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The map label for this gene is pycA [H]

Identifier: 150005616

GI number: 150005616

Start: 3924630

End: 3926141

Strand: Reverse

Name: pycA [H]

Synonym: BVU_3104

Alternate gene names: 150005616

Gene position: 3926141-3924630 (Counterclockwise)

Preceding gene: 150005619

Following gene: 150005615

Centisome position: 76.04

GC content: 49.8

Gene sequence:

>1512_bases
ATGATAAAGAAAGTATTGATTGCAAACAGAGGTGAAATAGCCTTACGTGTAATGCGTTCCTGCCGAGAAAAGGGCATACG
GACCGTTGCTGTCTTTTCAGAAGCCGACCGCACTTCTCATCATGTGATGTATGCTGACGAGGCCTATCTTATAGGTCCTG
CTATAGCCAGGGAAAGCTATTTAAATATAGAAAAAGTAATAGAGACTGCCAAACGATGCAAAGCCGATGCCATTCATCCC
GGTTATGGTTTTTTGTCCGAGAATGCTGATTTTGCCCGCCGGTGCAAAGAGGAGGGGATCATCTTTATCGGCCCTTCCGC
CGAGACGATGGAAGCGATGGGTGACAAGATTGCCGCCCGTAAGCGTATGATTGCCGCAGGCGTTCCCGTGGTCCCCGGTA
CGGAACAGCCGTTGCAAAGTGCCGAAGAGGCGGTACGTATCTGTAATGAAATAGGCTATCCTGTCATGCTGAAGGCTTCT
ATGGGAGGCGGCGGTAAAGGGATGAGGCTGATACACAGTGAAGACGAAGTGGTGGAGGCATATAACACCGCCCGTTCCGA
GTCCATGTCTTCTTTCGGGGATGATACGGTTTATCTGGAAAAATTTGTGGAGGAACCCCATCATATAGAATTTCAAATTC
TGGGTGACAATCATGGCAATGTAATCCATCTGTTTGACCGCGAGTGTTCTGTCCAGCGTCGCAACCAGAAGATTGTAGAG
GAGAGCCCGTCTCCGTTCCTTACTCCGGAACTCCGCCAGGAGATGGGGGAAAAGGCAGTGGCTGCCGCCAGGGCGGTGAA
CTATTCGGGAGCCGGAACTATCGAGTTTCTGGTGGACAAGAACCGCGATTTCTATTTTCTGGAGATGAACACCCGTTTGC
AGGTGGAGCATCCCATCACCGAAGAAGTGGTGGGCGTGGATCTGGTAAAAGAACAAATCCGTATAGCCAATAACGAGGTG
CTGCACCTGAAGCAAGAAGAACTTTTCCAGCGCGGTCATGCCATCGAATGTCGTATCTGTGCGGAAGACACGGAGAACAA
CTTCATGCCTTCGCCGGGTATCATCAAACAGCTGACGGAGCCTAACGGCATCGGCGTACGCATTGACAGCTACGTTTACG
AAGGTTACGAGATTCCCATCTATTACGACCCGATGATAGGCAAGCTGATTGTATGGGCTACGACCCGCCAATATGCCATA
GAACGTATGCGCCGGGTACTTTACGAGTACAAGATTACGGGCCTGAAGACCAACCTCAGTTATCTGAAACGCATCATGCA
TACCCCCGACTTTGTAAAGGGTGAGTATAATACCTTGTTCATAGAGAAGAACTCGCGTATGCTGCTCCGTGGCAACGGTA
ATAATGAGGAGATTGAGAATATCACCATGATCGCCTCTTATATTGACTATCTGATGAATCTGGAAGAGAACAAGAGTCCC
CAGCTGTCTGATGCCCGTCCCATCAGCCGCTGGCGTGAGTTCGGCTTGCAGAAAGGAGTATTGAGAATCTGA

Upstream 100 bases:

>100_bases
ATGGAGAATTATTCCGATTGCCTGTGCCATGACTGCCTGAAAGCCGTCAAGGACAGTTTCTATGCCTGCGAAATTAACCC
CCGTTATAATAAGAAGAAAA

Downstream 100 bases:

>100_bases
TTCTTCATTTAATTAAGCAAAAGTATGGAAATACATATTGGAGACCGCGTAGCCGATGTGACGTTGGTGAGCAAGGAAGG
AAACAAGGTACAGTTTATGA

Product: pyruvate carboxylase subunit A

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MIKKVLIANRGEIALRVMRSCREKGIRTVAVFSEADRTSHHVMYADEAYLIGPAIARESYLNIEKVIETAKRCKADAIHP
GYGFLSENADFARRCKEEGIIFIGPSAETMEAMGDKIAARKRMIAAGVPVVPGTEQPLQSAEEAVRICNEIGYPVMLKAS
MGGGGKGMRLIHSEDEVVEAYNTARSESMSSFGDDTVYLEKFVEEPHHIEFQILGDNHGNVIHLFDRECSVQRRNQKIVE
ESPSPFLTPELRQEMGEKAVAAARAVNYSGAGTIEFLVDKNRDFYFLEMNTRLQVEHPITEEVVGVDLVKEQIRIANNEV
LHLKQEELFQRGHAIECRICAEDTENNFMPSPGIIKQLTEPNGIGVRIDSYVYEGYEIPIYYDPMIGKLIVWATTRQYAI
ERMRRVLYEYKITGLKTNLSYLKRIMHTPDFVKGEYNTLFIEKNSRMLLRGNGNNEEIENITMIASYIDYLMNLEENKSP
QLSDARPISRWREFGLQKGVLRI

Sequences:

>Translated_503_residues
MIKKVLIANRGEIALRVMRSCREKGIRTVAVFSEADRTSHHVMYADEAYLIGPAIARESYLNIEKVIETAKRCKADAIHP
GYGFLSENADFARRCKEEGIIFIGPSAETMEAMGDKIAARKRMIAAGVPVVPGTEQPLQSAEEAVRICNEIGYPVMLKAS
MGGGGKGMRLIHSEDEVVEAYNTARSESMSSFGDDTVYLEKFVEEPHHIEFQILGDNHGNVIHLFDRECSVQRRNQKIVE
ESPSPFLTPELRQEMGEKAVAAARAVNYSGAGTIEFLVDKNRDFYFLEMNTRLQVEHPITEEVVGVDLVKEQIRIANNEV
LHLKQEELFQRGHAIECRICAEDTENNFMPSPGIIKQLTEPNGIGVRIDSYVYEGYEIPIYYDPMIGKLIVWATTRQYAI
ERMRRVLYEYKITGLKTNLSYLKRIMHTPDFVKGEYNTLFIEKNSRMLLRGNGNNEEIENITMIASYIDYLMNLEENKSP
QLSDARPISRWREFGLQKGVLRI
>Mature_503_residues
MIKKVLIANRGEIALRVMRSCREKGIRTVAVFSEADRTSHHVMYADEAYLIGPAIARESYLNIEKVIETAKRCKADAIHP
GYGFLSENADFARRCKEEGIIFIGPSAETMEAMGDKIAARKRMIAAGVPVVPGTEQPLQSAEEAVRICNEIGYPVMLKAS
MGGGGKGMRLIHSEDEVVEAYNTARSESMSSFGDDTVYLEKFVEEPHHIEFQILGDNHGNVIHLFDRECSVQRRNQKIVE
ESPSPFLTPELRQEMGEKAVAAARAVNYSGAGTIEFLVDKNRDFYFLEMNTRLQVEHPITEEVVGVDLVKEQIRIANNEV
LHLKQEELFQRGHAIECRICAEDTENNFMPSPGIIKQLTEPNGIGVRIDSYVYEGYEIPIYYDPMIGKLIVWATTRQYAI
ERMRRVLYEYKITGLKTNLSYLKRIMHTPDFVKGEYNTLFIEKNSRMLLRGNGNNEEIENITMIASYIDYLMNLEENKSP
QLSDARPISRWREFGLQKGVLRI

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI116805327, Length=449, Percent_Identity=53.2293986636971, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI189095269, Length=440, Percent_Identity=52.0454545454545, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI65506442, Length=440, Percent_Identity=52.0454545454545, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI295821183, Length=440, Percent_Identity=52.0454545454545, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI106049528, Length=452, Percent_Identity=46.9026548672566, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI106049295, Length=452, Percent_Identity=46.9026548672566, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI106049292, Length=452, Percent_Identity=46.9026548672566, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI38679960, Length=503, Percent_Identity=32.4055666003976, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI38679977, Length=503, Percent_Identity=32.4055666003976, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI38679967, Length=503, Percent_Identity=32.4055666003976, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI38679971, Length=503, Percent_Identity=32.4055666003976, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI38679974, Length=503, Percent_Identity=32.4055666003976, Blast_Score=252, Evalue=6e-67,
Organism=Homo sapiens, GI134142062, Length=503, Percent_Identity=31.8091451292247, Blast_Score=239, Evalue=6e-63,
Organism=Escherichia coli, GI1789654, Length=444, Percent_Identity=52.9279279279279, Blast_Score=462, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI71987519, Length=449, Percent_Identity=50.5567928730512, Blast_Score=445, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17562816, Length=493, Percent_Identity=44.2190669371197, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17567343, Length=440, Percent_Identity=47.5, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI133931226, Length=487, Percent_Identity=30.1848049281314, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI71997163, Length=514, Percent_Identity=30.1556420233463, Blast_Score=221, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI71997168, Length=514, Percent_Identity=30.1556420233463, Blast_Score=221, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6319695, Length=523, Percent_Identity=42.0650095602294, Blast_Score=402, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321376, Length=523, Percent_Identity=42.0650095602294, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6319685, Length=439, Percent_Identity=43.0523917995444, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324343, Length=510, Percent_Identity=30.1960784313725, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6323863, Length=485, Percent_Identity=31.5463917525773, Blast_Score=226, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6322331, Length=304, Percent_Identity=21.7105263157895, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24651757, Length=450, Percent_Identity=49.3333333333333, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24652212, Length=452, Percent_Identity=46.6814159292035, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24652210, Length=452, Percent_Identity=46.6814159292035, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24652214, Length=452, Percent_Identity=46.6814159292035, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI19921944, Length=452, Percent_Identity=46.6814159292035, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24652216, Length=452, Percent_Identity=46.6814159292035, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI281363050, Length=452, Percent_Identity=46.6814159292035, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24652224, Length=452, Percent_Identity=46.6814159292035, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24652222, Length=452, Percent_Identity=46.6814159292035, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24652220, Length=452, Percent_Identity=46.6814159292035, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24652218, Length=452, Percent_Identity=46.6814159292035, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24651759, Length=411, Percent_Identity=48.1751824817518, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI161076409, Length=497, Percent_Identity=31.7907444668008, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24586458, Length=497, Percent_Identity=31.7907444668008, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI161076407, Length=497, Percent_Identity=31.7907444668008, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24586460, Length=497, Percent_Identity=31.7907444668008, Blast_Score=242, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 57205; Mature: 57205

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKVLIANRGEIALRVMRSCREKGIRTVAVFSEADRTSHHVMYADEAYLIGPAIARESY
CCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCEEEECHHHHHHHH
LNIEKVIETAKRCKADAIHPGYGFLSENADFARRCKEEGIIFIGPSAETMEAMGDKIAAR
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHHHH
KRMIAAGVPVVPGTEQPLQSAEEAVRICNEIGYPVMLKASMGGGGKGMRLIHSEDEVVEA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCHHHHHH
YNTARSESMSSFGDDTVYLEKFVEEPHHIEFQILGDNHGNVIHLFDRECSVQRRNQKIVE
HHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHH
ESPSPFLTPELRQEMGEKAVAAARAVNYSGAGTIEFLVDKNRDFYFLEMNTRLQVEHPIT
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCEEEECCCCH
EEVVGVDLVKEQIRIANNEVLHLKQEELFQRGHAIECRICAEDTENNFMPSPGIIKQLTE
HHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHCCC
PNGIGVRIDSYVYEGYEIPIYYDPMIGKLIVWATTRQYAIERMRRVLYEYKITGLKTNLS
CCCCEEEEEEEEECCEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCEECCHH
YLKRIMHTPDFVKGEYNTLFIEKNSRMLLRGNGNNEEIENITMIASYIDYLMNLEENKSP
HHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCC
QLSDARPISRWREFGLQKGVLRI
CCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIKKVLIANRGEIALRVMRSCREKGIRTVAVFSEADRTSHHVMYADEAYLIGPAIARESY
CCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCEEEECHHHHHHHH
LNIEKVIETAKRCKADAIHPGYGFLSENADFARRCKEEGIIFIGPSAETMEAMGDKIAAR
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHHHH
KRMIAAGVPVVPGTEQPLQSAEEAVRICNEIGYPVMLKASMGGGGKGMRLIHSEDEVVEA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCHHHHHH
YNTARSESMSSFGDDTVYLEKFVEEPHHIEFQILGDNHGNVIHLFDRECSVQRRNQKIVE
HHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHH
ESPSPFLTPELRQEMGEKAVAAARAVNYSGAGTIEFLVDKNRDFYFLEMNTRLQVEHPIT
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCEEEECCCCH
EEVVGVDLVKEQIRIANNEVLHLKQEELFQRGHAIECRICAEDTENNFMPSPGIIKQLTE
HHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHCCC
PNGIGVRIDSYVYEGYEIPIYYDPMIGKLIVWATTRQYAIERMRRVLYEYKITGLKTNLS
CCCCEEEEEEEEECCEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCEECCHH
YLKRIMHTPDFVKGEYNTLFIEKNSRMLLRGNGNNEEIENITMIASYIDYLMNLEENKSP
HHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCC
QLSDARPISRWREFGLQKGVLRI
CCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]