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The map label for this gene is cobS [H]

Identifier: 150005659

GI number: 150005659

Start: 3967862

End: 3968611

Strand: Reverse

Name: cobS [H]

Synonym: BVU_3149

Alternate gene names: 150005659

Gene position: 3968611-3967862 (Counterclockwise)

Preceding gene: 150005660

Following gene: 150005658

Centisome position: 76.86

GC content: 44.8

Gene sequence:

>750_bases
ATGAAACTGCTTGCCTCCCTCATTTTTTTCACACGCCTTCCGTTTTGGAAAATAGCTGACGTACCACCTGTTTACTTTAA
ACGAGTAGTGGACTACTGGCCCTTTGTTGGATGGCTGACAGGAGGTATCATGGCAGGCACTCTATGGATCACAGCAGAAT
TTCTGCCTGTACAAATAGCTGTGCTCATGGCGTTGGCTGCCCGGTTATTAGTAACAGGTGCTTTACACGAAGATGGATTG
GCAGACTTCTGCGATGGCTTCGGGGGCGGTACATCACGTGACAAGATTCTGTCCATCATGAAGGATTCACATATCGGCAC
ATACGGAGTGCTCGGATTATTATTCTATTATGGATTGATGTGGAATATACTCACCACACTTTCTGTACCCTTGGCATGTG
CGGCCATATTAAGCGGAGATGCCTGGAGCAAGTTTTGTGCCGCACAAATCATCAACACGCTTCCATACGCCCGTAAAGAA
GAAGAATCGAAAGCAAAAGTCATATATGACCGTATGAGCCCAGATGTTTTATTATCCGCTTTTATGGCCGGAGCCCTACC
TATGCTTTTATTACTGGATAAAGGATACTGGTGGGCAGCCATCGCTCCTGCTATGATGTTCATCCTTTTAATGTCACTCA
TGCGCAGACGCCTGCAAGGATATACCGGTGATTGTTGCGGAGCGACTTTCCTATTATGTGAGTTTTCTTTTTATCTGACT
ATAAACTTAATCTATACAAATCTATGGTAA

Upstream 100 bases:

>100_bases
CAGGAGCGATATGCGCTTATCCCATCGTTGTTTCCTCTGTAAACATGATGAATGAGATGGACAATTTCGCCCATGCCTCC
ATCACTAAATATTTTTAACC

Downstream 100 bases:

>100_bases
TATACTTCATGCGTCATACTGCTGTTGATGTTCCGCAAGGCGTTTGTTACGGACAGACGGATGTACCCTTAAAGCCGACT
TTTGAAATGGAGGCAACTCA

Product: cobalamin 5'-phosphate synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKLLASLIFFTRLPFWKIADVPPVYFKRVVDYWPFVGWLTGGIMAGTLWITAEFLPVQIAVLMALAARLLVTGALHEDGL
ADFCDGFGGGTSRDKILSIMKDSHIGTYGVLGLLFYYGLMWNILTTLSVPLACAAILSGDAWSKFCAAQIINTLPYARKE
EESKAKVIYDRMSPDVLLSAFMAGALPMLLLLDKGYWWAAIAPAMMFILLMSLMRRRLQGYTGDCCGATFLLCEFSFYLT
INLIYTNLW

Sequences:

>Translated_249_residues
MKLLASLIFFTRLPFWKIADVPPVYFKRVVDYWPFVGWLTGGIMAGTLWITAEFLPVQIAVLMALAARLLVTGALHEDGL
ADFCDGFGGGTSRDKILSIMKDSHIGTYGVLGLLFYYGLMWNILTTLSVPLACAAILSGDAWSKFCAAQIINTLPYARKE
EESKAKVIYDRMSPDVLLSAFMAGALPMLLLLDKGYWWAAIAPAMMFILLMSLMRRRLQGYTGDCCGATFLLCEFSFYLT
INLIYTNLW
>Mature_249_residues
MKLLASLIFFTRLPFWKIADVPPVYFKRVVDYWPFVGWLTGGIMAGTLWITAEFLPVQIAVLMALAARLLVTGALHEDGL
ADFCDGFGGGTSRDKILSIMKDSHIGTYGVLGLLFYYGLMWNILTTLSVPLACAAILSGDAWSKFCAAQIINTLPYARKE
EESKAKVIYDRMSPDVLLSAFMAGALPMLLLLDKGYWWAAIAPAMMFILLMSLMRRRLQGYTGDCCGATFLLCEFSFYLT
INLIYTNLW

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=244, Percent_Identity=31.1475409836066, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 27795; Mature: 27795

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCC
>Mature Secondary Structure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HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA