Click here to switch to the map view.

The map label for this gene is eno

Identifier: 150005171

GI number: 150005171

Start: 3350886

End: 3352181

Strand: Reverse

Name: eno

Synonym: BVU_2641

Alternate gene names: 150005171

Gene position: 3352181-3350886 (Counterclockwise)

Preceding gene: 150005179

Following gene: 150005167

Centisome position: 64.92

GC content: 48.38

Gene sequence:

>1296_bases
ATGAAAATAGCAGAGATTAAAGGAAGAGAGATTTTGGATTCACGTGGTAACCCCACTGTGGAAGTGGATGTGATATTGGA
ATCAGGGATTATGGGCCGTGCCTCTGTCCCATCGGGTGCGTCAACCGGTGAACATGAGGCATTGGAGCTACGTGACGGTG
ACAAGACCCGTTACGGGGGCAAAGGCGTATTAAAAGCTGTAGAAAATATAAATACCCTTATCGCTCCGGCATTGAAAGGA
ATGGACTCTATGGACCAACGTGGCATTGACAAAGCCATGCTTGATCTGGATGGAACCCCAACTAAATCAAAACTGGGCGC
AAATGCTATTTTGGGCGTATCCCTGGCTGTGGCAAAAGCTGCCGCCAACTATCTGGATATCCCTCTTTACAGATACATCG
GCGGAACAAATACCTATGTCATGCCCGTCCCGATGATGAACATCATTAACGGAGGCTCGCATAGCGACGCTCCTATCGCT
TTTCAAGAATTCATGATTCGTCCTGTCGGCGCAAAGACATTCCGTGAGGCCTTACGGATGGGCGCAGAAGTATTCCATGC
TTTGAAAGAGGTGCTGAAAAAACGCGGACTAAGCACAGCCGTAGGAGATGAAGGCGGTTTTGCCCCTGCACTGGATGGCA
CCGAAGATGCTTTGAATTGTATCCTTGCCGCTATTGAAGCTGCCGGATATGAACCGTTCAAACATATCACCATCGGTCTG
GATTGTGCTTCTTCCGAATTTTATCATGACGGCATCTATGACTATACCAAGTTTGAAGGCCCCAAAGGGGAAAAGCGCAG
TGCCGCCGAACAGGTTGCCTATCTGGAAAAATTGAGTTGGGACTACCCCATTGATTCGATAGAGGATGGTATGGCAGAAA
ACGACTGGGAAGGATGGCGCATGCTGACCGAGCGTCTGGGCAACCGTTGCCAGCTGGTAGGTGATGACTTGTTCGTTACC
AATGTGAAGTTCCTGGAAAAAGGAATCAGTGAAGGTTGTGCAAACTCTATCCTGATAAAAGTAAACCAGATCGGTTCGCT
GACTGAAACTCTGGATGCTATCGAAATGGCCCAACGTAACGGCTACACCACTGTGACCAGCCACCGCTCCGGTGAAACGG
AAGATGCTACCATTGCAGATATCGCCGTAGCCACCAATAGCGGACAGATTAAAACCGGTTCACTGAGCCGTAGCGACCGT
ATGGCAAAATACAACCAGTTGCTCCGCATTGAAGAAGAATTGGGTAACCGTGCCGTATATGGATATAAAAAAATAGCACG
CAATTTTAAAGCGTAA

Upstream 100 bases:

>100_bases
TAAAAAAGTAAATATCATGAAAAAACTTTCCCTGGCACCTCAACAAATCCGGCTTCTCTATTGTTATTAAAATAAAACCA
TCTTATAAAATTAGAATTAT

Downstream 100 bases:

>100_bases
GCGGTTTATATCCCCAATTCCATAAATGAAGACTGCCTCAAGAGGGATCGTGATGATTCCGGCTTGAGGCATTACTTTTG
CACTATTATTTAATCTCAAC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKIAEIKGREILDSRGNPTVEVDVILESGIMGRASVPSGASTGEHEALELRDGDKTRYGGKGVLKAVENINTLIAPALKG
MDSMDQRGIDKAMLDLDGTPTKSKLGANAILGVSLAVAKAAANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIA
FQEFMIRPVGAKTFREALRMGAEVFHALKEVLKKRGLSTAVGDEGGFAPALDGTEDALNCILAAIEAAGYEPFKHITIGL
DCASSEFYHDGIYDYTKFEGPKGEKRSAAEQVAYLEKLSWDYPIDSIEDGMAENDWEGWRMLTERLGNRCQLVGDDLFVT
NVKFLEKGISEGCANSILIKVNQIGSLTETLDAIEMAQRNGYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDR
MAKYNQLLRIEEELGNRAVYGYKKIARNFKA

Sequences:

>Translated_431_residues
MKIAEIKGREILDSRGNPTVEVDVILESGIMGRASVPSGASTGEHEALELRDGDKTRYGGKGVLKAVENINTLIAPALKG
MDSMDQRGIDKAMLDLDGTPTKSKLGANAILGVSLAVAKAAANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIA
FQEFMIRPVGAKTFREALRMGAEVFHALKEVLKKRGLSTAVGDEGGFAPALDGTEDALNCILAAIEAAGYEPFKHITIGL
DCASSEFYHDGIYDYTKFEGPKGEKRSAAEQVAYLEKLSWDYPIDSIEDGMAENDWEGWRMLTERLGNRCQLVGDDLFVT
NVKFLEKGISEGCANSILIKVNQIGSLTETLDAIEMAQRNGYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDR
MAKYNQLLRIEEELGNRAVYGYKKIARNFKA
>Mature_431_residues
MKIAEIKGREILDSRGNPTVEVDVILESGIMGRASVPSGASTGEHEALELRDGDKTRYGGKGVLKAVENINTLIAPALKG
MDSMDQRGIDKAMLDLDGTPTKSKLGANAILGVSLAVAKAAANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIA
FQEFMIRPVGAKTFREALRMGAEVFHALKEVLKKRGLSTAVGDEGGFAPALDGTEDALNCILAAIEAAGYEPFKHITIGL
DCASSEFYHDGIYDYTKFEGPKGEKRSAAEQVAYLEKLSWDYPIDSIEDGMAENDWEGWRMLTERLGNRCQLVGDDLFVT
NVKFLEKGISEGCANSILIKVNQIGSLTETLDAIEMAQRNGYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDR
MAKYNQLLRIEEELGNRAVYGYKKIARNFKA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=439, Percent_Identity=53.3029612756264, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=439, Percent_Identity=53.7585421412301, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=440, Percent_Identity=53.4090909090909, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=440, Percent_Identity=53.4090909090909, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI301897479, Length=438, Percent_Identity=47.945205479452, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI169201331, Length=348, Percent_Identity=28.1609195402299, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI169201757, Length=348, Percent_Identity=28.1609195402299, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI239744207, Length=348, Percent_Identity=28.1609195402299, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI310129182, Length=135, Percent_Identity=31.8518518518519, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI310110045, Length=135, Percent_Identity=31.8518518518519, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI310120572, Length=135, Percent_Identity=31.8518518518519, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1789141, Length=427, Percent_Identity=62.5292740046838, Blast_Score=505, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17536383, Length=440, Percent_Identity=54.0909090909091, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71995829, Length=440, Percent_Identity=54.0909090909091, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32563855, Length=305, Percent_Identity=40.655737704918, Blast_Score=176, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=52.0833333333333, Blast_Score=413, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=50.2314814814815, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=50, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=50, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=50.462962962963, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=425, Percent_Identity=53.4117647058823, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=425, Percent_Identity=53.4117647058823, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=425, Percent_Identity=53.4117647058823, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=425, Percent_Identity=53.4117647058823, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=425, Percent_Identity=53.4117647058823, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=425, Percent_Identity=53.4117647058823, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_BACV8 (A6L3M9)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299915.1
- ProteinModelPortal:   A6L3M9
- SMR:   A6L3M9
- STRING:   A6L3M9
- GeneID:   5303604
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_2641
- NMPDR:   fig|435590.6.peg.2478
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46754; Mature: 46754

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 340-340 BINDING 154-154 BINDING 163-163 BINDING 288-288 BINDING 315-315 BINDING 340-340 BINDING 391-391

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAEIKGREILDSRGNPTVEVDVILESGIMGRASVPSGASTGEHEALELRDGDKTRYGG
CCCCCCCCCHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
KGVLKAVENINTLIAPALKGMDSMDQRGIDKAMLDLDGTPTKSKLGANAILGVSLAVAKA
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHH
AANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIAFQEFMIRPVGAKTFREALRM
HHHHHCCCHHHHCCCCCCEEECCCHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
GAEVFHALKEVLKKRGLSTAVGDEGGFAPALDGTEDALNCILAAIEAAGYEPFKHITIGL
HHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEE
DCASSEFYHDGIYDYTKFEGPKGEKRSAAEQVAYLEKLSWDYPIDSIEDGMAENDWEGWR
EECCCCHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHH
MLTERLGNRCQLVGDDLFVTNVKFLEKGISEGCANSILIKVNQIGSLTETLDAIEMAQRN
HHHHHHCCEEEEECCCEEEHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHC
GYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDRMAKYNQLLRIEEELGNRAVY
CCEEEECCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCHH
GYKKIARNFKA
HHHHHHHHCCC
>Mature Secondary Structure
MKIAEIKGREILDSRGNPTVEVDVILESGIMGRASVPSGASTGEHEALELRDGDKTRYGG
CCCCCCCCCHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
KGVLKAVENINTLIAPALKGMDSMDQRGIDKAMLDLDGTPTKSKLGANAILGVSLAVAKA
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHH
AANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIAFQEFMIRPVGAKTFREALRM
HHHHHCCCHHHHCCCCCCEEECCCHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
GAEVFHALKEVLKKRGLSTAVGDEGGFAPALDGTEDALNCILAAIEAAGYEPFKHITIGL
HHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEE
DCASSEFYHDGIYDYTKFEGPKGEKRSAAEQVAYLEKLSWDYPIDSIEDGMAENDWEGWR
EECCCCHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHH
MLTERLGNRCQLVGDDLFVTNVKFLEKGISEGCANSILIKVNQIGSLTETLDAIEMAQRN
HHHHHHCCEEEEECCCEEEHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHC
GYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDRMAKYNQLLRIEEELGNRAVY
CCEEEECCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCHH
GYKKIARNFKA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA