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The map label for this gene is rlmN

Identifier: 150005131

GI number: 150005131

Start: 3312213

End: 3313262

Strand: Direct

Name: rlmN

Synonym: BVU_2598

Alternate gene names: 150005131

Gene position: 3312213-3313262 (Clockwise)

Preceding gene: 150005127

Following gene: 150005132

Centisome position: 64.15

GC content: 42.19

Gene sequence:

>1050_bases
ATGGAAACTCCGAAAACAGCATTACTTGGCAGGACATTGGATGAGATTCAACAGATTGTGAGGAATCTGGGAATGCCTAA
ATTTGCCGCAAAGCAGATTACGTCTTGGTTGTATGATAAAAAGGTGGAAACGATAGACGAAATGACCAATTTGTCATTAA
AGCATCGCGAAGCATTGAAAGAAGGGTATGAGGTGGGAGCATCTGCTCCGGTGGAAGAAATGCGTTCGGTAGACGGTACG
GTGAAATATCTTTTCCGTACACCGGCTCATAATTTTATTGAAGCGGTTTATATACCCGATGAGGACCGGGCTACTCTTTG
CGTTTCCTCGCAAGTGGGATGCAAGATGAATTGTAAGTTCTGTATGACCGGGAAGCAAGGTTTTACAGCTAATCTGAGTG
CCCATCAAATATTAAACCAGATCTATTCTATTCCTGAAAGGGAAAAGCTGACTAATCTTGTTTTCATGGGTATGGGAGAG
CCTTTCGACAATCTGGATGAGGTGTTGAAAGTGCTTGAGATTCTGACTTCAGAATATGGTTACGGTTGGAGCCCGAAACG
CATCACTGTTTCTTCTGTGGGTTTAAAGAAAGGACTGGAGCGCTTTCTGAATGAAAGTGATTGTCATCTGGCTATCAGTA
TGCATACTCCGATTCCTTCGCAACGAAGGGATTTAATGCCTGCCGAGAAAGCTTTTTCCATTACGGAAATTATTGATATA
TTGCATAATTATGATTTTAGCAAACAGCGGCGTCTGTCTTTTGAGTATATCGTATTCAAAGGGGTGAACGATTCACTTAT
TTACGCTAAAGAGATTGTGAAACTTTTGCGCGGCATTGAATGTCGTGTCAATTTGATCCGGTTTCATGCCATTCCTAATG
TGGATTTGGAAGGGGTAGATATGGAAACAATGGTTGCCTTCCGTGATTACTTGACGCAGCATGGCGTATTTGCTACGATC
CGTGCTTCAAGAGGAGAAGATATTTTTGCTGCTTGTGGAATGTTGTCTACGGCTAAACAACAGAAGGAGAAAGGTGTTAC
ATTACAGTAA

Upstream 100 bases:

>100_bases
ATTGTTTTTCTCATTGTTAAAAAGTATTTCTCACGGCAAAGATAGGTAATATATCTTATTTTTTCTCTACCTTTGTACGC
TAATTGTCTTTTAAATATAA

Downstream 100 bases:

>100_bases
ATAACAACAATAATACCATATAATTATGAAACGAATAGCATTTTATTTAAGTTTGGTGCTGGTGGTTCTGGTACTGGCTT
CTTGTAAGAAGGGACAAAAG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
METPKTALLGRTLDEIQQIVRNLGMPKFAAKQITSWLYDKKVETIDEMTNLSLKHREALKEGYEVGASAPVEEMRSVDGT
VKYLFRTPAHNFIEAVYIPDEDRATLCVSSQVGCKMNCKFCMTGKQGFTANLSAHQILNQIYSIPEREKLTNLVFMGMGE
PFDNLDEVLKVLEILTSEYGYGWSPKRITVSSVGLKKGLERFLNESDCHLAISMHTPIPSQRRDLMPAEKAFSITEIIDI
LHNYDFSKQRRLSFEYIVFKGVNDSLIYAKEIVKLLRGIECRVNLIRFHAIPNVDLEGVDMETMVAFRDYLTQHGVFATI
RASRGEDIFAACGMLSTAKQQKEKGVTLQ

Sequences:

>Translated_349_residues
METPKTALLGRTLDEIQQIVRNLGMPKFAAKQITSWLYDKKVETIDEMTNLSLKHREALKEGYEVGASAPVEEMRSVDGT
VKYLFRTPAHNFIEAVYIPDEDRATLCVSSQVGCKMNCKFCMTGKQGFTANLSAHQILNQIYSIPEREKLTNLVFMGMGE
PFDNLDEVLKVLEILTSEYGYGWSPKRITVSSVGLKKGLERFLNESDCHLAISMHTPIPSQRRDLMPAEKAFSITEIIDI
LHNYDFSKQRRLSFEYIVFKGVNDSLIYAKEIVKLLRGIECRVNLIRFHAIPNVDLEGVDMETMVAFRDYLTQHGVFATI
RASRGEDIFAACGMLSTAKQQKEKGVTLQ
>Mature_349_residues
METPKTALLGRTLDEIQQIVRNLGMPKFAAKQITSWLYDKKVETIDEMTNLSLKHREALKEGYEVGASAPVEEMRSVDGT
VKYLFRTPAHNFIEAVYIPDEDRATLCVSSQVGCKMNCKFCMTGKQGFTANLSAHQILNQIYSIPEREKLTNLVFMGMGE
PFDNLDEVLKVLEILTSEYGYGWSPKRITVSSVGLKKGLERFLNESDCHLAISMHTPIPSQRRDLMPAEKAFSITEIIDI
LHNYDFSKQRRLSFEYIVFKGVNDSLIYAKEIVKLLRGIECRVNLIRFHAIPNVDLEGVDMETMVAFRDYLTQHGVFATI
RASRGEDIFAACGMLSTAKQQKEKGVTLQ

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=344, Percent_Identity=35.1744186046512, Blast_Score=233, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_BACV8 (A6L3I9)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299875.1
- STRING:   A6L3I9
- GeneID:   5303561
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_2598
- NMPDR:   fig|435590.6.peg.2439
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14466
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39552; Mature: 39552

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METPKTALLGRTLDEIQQIVRNLGMPKFAAKQITSWLYDKKVETIDEMTNLSLKHREALK
CCCCHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EGYEVGASAPVEEMRSVDGTVKYLFRTPAHNFIEAVYIPDEDRATLCVSSQVGCKMNCKF
HHHHCCCCCCHHHHHCCCHHHHHHHHCCCCHHEEEEEECCCCCEEEEEECCCCCEECCEE
CMTGKQGFTANLSAHQILNQIYSIPEREKLTNLVFMGMGEPFDNLDEVLKVLEILTSEYG
EECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHCC
YGWSPKRITVSSVGLKKGLERFLNESDCHLAISMHTPIPSQRRDLMPAEKAFSITEIIDI
CCCCCCEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHH
LHNYDFSKQRRLSFEYIVFKGVNDSLIYAKEIVKLLRGIECRVNLIRFHAIPNVDLEGVD
HHCCCCCHHHCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHEEEEEEEEECCCCCCCCCC
METMVAFRDYLTQHGVFATIRASRGEDIFAACGMLSTAKQQKEKGVTLQ
HHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
METPKTALLGRTLDEIQQIVRNLGMPKFAAKQITSWLYDKKVETIDEMTNLSLKHREALK
CCCCHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EGYEVGASAPVEEMRSVDGTVKYLFRTPAHNFIEAVYIPDEDRATLCVSSQVGCKMNCKF
HHHHCCCCCCHHHHHCCCHHHHHHHHCCCCHHEEEEEECCCCCEEEEEECCCCCEECCEE
CMTGKQGFTANLSAHQILNQIYSIPEREKLTNLVFMGMGEPFDNLDEVLKVLEILTSEYG
EECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHCC
YGWSPKRITVSSVGLKKGLERFLNESDCHLAISMHTPIPSQRRDLMPAEKAFSITEIIDI
CCCCCCEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHH
LHNYDFSKQRRLSFEYIVFKGVNDSLIYAKEIVKLLRGIECRVNLIRFHAIPNVDLEGVD
HHCCCCCHHHCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHEEEEEEEEECCCCCCCCCC
METMVAFRDYLTQHGVFATIRASRGEDIFAACGMLSTAKQQKEKGVTLQ
HHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA