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The map label for this gene is recF

Identifier: 150005151

GI number: 150005151

Start: 3330609

End: 3331724

Strand: Direct

Name: recF

Synonym: BVU_2620

Alternate gene names: 150005151

Gene position: 3330609-3331724 (Clockwise)

Preceding gene: 150005147

Following gene: 150005152

Centisome position: 64.51

GC content: 40.5

Gene sequence:

>1116_bases
ATGATACTGAAACGCATCTCGATATTGAATTATAAAAACCTGGAGCAGGCGGAATTGGAATTCTCGCCGAAGATGAATTG
TTTTATCGGACAAAACGGTATGGGGAAGACAAATTTGCTGGATGCGGTTTATTATCTTTCTTTTTGTAAGAGTGCCACTA
ATCCTATTGATTCGCAAAATATCCGTCATGAAGGAGAATTCTTTGTAATTCAGGGTTTCTATGAAACAGACCAAGGTGAG
CCGGAAGAGGTATATTGTGGATTGAAACGTCGTCAGAAAAAGCAATTCAAGCGGAATAAAAAGGAATATAACCGTTTATC
GGATCATATTGGCTTTATTCCCCTTGTAATGGTGTCTCCTGCTGATGCGGAGTTGATTGCAGGAGGTAGTGACGGGCGCC
GTCGTTTTATGGATGTGGTGATATCGCAATATGATAAGGAATATTTGGATGCGCTGATACGTTATAATAAAGCATTGACG
CAGCGTAATGCTTTGTTGAAATCGGAACAGGAATTCGATGAGGAATTAATGCTGGTATGGGAGGAGATGATGGCATCTGC
GGGTGAGGTCGTCTTTAAGAAGCGTAGTGAGTTTATTGCTGAGTTTATACCTACTTTCCAGTCTTTTTATTCTTATATCT
CTCAAGATAAAGAGAAAGTGAATCTGGCGTATGAATCTCATGCTATGAGTGGGGGATTGCTGGATATTATCAAGGAGAGC
CGTCGGCGCGACCGTGTAATGGGATATTCGTTGAAGGGAGTTCATAAAGATGACTTGATTATGCAGTTGGGGGATTTTCC
GATAAAGCGGGAGGGGTCACAAGGACAAAACAAAACTTATCTTATCGCCTTGAAATTAGCTCAGTTTGACTTTTTGAAGA
AAACGGGCGGCAATACTACTCCTTTATTGTTGTTGGATGATATTTTTGATAAACTGGATGCTTTTCGTGTGGAACAGATT
GTAAAACTGGTAGCCGGAGATCGTTTCGGGCAGATTTTCATTACGGATACCAATCGGGATCATTTGGATAAAATATTGAA
GAAGATAGAAAGAGAATACAAAGTCTTTGCTGTAGAAGACGGAGAAGTGACAGAAAGAAAGGAAATGGCTGAATGA

Upstream 100 bases:

>100_bases
AGTAGTTCATTTCTATGTATTAATGAAATTTACGGGCATATTTTTTTAGTTTTTCCCGTTTTTATCTCTAATTTTGTATC
CTCAATTAAATAGAAGTTCT

Downstream 100 bases:

>100_bases
AACGTAATGATGCGGAACAAATAGGGGAAATGATTCGGAAATTCTTCCGGCAGAATGCTTTGGAGGCGCCACTGAACGAG
TATCGTCTGATACAGGCATG

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MILKRISILNYKNLEQAELEFSPKMNCFIGQNGMGKTNLLDAVYYLSFCKSATNPIDSQNIRHEGEFFVIQGFYETDQGE
PEEVYCGLKRRQKKQFKRNKKEYNRLSDHIGFIPLVMVSPADAELIAGGSDGRRRFMDVVISQYDKEYLDALIRYNKALT
QRNALLKSEQEFDEELMLVWEEMMASAGEVVFKKRSEFIAEFIPTFQSFYSYISQDKEKVNLAYESHAMSGGLLDIIKES
RRRDRVMGYSLKGVHKDDLIMQLGDFPIKREGSQGQNKTYLIALKLAQFDFLKKTGGNTTPLLLLDDIFDKLDAFRVEQI
VKLVAGDRFGQIFITDTNRDHLDKILKKIEREYKVFAVEDGEVTERKEMAE

Sequences:

>Translated_371_residues
MILKRISILNYKNLEQAELEFSPKMNCFIGQNGMGKTNLLDAVYYLSFCKSATNPIDSQNIRHEGEFFVIQGFYETDQGE
PEEVYCGLKRRQKKQFKRNKKEYNRLSDHIGFIPLVMVSPADAELIAGGSDGRRRFMDVVISQYDKEYLDALIRYNKALT
QRNALLKSEQEFDEELMLVWEEMMASAGEVVFKKRSEFIAEFIPTFQSFYSYISQDKEKVNLAYESHAMSGGLLDIIKES
RRRDRVMGYSLKGVHKDDLIMQLGDFPIKREGSQGQNKTYLIALKLAQFDFLKKTGGNTTPLLLLDDIFDKLDAFRVEQI
VKLVAGDRFGQIFITDTNRDHLDKILKKIEREYKVFAVEDGEVTERKEMAE
>Mature_371_residues
MILKRISILNYKNLEQAELEFSPKMNCFIGQNGMGKTNLLDAVYYLSFCKSATNPIDSQNIRHEGEFFVIQGFYETDQGE
PEEVYCGLKRRQKKQFKRNKKEYNRLSDHIGFIPLVMVSPADAELIAGGSDGRRRFMDVVISQYDKEYLDALIRYNKALT
QRNALLKSEQEFDEELMLVWEEMMASAGEVVFKKRSEFIAEFIPTFQSFYSYISQDKEKVNLAYESHAMSGGLLDIIKES
RRRDRVMGYSLKGVHKDDLIMQLGDFPIKREGSQGQNKTYLIALKLAQFDFLKKTGGNTTPLLLLDDIFDKLDAFRVEQI
VKLVAGDRFGQIFITDTNRDHLDKILKKIEREYKVFAVEDGEVTERKEMAE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=378, Percent_Identity=26.1904761904762, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_BACV8 (A6L3K9)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299895.1
- STRING:   A6L3K9
- GeneID:   5303583
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_2620
- NMPDR:   fig|435590.6.peg.2459
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK823856
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42966; Mature: 42966

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKRISILNYKNLEQAELEFSPKMNCFIGQNGMGKTNLLDAVYYLSFCKSATNPIDSQN
CCCHHHHHHCCCCCCHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
IRHEGEFFVIQGFYETDQGEPEEVYCGLKRRQKKQFKRNKKEYNRLSDHIGFIPLVMVSP
CCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
ADAELIAGGSDGRRRFMDVVISQYDKEYLDALIRYNKALTQRNALLKSEQEFDEELMLVW
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEMMASAGEVVFKKRSEFIAEFIPTFQSFYSYISQDKEKVNLAYESHAMSGGLLDIIKES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHH
RRRDRVMGYSLKGVHKDDLIMQLGDFPIKREGSQGQNKTYLIALKLAQFDFLKKTGGNTT
HHHHHHCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHCCCCCC
PLLLLDDIFDKLDAFRVEQIVKLVAGDRFGQIFITDTNRDHLDKILKKIEREYKVFAVED
CEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCEEEEECC
GEVTERKEMAE
CCHHHHHHCCC
>Mature Secondary Structure
MILKRISILNYKNLEQAELEFSPKMNCFIGQNGMGKTNLLDAVYYLSFCKSATNPIDSQN
CCCHHHHHHCCCCCCHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
IRHEGEFFVIQGFYETDQGEPEEVYCGLKRRQKKQFKRNKKEYNRLSDHIGFIPLVMVSP
CCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
ADAELIAGGSDGRRRFMDVVISQYDKEYLDALIRYNKALTQRNALLKSEQEFDEELMLVW
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEMMASAGEVVFKKRSEFIAEFIPTFQSFYSYISQDKEKVNLAYESHAMSGGLLDIIKES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHH
RRRDRVMGYSLKGVHKDDLIMQLGDFPIKREGSQGQNKTYLIALKLAQFDFLKKTGGNTT
HHHHHHCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHCCCCCC
PLLLLDDIFDKLDAFRVEQIVKLVAGDRFGQIFITDTNRDHLDKILKKIEREYKVFAVED
CEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCEEEEECC
GEVTERKEMAE
CCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA