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The map label for this gene is ligA

Identifier: 150005810

GI number: 150005810

Start: 4141977

End: 4143974

Strand: Reverse

Name: ligA

Synonym: BVU_3306

Alternate gene names: 150005810

Gene position: 4143974-4141977 (Counterclockwise)

Preceding gene: 150005813

Following gene: 150005809

Centisome position: 80.26

GC content: 44.14

Gene sequence:

>1998_bases
ATGACAGAAATAGAAAGAATAGACCAATTGCGCGAGGAGCTTCATCGACATAACTATAATTATTATGTATTGAATGCGCC
TGAGATTACAGACCAGGAGTTTGACAAGCTGATGCGTGAACTTCAGGATCTGGAAGAAAAACATCCGGAACATAGAGATG
AGAACTCGCCCAGTATGCGTGTGGGAAGTGATATAAACAAAAATTTCACGCAGGTGGTACATAAATATCCTATGCTTTCA
TTGGCTAACACCTATTCGGAAACGGAAGTAACCGAGTTTTATGACAGGGTAAAGAAGTCTCTGAACGAGGATTTTGAAAT
TTGTTGTGAAATGAAGTATGATGGAACTTCCATCTCTCTGACTTATGAGAACGGAAAACTGGTGCGCGCCGTGACGCGTG
GCGATGGTGTGCAGGGAGATGATGTGACAGGCAATGTAAAGACCATCCGCAGTATCCCTTTGGTGCTGCATGGAAAGGGG
TATCCTGAAACATTTGAAATTCGTGGGGAGATTTTGATGCCTTGGGTGGTGTTTGAGGAATTGAACAAAGAACGGGAAGC
CCGCGAAGAACCGTTGTTCGCCAATCCCCGCAACGCGGCTTCGGGAACATTGAAGTTGCAGAATTCTGCTATTGTGGCAT
CTCGCAAGCTGGATGCCTACCTTTATTATTTACTTGGAGATAATTTGCCGTGCGACGGTCATTATGAAAATCTGAAAGAA
GCTGAGAAATGGGGATTTAAAATTTCCGATCTCACTCGCAAATGCAAAACATTGCAGGAAGTTTTTGATTTTATCAAATA
CTGGGATGTGGAGCGTAAGAATCTGCCTGTAGCTACGGATGGAATAGTATTGAAAGTGAATTCTTTGCGACAGCAGCGTA
ATTTGGGTTTTACGGCAAAATCACCCCGATGGGCCATTGCATACAAGTTTCAAGCCGAACAGGCACTTACCCGCTTGAAC
AAGGTGACCTATCAAGTGGGGAGAACAGGTGCTGTGACTCCGGTGGCCAATCTGGATCCTATACAGTTGTCCGGAACCGT
GGTAAAGCGTGCTTCGCTCCATAATGCCGACATCATAGAAGGGTTGGATTTGCATATTGGTGATATGGTATATGTAGAAA
AAGGCGGTGAGATTATTCCAAAGATTGTAGGTGTGGACAAAGACGCCCGTATTATGATAGGTGACAAAGTAAAGTTTATT
ACTCATTGTCCCGAGTGTGGCAGTAAATTGGTACGCTATGAAGGTGAAGCTGCTTATTATTGTACCAATGATGCCGCTTG
CCCCCCACAGATCAAGGGAAAAATAGAACATTTTATCAGCCGTAGAGCTATGAATATTGATGGTCTCGGTCCGGAAACGG
TAGATCAGTTTTACCAGGAAGGATTGATACGTGATGTTGCCGATCTGTATACTTTAAAAACATCGGATATCATCAACCTA
GAACGTATGGGAGAAAAATCTGCCGAGAATATAATAAAAGGTATAGAGCAGTCCAAAGAAGTGCCGTTCGAAAGGGTACT
CTTTGCATTGGGCATCCGTTTTGTGGGTGAAACTGTGGCGAAGAAGGTGGCAAAATCCTTTAAGTCGATGGATGCTTTGG
CAGACGCGAGTTTGGATAATTTGATTCATGTCGATGAAATCGGAGAAAAAATAGCGCAGAGTATCTTGTTGTATTTTGCT
AATCCGAAGAATCGTGACATTATAGAGCGTCTTCGTGTGGCGGGTGTGAGACTGGAAGCCGATGAGGAGGATACTTCGGA
ACACACTGACAAACTGGCAGGGAAATCTATCGTGATCAGTGGGGTTTTCGCCCATCATTCGCGTGACGAATATAAGGAGT
TAATTGAAAAGCATGGCGGTAAGAATGTGGGAAGTATTTCGTCCAAGACCAGCTTTATTCTGGCAGGTGACAATATGGGG
CCCAGTAAACTGGAGAAAGCGCAGAAACTGGGTGTGACCATTGTGAGCGAAGAGGAGTTTCTGCAAATGATAGAGTGA

Upstream 100 bases:

