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The map label for this gene is proS

Identifier: 150005824

GI number: 150005824

Start: 4154674

End: 4156164

Strand: Reverse

Name: proS

Synonym: BVU_3320

Alternate gene names: 150005824

Gene position: 4156164-4154674 (Counterclockwise)

Preceding gene: 150005825

Following gene: 150005823

Centisome position: 80.5

GC content: 44.27

Gene sequence:

>1491_bases
ATGGCAGTAGAATTGAAAGAACTTACCAAAAGAAGCGAGAATTATTCTCAGTGGTATAATGACTTGGTGGTGAAGGCCGA
TTTGGCTGAACAATCACCTGTGCGTGGATGTATGGTTATCAAGCCTTACGGATACGCCATTTGGGAGAAAATGCAGCGTC
AGTTGGACGATATGTTTAAGGAAACCGGCCACGTGAACGCTTATTTCCCGTTGCTTATCCCGAAATCTTACCTTAGCCGT
GAAGCTGAGCACGTAGAAGGATTTGCCAAAGAGTGTGCTGTAGTTACTCACTACCGTCTGAAGAATGCAGAGGACGGTTC
GGGTGTTATCGTAGATCCGGCTGCCAAACTGGAAGAAGAATTGATTATCCGCCCTACATCTGAGACAATCATTTGGAGTA
CATATAAGAACTGGATTAATTCCTATCGTGACTTGCCTATCTTGTGTAACCAATGGGCAAATGTTATGCGGTGGGAGATG
CGTACCCGTTTGTTCCTGCGTACTGCGGAGTTCTTGTGGCAGGAAGGACATACGGCTCATGCGACCCGCGAGGAAGCAGA
AGCGGAAGCACAGAAAATGTTGCATGTGTATGGTGACTTCGCTGAGAAGTATATGGCTGTTCCGGTAATCAAAGGGGTAA
AATCGGCTAATGAACGTTTCGCCGGTGCATTGGATACTTACACCATCGAAGGCTTGATGCAGGATGGAAAGGCATTGCAA
TGTGGTACTTCTCACTTCTTGGGACAGAATTTCGCAAAAGCGTTCAATGTACAGTTTGTTGATAAGAATAATAAGCTGGA
TTATGTTTGGGCTACTTCCTGGGGAGTATCTACCCGCCTGATGGGTGCTTTGATAATGACTCACTCTGATGATAACGGAT
TGGTGTTGCCACCGCATTTGGCTCCTATTCAGGTAGTTATCGTTCCTATTTATAAGAATGATGAAATGCTGAAGAAGATT
GATGCAAAAGTAGAAGGCATTGTCAATAAGTTGAAAGCAATGGGTATCTCTGTGAAATATGATAATGCAGACAATAAGCG
TCCGGGATTCAAGTTTGCAGATTACGAACTGAAAGGTGTGCCTGTTCGCTTGGTAATGGGTGGCCGTGATCTTGAAAACA
ATACAATGGAAGTGATGCGTCGTGATACATTGGAGAAAGAAACTCGTTCTTGCGACGGAATCGAAGAATATGTACAACAA
TTGTTGGAGGATATCCAGAACAATATTTATCAGAAGGCATTGAACTATCGTAACGAACATATCGTGAAAGTGGATTCATA
CGATGATTTCAAGGAACAAATAGAGAAAGGCGGCTTTATTCTGGCTCATTGGGATGGAACCCCGGAAACGGAAGACCGGA
TTAAAGAGGAAACCAAGGCAACAATTCGTTGTCTTCCTTTTGATGCTGATGAAGAGAGCCTGACTCCGGGCAAGTGTATG
GTAACCGGAAAACCATCTGCTCGCCGTGTTCTCTTTGCGAGAGCATATTAA

Upstream 100 bases:

>100_bases
TTTTGATCCTATATTTTTGCTAAATCATTGGTACTTTCCCATGCTTTTTTACTAATTTTGCAAACTCTTAAAAAGGAAAA
ACAAAAATAACGATATAGCA

Downstream 100 bases:

>100_bases
ACTTCGACACATTTATTTTATTTTAATACATTATGTGGAGTCGGCAGCAGTGAATGCTCCCGACTCTTTTTGTTTTTATG
TGATGATTAATATAGAAAAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MAVELKELTKRSENYSQWYNDLVVKADLAEQSPVRGCMVIKPYGYAIWEKMQRQLDDMFKETGHVNAYFPLLIPKSYLSR
EAEHVEGFAKECAVVTHYRLKNAEDGSGVIVDPAAKLEEELIIRPTSETIIWSTYKNWINSYRDLPILCNQWANVMRWEM
RTRLFLRTAEFLWQEGHTAHATREEAEAEAQKMLHVYGDFAEKYMAVPVIKGVKSANERFAGALDTYTIEGLMQDGKALQ
CGTSHFLGQNFAKAFNVQFVDKNNKLDYVWATSWGVSTRLMGALIMTHSDDNGLVLPPHLAPIQVVIVPIYKNDEMLKKI
DAKVEGIVNKLKAMGISVKYDNADNKRPGFKFADYELKGVPVRLVMGGRDLENNTMEVMRRDTLEKETRSCDGIEEYVQQ
LLEDIQNNIYQKALNYRNEHIVKVDSYDDFKEQIEKGGFILAHWDGTPETEDRIKEETKATIRCLPFDADEESLTPGKCM
VTGKPSARRVLFARAY

Sequences:

>Translated_496_residues
MAVELKELTKRSENYSQWYNDLVVKADLAEQSPVRGCMVIKPYGYAIWEKMQRQLDDMFKETGHVNAYFPLLIPKSYLSR
EAEHVEGFAKECAVVTHYRLKNAEDGSGVIVDPAAKLEEELIIRPTSETIIWSTYKNWINSYRDLPILCNQWANVMRWEM
RTRLFLRTAEFLWQEGHTAHATREEAEAEAQKMLHVYGDFAEKYMAVPVIKGVKSANERFAGALDTYTIEGLMQDGKALQ
CGTSHFLGQNFAKAFNVQFVDKNNKLDYVWATSWGVSTRLMGALIMTHSDDNGLVLPPHLAPIQVVIVPIYKNDEMLKKI
DAKVEGIVNKLKAMGISVKYDNADNKRPGFKFADYELKGVPVRLVMGGRDLENNTMEVMRRDTLEKETRSCDGIEEYVQQ
LLEDIQNNIYQKALNYRNEHIVKVDSYDDFKEQIEKGGFILAHWDGTPETEDRIKEETKATIRCLPFDADEESLTPGKCM
VTGKPSARRVLFARAY
>Mature_495_residues
AVELKELTKRSENYSQWYNDLVVKADLAEQSPVRGCMVIKPYGYAIWEKMQRQLDDMFKETGHVNAYFPLLIPKSYLSRE
AEHVEGFAKECAVVTHYRLKNAEDGSGVIVDPAAKLEEELIIRPTSETIIWSTYKNWINSYRDLPILCNQWANVMRWEMR
TRLFLRTAEFLWQEGHTAHATREEAEAEAQKMLHVYGDFAEKYMAVPVIKGVKSANERFAGALDTYTIEGLMQDGKALQC
GTSHFLGQNFAKAFNVQFVDKNNKLDYVWATSWGVSTRLMGALIMTHSDDNGLVLPPHLAPIQVVIVPIYKNDEMLKKID
AKVEGIVNKLKAMGISVKYDNADNKRPGFKFADYELKGVPVRLVMGGRDLENNTMEVMRRDTLEKETRSCDGIEEYVQQL
LEDIQNNIYQKALNYRNEHIVKVDSYDDFKEQIEKGGFILAHWDGTPETEDRIKEETKATIRCLPFDADEESLTPGKCMV
TGKPSARRVLFARAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=509, Percent_Identity=38.8998035363458, Blast_Score=356, Evalue=3e-98,
Organism=Escherichia coli, GI1786392, Length=175, Percent_Identity=31.4285714285714, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71991342, Length=507, Percent_Identity=38.2642998027613, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71991346, Length=370, Percent_Identity=42.4324324324324, Blast_Score=317, Evalue=8e-87,
Organism=Saccharomyces cerevisiae, GI6321808, Length=514, Percent_Identity=37.9377431906615, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24649468, Length=510, Percent_Identity=37.4509803921569, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI24649466, Length=510, Percent_Identity=37.4509803921569, Blast_Score=342, Evalue=5e-94,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_BACV8 (A6L5I2)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300568.1
- ProteinModelPortal:   A6L5I2
- SMR:   A6L5I2
- STRING:   A6L5I2
- GeneID:   5304281
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_3320
- NMPDR:   fig|435590.6.peg.3094
- eggNOG:   COG0442
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 56831; Mature: 56699

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVELKELTKRSENYSQWYNDLVVKADLAEQSPVRGCMVIKPYGYAIWEKMQRQLDDMFK
CCCCHHHHHHCCCHHHHHHHHHEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
ETGHVNAYFPLLIPKSYLSREAEHVEGFAKECAVVTHYRLKNAEDGSGVIVDPAAKLEEE
HCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCHHHCCCC
LIIRPTSETIIWSTYKNWINSYRDLPILCNQWANVMRWEMRTRLFLRTAEFLWQEGHTAH
EEEECCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATREEAEAEAQKMLHVYGDFAEKYMAVPVIKGVKSANERFAGALDTYTIEGLMQDGKALQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEE
CGTSHFLGQNFAKAFNVQFVDKNNKLDYVWATSWGVSTRLMGALIMTHSDDNGLVLPPHL
CCCHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCHHHHHHHEEEEEECCCCCEEECCCC
APIQVVIVPIYKNDEMLKKIDAKVEGIVNKLKAMGISVKYDNADNKRPGFKFADYELKGV
CCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCEEECEEECCC
PVRLVMGGRDLENNTMEVMRRDTLEKETRSCDGIEEYVQQLLEDIQNNIYQKALNYRNEH
CEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
IVKVDSYDDFKEQIEKGGFILAHWDGTPETEDRIKEETKATIRCLPFDADEESLTPGKCM
EEEECCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCEEE
VTGKPSARRVLFARAY
EECCCCCCEEEEEECC
>Mature Secondary Structure 
AVELKELTKRSENYSQWYNDLVVKADLAEQSPVRGCMVIKPYGYAIWEKMQRQLDDMFK
CCCHHHHHHCCCHHHHHHHHHEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
ETGHVNAYFPLLIPKSYLSREAEHVEGFAKECAVVTHYRLKNAEDGSGVIVDPAAKLEEE
HCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCHHHCCCC
LIIRPTSETIIWSTYKNWINSYRDLPILCNQWANVMRWEMRTRLFLRTAEFLWQEGHTAH
EEEECCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATREEAEAEAQKMLHVYGDFAEKYMAVPVIKGVKSANERFAGALDTYTIEGLMQDGKALQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEE
CGTSHFLGQNFAKAFNVQFVDKNNKLDYVWATSWGVSTRLMGALIMTHSDDNGLVLPPHL
CCCHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCHHHHHHHEEEEEECCCCCEEECCCC
APIQVVIVPIYKNDEMLKKIDAKVEGIVNKLKAMGISVKYDNADNKRPGFKFADYELKGV
CCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCEEECEEECCC
PVRLVMGGRDLENNTMEVMRRDTLEKETRSCDGIEEYVQQLLEDIQNNIYQKALNYRNEH
CEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
IVKVDSYDDFKEQIEKGGFILAHWDGTPETEDRIKEETKATIRCLPFDADEESLTPGKCM
EEEECCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCEEE
VTGKPSARRVLFARAY
EECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA