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The map label for this gene is potB [H]

Identifier: 150006051

GI number: 150006051

Start: 4408773

End: 4409570

Strand: Reverse

Name: potB [H]

Synonym: BVU_3555

Alternate gene names: 150006051

Gene position: 4409570-4408773 (Counterclockwise)

Preceding gene: 150006052

Following gene: 150006050

Centisome position: 85.4

GC content: 39.22

Gene sequence:

>798_bases
GTGCTCAGAACGATACGATTTTTCTTGTCTTCACGTAAAAGTTGGACTATTCCTTACGTGATATTTTCGGCGGTATTTGT
TATTCTTCCGCTACTTTTAATAGTGGTTTATGCATTTATGGATGATGCGGGGCATTTCACATTGGGAAATTTTGCCAAAT
TTTTCGCTCATCCCGAGGCAATCAACACATTTGTTTATTCTATTGGCGTGGCCATTATCACAACAGTGGTATGTATCTTA
TTGGGATATCCTGCTGCCTATATTCTGACACAGACGCGGATGAAATATGCATCGACAGTGGTGGTATTGTTTATTTTGCC
CATGTGGGTGAATATTTTAATACGTACACTGGCTACGGTGGCCTTGTTCGATTTTATGAACGTATCTTTAGGAGAGGGGG
CTTTGATATTCGGTATGGTATATAATTTTTTGCCTTTTATGATTTATCCTATTTACAACACTTTGCAGAAATTGGATAAA
AGTTATGTGGAAGCGGCGCAAGATTTGGGCGCCAATCCCATGCAAGTGTTTTTCAAGGCTGTGTTGCCCCTGTCTATGCC
GGGTATTGTGAGTGGTATATTAATGGTATTTATGCCTACTATCTCCACATTTGCTATTGCCGAATTATTGACAATGAATA
ATATTAAATTATTTGGCACTACTATCCAGGAAAATATTAATAATAGTATGTGGAATTATGGGGCGGCCTTGTCGTTGATC
ATGTTGTTCCTTATTGGTGCGACAACCTTGCTCGCTTCAGATGATAAGGATCATAACGAAGGAGGTGGACTATGGTAA

Upstream 100 bases:

>100_bases
TACCAATGATGTATGGGACAATGGTGATCATGTAGGTATCAGCATCTTTCCTGAAAGTATAAAAATAACTCAGATTGTAG
AATCATAATAAAGAAAAGAG

Downstream 100 bases:

>100_bases
AGAAAATTTTGTGTCAGGGGTATTTGTGGCTGCTTCTGCTATTGCTTTATGCGCCTATTTTTATCATTATGATCTATTCG
TTTACCGAAGCAAAGGTGCT

Product: putrescine transport system permease protein PotH

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MLRTIRFFLSSRKSWTIPYVIFSAVFVILPLLLIVVYAFMDDAGHFTLGNFAKFFAHPEAINTFVYSIGVAIITTVVCIL
LGYPAAYILTQTRMKYASTVVVLFILPMWVNILIRTLATVALFDFMNVSLGEGALIFGMVYNFLPFMIYPIYNTLQKLDK
SYVEAAQDLGANPMQVFFKAVLPLSMPGIVSGILMVFMPTISTFAIAELLTMNNIKLFGTTIQENINNSMWNYGAALSLI
MLFLIGATTLLASDDKDHNEGGGLW

Sequences:

>Translated_265_residues
MLRTIRFFLSSRKSWTIPYVIFSAVFVILPLLLIVVYAFMDDAGHFTLGNFAKFFAHPEAINTFVYSIGVAIITTVVCIL
LGYPAAYILTQTRMKYASTVVVLFILPMWVNILIRTLATVALFDFMNVSLGEGALIFGMVYNFLPFMIYPIYNTLQKLDK
SYVEAAQDLGANPMQVFFKAVLPLSMPGIVSGILMVFMPTISTFAIAELLTMNNIKLFGTTIQENINNSMWNYGAALSLI
MLFLIGATTLLASDDKDHNEGGGLW
>Mature_265_residues
MLRTIRFFLSSRKSWTIPYVIFSAVFVILPLLLIVVYAFMDDAGHFTLGNFAKFFAHPEAINTFVYSIGVAIITTVVCIL
LGYPAAYILTQTRMKYASTVVVLFILPMWVNILIRTLATVALFDFMNVSLGEGALIFGMVYNFLPFMIYPIYNTLQKLDK
SYVEAAQDLGANPMQVFFKAVLPLSMPGIVSGILMVFMPTISTFAIAELLTMNNIKLFGTTIQENINNSMWNYGAALSLI
MLFLIGATTLLASDDKDHNEGGGLW

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=246, Percent_Identity=34.5528455284553, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1787080, Length=218, Percent_Identity=34.8623853211009, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1787713, Length=200, Percent_Identity=26.5, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787368, Length=200, Percent_Identity=24.5, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29515; Mature: 29515

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]