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The map label for this gene is mdeA [H]

Identifier: 150005239

GI number: 150005239

Start: 3450633

End: 3451919

Strand: Direct

Name: mdeA [H]

Synonym: BVU_2710

Alternate gene names: 150005239

Gene position: 3450633-3451919 (Clockwise)

Preceding gene: 150005238

Following gene: 150005240

Centisome position: 66.83

GC content: 44.52

Gene sequence:

>1287_bases
ATGGCTACAAAAAAATTACATTTCGAAACATTACAACTTCACGTAGGACAGGAACAGCCAGACCCGGCTACGGATGCTCG
TGCAGTTCCTATTTATCAGACTACATCTTATGTATTTCATAACTCGGCACATGCAGCCGCACGTTTTGGTTTACAAGATC
CGGGTAATATTTATGGGCGTCTGACTAATTCCACACAAGGTGTTTTTGAACAACGTGTGGCTGCATTGGAAGGGGGAGTG
GCAGGACTTGCGGTAGCTTCCGGGGCTGCTGCCATTACTTATGCGTTTCAGAACATTACTCGTGCAGGTGATCATGTTGT
AGCTGCAAAGACTATTTACGGAGGTACTTATAACCTGCTGGCGCATACGTTGCCGACTTATGGTGTGACTGCTACATTTG
TGGACCCTGGTGATCTTTCTAATTTTGAGAAAGCTATTCAGGAGAATACAAAGGCGGTGTTTATCGAGACTTTGGGGAAT
CCTAATTCGAATATTATAGATATAGAGGCTGTGGCGGAAATAGCACATCGCCATCACATACCTTTGATTATTGATAACAC
TTTTGGTACTCCTTATCTGATTCGTCCCATCGAGCACGGAGCAGATATTGTGGTACATTCCGCTACAAAATTTATTGGTG
GCCATGGAACTTCACTGGGTGGCGTGATTGTTGATTCAGGTAAATTTGACTGGGTGGCATCAGGTAAATTCCCTCAACTG
ACAGAATCGGATCCTTGCTATCATGGGGTGCGTTTTGTAGAGGCGGCAGGTCCGGCTGCTTATGCTGTCCGTATTCGTGC
TATTTTATTGCGTGATACCGGTGCAACTATCAGTCCGTTTAATGCTTTTATTCTGTTGCAGGGATTGGAGACACTTTCAT
TGCGTGTGGAACGTCATGTAGAGAATGCGTTGAAAGTGGTGGAGCATTTGAAAAATCATCCGAAGATCAAGAAAGTAAAT
CACCCTTCCTTGCCGGAACATCCTGACCATGCTCTGTATCAGCGTTATTTCCCCAATGGAGCTAGTTCTATTTTCACTAT
CGAAGTGAAAGGTGGTCAGGAAGAAGCACATCGTTTCATCGATAGTTTGGAAATCTTTTCTTTATTGGCCAATGTCGCCG
ATGTGAAGTCATTGGTTATCCATCCTGCCAGTACCACTCACTCCCAGTTGAATGCGGAAGAATTGGCGGAACAGGGTATT
TATCCGGGGACAGTGCGTTTATCAATAGGTACGGAACATATAGATGATTTGCTGGCCGATTTGGATCAGGCATTAGCTGG
AATTTAA

Upstream 100 bases:

>100_bases
TATTTTCTTTATTTTTAGTATATCTTGTTTGATATAACTGCCTTTAATACCTTTGCAACAGGTAAAAACAGAAATATATA
ACTTTAAAAAGATAAAGATT

Downstream 100 bases:

>100_bases
TAAGATATATTAATAGAATAAGGAATATTACTACAATATAAAATCAATATATGGATAAGAAGTGTATGCCGGAGGTTTGT
GTAAAGATACATTATACATG

Product: O-acetylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MATKKLHFETLQLHVGQEQPDPATDARAVPIYQTTSYVFHNSAHAAARFGLQDPGNIYGRLTNSTQGVFEQRVAALEGGV
AGLAVASGAAAITYAFQNITRAGDHVVAAKTIYGGTYNLLAHTLPTYGVTATFVDPGDLSNFEKAIQENTKAVFIETLGN
PNSNIIDIEAVAEIAHRHHIPLIIDNTFGTPYLIRPIEHGADIVVHSATKFIGGHGTSLGGVIVDSGKFDWVASGKFPQL
TESDPCYHGVRFVEAAGPAAYAVRIRAILLRDTGATISPFNAFILLQGLETLSLRVERHVENALKVVEHLKNHPKIKKVN
HPSLPEHPDHALYQRYFPNGASSIFTIEVKGGQEEAHRFIDSLEIFSLLANVADVKSLVIHPASTTHSQLNAEELAEQGI
YPGTVRLSIGTEHIDDLLADLDQALAGI

Sequences:

>Translated_428_residues
MATKKLHFETLQLHVGQEQPDPATDARAVPIYQTTSYVFHNSAHAAARFGLQDPGNIYGRLTNSTQGVFEQRVAALEGGV
AGLAVASGAAAITYAFQNITRAGDHVVAAKTIYGGTYNLLAHTLPTYGVTATFVDPGDLSNFEKAIQENTKAVFIETLGN
PNSNIIDIEAVAEIAHRHHIPLIIDNTFGTPYLIRPIEHGADIVVHSATKFIGGHGTSLGGVIVDSGKFDWVASGKFPQL
TESDPCYHGVRFVEAAGPAAYAVRIRAILLRDTGATISPFNAFILLQGLETLSLRVERHVENALKVVEHLKNHPKIKKVN
HPSLPEHPDHALYQRYFPNGASSIFTIEVKGGQEEAHRFIDSLEIFSLLANVADVKSLVIHPASTTHSQLNAEELAEQGI
YPGTVRLSIGTEHIDDLLADLDQALAGI
>Mature_427_residues
ATKKLHFETLQLHVGQEQPDPATDARAVPIYQTTSYVFHNSAHAAARFGLQDPGNIYGRLTNSTQGVFEQRVAALEGGVA
GLAVASGAAAITYAFQNITRAGDHVVAAKTIYGGTYNLLAHTLPTYGVTATFVDPGDLSNFEKAIQENTKAVFIETLGNP
NSNIIDIEAVAEIAHRHHIPLIIDNTFGTPYLIRPIEHGADIVVHSATKFIGGHGTSLGGVIVDSGKFDWVASGKFPQLT
ESDPCYHGVRFVEAAGPAAYAVRIRAILLRDTGATISPFNAFILLQGLETLSLRVERHVENALKVVEHLKNHPKIKKVNH
PSLPEHPDHALYQRYFPNGASSIFTIEVKGGQEEAHRFIDSLEIFSLLANVADVKSLVIHPASTTHSQLNAEELAEQGIY
PGTVRLSIGTEHIDDLLADLDQALAGI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=420, Percent_Identity=33.8095238095238, Blast_Score=212, Evalue=7e-55,
Organism=Homo sapiens, GI299473759, Length=420, Percent_Identity=31.1904761904762, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI25453487, Length=419, Percent_Identity=28.6396181384248, Blast_Score=149, Evalue=7e-36,
Organism=Escherichia coli, GI1790375, Length=397, Percent_Identity=30.2267002518892, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1789383, Length=437, Percent_Identity=28.604118993135, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71980708, Length=432, Percent_Identity=32.1759259259259, Blast_Score=219, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=34.51536643026, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17560056, Length=422, Percent_Identity=34.8341232227488, Blast_Score=207, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=48.2758620689655, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319307, Length=374, Percent_Identity=33.4224598930481, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6321170, Length=365, Percent_Identity=26.027397260274, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6321254, Length=427, Percent_Identity=25.0585480093677, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321904, Length=294, Percent_Identity=28.2312925170068, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=36.4066193853428, Blast_Score=241, Evalue=6e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46213; Mature: 46081

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKKLHFETLQLHVGQEQPDPATDARAVPIYQTTSYVFHNSAHAAARFGLQDPGNIYGR
CCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCEEEEECCCHHHHHCCCCCCCCEEEE
LTNSTQGVFEQRVAALEGGVAGLAVASGAAAITYAFQNITRAGDHVVAAKTIYGGTYNLL
ECCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEEECCHHHHH
AHTLPTYGVTATFVDPGDLSNFEKAIQENTKAVFIETLGNPNSNIIDIEAVAEIAHRHHI
HHHCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHHHCCC
PLIIDNTFGTPYLIRPIEHGADIVVHSATKFIGGHGTSLGGVIVDSGKFDWVASGKFPQL
CEEEECCCCCCEEEEECCCCCCEEEEEHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCC
TESDPCYHGVRFVEAAGPAAYAVRIRAILLRDTGATISPFNAFILLQGLETLSLRVERHV
CCCCCCHHCEEEEECCCCHHEEEEEEEEEEECCCCCCCCHHHHHEECCHHHHHHHHHHHH
ENALKVVEHLKNHPKIKKVNHPSLPEHPDHALYQRYFPNGASSIFTIEVKGGQEEAHRFI
HHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHH
DSLEIFSLLANVADVKSLVIHPASTTHSQLNAEELAEQGIYPGTVRLSIGTEHIDDLLAD
HHHHHHHHHHHHHHHHHHEEECCCCCHHHCCHHHHHHCCCCCCEEEEEECHHHHHHHHHH
LDQALAGI
HHHHHHCC
>Mature Secondary Structure 
ATKKLHFETLQLHVGQEQPDPATDARAVPIYQTTSYVFHNSAHAAARFGLQDPGNIYGR
CCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCEEEEECCCHHHHHCCCCCCCCEEEE
LTNSTQGVFEQRVAALEGGVAGLAVASGAAAITYAFQNITRAGDHVVAAKTIYGGTYNLL
ECCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEEECCHHHHH
AHTLPTYGVTATFVDPGDLSNFEKAIQENTKAVFIETLGNPNSNIIDIEAVAEIAHRHHI
HHHCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHHHCCC
PLIIDNTFGTPYLIRPIEHGADIVVHSATKFIGGHGTSLGGVIVDSGKFDWVASGKFPQL
CEEEECCCCCCEEEEECCCCCCEEEEEHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCC
TESDPCYHGVRFVEAAGPAAYAVRIRAILLRDTGATISPFNAFILLQGLETLSLRVERHV
CCCCCCHHCEEEEECCCCHHEEEEEEEEEEECCCCCCCCHHHHHEECCHHHHHHHHHHHH
ENALKVVEHLKNHPKIKKVNHPSLPEHPDHALYQRYFPNGASSIFTIEVKGGQEEAHRFI
HHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHH
DSLEIFSLLANVADVKSLVIHPASTTHSQLNAEELAEQGIYPGTVRLSIGTEHIDDLLAD
HHHHHHHHHHHHHHHHHHEEECCCCCHHHCCHHHHHHCCCCCCEEEEEECHHHHHHHHHH
LDQALAGI
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]