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The map label for this gene is mscM [H]

Identifier: 150005246

GI number: 150005246

Start: 3461051

End: 3462400

Strand: Direct

Name: mscM [H]

Synonym: BVU_2718

Alternate gene names: 150005246

Gene position: 3461051-3462400 (Clockwise)

Preceding gene: 150005244

Following gene: 150005249

Centisome position: 67.03

GC content: 41.85

Gene sequence:

>1350_bases
ATGAGTACAAACGAATTTGCCCGAATTTTATTGACTTGGATTAAGAGTTTTCTATCATGGATAGGCATCCCGCGCGATAG
GTTGAATGAACTGGATGAGATTATTTTCTTGATCCTGATTGTGGTTATTGCTTTTGCTGTAGGAGCTGTTTTTCATTATT
TATCTGTGCGTTTCACACGGAAGGTGTTGAAATATAAAAATATCAGTTTTTTAAGTTCGTTGATAGAATACAATGCCTTG
CGGAAAATGTCTGCGGTCATTCCGCCGCTTATTATATCCGCTTTGTTGCCGTTTGCGTTCGACTATCGTTCTACTTGGTT
TACTGTCAGTGAAAAAATCACTTGGATTTATTTCTTTATCGCTTTGTTGTTTTCGGTCAATGCCGTGTTAAACTCGGTAG
GTAATGTGCTGATGAACAAGGAGCAGTTACAGAATCGTCCGATGAAAGGTTTCATACAGATCTTTCAAGTGATTTTTTCG
TGTGTTGCCATCATTGTCATCATTTCCATATTGATTAATAAGTCGCCTCTCAACCTGATTACCGGACTGGGTGCTTTTGC
TGCCGTACTGATGTTGATTTTCAAAGATACCATCCTTGGCTTTGTGGCGGGGGTACTGCTTTCCGAGAATGATATGGTGC
ATATCGGCGATTGGATTGAGATGCCGCAGAATAATGTGAATGGGGTGGTGATGGATATCACTCTGAACATTGTGAAGGTT
CAGAATTTCGATAATACGATTGTCACTATACCTCCTTATTCATTAGTCTCCGGTTCATTTATCAATTGGCGTGGAATGAC
TGAATCGGGCGGGCGGCGTATTATGCGCGAGTATGCATTGAAACTGGATTACATACAACCCTGTACTCCTGAATTTTTGG
AGAAAATGAAGAAGTTTGATGCTGATTTGGCCGACTTTATCACAGAAAAGCAGAAACAGGCGGCTGAAGGAAAAGTAGCG
AATACTGACAATCCGGCCGGATTGGTAAATGGAACCATCGATACGAATGTAGGGCTGCTTCGTGCTTATATGACTCTCTA
TCTGAAGCGCCATCCTTTTATCAGTAAGGACCTGTTGCTGATGGTGCGCACATTGGCTCCTACGGAGAATGGCCTGCCCG
TACAAATTTATTGTTTCTCGTCCAATAAGAACTGGCCTAGTTATGAATCTATCCAAGCGGAAATCATGGAACATTTTGTT
TCTGTTCTTCCCGAGTTTGGATTGTATCCGTTCCAGAATCCTACTGCCAGAGATTATGTAATCAGTGGTTTGATAGAGTC
TGGCAAGGACCTTTCTACTGTAGACGGAATTCCTTGGCATTCGGTCTTGCCAAAGGAAGAAAAGGTATAG

Upstream 100 bases:

>100_bases
AAGCAGGAAACCGTTTCCGGACAGGCTGGGGGAGGATTTTTCATCCCTCAGCTAAACAAATGTTTTTATAGATGGTTCTA
TAAGTAAAAAGACGATAATT

Downstream 100 bases:

>100_bases
TTAGGGATTTAAACATTTATTAATGACTTGCGGTTGAACAATTCTTGTTGCCGCCGTGTTATATAAATGTGTTTTTCATA
GTATTAGATTAAAGGTTGAA

Product: putative membrane transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSTNEFARILLTWIKSFLSWIGIPRDRLNELDEIIFLILIVVIAFAVGAVFHYLSVRFTRKVLKYKNISFLSSLIEYNAL
RKMSAVIPPLIISALLPFAFDYRSTWFTVSEKITWIYFFIALLFSVNAVLNSVGNVLMNKEQLQNRPMKGFIQIFQVIFS
CVAIIVIISILINKSPLNLITGLGAFAAVLMLIFKDTILGFVAGVLLSENDMVHIGDWIEMPQNNVNGVVMDITLNIVKV
QNFDNTIVTIPPYSLVSGSFINWRGMTESGGRRIMREYALKLDYIQPCTPEFLEKMKKFDADLADFITEKQKQAAEGKVA
NTDNPAGLVNGTIDTNVGLLRAYMTLYLKRHPFISKDLLLMVRTLAPTENGLPVQIYCFSSNKNWPSYESIQAEIMEHFV
SVLPEFGLYPFQNPTARDYVISGLIESGKDLSTVDGIPWHSVLPKEEKV

Sequences:

>Translated_449_residues
MSTNEFARILLTWIKSFLSWIGIPRDRLNELDEIIFLILIVVIAFAVGAVFHYLSVRFTRKVLKYKNISFLSSLIEYNAL
RKMSAVIPPLIISALLPFAFDYRSTWFTVSEKITWIYFFIALLFSVNAVLNSVGNVLMNKEQLQNRPMKGFIQIFQVIFS
CVAIIVIISILINKSPLNLITGLGAFAAVLMLIFKDTILGFVAGVLLSENDMVHIGDWIEMPQNNVNGVVMDITLNIVKV
QNFDNTIVTIPPYSLVSGSFINWRGMTESGGRRIMREYALKLDYIQPCTPEFLEKMKKFDADLADFITEKQKQAAEGKVA
NTDNPAGLVNGTIDTNVGLLRAYMTLYLKRHPFISKDLLLMVRTLAPTENGLPVQIYCFSSNKNWPSYESIQAEIMEHFV
SVLPEFGLYPFQNPTARDYVISGLIESGKDLSTVDGIPWHSVLPKEEKV
>Mature_448_residues
STNEFARILLTWIKSFLSWIGIPRDRLNELDEIIFLILIVVIAFAVGAVFHYLSVRFTRKVLKYKNISFLSSLIEYNALR
KMSAVIPPLIISALLPFAFDYRSTWFTVSEKITWIYFFIALLFSVNAVLNSVGNVLMNKEQLQNRPMKGFIQIFQVIFSC
VAIIVIISILINKSPLNLITGLGAFAAVLMLIFKDTILGFVAGVLLSENDMVHIGDWIEMPQNNVNGVVMDITLNIVKVQ
NFDNTIVTIPPYSLVSGSFINWRGMTESGGRRIMREYALKLDYIQPCTPEFLEKMKKFDADLADFITEKQKQAAEGKVAN
TDNPAGLVNGTIDTNVGLLRAYMTLYLKRHPFISKDLLLMVRTLAPTENGLPVQIYCFSSNKNWPSYESIQAEIMEHFVS
VLPEFGLYPFQNPTARDYVISGLIESGKDLSTVDGIPWHSVLPKEEKV

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=394, Percent_Identity=36.5482233502538, Blast_Score=257, Evalue=9e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 50766; Mature: 50635

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNEFARILLTWIKSFLSWIGIPRDRLNELDEIIFLILIVVIAFAVGAVFHYLSVRFTR
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVLKYKNISFLSSLIEYNALRKMSAVIPPLIISALLPFAFDYRSTWFTVSEKITWIYFFI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
ALLFSVNAVLNSVGNVLMNKEQLQNRPMKGFIQIFQVIFSCVAIIVIISILINKSPLNLI
HHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TGLGAFAAVLMLIFKDTILGFVAGVLLSENDMVHIGDWIEMPQNNVNGVVMDITLNIVKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHCCCCCCCEEEEEEEEEEEEE
QNFDNTIVTIPPYSLVSGSFINWRGMTESGGRRIMREYALKLDYIQPCTPEFLEKMKKFD
ECCCCEEEEECCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
ADLADFITEKQKQAAEGKVANTDNPAGLVNGTIDTNVGLLRAYMTLYLKRHPFISKDLLL
CHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
MVRTLAPTENGLPVQIYCFSSNKNWPSYESIQAEIMEHFVSVLPEFGLYPFQNPTARDYV
HHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
ISGLIESGKDLSTVDGIPWHSVLPKEEKV
HHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STNEFARILLTWIKSFLSWIGIPRDRLNELDEIIFLILIVVIAFAVGAVFHYLSVRFTR
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVLKYKNISFLSSLIEYNALRKMSAVIPPLIISALLPFAFDYRSTWFTVSEKITWIYFFI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
ALLFSVNAVLNSVGNVLMNKEQLQNRPMKGFIQIFQVIFSCVAIIVIISILINKSPLNLI
HHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TGLGAFAAVLMLIFKDTILGFVAGVLLSENDMVHIGDWIEMPQNNVNGVVMDITLNIVKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHCCCCCCCEEEEEEEEEEEEE
QNFDNTIVTIPPYSLVSGSFINWRGMTESGGRRIMREYALKLDYIQPCTPEFLEKMKKFD
ECCCCEEEEECCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
ADLADFITEKQKQAAEGKVANTDNPAGLVNGTIDTNVGLLRAYMTLYLKRHPFISKDLLL
CHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
MVRTLAPTENGLPVQIYCFSSNKNWPSYESIQAEIMEHFVSVLPEFGLYPFQNPTARDYV
HHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
ISGLIESGKDLSTVDGIPWHSVLPKEEKV
HHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]