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The map label for this gene is lipB [H]

Identifier: 150005542

GI number: 150005542

Start: 3832943

End: 3834031

Strand: Direct

Name: lipB [H]

Synonym: BVU_3026

Alternate gene names: 150005542

Gene position: 3832943-3834031 (Clockwise)

Preceding gene: 150005539

Following gene: 150005545

Centisome position: 74.24

GC content: 42.79

Gene sequence:

>1089_bases
ATGTATCAAATAATTCAACCTACGCCCGATGACTTTGATGAATTGACCTGTTTATGGGAAGCTTCGGTGCGTGCTACTTA
TCATTTCATACCTGAAGCATATATTCAGAAGTTAAAGCCACTGGTATGGAGTGTTTATCTTCATAGTATGCCGCTTTACA
TGATTCGTGACAATGCTGGTATAGAAGGATTCATGGGTATTAATGGGACTATGCTTGAAATGCTTTTTGTTCATCCGCGT
GCCATAGGTACGGGAATAGGGAAACAATTGATGCGTTATGCTTTGGAGCATTGCCATGTACGTTATGTCGATGTAAACGA
ACAAAATAAGAAAGCGTCCGGCTTTTATGGCCATTTTGGATTTCGGGTTATTGGCAGGGATGCAAAAGACGCATCGGGCG
AACCCTATCCTATTTTACATTTAAAACTGGGAGGTATCATGAAAATTGAAAATTGGGGATTAGTTCCCTATTCTGAGGCT
TGGAAACGGCAGACGGAGTTGTTTAATGCCGTGGTGGAAGCTAAACAAGTGGGAAAAACGTATGAGAATAGAATTATTTT
TGTAGAACATCCGCATGTCTATACATTAGGCAAGAGTGGAAAGGAAACGAATATGCTGCTGGGGGAGGCACAATTGAAGA
TGATTGGTGCCACCCTTTATCATATTGATCGAGGAGGGGATATTACTTATCATGGTCCCGGACAATTGGTTTGTTATCCT
ATCTTGAATTTGGAGGATTATCATTTGGGGTTGAAGGAATATATTCATGTGTTGGAAGAAGCGGTGATCAGGGTCTGCGC
ATCTTATGGCATTGAAGCCGGGCGAGTGAAGGGAGCGACCGGAGTATGGCTGGCCGCGGGAACTCCGCAGGAACGGAAAA
TTTGTGCAATAGGAGTGAGAAGCAGTCATTTTGTGACAATGCATGGATTGGCGCTGAATGTAAATACGGATTTGCGCTAT
TTCAGTTATATCCATCCTTGCGGATTCATGGATAAAGGGGTTACTTCATTACAGAAAGAATTGGGGTGCGAAGTGCCTAT
GGAAGAAGTGGCAGGACGTTTGCAGAACGAATTATCTGAACTTTTGTAG

Upstream 100 bases:

>100_bases
AATTCTATTGGAAAATCATTGATGTACGGCAACATATTCTTTCTTTTGATTGGGGTTGCAAATATACAATAATTATCCCA
CCTTTGCAGAAAAAAGTAAT

Downstream 100 bases:

>100_bases
TTATACCGTATGTGTGGCTTTATACATACGGGGCGGAGGGATTAAAAATCCGATTGTGTTATCATGTTGTCAACTGTAGG
GGCAGGGTTTGCCTGCCCAA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MYQIIQPTPDDFDELTCLWEASVRATYHFIPEAYIQKLKPLVWSVYLHSMPLYMIRDNAGIEGFMGINGTMLEMLFVHPR
AIGTGIGKQLMRYALEHCHVRYVDVNEQNKKASGFYGHFGFRVIGRDAKDASGEPYPILHLKLGGIMKIENWGLVPYSEA
WKRQTELFNAVVEAKQVGKTYENRIIFVEHPHVYTLGKSGKETNMLLGEAQLKMIGATLYHIDRGGDITYHGPGQLVCYP
ILNLEDYHLGLKEYIHVLEEAVIRVCASYGIEAGRVKGATGVWLAAGTPQERKICAIGVRSSHFVTMHGLALNVNTDLRY
FSYIHPCGFMDKGVTSLQKELGCEVPMEEVAGRLQNELSELL

Sequences:

>Translated_362_residues
MYQIIQPTPDDFDELTCLWEASVRATYHFIPEAYIQKLKPLVWSVYLHSMPLYMIRDNAGIEGFMGINGTMLEMLFVHPR
AIGTGIGKQLMRYALEHCHVRYVDVNEQNKKASGFYGHFGFRVIGRDAKDASGEPYPILHLKLGGIMKIENWGLVPYSEA
WKRQTELFNAVVEAKQVGKTYENRIIFVEHPHVYTLGKSGKETNMLLGEAQLKMIGATLYHIDRGGDITYHGPGQLVCYP
ILNLEDYHLGLKEYIHVLEEAVIRVCASYGIEAGRVKGATGVWLAAGTPQERKICAIGVRSSHFVTMHGLALNVNTDLRY
FSYIHPCGFMDKGVTSLQKELGCEVPMEEVAGRLQNELSELL
>Mature_362_residues
MYQIIQPTPDDFDELTCLWEASVRATYHFIPEAYIQKLKPLVWSVYLHSMPLYMIRDNAGIEGFMGINGTMLEMLFVHPR
AIGTGIGKQLMRYALEHCHVRYVDVNEQNKKASGFYGHFGFRVIGRDAKDASGEPYPILHLKLGGIMKIENWGLVPYSEA
WKRQTELFNAVVEAKQVGKTYENRIIFVEHPHVYTLGKSGKETNMLLGEAQLKMIGATLYHIDRGGDITYHGPGQLVCYP
ILNLEDYHLGLKEYIHVLEEAVIRVCASYGIEAGRVKGATGVWLAAGTPQERKICAIGVRSSHFVTMHGLALNVNTDLRY
FSYIHPCGFMDKGVTSLQKELGCEVPMEEVAGRLQNELSELL

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=213, Percent_Identity=39.4366197183099, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI87081767, Length=180, Percent_Identity=37.7777777777778, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790442, Length=134, Percent_Identity=38.0597014925373, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323268, Length=199, Percent_Identity=29.6482412060301, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355483, Length=200, Percent_Identity=39.5, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 40814; Mature: 40814

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQIIQPTPDDFDELTCLWEASVRATYHFIPEAYIQKLKPLVWSVYLHSMPLYMIRDNAG
CCEECCCCCCCHHHEEEEEEHHHHEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCC
IEGFMGINGTMLEMLFVHPRAIGTGIGKQLMRYALEHCHVRYVDVNEQNKKASGFYGHFG
CCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEECCE
FRVIGRDAKDASGEPYPILHLKLGGIMKIENWGLVPYSEAWKRQTELFNAVVEAKQVGKT
EEEEECCCCCCCCCCCEEEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCH
YENRIIFVEHPHVYTLGKSGKETNMLLGEAQLKMIGATLYHIDRGGDITYHGPGQLVCYP
HHCEEEEEECCEEEEECCCCCCCCEEECHHHHHHHEEEEEEEECCCEEEECCCCCEEEEE
ILNLEDYHLGLKEYIHVLEEAVIRVCASYGIEAGRVKGATGVWLAAGTPQERKICAIGVR
ECCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEECC
SSHFVTMHGLALNVNTDLRYFSYIHPCGFMDKGVTSLQKELGCEVPMEEVAGRLQNELSE
CCEEEEEEEEEEEECCCCHHHHEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LL
HC
>Mature Secondary Structure
MYQIIQPTPDDFDELTCLWEASVRATYHFIPEAYIQKLKPLVWSVYLHSMPLYMIRDNAG
CCEECCCCCCCHHHEEEEEEHHHHEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCC
IEGFMGINGTMLEMLFVHPRAIGTGIGKQLMRYALEHCHVRYVDVNEQNKKASGFYGHFG
CCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEECCE
FRVIGRDAKDASGEPYPILHLKLGGIMKIENWGLVPYSEAWKRQTELFNAVVEAKQVGKT
EEEEECCCCCCCCCCCEEEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCH
YENRIIFVEHPHVYTLGKSGKETNMLLGEAQLKMIGATLYHIDRGGDITYHGPGQLVCYP
HHCEEEEEECCEEEEECCCCCCCCEEECHHHHHHHEEEEEEEECCCEEEECCCCCEEEEE
ILNLEDYHLGLKEYIHVLEEAVIRVCASYGIEAGRVKGATGVWLAAGTPQERKICAIGVR
ECCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEECC
SSHFVTMHGLALNVNTDLRYFSYIHPCGFMDKGVTSLQKELGCEVPMEEVAGRLQNELSE
CCEEEEEEEEEEEECCCCHHHHEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LL
HC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]