>100_bases
ATATTCGGTTATTTTTTGCAAAAGTAGCAAATTCCTCTTTGTCGGAAGGTGTTTTTCATTCAAAAAATAGCTATTTTTGC
GGGAAAACTACCTTTTATAT

Downstream 100 bases:

>100_bases
CTTTTTAGCGAGTATAAAGTAAAAATAGTGTGATTTTTATCGCTAAAATAGAAAATATTCGTACTTTTGTAGGCTATTAA
ATAAGAATTTATAATTCATT

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MTEIERIDQLREELHRHNYNYYVLNAPEITDQEFDKLMRELQDLEEKHPEHRDENSPSMRVGSDINKNFTQVVHKYPMLS
LANTYSETEVTEFYDRVKKSLNEDFEICCEMKYDGTSISLTYENGKLVRAVTRGDGVQGDDVTGNVKTIRSIPLVLHGKG
YPETFEIRGEILMPWVVFEELNKEREAREEPLFANPRNAASGTLKLQNSAIVASRKLDAYLYYLLGDNLPCDGHYENLKE
AEKWGFKISDLTRKCKTLQEVFDFIKYWDVERKNLPVATDGIVLKVNSLRQQRNLGFTAKSPRWAIAYKFQAEQALTRLN
KVTYQVGRTGAVTPVANLDPIQLSGTVVKRASLHNADIIEGLDLHIGDMVYVEKGGEIIPKIVGVDKDARIMIGDKVKFI
THCPECGSKLVRYEGEAAYYCTNDAACPPQIKGKIEHFISRRAMNIDGLGPETVDQFYQEGLIRDVADLYTLKTSDIINL
ERMGEKSAENIIKGIEQSKEVPFERVLFALGIRFVGETVAKKVAKSFKSMDALADASLDNLIHVDEIGEKIAQSILLYFA
NPKNRDIIERLRVAGVRLEADEEDTSEHTDKLAGKSIVISGVFAHHSRDEYKELIEKHGGKNVGSISSKTSFILAGDNMG
PSKLEKAQKLGVTIVSEEEFLQMIE

Sequences:

>Translated_665_residues
MTEIERIDQLREELHRHNYNYYVLNAPEITDQEFDKLMRELQDLEEKHPEHRDENSPSMRVGSDINKNFTQVVHKYPMLS
LANTYSETEVTEFYDRVKKSLNEDFEICCEMKYDGTSISLTYENGKLVRAVTRGDGVQGDDVTGNVKTIRSIPLVLHGKG
YPETFEIRGEILMPWVVFEELNKEREAREEPLFANPRNAASGTLKLQNSAIVASRKLDAYLYYLLGDNLPCDGHYENLKE
AEKWGFKISDLTRKCKTLQEVFDFIKYWDVERKNLPVATDGIVLKVNSLRQQRNLGFTAKSPRWAIAYKFQAEQALTRLN
KVTYQVGRTGAVTPVANLDPIQLSGTVVKRASLHNADIIEGLDLHIGDMVYVEKGGEIIPKIVGVDKDARIMIGDKVKFI
THCPECGSKLVRYEGEAAYYCTNDAACPPQIKGKIEHFISRRAMNIDGLGPETVDQFYQEGLIRDVADLYTLKTSDIINL
ERMGEKSAENIIKGIEQSKEVPFERVLFALGIRFVGETVAKKVAKSFKSMDALADASLDNLIHVDEIGEKIAQSILLYFA
NPKNRDIIERLRVAGVRLEADEEDTSEHTDKLAGKSIVISGVFAHHSRDEYKELIEKHGGKNVGSISSKTSFILAGDNMG
PSKLEKAQKLGVTIVSEEEFLQMIE
>Mature_664_residues
TEIERIDQLREELHRHNYNYYVLNAPEITDQEFDKLMRELQDLEEKHPEHRDENSPSMRVGSDINKNFTQVVHKYPMLSL
ANTYSETEVTEFYDRVKKSLNEDFEICCEMKYDGTSISLTYENGKLVRAVTRGDGVQGDDVTGNVKTIRSIPLVLHGKGY
PETFEIRGEILMPWVVFEELNKEREAREEPLFANPRNAASGTLKLQNSAIVASRKLDAYLYYLLGDNLPCDGHYENLKEA
EKWGFKISDLTRKCKTLQEVFDFIKYWDVERKNLPVATDGIVLKVNSLRQQRNLGFTAKSPRWAIAYKFQAEQALTRLNK
VTYQVGRTGAVTPVANLDPIQLSGTVVKRASLHNADIIEGLDLHIGDMVYVEKGGEIIPKIVGVDKDARIMIGDKVKFIT
HCPECGSKLVRYEGEAAYYCTNDAACPPQIKGKIEHFISRRAMNIDGLGPETVDQFYQEGLIRDVADLYTLKTSDIINLE
RMGEKSAENIIKGIEQSKEVPFERVLFALGIRFVGETVAKKVAKSFKSMDALADASLDNLIHVDEIGEKIAQSILLYFAN
PKNRDIIERLRVAGVRLEADEEDTSEHTDKLAGKSIVISGVFAHHSRDEYKELIEKHGGKNVGSISSKTSFILAGDNMGP
SKLEKAQKLGVTIVSEEEFLQMIE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=666, Percent_Identity=43.5435435435435, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI87082305, Length=513, Percent_Identity=22.4171539961014, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_BACV8 (A6L5G8)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300554.1
- ProteinModelPortal:   A6L5G8
- STRING:   A6L5G8
- GeneID:   5304267
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_3306
- NMPDR:   fig|435590.6.peg.3082
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK2516787
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75172; Mature: 75041

