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The map label for this gene is miaA

Identifier: 150006075

GI number: 150006075

Start: 4446837

End: 4447760

Strand: Reverse

Name: miaA

Synonym: BVU_3584

Alternate gene names: 150006075

Gene position: 4447760-4446837 (Counterclockwise)

Preceding gene: 150006076

Following gene: 150006074

Centisome position: 86.14

GC content: 41.02

Gene sequence:

>924_bases
ATGGAAAAGTACGATTTGATAACGATATTAGGACCTACCGCTTCAGGAAAGACTCCTTTGGCAGCGGCTTTAGCTTACAA
GTTGGATACCGAAATTATCAGTGGAGATAGCCGGCAGGTATATCGCCGGATGGATTTAGGTACGGGAAAGGATTTGGAAG
ACTATGTCGTGAATGGACAGCAAATACCTTACCATCTGATAGACATTGTAGACCCCGGATATAAATATAATGTATTTGAA
TTTCAACGTGATTTTTTGAATGCTTATGATCAAGTGAGATGGAAAGACAAACTTCCTATATTATGCGGAGGTACGGGGAT
GTATCTTGAATCCGTGTTGAAGGGATATAGGCTGCTTCCTGTTCCAGAGAATCCCAAACTTAGGGACTCGTTAGCAGATA
AATCATTAGCGGAACTCACCCGGTTGTTGTCAACTTACAGGAAACTTCATAACTCTACGGACGTAGATACTGTAAAGCGG
GCTATCCGGGCCATTGAAATAGAAGAGTATTACAAGCATCAATCAGCGGAATACCGTGAGTTCCCGCAAATTCATAGTTT
GATCATTGGAGTAGATATAGCTCGTGAGTTACGTCGTGAAAAAATATCCCACCGTTTGAAGCAGCGTTTGGATGAAGGGA
TGGTAAACGAAGTGAAGGCTTTGTTGGATAGTGGTATTTCTTCCGAGGATTTGATTTATTACGGATTGGAATATAAGTAT
TTGACTTTATATGTCTTGGGTAAACTTTCTTTCAATGAAATGTTTCATCAATTGGAAATAGCTATCCATCAATTTGCCAA
ACGGCAGATGACCTGGTTCCGGGGCATGGAGCGGCGAGGGTTCACTATACATTGGCTGGATGCTTGCCTGCCTATGGAAG
ATAAGGTGGAAAAAATCATTAATTTGTTACACACTAAAAACTAA

Upstream 100 bases:

>100_bases
AAAGCCGTCTGAAGTAATTCAGACGGCTTTTTTTTATGTAGTTTTGCGTGCTAAGTTGCACATTTTCGTTATCTTTGTGC
GACTGTTACAAAAACTTAGG

Downstream 100 bases:

>100_bases
ATGTTATGGCTGTAGAACCAAACAGATGGGGGATTATTTATAATCCGAAAGCCGGAAGCCGTAAGACTCAGAAGCGTTGG
AATGAGATACGTAGCTATAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase 2; DMAPP:tRNA dimethylallyltransferase 2; DMATase 2; Isopentenyl-diphosphate:tRNA isopentenyltransferase 2; IPP transferase 2; IPPT 2; IPTase 2

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MEKYDLITILGPTASGKTPLAAALAYKLDTEIISGDSRQVYRRMDLGTGKDLEDYVVNGQQIPYHLIDIVDPGYKYNVFE
FQRDFLNAYDQVRWKDKLPILCGGTGMYLESVLKGYRLLPVPENPKLRDSLADKSLAELTRLLSTYRKLHNSTDVDTVKR
AIRAIEIEEYYKHQSAEYREFPQIHSLIIGVDIARELRREKISHRLKQRLDEGMVNEVKALLDSGISSEDLIYYGLEYKY
LTLYVLGKLSFNEMFHQLEIAIHQFAKRQMTWFRGMERRGFTIHWLDACLPMEDKVEKIINLLHTKN

Sequences:

>Translated_307_residues
MEKYDLITILGPTASGKTPLAAALAYKLDTEIISGDSRQVYRRMDLGTGKDLEDYVVNGQQIPYHLIDIVDPGYKYNVFE
FQRDFLNAYDQVRWKDKLPILCGGTGMYLESVLKGYRLLPVPENPKLRDSLADKSLAELTRLLSTYRKLHNSTDVDTVKR
AIRAIEIEEYYKHQSAEYREFPQIHSLIIGVDIARELRREKISHRLKQRLDEGMVNEVKALLDSGISSEDLIYYGLEYKY
LTLYVLGKLSFNEMFHQLEIAIHQFAKRQMTWFRGMERRGFTIHWLDACLPMEDKVEKIINLLHTKN
>Mature_307_residues
MEKYDLITILGPTASGKTPLAAALAYKLDTEIISGDSRQVYRRMDLGTGKDLEDYVVNGQQIPYHLIDIVDPGYKYNVFE
FQRDFLNAYDQVRWKDKLPILCGGTGMYLESVLKGYRLLPVPENPKLRDSLADKSLAELTRLLSTYRKLHNSTDVDTVKR
AIRAIEIEEYYKHQSAEYREFPQIHSLIIGVDIARELRREKISHRLKQRLDEGMVNEVKALLDSGISSEDLIYYGLEYKY
LTLYVLGKLSFNEMFHQLEIAIHQFAKRQMTWFRGMERRGFTIHWLDACLPMEDKVEKIINLLHTKN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=112, Percent_Identity=38.3928571428571, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1790613, Length=313, Percent_Identity=31.629392971246, Blast_Score=135, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25144712, Length=110, Percent_Identity=35.4545454545455, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324848, Length=295, Percent_Identity=24.0677966101695, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24649830, Length=113, Percent_Identity=37.1681415929204, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA2_BACV8 (A6L683)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300819.1
- ProteinModelPortal:   A6L683
- SMR:   A6L683
- STRING:   A6L683
- GeneID:   5304544
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_3584
- NMPDR:   fig|435590.6.peg.3332
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   IYYGLEY
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35775; Mature: 35775

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYDLITILGPTASGKTPLAAALAYKLDTEIISGDSRQVYRRMDLGTGKDLEDYVVNGQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHCCCC
QIPYHLIDIVDPGYKYNVFEFQRDFLNAYDQVRWKDKLPILCGGTGMYLESVLKGYRLLP
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHCCCEEEC
VPENPKLRDSLADKSLAELTRLLSTYRKLHNSTDVDTVKRAIRAIEIEEYYKHQSAEYRE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
FPQIHSLIIGVDIARELRREKISHRLKQRLDEGMVNEVKALLDSGISSEDLIYYGLEYKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHE
LTLYVLGKLSFNEMFHQLEIAIHQFAKRQMTWFRGMERRGFTIHWLDACLPMEDKVEKII
EEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCHHHHHHHH
NLLHTKN
HHHHCCC
>Mature Secondary Structure
MEKYDLITILGPTASGKTPLAAALAYKLDTEIISGDSRQVYRRMDLGTGKDLEDYVVNGQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHCCCC
QIPYHLIDIVDPGYKYNVFEFQRDFLNAYDQVRWKDKLPILCGGTGMYLESVLKGYRLLP
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHCCCEEEC
VPENPKLRDSLADKSLAELTRLLSTYRKLHNSTDVDTVKRAIRAIEIEEYYKHQSAEYRE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
FPQIHSLIIGVDIARELRREKISHRLKQRLDEGMVNEVKALLDSGISSEDLIYYGLEYKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHE
LTLYVLGKLSFNEMFHQLEIAIHQFAKRQMTWFRGMERRGFTIHWLDACLPMEDKVEKII
EEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCHHHHHHHH
NLLHTKN
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA