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The map label for this gene is sigW [H]

Identifier: 150005114

GI number: 150005114

Start: 3294256

End: 3294807

Strand: Reverse

Name: sigW [H]

Synonym: BVU_2581

Alternate gene names: 150005114

Gene position: 3294807-3294256 (Counterclockwise)

Preceding gene: 150005115

Following gene: 150005113

Centisome position: 63.81

GC content: 36.41

Gene sequence:

>552_bases
ATGAACACTTATAATGAGAAAGAAATAATAGCGTTATTACAGGACCCGGCACGACAAAAAGAAGCCTTCGAATGTATTGT
CAACGAATACAGCGAACAGCTATATTGGCAGATACGCCGGATGGTACTTTCTCATGAAGATGCCAATGATCTGTTACAGA
ATACATTTATCAAAGCATGGACTAATCTAGAGTATTTTCGTGCCGAAGCCAAAATGTCGACCTGGCTTTACCGCATAGCT
TTGAATGAATGCTTGACCTTTTTAAATAAACAACGTGCCACCAACCAATTATCCATAGACGACGCTGATGCAGATATGGT
GAACAAACTGGAAAGTAATACATATTTTAATGGAGATGAAACTCAAATATTATTTCAAAAAGCATTACTGACGCTCCCTG
AAAAGCAGCGTATAGTATTCAATTTAAAATACTTTCAAGAAATGAAATATGAAGACATGTCTGAAATATTAGGAACAAGT
ATAGGGGCACTGAAAGCCTCCTACCACCATGCAGTGAAAAAAATAGAGAACTTTTTAAAAGAATGCCTTTAA

Upstream 100 bases:

>100_bases
TATTTTTCCTAACACTCTACTTGCACGGGAAACATTAAAAATTTCAATAGAATCCATTAAACCATAACATAAACCGGATA
TCTAATTTAAAAATAGGAAC

Downstream 100 bases:

>100_bases
ACCTTTTTAAAAGAAATGTATCTAACAAAGAAAGGAGAACCACTATGAAGGAAGAAGATGAACTATTAAAAAAATGCGGG
ACAAAAAATCCATTCATGGT

Product: RNA polymerase ECF-type sigma factor

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MNTYNEKEIIALLQDPARQKEAFECIVNEYSEQLYWQIRRMVLSHEDANDLLQNTFIKAWTNLEYFRAEAKMSTWLYRIA
LNECLTFLNKQRATNQLSIDDADADMVNKLESNTYFNGDETQILFQKALLTLPEKQRIVFNLKYFQEMKYEDMSEILGTS
IGALKASYHHAVKKIENFLKECL

Sequences:

>Translated_183_residues
MNTYNEKEIIALLQDPARQKEAFECIVNEYSEQLYWQIRRMVLSHEDANDLLQNTFIKAWTNLEYFRAEAKMSTWLYRIA
LNECLTFLNKQRATNQLSIDDADADMVNKLESNTYFNGDETQILFQKALLTLPEKQRIVFNLKYFQEMKYEDMSEILGTS
IGALKASYHHAVKKIENFLKECL
>Mature_183_residues
MNTYNEKEIIALLQDPARQKEAFECIVNEYSEQLYWQIRRMVLSHEDANDLLQNTFIKAWTNLEYFRAEAKMSTWLYRIA
LNECLTFLNKQRATNQLSIDDADADMVNKLESNTYFNGDETQILFQKALLTLPEKQRIVFNLKYFQEMKYEDMSEILGTS
IGALKASYHHAVKKIENFLKECL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=175, Percent_Identity=28, Blast_Score=73, Evalue=9e-15,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21615; Mature: 21615

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTYNEKEIIALLQDPARQKEAFECIVNEYSEQLYWQIRRMVLSHEDANDLLQNTFIKAW
CCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TNLEYFRAEAKMSTWLYRIALNECLTFLNKQRATNQLSIDDADADMVNKLESNTYFNGDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCH
TQILFQKALLTLPEKQRIVFNLKYFQEMKYEDMSEILGTSIGALKASYHHAVKKIENFLK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ECL
HHC
>Mature Secondary Structure
MNTYNEKEIIALLQDPARQKEAFECIVNEYSEQLYWQIRRMVLSHEDANDLLQNTFIKAW
CCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TNLEYFRAEAKMSTWLYRIALNECLTFLNKQRATNQLSIDDADADMVNKLESNTYFNGDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCH
TQILFQKALLTLPEKQRIVFNLKYFQEMKYEDMSEILGTSIGALKASYHHAVKKIENFLK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ECL
HHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]