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The map label for this gene is ispE

Identifier: 150005968

GI number: 150005968

Start: 4317092

End: 4317919

Strand: Reverse

Name: ispE

Synonym: BVU_3466

Alternate gene names: 150005968

Gene position: 4317919-4317092 (Counterclockwise)

Preceding gene: 150005973

Following gene: 150005962

Centisome position: 83.63

GC content: 42.51

Gene sequence:

>828_bases
ATGATTACATTTCCTAATGCAAAGATTAATTTGGGACTTAATATTGTAGAAAAGCGCCCGGATGGATACCACAATCTGGA
AACCATATTTTATCCGATAAACCTGCAAGACGCTCTGGAAGTGACGCGGCGGGAAAACAATGACAAAGAATACACTTTAC
ACATAAGCGGATCTCCTTTGGAGGGTGAACCCGAAGATAATCTGGTAGTACAAGCCTACAAGTTATTAAAAAAAGACTAT
CCCGGACTGTTGCCGGTAGATATACACATGTACAAGCATATACCGGCGGGTGCCGGACTGGGTGGCGGATCATCAGATGC
CGCTTGTATGATAAAGCTTCTGAATGATAAATTTTCATTGGGACTCAGTACGGAACGGATGGAGGAATATGCGGTAAAAC
TAGGGGCAGACTGTGCTTTTTTCATTCGGAACAAACCTGTTTTCGCTACCGGAATAGGGAATCTGTTCGAGCCTGTGGAA
TTGTCATTAAAGGGATACCACATAATTTTGATTAAACCTGATATCTTTGTGTCCACCCGGGATGCGTTTGCCGAAATAAA
GCCTGTGCGTCCGGCTGTTTCTTTGAAAGAAATTGTAAAGCAACCTATGGAAACATGGAAAAACAGTATGAAGAATGATT
TTGAAGATAGTGTCTTCAAAAAGTTCCCTGAGATTGCTGCTATAAAAGATGAATTGTATGATTTGGGGGCAGTGTATGCC
GCCATGAGTGGCTCGGGGTCTTCCGTCTATGGTATTTTCAAAGCACCGATAGAGAATGTGGAAGATAAATTCTGTGGATG
CTTTTGCAGACAGAGAGCGTTGGAATAG

Upstream 100 bases:

>100_bases
ATATTTGGCGCTCTGTCCGGATAAAAGATGGCGTGCATCTTTGTAGAACGGGCAACAATCTAACGAAACACACTAAAAAT
AACTATTTAAAGAACAGAAT

Downstream 100 bases:

>100_bases
TGTGGCAGATCAGTGGTTAGTGATTAGTGGACTGCGCTGTCATGCCGCAGGTATTAACCACTAACCACTATAGTCTATCT
TTTACATAGTGCCTTGAAAT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MITFPNAKINLGLNIVEKRPDGYHNLETIFYPINLQDALEVTRRENNDKEYTLHISGSPLEGEPEDNLVVQAYKLLKKDY
PGLLPVDIHMYKHIPAGAGLGGGSSDAACMIKLLNDKFSLGLSTERMEEYAVKLGADCAFFIRNKPVFATGIGNLFEPVE
LSLKGYHIILIKPDIFVSTRDAFAEIKPVRPAVSLKEIVKQPMETWKNSMKNDFEDSVFKKFPEIAAIKDELYDLGAVYA
AMSGSGSSVYGIFKAPIENVEDKFCGCFCRQRALE

Sequences:

>Translated_275_residues
MITFPNAKINLGLNIVEKRPDGYHNLETIFYPINLQDALEVTRRENNDKEYTLHISGSPLEGEPEDNLVVQAYKLLKKDY
PGLLPVDIHMYKHIPAGAGLGGGSSDAACMIKLLNDKFSLGLSTERMEEYAVKLGADCAFFIRNKPVFATGIGNLFEPVE
LSLKGYHIILIKPDIFVSTRDAFAEIKPVRPAVSLKEIVKQPMETWKNSMKNDFEDSVFKKFPEIAAIKDELYDLGAVYA
AMSGSGSSVYGIFKAPIENVEDKFCGCFCRQRALE
>Mature_275_residues
MITFPNAKINLGLNIVEKRPDGYHNLETIFYPINLQDALEVTRRENNDKEYTLHISGSPLEGEPEDNLVVQAYKLLKKDY
PGLLPVDIHMYKHIPAGAGLGGGSSDAACMIKLLNDKFSLGLSTERMEEYAVKLGADCAFFIRNKPVFATGIGNLFEPVE
LSLKGYHIILIKPDIFVSTRDAFAEIKPVRPAVSLKEIVKQPMETWKNSMKNDFEDSVFKKFPEIAAIKDELYDLGAVYA
AMSGSGSSVYGIFKAPIENVEDKFCGCFCRQRALE

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=255, Percent_Identity=34.1176470588235, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_BACV8 (A6L5X6)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300712.1
- ProteinModelPortal:   A6L5X6
- SMR:   A6L5X6
- STRING:   A6L5X6
- GeneID:   5304426
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_3466
- NMPDR:   fig|435590.6.peg.3232
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- ProtClustDB:   PRK14609
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30680; Mature: 30680

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITFPNAKINLGLNIVEKRPDGYHNLETIFYPINLQDALEVTRRENNDKEYTLHISGSPL
CCCCCCCEEEECEEEEECCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEEEECCCC
EGEPEDNLVVQAYKLLKKDYPGLLPVDIHMYKHIPAGAGLGGGSSDAACMIKLLNDKFSL
CCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHCC
GLSTERMEEYAVKLGADCAFFIRNKPVFATGIGNLFEPVELSLKGYHIILIKPDIFVSTR
CCCHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCEEEECCEEEEEECCCEEEECC
DAFAEIKPVRPAVSLKEIVKQPMETWKNSMKNDFEDSVFKKFPEIAAIKDELYDLGAVYA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
AMSGSGSSVYGIFKAPIENVEDKFCGCFCRQRALE
HHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITFPNAKINLGLNIVEKRPDGYHNLETIFYPINLQDALEVTRRENNDKEYTLHISGSPL
CCCCCCCEEEECEEEEECCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEEEECCCC
EGEPEDNLVVQAYKLLKKDYPGLLPVDIHMYKHIPAGAGLGGGSSDAACMIKLLNDKFSL
CCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHCC
GLSTERMEEYAVKLGADCAFFIRNKPVFATGIGNLFEPVELSLKGYHIILIKPDIFVSTR
CCCHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCEEEECCEEEEEECCCEEEECC
DAFAEIKPVRPAVSLKEIVKQPMETWKNSMKNDFEDSVFKKFPEIAAIKDELYDLGAVYA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
AMSGSGSSVYGIFKAPIENVEDKFCGCFCRQRALE
HHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA