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The map label for this gene is eda [H]

Identifier: 150005571

GI number: 150005571

Start: 3867262

End: 3867936

Strand: Direct

Name: eda [H]

Synonym: BVU_3056

Alternate gene names: 150005571

Gene position: 3867262-3867936 (Clockwise)

Preceding gene: 150005570

Following gene: 150005572

Centisome position: 74.9

GC content: 46.81

Gene sequence:

>675_bases
ATGGCTCGTTTTGATAAAATAGCAGTGTTGGACAAGATTGGTTCAACCGGTATGGTTCCTGTATTCTACCATAAAGATGC
AGAAGTGGCAAAGAAAGTGGTGAAAGCTTGTTATGACGGCGGAGTTCGTGCATTCGAATTTACAAACCGTGGTGACTTTG
CTCATGAAGTGTTTGCGGAAGTTGTGAAATACGCGGCAAAGGAGTGTCCTGAAATGGCTATGGGAGTAGGTTCTATCGTT
GATCCTGCTACTGCCGCTCTTTACTTGCAATTGGGTGCTTGCTTTGTGGTAGGTCCGTTGTTCAATCCTGAAATCGCTAA
AGTGTGCAACCGTCGTTTGGTGGCTTATACTCCGGGATGCGGAAGTGTGTCCGAGGTAGGTTTCGCTCAAGAAGTAGGTT
GCGATCTTTGCAAAATATTCCCGGGTGATGTATTGGGAGCAAAATTGGTTAAAGGTCTGCTGGCTCCGATGCCTTGGTCT
AAACTGATGGTAACCGGTGGTGTGGAACCGACTCAGGAAAATCTGACTTCTTGGATTAAGGCAGGTGTGTTCTGTGTGGG
TATGGGTTCTAAATTGTTCCCTGGCGACAAGGTAGCCGCGGAAGACTGGGCGTATGTAACGGCAAAATGTAAAGAAGCAT
TGGGCTACATTGCGGAAGCCCGTGCAAATAAATAA

Upstream 100 bases:

>100_bases
TTATAGGAACACGAATGACGCAAATAACGCAAATTTTTGATACATGTTTTATTTGTATCATTTGTGTTCCTATCTTTCAT
TTAAACTAAAATATAACATT

Downstream 100 bases:

>100_bases
GAAAAAGATCAGCTCATGCCCGCAAGGGGTTAGTGAATTAGGGCGTGGGTATCATTTTGTTTAAAGGTGTATATAAAATG
GGTAAGGGCCATCGCGAAGT

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MARFDKIAVLDKIGSTGMVPVFYHKDAEVAKKVVKACYDGGVRAFEFTNRGDFAHEVFAEVVKYAAKECPEMAMGVGSIV
DPATAALYLQLGACFVVGPLFNPEIAKVCNRRLVAYTPGCGSVSEVGFAQEVGCDLCKIFPGDVLGAKLVKGLLAPMPWS
KLMVTGGVEPTQENLTSWIKAGVFCVGMGSKLFPGDKVAAEDWAYVTAKCKEALGYIAEARANK

Sequences:

>Translated_224_residues
MARFDKIAVLDKIGSTGMVPVFYHKDAEVAKKVVKACYDGGVRAFEFTNRGDFAHEVFAEVVKYAAKECPEMAMGVGSIV
DPATAALYLQLGACFVVGPLFNPEIAKVCNRRLVAYTPGCGSVSEVGFAQEVGCDLCKIFPGDVLGAKLVKGLLAPMPWS
KLMVTGGVEPTQENLTSWIKAGVFCVGMGSKLFPGDKVAAEDWAYVTAKCKEALGYIAEARANK
>Mature_223_residues
ARFDKIAVLDKIGSTGMVPVFYHKDAEVAKKVVKACYDGGVRAFEFTNRGDFAHEVFAEVVKYAAKECPEMAMGVGSIVD
PATAALYLQLGACFVVGPLFNPEIAKVCNRRLVAYTPGCGSVSEVGFAQEVGCDLCKIFPGDVLGAKLVKGLLAPMPWSK
LMVTGGVEPTQENLTSWIKAGVFCVGMGSKLFPGDKVAAEDWAYVTAKCKEALGYIAEARANK

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=188, Percent_Identity=29.7872340425532, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI48994954, Length=166, Percent_Identity=31.9277108433735, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 23918; Mature: 23787

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFDKIAVLDKIGSTGMVPVFYHKDAEVAKKVVKACYDGGVRAFEFTNRGDFAHEVFAE
CCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
VVKYAAKECPEMAMGVGSIVDPATAALYLQLGACFVVGPLFNPEIAKVCNRRLVAYTPGC
HHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCEEEECCCC
GSVSEVGFAQEVGCDLCKIFPGDVLGAKLVKGLLAPMPWSKLMVTGGVEPTQENLTSWIK
CCHHHCCCHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCHHHEEEECCCCCCHHHHHHHHH
AGVFCVGMGSKLFPGDKVAAEDWAYVTAKCKEALGYIAEARANK
HCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARFDKIAVLDKIGSTGMVPVFYHKDAEVAKKVVKACYDGGVRAFEFTNRGDFAHEVFAE
CCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
VVKYAAKECPEMAMGVGSIVDPATAALYLQLGACFVVGPLFNPEIAKVCNRRLVAYTPGC
HHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCEEEECCCC
GSVSEVGFAQEVGCDLCKIFPGDVLGAKLVKGLLAPMPWSKLMVTGGVEPTQENLTSWIK
CCHHHCCCHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCHHHEEEECCCCCCHHHHHHHHH
AGVFCVGMGSKLFPGDKVAAEDWAYVTAKCKEALGYIAEARANK
HCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]