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The map label for this gene is ldhA [H]

Identifier: 150005034

GI number: 150005034

Start: 3198809

End: 3199816

Strand: Direct

Name: ldhA [H]

Synonym: BVU_2499

Alternate gene names: 150005034

Gene position: 3198809-3199816 (Clockwise)

Preceding gene: 150005033

Following gene: 150005036

Centisome position: 61.95

GC content: 42.16

Gene sequence:

>1008_bases
ATGGCTTATAAAATAGCTTTTTACGACACGAAACCCTACGACGAACGCTCGTTTACGGAGGCTAATGAGAAGTTTGGCTT
TGATATCAGGTATTACAAAGGCCATTTGAATATGAATAACGTGGTGTTGACCAAAGGAGTGGATGTGGTTTGTATCTTCG
TAAACGATACGGCTGATGCCGAAGTTATCCGGGCAATGGCGGATAATGGTGTTAAACTGCTGGCTTTAAGATGCGCAGGT
TATAATAATGTGGATTTAGCGGCAACGGCAGGTAAGATGAAAGTGGTACGGGTACCGGCTTATTCACCTTATGCCGTTGC
CGAATTTACGGTGGCGTTGATGTTATCACTCAACCGCAAGATTCCCCGTGCTACAATGCGGACACGTGACGGGAATTTCT
CCCTGCACGGGCTTATGGGATTTGATATGCATGGCAAGACTGCCGGAATTATCGGTACGGGTAAGATTGCCAAGATTTTA
ATTCAGATATTACGTGGATTCGGTATGAATGTTCTTGCATATGATCTTTATCCCGATTACAATTTTGCCCGTGAACATCA
GGTGGTTTATTGTACTTTGGATGAACTTTATCATAGTTCGGATATCATCTCCCTGCATTGTCCTTTGACAGAACAGACAA
AGTATCTGATTAATGATTATTCCATCAGTAAAATGAAAGACGGAGTGATGATTATCAATACGGGGCGTGGGCAGTTGATT
CACACCAATGCCTTGATAGAGGGACTGAAGACTAAAAAGGTAGGATATGCCGGTCTGGATGTATACGAGGAAGAAGAGCC
TTATTTTTATGAAGATAAGTCCGACAAGATTATTGATGATGACACTTTAGCCCGTCTTTTGTCTTTCAACAATGTGATTG
TGACTTCTCATCAGGCATTCTTTACCAAAGAAGCAATGACGAATATTGCTCATACTACTCTGCAAAATGTGAAAGACTTT
GCTGAAAGTAGATCTTTGGTGAACGAAGTAGCGGTGGGGAGAGTCTAA

Upstream 100 bases:

>100_bases
ACAGCGCCTGTACACGGAACTGAAAAAACTATAAGTCAGTATGTGAACAGTAGGGGCCGTGTTTTTGTTATTTATATGAA
CTATAAATGGAAAAACAATT

Downstream 100 bases:

>100_bases
TCTCCCCTGTAGTAATATAATAAGGAGTTGCGGGCTATAAAGTCCGCATTCTTTTGAACCAGTTCCCTTTCACCAAATTC
GGGGAATTGTGCATCGGGGA

Product: putative dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAYKIAFYDTKPYDERSFTEANEKFGFDIRYYKGHLNMNNVVLTKGVDVVCIFVNDTADAEVIRAMADNGVKLLALRCAG
YNNVDLAATAGKMKVVRVPAYSPYAVAEFTVALMLSLNRKIPRATMRTRDGNFSLHGLMGFDMHGKTAGIIGTGKIAKIL
IQILRGFGMNVLAYDLYPDYNFAREHQVVYCTLDELYHSSDIISLHCPLTEQTKYLINDYSISKMKDGVMIINTGRGQLI
HTNALIEGLKTKKVGYAGLDVYEEEEPYFYEDKSDKIIDDDTLARLLSFNNVIVTSHQAFFTKEAMTNIAHTTLQNVKDF
AESRSLVNEVAVGRV

Sequences:

>Translated_335_residues
MAYKIAFYDTKPYDERSFTEANEKFGFDIRYYKGHLNMNNVVLTKGVDVVCIFVNDTADAEVIRAMADNGVKLLALRCAG
YNNVDLAATAGKMKVVRVPAYSPYAVAEFTVALMLSLNRKIPRATMRTRDGNFSLHGLMGFDMHGKTAGIIGTGKIAKIL
IQILRGFGMNVLAYDLYPDYNFAREHQVVYCTLDELYHSSDIISLHCPLTEQTKYLINDYSISKMKDGVMIINTGRGQLI
HTNALIEGLKTKKVGYAGLDVYEEEEPYFYEDKSDKIIDDDTLARLLSFNNVIVTSHQAFFTKEAMTNIAHTTLQNVKDF
AESRSLVNEVAVGRV
>Mature_334_residues
AYKIAFYDTKPYDERSFTEANEKFGFDIRYYKGHLNMNNVVLTKGVDVVCIFVNDTADAEVIRAMADNGVKLLALRCAGY
NNVDLAATAGKMKVVRVPAYSPYAVAEFTVALMLSLNRKIPRATMRTRDGNFSLHGLMGFDMHGKTAGIIGTGKIAKILI
QILRGFGMNVLAYDLYPDYNFAREHQVVYCTLDELYHSSDIISLHCPLTEQTKYLINDYSISKMKDGVMIINTGRGQLIH
TNALIEGLKTKKVGYAGLDVYEEEEPYFYEDKSDKIIDDDTLARLLSFNNVIVTSHQAFFTKEAMTNIAHTTLQNVKDFA
ESRSLVNEVAVGRV

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=252, Percent_Identity=30.952380952381, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI61743967, Length=251, Percent_Identity=29.8804780876494, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI4557497, Length=251, Percent_Identity=29.8804780876494, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI145580578, Length=238, Percent_Identity=31.0924369747899, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI4557499, Length=238, Percent_Identity=31.0924369747899, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI145580575, Length=250, Percent_Identity=31.2, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI6912396, Length=225, Percent_Identity=29.7777777777778, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=50.3048780487805, Blast_Score=337, Evalue=5e-94,
Organism=Escherichia coli, GI87082289, Length=257, Percent_Identity=32.295719844358, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789279, Length=242, Percent_Identity=32.2314049586777, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17532191, Length=274, Percent_Identity=29.9270072992701, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI25147481, Length=240, Percent_Identity=29.5833333333333, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320925, Length=290, Percent_Identity=30.6896551724138, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6322116, Length=287, Percent_Identity=31.0104529616725, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324055, Length=247, Percent_Identity=31.9838056680162, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324964, Length=230, Percent_Identity=25.6521739130435, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921140, Length=298, Percent_Identity=28.5234899328859, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24646446, Length=318, Percent_Identity=28.6163522012579, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24646448, Length=318, Percent_Identity=28.6163522012579, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24646452, Length=318, Percent_Identity=28.6163522012579, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24646450, Length=318, Percent_Identity=28.6163522012579, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI62472511, Length=309, Percent_Identity=29.4498381877023, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28574286, Length=285, Percent_Identity=26.6666666666667, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24585514, Length=279, Percent_Identity=27.2401433691756, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28574282, Length=279, Percent_Identity=27.2401433691756, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45552429, Length=279, Percent_Identity=27.2401433691756, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28574284, Length=279, Percent_Identity=27.2401433691756, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45551003, Length=279, Percent_Identity=27.2401433691756, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28571528, Length=295, Percent_Identity=27.4576271186441, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 37604; Mature: 37472

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYKIAFYDTKPYDERSFTEANEKFGFDIRYYKGHLNMNNVVLTKGVDVVCIFVNDTADA
CEEEEEEECCCCCCCCCHHCCCHHCCCEEEEEECCCCCCCEEEECCCEEEEEEECCCCCH
EVIRAMADNGVKLLALRCAGYNNVDLAATAGKMKVVRVPAYSPYAVAEFTVALMLSLNRK
HHHHHHHCCCEEEEEEEECCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCC
IPRATMRTRDGNFSLHGLMGFDMHGKTAGIIGTGKIAKILIQILRGFGMNVLAYDLYPDY
CCCHHHEECCCCEEEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCC
NFAREHQVVYCTLDELYHSSDIISLHCPLTEQTKYLINDYSISKMKDGVMIINTGRGQLI
CCCCCCEEEEEEHHHHHCCCCEEEEECCCCCCCHHEECCCCHHHHCCCEEEEECCCCCEE
HTNALIEGLKTKKVGYAGLDVYEEEEPYFYEDKSDKIIDDDTLARLLSFNNVIVTSHQAF
EHHHHHHCHHHCCCCCCCCCEEECCCCEEEECCCCCEECHHHHHHHHHCCCEEEECCHHH
FTKEAMTNIAHTTLQNVKDFAESRSLVNEVAVGRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AYKIAFYDTKPYDERSFTEANEKFGFDIRYYKGHLNMNNVVLTKGVDVVCIFVNDTADA
EEEEEEECCCCCCCCCHHCCCHHCCCEEEEEECCCCCCCEEEECCCEEEEEEECCCCCH
EVIRAMADNGVKLLALRCAGYNNVDLAATAGKMKVVRVPAYSPYAVAEFTVALMLSLNRK
HHHHHHHCCCEEEEEEEECCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCC
IPRATMRTRDGNFSLHGLMGFDMHGKTAGIIGTGKIAKILIQILRGFGMNVLAYDLYPDY
CCCHHHEECCCCEEEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCC
NFAREHQVVYCTLDELYHSSDIISLHCPLTEQTKYLINDYSISKMKDGVMIINTGRGQLI
CCCCCCEEEEEEHHHHHCCCCEEEEECCCCCCCHHEECCCCHHHHCCCEEEEECCCCCEE
HTNALIEGLKTKKVGYAGLDVYEEEEPYFYEDKSDKIIDDDTLARLLSFNNVIVTSHQAF
EHHHHHHCHHHCCCCCCCCCEEECCCCEEEECCCCCEECHHHHHHHHHCCCEEEECCHHH
FTKEAMTNIAHTTLQNVKDFAESRSLVNEVAVGRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]