKAVISAASGKRCQVKVTETIPQEKGWNGWLHIAMA
PTKNMDRTEWFTEKATEIGFDELSFLNCRYSERKVIKKERIEKILVSAVKQSLKATMPVLNEMTDFNKFVSRDFKGQKFI
AHCYEGEKPLLKDILRSGEDALVMIGPEGDFSEEEVAKAIAAGFQPVSLGKSRLRTETAALVACHTLNLLNQK

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=228, Percent_Identity=28.0701754385965, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26175; Mature: 26175

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVFYTPDIASNLELPEEEAGHCLRVLRLSIGDEIMLTDGKGCFYKAVISAASGKRCQVK
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCEEEEE
VTETIPQEKGWNGWLHIAMAPTKNMDRTEWFTEKATEIGFDELSFLNCRYSERKVIKKER
ECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHH
IEKILVSAVKQSLKATMPVLNEMTDFNKFVSRDFKGQKFIAHCYEGEKPLLKDILRSGED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCC
ALVMIGPEGDFSEEEVAKAIAAGFQPVSLGKSRLRTETAALVACHTLNLLNQK
EEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHVFYTPDIASNLELPEEEAGHCLRVLRLSIGDEIMLTDGKGCFYKAVISAASGKRCQVK
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCEEEEE
VTETIPQEKGWNGWLHIAMAPTKNMDRTEWFTEKATEIGFDELSFLNCRYSERKVIKKER
ECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHH
IEKILVSAVKQSLKATMPVLNEMTDFNKFVSRDFKGQKFIAHCYEGEKPLLKDILRSGED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCC
ALVMIGPEGDFSEEEVAKAIAAGFQPVSLGKSRLRTETAALVACHTLNLLNQK
EEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]