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The map label for this gene is otsA [H]

Identifier: 150005766

GI number: 150005766

Start: 4088975

End: 4091227

Strand: Reverse

Name: otsA [H]

Synonym: BVU_3259

Alternate gene names: 150005766

Gene position: 4091227-4088975 (Counterclockwise)

Preceding gene: 150005770

Following gene: 150005765

Centisome position: 79.24

GC content: 43.05

Gene sequence:

>2253_bases
ATGAAATTGTATATCATATCAAACAGACTGCCCGTGAAGGCTGTCGCAGAACAAGATACCTTTGTTTTCTCACGTAGTGA
AGGAGGTCTGACAACGGGCCTAAATTCCCTACAAGGCAATTACGAGAAACATTGGGTGGGGTGGCCCGGAATCTGTACAG
ACAAAGAGGAAGAAAAACAGGATATCTGTCACCGGTTGGAGGAAATGAATCTCCATCCCATATTTTTATCCGATGAGCAA
TATAAGAACTATTATGAGGGATACAGTAACAGTACCCTGTGGCCTTTATGTCACTATTTCTTTGCCTATACATTATATAG
AAAGAGTTTCTGGCAATCTTACCAAGAGGTTAACGCTTTGTTTTGTCGGGAAATCATCCGCCTTGTGGAACCGGATGATT
GGGTCTGGGTACAAGATTATCAGTTGATGTTACTTCCGGAAATGCTCCGCCAAGAGCTTCCCCGGTTGCATATCGGCTAT
TTCCATCACATTCCGTTTCCATCTTATGAGTTATTCCGAATTCTGCCTGAACGTGCCGAAATACTGAAAGGATTGTTAGG
AGCCGATTTTATCGCATTTCATACACATGACTATATGCGTCATTTCATCAGCGCGGCCGAACGGGTACTCCATATGGATT
TCAGTTTGGATGAAACCCGGATAGGCAGTCGCATTGTGCGCGTTGATGCACTTCCGATGGGGATAAATTATGACCTTTAC
CACAATGTGTCACAACAAAAAAATGTTTGGAAAGCTATAGAACGAACCCGTCTCTTGTTTGGCAAACATAAACTTATCCT
ATCGGTAGACCGTTTGGATTATAGTAAAGGCATTTTGCACCGTCTTTATGGATTTGCCTCTTTTTTGGAACACCATCCCG
AATATCATGGCAAGGTAACACTGGCTATGGTCATAGTTCCTTCACGTGATCATGTAGGCAGTTATGCCGAATTGAAAACC
CGGATTGACGAGGAAATAGGTTCTATCAACGGACGATATTCTACAATGAACTGGACTCCGGTCTGCTATTTTTATCATGG
TTTCTCATTCGAAGAATTGGCTGCCATGTATTTTATAGCTGATATTGCATTAGTCACTCCTTTGCGTGACGGAATGAACT
TGGTTGCCAAAGAGTATATTGCTGTCAAGCAAGACAATCCCGGTGTATTGGTCTTAAGTGAAATGGCCGGTGCCGCTGTG
GAACTGACCGACGCCTTGCTGGTTAATCCCAATGATACGGAGCAGATAGAAAATGCCATTTGCCGGGCATTGGAAATGCC
GTTTGAAGAACAGAAAGAACGGATGCACCGTATGCAGTCTATTGTATCCGTACAGACTGTCAATAAATGGGCTGCCGATT
TTGTAAATGAATGGCAGGAAGTGGCACACAAAAACAAAACCATGCTTCTTAAGAAGATAGGATCACAGAATATGCAGGAA
ATTCAGCATCAATACTTACATGCTAAAAAGCGTTTGATTTTATTGGATTATGACGGGACGCTGGTTCCTTTTCAGAAAAG
GCCGGAAGATGCCTCTCCTACTCCGCAATTGCTGGATACATTGCAGAAACTGACTGCCGACCCCTTGAATCATGTGGTAA
TAAACAGCGGACGTGACCATTTTACTTTAGAGAAATGGTTGGGTGCACTACCTATTTCCTTTGCAGCCGAACATGGGGCC
TTTTATAAAGAGAATGGTGTATGGCACAAGAATGTACACGCGCAGGAATGGAGCCCCGGATTACTTTCTATATTAAAATT
ATTTGTCAGCAAGACTCCTCGCTCCCATCTTGAAGTCAAAGAAACGGCACTTGCCTGGCATTATCGTGAGACCGATGCAT
GGTTGGGCAGATTACGTGCGCAACAGTTGGTCAATTCCTTGATATCTATTTGCTTGAAACAGAATTTGCAAATCATGCAA
GGTAATAAGGTGATAGAAATAAAATCTCCCGAATTTACCAAAGGTTCAGAAGTAAACAGGCTTTTGCTTGCCACCCGATA
TGATTTTATCCTGGCAATGGGAGATGATACCACGGATGATGATATGTTTAAAGCCCTGCCTGTTACTGCTGTGACTGTAA
AGATTGGTACAGCATCCGAGAGTGCACGTTACAATCTGCCCGTACAGACAGACACCTTGCCGTTTTTACAACGGCTGACA
GATAAAAGCGTGGTGAAAGCAGCCTTGAAAAGTGGTTTGAAAGGGCAGCTATCATCGGCTATAGATTTTTTAAAACGGAT
AATAAACCACTAA

Upstream 100 bases:

>100_bases
TGTATAAACTAATTGACAAGTATATATCATATTGATAATAAGATATTTAAGTTGGTGGTATGACTTTTGTCATCTAACTG
AAAAAAAGAATTATATAAAA

Downstream 100 bases:

>100_bases
CATTACAAAAAAATAACCATGGACAATTTGAATTACGGTGTAATCGGTAATTGCTGCACAGCCGCACTGATTTCCGAAAA
AGGAAATATTGAATGGCTAT

Product: putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB

Products: H+; alpha,alpha'-Trehalose 6-phosphate; UDP [C]

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 750; Mature: 750

Protein sequence:

>750_residues
MKLYIISNRLPVKAVAEQDTFVFSRSEGGLTTGLNSLQGNYEKHWVGWPGICTDKEEEKQDICHRLEEMNLHPIFLSDEQ
YKNYYEGYSNSTLWPLCHYFFAYTLYRKSFWQSYQEVNALFCREIIRLVEPDDWVWVQDYQLMLLPEMLRQELPRLHIGY
FHHIPFPSYELFRILPERAEILKGLLGADFIAFHTHDYMRHFISAAERVLHMDFSLDETRIGSRIVRVDALPMGINYDLY
HNVSQQKNVWKAIERTRLLFGKHKLILSVDRLDYSKGILHRLYGFASFLEHHPEYHGKVTLAMVIVPSRDHVGSYAELKT
RIDEEIGSINGRYSTMNWTPVCYFYHGFSFEELAAMYFIADIALVTPLRDGMNLVAKEYIAVKQDNPGVLVLSEMAGAAV
ELTDALLVNPNDTEQIENAICRALEMPFEEQKERMHRMQSIVSVQTVNKWAADFVNEWQEVAHKNKTMLLKKIGSQNMQE
IQHQYLHAKKRLILLDYDGTLVPFQKRPEDASPTPQLLDTLQKLTADPLNHVVINSGRDHFTLEKWLGALPISFAAEHGA
FYKENGVWHKNVHAQEWSPGLLSILKLFVSKTPRSHLEVKETALAWHYRETDAWLGRLRAQQLVNSLISICLKQNLQIMQ
GNKVIEIKSPEFTKGSEVNRLLLATRYDFILAMGDDTTDDDMFKALPVTAVTVKIGTASESARYNLPVQTDTLPFLQRLT
DKSVVKAALKSGLKGQLSSAIDFLKRIINH

Sequences:

>Translated_750_residues
MKLYIISNRLPVKAVAEQDTFVFSRSEGGLTTGLNSLQGNYEKHWVGWPGICTDKEEEKQDICHRLEEMNLHPIFLSDEQ
YKNYYEGYSNSTLWPLCHYFFAYTLYRKSFWQSYQEVNALFCREIIRLVEPDDWVWVQDYQLMLLPEMLRQELPRLHIGY
FHHIPFPSYELFRILPERAEILKGLLGADFIAFHTHDYMRHFISAAERVLHMDFSLDETRIGSRIVRVDALPMGINYDLY
HNVSQQKNVWKAIERTRLLFGKHKLILSVDRLDYSKGILHRLYGFASFLEHHPEYHGKVTLAMVIVPSRDHVGSYAELKT
RIDEEIGSINGRYSTMNWTPVCYFYHGFSFEELAAMYFIADIALVTPLRDGMNLVAKEYIAVKQDNPGVLVLSEMAGAAV
ELTDALLVNPNDTEQIENAICRALEMPFEEQKERMHRMQSIVSVQTVNKWAADFVNEWQEVAHKNKTMLLKKIGSQNMQE
IQHQYLHAKKRLILLDYDGTLVPFQKRPEDASPTPQLLDTLQKLTADPLNHVVINSGRDHFTLEKWLGALPISFAAEHGA
FYKENGVWHKNVHAQEWSPGLLSILKLFVSKTPRSHLEVKETALAWHYRETDAWLGRLRAQQLVNSLISICLKQNLQIMQ
GNKVIEIKSPEFTKGSEVNRLLLATRYDFILAMGDDTTDDDMFKALPVTAVTVKIGTASESARYNLPVQTDTLPFLQRLT
DKSVVKAALKSGLKGQLSSAIDFLKRIINH
>Mature_750_residues
MKLYIISNRLPVKAVAEQDTFVFSRSEGGLTTGLNSLQGNYEKHWVGWPGICTDKEEEKQDICHRLEEMNLHPIFLSDEQ
YKNYYEGYSNSTLWPLCHYFFAYTLYRKSFWQSYQEVNALFCREIIRLVEPDDWVWVQDYQLMLLPEMLRQELPRLHIGY
FHHIPFPSYELFRILPERAEILKGLLGADFIAFHTHDYMRHFISAAERVLHMDFSLDETRIGSRIVRVDALPMGINYDLY
HNVSQQKNVWKAIERTRLLFGKHKLILSVDRLDYSKGILHRLYGFASFLEHHPEYHGKVTLAMVIVPSRDHVGSYAELKT
RIDEEIGSINGRYSTMNWTPVCYFYHGFSFEELAAMYFIADIALVTPLRDGMNLVAKEYIAVKQDNPGVLVLSEMAGAAV
ELTDALLVNPNDTEQIENAICRALEMPFEEQKERMHRMQSIVSVQTVNKWAADFVNEWQEVAHKNKTMLLKKIGSQNMQE
IQHQYLHAKKRLILLDYDGTLVPFQKRPEDASPTPQLLDTLQKLTADPLNHVVINSGRDHFTLEKWLGALPISFAAEHGA
FYKENGVWHKNVHAQEWSPGLLSILKLFVSKTPRSHLEVKETALAWHYRETDAWLGRLRAQQLVNSLISICLKQNLQIMQ
GNKVIEIKSPEFTKGSEVNRLLLATRYDFILAMGDDTTDDDMFKALPVTAVTVKIGTASESARYNLPVQTDTLPFLQRLT
DKSVVKAALKSGLKGQLSSAIDFLKRIINH

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=484, Percent_Identity=31.8181818181818, Blast_Score=254, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI71987012, Length=423, Percent_Identity=27.4231678486998, Blast_Score=160, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI115533558, Length=445, Percent_Identity=27.1910112359551, Blast_Score=152, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI115533560, Length=445, Percent_Identity=27.1910112359551, Blast_Score=151, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319602, Length=462, Percent_Identity=40.4761904761905, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6320279, Length=667, Percent_Identity=32.9835082458771, Blast_Score=343, Evalue=4e-95,
Organism=Saccharomyces cerevisiae, GI6323537, Length=580, Percent_Identity=30.1724137931034, Blast_Score=267, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6323917, Length=585, Percent_Identity=29.0598290598291, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI19920676, Length=755, Percent_Identity=31.523178807947, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI221473368, Length=240, Percent_Identity=26.25, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI20129307, Length=240, Percent_Identity=26.25, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24582490, Length=240, Percent_Identity=26.25, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15 [C]

Molecular weight: Translated: 86539; Mature: 86539

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYIISNRLPVKAVAEQDTFVFSRSEGGLTTGLNSLQGNYEKHWVGWPGICTDKEEEKQ
CEEEEEECCCCCCEECCCCCEEEECCCCCHHHCHHHHCCCCCCEECCCCCCCCCCHHHHH
DICHRLEEMNLHPIFLSDEQYKNYYEGYSNSTLWPLCHYFFAYTLYRKSFWQSYQEVNAL
HHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FCREIIRLVEPDDWVWVQDYQLMLLPEMLRQELPRLHIGYFHHIPFPSYELFRILPERAE
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCEECCEEECCCCCCHHHHHHHHHHHH
ILKGLLGADFIAFHTHDYMRHFISAAERVLHMDFSLDETRIGSRIVRVDALPMGINYDLY
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEEEEECCCCCHHH
HNVSQQKNVWKAIERTRLLFGKHKLILSVDRLDYSKGILHRLYGFASFLEHHPEYHGKVT
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEE
LAMVIVPSRDHVGSYAELKTRIDEEIGSINGRYSTMNWTPVCYFYHGFSFEELAAMYFIA
EEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHH
DIALVTPLRDGMNLVAKEYIAVKQDNPGVLVLSEMAGAAVELTDALLVNPNDTEQIENAI
HHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCEEEEEEEEECCCCHHHHHHHH
CRALEMPFEEQKERMHRMQSIVSVQTVNKWAADFVNEWQEVAHKNKTMLLKKIGSQNMQE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHH
IQHQYLHAKKRLILLDYDGTLVPFQKRPEDASPTPQLLDTLQKLTADPLNHVVINSGRDH
HHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCC
FTLEKWLGALPISFAAEHGAFYKENGVWHKNVHAQEWSPGLLSILKLFVSKTPRSHLEVK
EEHHHHHCCCCHHHHHHCCCEECCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
ETALAWHYRETDAWLGRLRAQQLVNSLISICLKQNLQIMQGNKVIEIKSPEFTKGSEVNR
HHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCCCCCHHH
LLLATRYDFILAMGDDTTDDDMFKALPVTAVTVKIGTASESARYNLPVQTDTLPFLQRLT
HHEEEEEEEEEEECCCCCCCHHHHHCCEEEEEEEEECCCCCCEECCCCCCCHHHHHHHHH
DKSVVKAALKSGLKGQLSSAIDFLKRIINH
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLYIISNRLPVKAVAEQDTFVFSRSEGGLTTGLNSLQGNYEKHWVGWPGICTDKEEEKQ
CEEEEEECCCCCCEECCCCCEEEECCCCCHHHCHHHHCCCCCCEECCCCCCCCCCHHHHH
DICHRLEEMNLHPIFLSDEQYKNYYEGYSNSTLWPLCHYFFAYTLYRKSFWQSYQEVNAL
HHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FCREIIRLVEPDDWVWVQDYQLMLLPEMLRQELPRLHIGYFHHIPFPSYELFRILPERAE
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCEECCEEECCCCCCHHHHHHHHHHHH
ILKGLLGADFIAFHTHDYMRHFISAAERVLHMDFSLDETRIGSRIVRVDALPMGINYDLY
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEEEEECCCCCHHH
HNVSQQKNVWKAIERTRLLFGKHKLILSVDRLDYSKGILHRLYGFASFLEHHPEYHGKVT
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEE
LAMVIVPSRDHVGSYAELKTRIDEEIGSINGRYSTMNWTPVCYFYHGFSFEELAAMYFIA
EEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHH
DIALVTPLRDGMNLVAKEYIAVKQDNPGVLVLSEMAGAAVELTDALLVNPNDTEQIENAI
HHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCEEEEEEEEECCCCHHHHHHHH
CRALEMPFEEQKERMHRMQSIVSVQTVNKWAADFVNEWQEVAHKNKTMLLKKIGSQNMQE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHH
IQHQYLHAKKRLILLDYDGTLVPFQKRPEDASPTPQLLDTLQKLTADPLNHVVINSGRDH
HHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCC
FTLEKWLGALPISFAAEHGAFYKENGVWHKNVHAQEWSPGLLSILKLFVSKTPRSHLEVK
EEHHHHHCCCCHHHHHHCCCEECCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
ETALAWHYRETDAWLGRLRAQQLVNSLISICLKQNLQIMQGNKVIEIKSPEFTKGSEVNR
HHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCCCCCHHH
LLLATRYDFILAMGDDTTDDDMFKALPVTAVTVKIGTASESARYNLPVQTDTLPFLQRLT
HHEEEEEEEEEEECCCCCCCHHHHHCCEEEEEEEEECCCCCCEECCCCCCCHHHHHHHHH
DKSVVKAALKSGLKGQLSSAIDFLKRIINH
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Glucose 6-phosphate; UDPglucose [C]

Specific reaction: D-Glucose 6-phosphate + UDPglucose --> H+ + alpha,alpha'-Trehalose 6-phosphate + UDP [C]

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA