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The map label for this gene is trpB2 [H]

Identifier: 150004975

GI number: 150004975

Start: 3134961

End: 3136325

Strand: Reverse

Name: trpB2 [H]

Synonym: BVU_2439

Alternate gene names: 150004975

Gene position: 3136325-3134961 (Counterclockwise)

Preceding gene: 150004980

Following gene: 150004974

Centisome position: 60.74

GC content: 47.91

Gene sequence:

>1365_bases
ATGAGCACAAAACAGAAACGTTTTATCTTACCAGAGGATGAAATTCCTAAATACTGGTACAACATACAGGCAGACATGAA
GACCAAACCCATGCCGCCGTTGAACCCTAAAACCAAAGAAGCGCTGAAACCGGAAGACCTCTACCCCATTTTTGCCAAAG
AACTTTGCAAACAGGAATTAAACCAGACAGACGCATGGATTGAAATTCCCGAAGCAGTGCGTGAAATGTACAAATACTAT
CGCAGCACACCATTGGTACGTGCCTACGGACTGGAAAAAGCACTAGGGACTCCCGCACATATTTATTTCAAAAATGAAAG
TGTCAGCCCCATCGGATCCCACAAACTGAACTCGGCTCTGGCCCAGGCATATTATTGCAAGGAAGAAGGCGTAACCAATG
TCACTACAGAAACCGGTGCCGGACAATGGGGCGCCGCTCTATCGTATGCCGCAAAGGTATTCGGGCTGGAAGCTGCCGTT
TATCAAGTAAAGATCAGCTATAATCAGAAGCCCTATCGCCGCAGCATCATGCAGACATTCGGAGCAACGGTCACCGCCTC
TCCATCCATGTCTACCCGTGCCGGCAAGGATATCATTACCCGTAATCCCAATTATCAAGGTTCACTGGGAACAGCCATCT
CCGAAGCTATCGAACTGGCAATGAGCACTCCCAACTGTAAATATACACTAGGGTCGGTATTAAGCCATGTCACACTGCAT
CAAACGGTTATCGGTCTGGAAGCCGAAAAGCAAATGGAAATGGCAGGCGAATATCCCGACATGATTATCGGTTGCTTTGG
TGGCGGTTCCAATTTTGGAGGAATCTGCTTCCCCTTTATGCGCCACACTATCCTGAATGGGAAACAAACCCGATACATTG
CTGCCGAACCGGCTTCGTGCCCGAAACTGACCCGTGGTAAATTCCAGTATGACTTTGGCGACGAAGCCGGATATACCCCG
TTGCTGCCCATGTTTACACTGGGGCATAATTTCTCTCCGGCCAATATCCATGCGGGAGGTCTCCGTTATCACGGTGCAGG
TGTCATCGTTTCACAATTATTGAAAGACAATCTGATGGAAGCGGTTGATATCCAGCAATTAGAATCCTTCCAGGCTGGTT
GTTTGTTCGCACAAGCAGAAGGAATCATTCCGGCTCCCGAATCGTGCCATGCCATCGCCGCCGCTGTACGCGAGGCGAAC
AAGTGCAAAGAATCCGGTGAAGAGAAAGTGATCCTGTTCAACCTTTCAGGTCACGGGCTGATTGATATGGCCTCATACGA
CCAATACCTGGCAGGTAACTTGATGAATTATGAGTTAAAAGACGAAGATATCCAGAAGAACTTGGATGAAATAAAAGACA
TGTAA

Upstream 100 bases:

>100_bases
ATCCCTTTTTTTATTGTTCCCGACAAAAAAGCAGTTGAAAATTAGTAAGGAATACTTTTTTTACCTAATTTTGACCGCAA
AACTATTAAATTGAAAGATT

Downstream 100 bases:

>100_bases
TCAGGACATAAAATAAACGAAGGATACGGAAAGGATATATTATCCTATAAACTACGGCAAGCTAAGCGGTTGTATCCTTC
AGGTTAAGGGTTCTTTTACT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSTKQKRFILPEDEIPKYWYNIQADMKTKPMPPLNPKTKEALKPEDLYPIFAKELCKQELNQTDAWIEIPEAVREMYKYY
RSTPLVRAYGLEKALGTPAHIYFKNESVSPIGSHKLNSALAQAYYCKEEGVTNVTTETGAGQWGAALSYAAKVFGLEAAV
YQVKISYNQKPYRRSIMQTFGATVTASPSMSTRAGKDIITRNPNYQGSLGTAISEAIELAMSTPNCKYTLGSVLSHVTLH
QTVIGLEAEKQMEMAGEYPDMIIGCFGGGSNFGGICFPFMRHTILNGKQTRYIAAEPASCPKLTRGKFQYDFGDEAGYTP
LLPMFTLGHNFSPANIHAGGLRYHGAGVIVSQLLKDNLMEAVDIQQLESFQAGCLFAQAEGIIPAPESCHAIAAAVREAN
KCKESGEEKVILFNLSGHGLIDMASYDQYLAGNLMNYELKDEDIQKNLDEIKDM

Sequences:

>Translated_454_residues
MSTKQKRFILPEDEIPKYWYNIQADMKTKPMPPLNPKTKEALKPEDLYPIFAKELCKQELNQTDAWIEIPEAVREMYKYY
RSTPLVRAYGLEKALGTPAHIYFKNESVSPIGSHKLNSALAQAYYCKEEGVTNVTTETGAGQWGAALSYAAKVFGLEAAV
YQVKISYNQKPYRRSIMQTFGATVTASPSMSTRAGKDIITRNPNYQGSLGTAISEAIELAMSTPNCKYTLGSVLSHVTLH
QTVIGLEAEKQMEMAGEYPDMIIGCFGGGSNFGGICFPFMRHTILNGKQTRYIAAEPASCPKLTRGKFQYDFGDEAGYTP
LLPMFTLGHNFSPANIHAGGLRYHGAGVIVSQLLKDNLMEAVDIQQLESFQAGCLFAQAEGIIPAPESCHAIAAAVREAN
KCKESGEEKVILFNLSGHGLIDMASYDQYLAGNLMNYELKDEDIQKNLDEIKDM
>Mature_453_residues
STKQKRFILPEDEIPKYWYNIQADMKTKPMPPLNPKTKEALKPEDLYPIFAKELCKQELNQTDAWIEIPEAVREMYKYYR
STPLVRAYGLEKALGTPAHIYFKNESVSPIGSHKLNSALAQAYYCKEEGVTNVTTETGAGQWGAALSYAAKVFGLEAAVY
QVKISYNQKPYRRSIMQTFGATVTASPSMSTRAGKDIITRNPNYQGSLGTAISEAIELAMSTPNCKYTLGSVLSHVTLHQ
TVIGLEAEKQMEMAGEYPDMIIGCFGGGSNFGGICFPFMRHTILNGKQTRYIAAEPASCPKLTRGKFQYDFGDEAGYTPL
LPMFTLGHNFSPANIHAGGLRYHGAGVIVSQLLKDNLMEAVDIQQLESFQAGCLFAQAEGIIPAPESCHAIAAAVREANK
CKESGEEKVILFNLSGHGLIDMASYDQYLAGNLMNYELKDEDIQKNLDEIKDM

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=380, Percent_Identity=28.4210526315789, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321412, Length=418, Percent_Identity=29.4258373205742, Blast_Score=113, Evalue=6e-26,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 50194; Mature: 50063

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKQKRFILPEDEIPKYWYNIQADMKTKPMPPLNPKTKEALKPEDLYPIFAKELCKQEL
CCCCCCCEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHH
NQTDAWIEIPEAVREMYKYYRSTPLVRAYGLEKALGTPAHIYFKNESVSPIGSHKLNSAL
CCCCCEEECHHHHHHHHHHHHCCCCEEHHCHHHHCCCCEEEEECCCCCCCCCHHHHHHHH
AQAYYCKEEGVTNVTTETGAGQWGAALSYAAKVFGLEAAVYQVKISYNQKPYRRSIMQTF
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHH
GATVTASPSMSTRAGKDIITRNPNYQGSLGTAISEAIELAMSTPNCKYTLGSVLSHVTLH
CCEEECCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
QTVIGLEAEKQMEMAGEYPDMIIGCFGGGSNFGGICFPFMRHTILNGKQTRYIAAEPASC
HHHHCCCHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCC
PKLTRGKFQYDFGDEAGYTPLLPMFTLGHNFSPANIHAGGLRYHGAGVIVSQLLKDNLME
CCCCCCCEEECCCCCCCCCCCCHHHHCCCCCCCCCEECCCEEECCHHHHHHHHHHHHHHH
AVDIQQLESFQAGCLFAQAEGIIPAPESCHAIAAAVREANKCKESGEEKVILFNLSGHGL
HHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCE
IDMASYDQYLAGNLMNYELKDEDIQKNLDEIKDM
EEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
STKQKRFILPEDEIPKYWYNIQADMKTKPMPPLNPKTKEALKPEDLYPIFAKELCKQEL
CCCCCCEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHH
NQTDAWIEIPEAVREMYKYYRSTPLVRAYGLEKALGTPAHIYFKNESVSPIGSHKLNSAL
CCCCCEEECHHHHHHHHHHHHCCCCEEHHCHHHHCCCCEEEEECCCCCCCCCHHHHHHHH
AQAYYCKEEGVTNVTTETGAGQWGAALSYAAKVFGLEAAVYQVKISYNQKPYRRSIMQTF
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHH
GATVTASPSMSTRAGKDIITRNPNYQGSLGTAISEAIELAMSTPNCKYTLGSVLSHVTLH
CCEEECCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
QTVIGLEAEKQMEMAGEYPDMIIGCFGGGSNFGGICFPFMRHTILNGKQTRYIAAEPASC
HHHHCCCHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCC
PKLTRGKFQYDFGDEAGYTPLLPMFTLGHNFSPANIHAGGLRYHGAGVIVSQLLKDNLME
CCCCCCCEEECCCCCCCCCCCCHHHHCCCCCCCCCEECCCEEECCHHHHHHHHHHHHHHH
AVDIQQLESFQAGCLFAQAEGIIPAPESCHAIAAAVREANKCKESGEEKVILFNLSGHGL
HHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCE
IDMASYDQYLAGNLMNYELKDEDIQKNLDEIKDM
EEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]