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The map label for this gene is pit [H]

Identifier: 150005235

GI number: 150005235

Start: 3446158

End: 3447171

Strand: Direct

Name: pit [H]

Synonym: BVU_2706

Alternate gene names: 150005235

Gene position: 3446158-3447171 (Clockwise)

Preceding gene: 150005234

Following gene: 150005236

Centisome position: 66.74

GC content: 41.52

Gene sequence:

>1014_bases
ATGGAATTATTAGTGATTATTATTACTTTGGCTTTGATTTTTGACTATATTAACGGATTTCATGATGCAGCCAATTCAAT
AGCCACCATTGTGTCAACCAAGGTTCTTACTCCGTTCCAAGCGGTGATATGGGCGGCATTTTTTAATTTTGTTGCATTCT
TTATTGCTAAATGTATAATCGGAGGGTTCGGAATAGCCAATACTGTATCCAAGACAGTAGTGGAACAGTATATTTCCTTG
CCGATTATTCTTTCGGGGGTTATTGCAGCTATTGTCTGGAATCTGTTTACATGGTGGAAGGGGATTCCTTCTTCTTCTTC
GCATACGCTGATCGGAGGTTTTGCCGGTGCTGCTATTATGGCTCATGGTTTTGATGCCATTCAGCTCAATATTATTTTGA
AGATTGCTGTTTTTATCTTTTTGGCACCATTTATAGGGATGGTTGTTGCTTTTGGATTTACGTTGCTTGTCCTTCACATT
TGTCGTCGTGCACATCCGAATACGGCTGAGATGTGGTTTAAGAAATTGCAGCTTGTTTCTTCGGCTTTGTTCAGTATAGG
GCATGGACTGAATGATTCCCAGAAGGTAATGGGTATTATTGCTGCTGCCCTGATCGCTGCTCATTCGGAAGGGTTAAGAA
TGGGAATAAATAGTATCAATGATTTGCCGGATTGGGTGGCATTTTCTTGTTTTACTGCCATTTCTTTAGGAACGATGTCA
GGCGGATGGAAGATTGTGAAAACCATGGGAACTAAGATTACCAAAGTAACTCCTTTGGAGGGAGTTGTTGCGGAAACTGC
TGGCGCATTTACATTGTATCTGACAGAATATTTGAAAATACCTGTAAGTACAACCCACACCATTACAGGAGCTATTATCG
GAGTGGGTGCTACTAAGCGTCTTTCAGCCGTCCGATGGGGAATAACAAGAAGCCTGATGACGGCTTGGGTGTTGACTATT
CCTGTCAGTGGAATATTGGCCGCTGTTATTTACGGAGTGGTTACTCTTTTTTAG

Upstream 100 bases:

>100_bases
ATAATGCATGAATTGGAGAAAACGACCGATGCGGCAGAACGGGTAGGAAAAATCTTGAGAAACCTGATTGTGAAATACGC
ATAAAAGCAGAAGGTACAAT

Downstream 100 bases:

>100_bases
AATGACCGGATTGTAGGCTGTTCTAGAAAATAAAGATCTGTCGTGACCGAGGTGACTGGGAAGTTACCTCGGTTTTGTAT
ACATACCCATTTGTAGGTAA

Product: putative inorganic phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MELLVIIITLALIFDYINGFHDAANSIATIVSTKVLTPFQAVIWAAFFNFVAFFIAKCIIGGFGIANTVSKTVVEQYISL
PIILSGVIAAIVWNLFTWWKGIPSSSSHTLIGGFAGAAIMAHGFDAIQLNIILKIAVFIFLAPFIGMVVAFGFTLLVLHI
CRRAHPNTAEMWFKKLQLVSSALFSIGHGLNDSQKVMGIIAAALIAAHSEGLRMGINSINDLPDWVAFSCFTAISLGTMS
GGWKIVKTMGTKITKVTPLEGVVAETAGAFTLYLTEYLKIPVSTTHTITGAIIGVGATKRLSAVRWGITRSLMTAWVLTI
PVSGILAAVIYGVVTLF

Sequences:

>Translated_337_residues
MELLVIIITLALIFDYINGFHDAANSIATIVSTKVLTPFQAVIWAAFFNFVAFFIAKCIIGGFGIANTVSKTVVEQYISL
PIILSGVIAAIVWNLFTWWKGIPSSSSHTLIGGFAGAAIMAHGFDAIQLNIILKIAVFIFLAPFIGMVVAFGFTLLVLHI
CRRAHPNTAEMWFKKLQLVSSALFSIGHGLNDSQKVMGIIAAALIAAHSEGLRMGINSINDLPDWVAFSCFTAISLGTMS
GGWKIVKTMGTKITKVTPLEGVVAETAGAFTLYLTEYLKIPVSTTHTITGAIIGVGATKRLSAVRWGITRSLMTAWVLTI
PVSGILAAVIYGVVTLF
>Mature_337_residues
MELLVIIITLALIFDYINGFHDAANSIATIVSTKVLTPFQAVIWAAFFNFVAFFIAKCIIGGFGIANTVSKTVVEQYISL
PIILSGVIAAIVWNLFTWWKGIPSSSSHTLIGGFAGAAIMAHGFDAIQLNIILKIAVFIFLAPFIGMVVAFGFTLLVLHI
CRRAHPNTAEMWFKKLQLVSSALFSIGHGLNDSQKVMGIIAAALIAAHSEGLRMGINSINDLPDWVAFSCFTAISLGTMS
GGWKIVKTMGTKITKVTPLEGVVAETAGAFTLYLTEYLKIPVSTTHTITGAIIGVGATKRLSAVRWGITRSLMTAWVLTI
PVSGILAAVIYGVVTLF

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=158, Percent_Identity=32.2784810126582, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI31543630, Length=183, Percent_Identity=27.8688524590164, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1789360, Length=239, Percent_Identity=30.1255230125523, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789907, Length=235, Percent_Identity=31.4893617021277, Blast_Score=98, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI25146401, Length=166, Percent_Identity=27.710843373494, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=28.3950617283951, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17539280, Length=178, Percent_Identity=26.4044943820225, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=170, Percent_Identity=27.6470588235294, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32566716, Length=168, Percent_Identity=29.7619047619048, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319773, Length=173, Percent_Identity=29.4797687861272, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 36112; Mature: 36112

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLVIIITLALIFDYINGFHDAANSIATIVSTKVLTPFQAVIWAAFFNFVAFFIAKCII
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GGFGIANTVSKTVVEQYISLPIILSGVIAAIVWNLFTWWKGIPSSSSHTLIGGFAGAAIM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHH
AHGFDAIQLNIILKIAVFIFLAPFIGMVVAFGFTLLVLHICRRAHPNTAEMWFKKLQLVS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SALFSIGHGLNDSQKVMGIIAAALIAAHSEGLRMGINSINDLPDWVAFSCFTAISLGTMS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
GGWKIVKTMGTKITKVTPLEGVVAETAGAFTLYLTEYLKIPVSTTHTITGAIIGVGATKR
CCHHHHHHHCCCCEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHEECCCHHH
LSAVRWGITRSLMTAWVLTIPVSGILAAVIYGVVTLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELLVIIITLALIFDYINGFHDAANSIATIVSTKVLTPFQAVIWAAFFNFVAFFIAKCII
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GGFGIANTVSKTVVEQYISLPIILSGVIAAIVWNLFTWWKGIPSSSSHTLIGGFAGAAIM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHH
AHGFDAIQLNIILKIAVFIFLAPFIGMVVAFGFTLLVLHICRRAHPNTAEMWFKKLQLVS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SALFSIGHGLNDSQKVMGIIAAALIAAHSEGLRMGINSINDLPDWVAFSCFTAISLGTMS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
GGWKIVKTMGTKITKVTPLEGVVAETAGAFTLYLTEYLKIPVSTTHTITGAIIGVGATKR
CCHHHHHHHCCCCEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHEECCCHHH
LSAVRWGITRSLMTAWVLTIPVSGILAAVIYGVVTLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]