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 112-112 BINDING 110-110 BINDING 133-133 BINDING 170-170 BINDING 285-285 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIERIDQLREELHRHNYNYYVLNAPEITDQEFDKLMRELQDLEEKHPEHRDENSPSMR
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VGSDINKNFTQVVHKYPMLSLANTYSETEVTEFYDRVKKSLNEDFEICCEMKYDGTSISL
CCCCCCHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEE
TYENGKLVRAVTRGDGVQGDDVTGNVKTIRSIPLVLHGKGYPETFEIRGEILMPWVVFEE
EECCCCEEEEEECCCCCCCCCCCCCHHHHEECCEEEECCCCCCEEEECCCEEEHHHHHHH
LNKEREAREEPLFANPRNAASGTLKLQNSAIVASRKLDAYLYYLLGDNLPCDGHYENLKE
HHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEEHHHHHEEEEEECCCCCCCCCHHHHHH
AEKWGFKISDLTRKCKTLQEVFDFIKYWDVERKNLPVATDGIVLKVNSLRQQRNLGFTAK
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEHHHHHHHCCCCCCC
SPRWAIAYKFQAEQALTRLNKVTYQVGRTGAVTPVANLDPIQLSGTVVKRASLHNADIIE
CCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHCCCCCHHHC
GLDLHIGDMVYVEKGGEIIPKIVGVDKDARIMIGDKVKFITHCPECGSKLVRYEGEAAYY
CCCCCCCCEEEEECCCCCCHHHHCCCCCCEEEECCCCCEECCCHHHHHHHHEECCCEEEE
CTNDAACPPQIKGKIEHFISRRAMNIDGLGPETVDQFYQEGLIRDVADLYTLKTSDIINL
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ERMGEKSAENIIKGIEQSKEVPFERVLFALGIRFVGETVAKKVAKSFKSMDALADASLDN
HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHC
LIHVDEIGEKIAQSILLYFANPKNRDIIERLRVAGVRLEADEEDTSEHTDKLAGKSIVIS
EEEHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCEEEECCCCCHHHHHHHHCCCEEEEE
GVFAHHSRDEYKELIEKHGGKNVGSISSKTSFILAGDNMGPSKLEKAQKLGVTIVSEEEF
EHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEECHHHH
LQMIE
HHHCC
>Mature Secondary Structure 
TEIERIDQLREELHRHNYNYYVLNAPEITDQEFDKLMRELQDLEEKHPEHRDENSPSMR
CHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VGSDINKNFTQVVHKYPMLSLANTYSETEVTEFYDRVKKSLNEDFEICCEMKYDGTSISL
CCCCCCHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEE
TYENGKLVRAVTRGDGVQGDDVTGNVKTIRSIPLVLHGKGYPETFEIRGEILMPWVVFEE
EECCCCEEEEEECCCCCCCCCCCCCHHHHEECCEEEECCCCCCEEEECCCEEEHHHHHHH
LNKEREAREEPLFANPRNAASGTLKLQNSAIVASRKLDAYLYYLLGDNLPCDGHYENLKE
HHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEEHHHHHEEEEEECCCCCCCCCHHHHHH
AEKWGFKISDLTRKCKTLQEVFDFIKYWDVERKNLPVATDGIVLKVNSLRQQRNLGFTAK
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEHHHHHHHCCCCCCC
SPRWAIAYKFQAEQALTRLNKVTYQVGRTGAVTPVANLDPIQLSGTVVKRASLHNADIIE
CCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHCCCCCHHHC
GLDLHIGDMVYVEKGGEIIPKIVGVDKDARIMIGDKVKFITHCPECGSKLVRYEGEAAYY
CCCCCCCCEEEEECCCCCCHHHHCCCCCCEEEECCCCCEECCCHHHHHHHHEECCCEEEE
CTNDAACPPQIKGKIEHFISRRAMNIDGLGPETVDQFYQEGLIRDVADLYTLKTSDIINL
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ERMGEKSAENIIKGIEQSKEVPFERVLFALGIRFVGETVAKKVAKSFKSMDALADASLDN
HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHC
LIHVDEIGEKIAQSILLYFANPKNRDIIERLRVAGVRLEADEEDTSEHTDKLAGKSIVIS
EEEHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCEEEECCCCCHHHHHHHHCCCEEEEE
GVFAHHSRDEYKELIEKHGGKNVGSISSKTSFILAGDNMGPSKLEKAQKLGVTIVSEEEF
EHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEECHHHH
LQMIE
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA