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The map label for this gene is exoA [H]

Identifier: 150005146

GI number: 150005146

Start: 3326631

End: 3327389

Strand: Direct

Name: exoA [H]

Synonym: BVU_2613

Alternate gene names: 150005146

Gene position: 3326631-3327389 (Clockwise)

Preceding gene: 150005145

Following gene: 150005147

Centisome position: 64.43

GC content: 39.66

Gene sequence:

>759_bases
ATGTTGAAATTCATATCATGGAATGTAAATGGCTTGCGAGCCTGTTATGATAAAGGTTTTGTTGATGTTTTCCATCGTTT
AGAAGCAGACTTTTTCTGCCTGCAGGAAACAAAGATGCAGGAAGGGCAACTGGATGCCAAGTTTGAAGGTTATCACTCCT
ATTGGAATTATGCTGAAAAGAAAGGGTATTCAGGCACAGCTATCTTTTCTAAAGTCAAACCATTATCTGTGACATACGGT
TTAGGCATAGAAGAGCATGATCACGAAGGACGGGTAATCACTCTTGAATTGGAATCGTTTTATTTGATTACTGTGTATAC
TCCCAATTCGCAGGAAGAATTGCGACGGTTGGATTATAGAATGAAATGGGAAGATGATTTCCGTGCCTATCTGAAAAAAC
TGGAAGAAAAGAAACCTGTCATTGTGTGTGGTGACCTGAACGTGGCGCATAAGGAAATAGACTTGAAGAATCCTAAGACA
AACCGTAAAAATGCAGGTTTCACTGATGAGGAACGTGCCAAGTTTACCACTTTGCTTGAGTCCGGTTTTACGGATACGTT
CCGCTATTTTTATCCAGAGCAGGAAGGAATCTATTCATGGTGGTCTTATCGTTTTAAAGCCCGTGAAAAGAATTCAGGTT
GGCGTATTGATTATTTTCTCACTTCCGATTCACTGAAAGATAGGTTGAAAGGTGCACATATTTATACGGATATCTTTGGC
TCAGATCATTGTCCGGTAGAATTGACAATAGAACTCTAA

Upstream 100 bases:

>100_bases
TAGGACGCTTTTTTGTTTTAAGTTTTTTTGCTTTGATAAGTATGCTTTTGTACTTACTTTTGTTCTGGAATGTTTTTAAT
CTACAAATAATAAAAGAATC

Downstream 100 bases:

>100_bases
GAAACACTCTGAAAAAGCTTTTGTTTCAACACGAATCTCAGATTATCTATAATTAAAAGATAATCTGAGGTTTGTGTCGT
TGAAAGACTCTCTCTGTTTT

Product: exodeoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLKFISWNVNGLRACYDKGFVDVFHRLEADFFCLQETKMQEGQLDAKFEGYHSYWNYAEKKGYSGTAIFSKVKPLSVTYG
LGIEEHDHEGRVITLELESFYLITVYTPNSQEELRRLDYRMKWEDDFRAYLKKLEEKKPVIVCGDLNVAHKEIDLKNPKT
NRKNAGFTDEERAKFTTLLESGFTDTFRYFYPEQEGIYSWWSYRFKAREKNSGWRIDYFLTSDSLKDRLKGAHIYTDIFG
SDHCPVELTIEL

Sequences:

>Translated_252_residues
MLKFISWNVNGLRACYDKGFVDVFHRLEADFFCLQETKMQEGQLDAKFEGYHSYWNYAEKKGYSGTAIFSKVKPLSVTYG
LGIEEHDHEGRVITLELESFYLITVYTPNSQEELRRLDYRMKWEDDFRAYLKKLEEKKPVIVCGDLNVAHKEIDLKNPKT
NRKNAGFTDEERAKFTTLLESGFTDTFRYFYPEQEGIYSWWSYRFKAREKNSGWRIDYFLTSDSLKDRLKGAHIYTDIFG
SDHCPVELTIEL
>Mature_252_residues
MLKFISWNVNGLRACYDKGFVDVFHRLEADFFCLQETKMQEGQLDAKFEGYHSYWNYAEKKGYSGTAIFSKVKPLSVTYG
LGIEEHDHEGRVITLELESFYLITVYTPNSQEELRRLDYRMKWEDDFRAYLKKLEEKKPVIVCGDLNVAHKEIDLKNPKT
NRKNAGFTDEERAKFTTLLESGFTDTFRYFYPEQEGIYSWWSYRFKAREKNSGWRIDYFLTSDSLKDRLKGAHIYTDIFG
SDHCPVELTIEL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=257, Percent_Identity=49.0272373540856, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI18375503, Length=257, Percent_Identity=49.0272373540856, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI18375501, Length=257, Percent_Identity=49.0272373540856, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI18375507, Length=313, Percent_Identity=28.4345047923323, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=29.6992481203008, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71989536, Length=256, Percent_Identity=44.53125, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI71989539, Length=144, Percent_Identity=39.5833333333333, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI221330655, Length=250, Percent_Identity=50.8, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI17136678, Length=250, Percent_Identity=50.8, Blast_Score=264, Evalue=4e-71,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29808; Mature: 29808

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFISWNVNGLRACYDKGFVDVFHRLEADFFCLQETKMQEGQLDAKFEGYHSYWNYAEK
CEEEEEECCCHHHHHHHCCHHHHHHHHCCCEEEEEHHCCCCCCCCCEECCHHHHHHHHHH
KGYSGTAIFSKVKPLSVTYGLGIEEHDHEGRVITLELESFYLITVYTPNSQEELRRLDYR
CCCCCCCHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCEEEEEEECCCCHHHHHHHHCC
MKWEDDFRAYLKKLEEKKPVIVCGDLNVAHKEIDLKNPKTNRKNAGFTDEERAKFTTLLE
CCCCHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
SGFTDTFRYFYPEQEGIYSWWSYRFKAREKNSGWRIDYFLTSDSLKDRLKGAHIYTDIFG
CCCCCCEEEECCCCCCCHHHHEEEEEEECCCCCEEEEEEEECCHHHHHHCCCEEEEEECC
SDHCPVELTIEL
CCCCCEEEEEEC
>Mature Secondary Structure
MLKFISWNVNGLRACYDKGFVDVFHRLEADFFCLQETKMQEGQLDAKFEGYHSYWNYAEK
CEEEEEECCCHHHHHHHCCHHHHHHHHCCCEEEEEHHCCCCCCCCCEECCHHHHHHHHHH
KGYSGTAIFSKVKPLSVTYGLGIEEHDHEGRVITLELESFYLITVYTPNSQEELRRLDYR
CCCCCCCHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCEEEEEEECCCCHHHHHHHHCC
MKWEDDFRAYLKKLEEKKPVIVCGDLNVAHKEIDLKNPKTNRKNAGFTDEERAKFTTLLE
CCCCHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
SGFTDTFRYFYPEQEGIYSWWSYRFKAREKNSGWRIDYFLTSDSLKDRLKGAHIYTDIFG
CCCCCCEEEECCCCCCCHHHHEEEEEEECCCCCEEEEEEEECCHHHHHHCCCEEEEEECC
SDHCPVELTIEL
CCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]