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The map label for this gene is sigW [H]

Identifier: 150004875

GI number: 150004875

Start: 3015462

End: 3015998

Strand: Direct

Name: sigW [H]

Synonym: BVU_2338

Alternate gene names: 150004875

Gene position: 3015462-3015998 (Clockwise)

Preceding gene: 150004872

Following gene: 150004876

Centisome position: 58.4

GC content: 39.29

Gene sequence:

>537_bases
ATGATAAACGAAGATAAAATTAGGGAAGTATGGGCTTCCAACCCGGAACGGGGATTTAAATTGTTGATAGATTTTTTTCA
ACAGCCCATATATTGGCATATCCGGAGATTGGTTGTATCACATGAGGATGCAGAGGATATTCTGCAGGAAGTCTTTATTC
GCGTCTTTCGCCATCTTCCTCAGTTTAGGGAGGAAAGTTCATTGGGGACATGGATTTACCGGATAGCGACCAATGAGTGT
CTCCGTTTTCTGAATATGCGTAAGGAACAAGCTGTTTCGGCGGAAGAAGTTCAGGAAGAGTTAATGAATAAGTTGATGGC
GTCAGATTATGTGGATTATGAGAACGAAATGGCGGTGAAATTTCAGCAGGCTATTTTGATGCTTCCTGAAAAGCAGCGGG
TAGTTTTCAATCTTCGTTATTATGATGAACTGGAATATGAGGAAATTAGCAGGATTACGGACACGAAAGTGGATACGTTG
AAAGTGAATTATCATTATGCAAAAGAGAAGATAAAAGAGTACATGATAAATAATTAA

Upstream 100 bases:

>100_bases
TGATTATTTTAACTTCATCTATAATTAATGTAAAAACTTCATTTTCACTTTTAACTTCTTGTTCTCGGGTGGTTCTAACC
TATAAACTTTTGACGAATAG

Downstream 100 bases:

>100_bases
AGACAGGATGGAAAAGGAGTTTGATTTTAAGAATATTGGGAAGCGGATGCCTTATCGTACCCCTGATGGCTTTTTCGGAA
AAATGCAGAAGCAAGTATTG

Product: RNA polymerase ECF-type sigma factor

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MINEDKIREVWASNPERGFKLLIDFFQQPIYWHIRRLVVSHEDAEDILQEVFIRVFRHLPQFREESSLGTWIYRIATNEC
LRFLNMRKEQAVSAEEVQEELMNKLMASDYVDYENEMAVKFQQAILMLPEKQRVVFNLRYYDELEYEEISRITDTKVDTL
KVNYHYAKEKIKEYMINN

Sequences:

>Translated_178_residues
MINEDKIREVWASNPERGFKLLIDFFQQPIYWHIRRLVVSHEDAEDILQEVFIRVFRHLPQFREESSLGTWIYRIATNEC
LRFLNMRKEQAVSAEEVQEELMNKLMASDYVDYENEMAVKFQQAILMLPEKQRVVFNLRYYDELEYEEISRITDTKVDTL
KVNYHYAKEKIKEYMINN
>Mature_178_residues
MINEDKIREVWASNPERGFKLLIDFFQQPIYWHIRRLVVSHEDAEDILQEVFIRVFRHLPQFREESSLGTWIYRIATNEC
LRFLNMRKEQAVSAEEVQEELMNKLMASDYVDYENEMAVKFQQAILMLPEKQRVVFNLRYYDELEYEEISRITDTKVDTL
KVNYHYAKEKIKEYMINN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=164, Percent_Identity=31.7073170731707, Blast_Score=74, Evalue=4e-15,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21671; Mature: 21671

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINEDKIREVWASNPERGFKLLIDFFQQPIYWHIRRLVVSHEDAEDILQEVFIRVFRHLP
CCCHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QFREESSLGTWIYRIATNECLRFLNMRKEQAVSAEEVQEELMNKLMASDYVDYENEMAVK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
FQQAILMLPEKQRVVFNLRYYDELEYEEISRITDTKVDTLKVNYHYAKEKIKEYMINN
HHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINEDKIREVWASNPERGFKLLIDFFQQPIYWHIRRLVVSHEDAEDILQEVFIRVFRHLP
CCCHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QFREESSLGTWIYRIATNECLRFLNMRKEQAVSAEEVQEELMNKLMASDYVDYENEMAVK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
FQQAILMLPEKQRVVFNLRYYDELEYEEISRITDTKVDTLKVNYHYAKEKIKEYMINN
HHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]