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The map label for this gene is grpE [H]

Identifier: 150004867

GI number: 150004867

Start: 3007513

End: 3008133

Strand: Reverse

Name: grpE [H]

Synonym: BVU_2330

Alternate gene names: 150004867

Gene position: 3008133-3007513 (Counterclockwise)

Preceding gene: 150004870

Following gene: 150004866

Centisome position: 58.26

GC content: 39.77

Gene sequence:

>621_bases
ATGAGTAAAAATAAGAAAAACAAAAAGTTTAATAAGAATATGAACCCAACAGAGAAGAACCAACCTCAAGACGAGGAAGT
TTTGAAAAATCAGGAAGCGGCAGAAGCTGCCATTGATGAAGAAACTCAGAAAGAAGCAACAGAAGAACTGAACGCTGAAG
AGAAGGTGAACAAGGAATTGGCGGAAGCTCAGAAAACAATAGAAGAGCAGCATGACAAATACCTGCGTCTTTCAGCCGAA
TTTGACAATTACCGTAAACGCACCATGAAAGAGAAGGCCGAATTGATTAAAAATGGTGGAGAAAAGGCCATTACTGCCAT
TCTTCCTATTTTGGATGACTTGGAGCGTGCAGTAAAGACTTCAGAAACTTCGGATGATGTAAAAGCAATGCGTGAAGGAA
TTGAGTTGATCTACAACAAATTCCTGAAAGTATTGAACCAGGAAGGACTTCAGAAAATAGAGACTGACGGTGAGAACTTT
GATACCGATTATCACGAAGCAATTGCATTAGTTCCAGCTCCTTCCGAAGAGAAGAAAGGAAAAATATTGGACTGTGTACA
AACCGGTTATAAGCTGAATGACAAAGTAATACGCCACGCCAAAGTAGTTGTGGCTCAATAA

Upstream 100 bases:

>100_bases
GTGTGGCAGAAATTCCGTACTTTTGCAAAATTATTACTCTAAGAAAACAACTAGGCACGAAATTTGTTAGCAGTAAATTA
ACCTAAAAGACCTGACTGTT

Downstream 100 bases:

>100_bases
AACAGTATCATAAAACGACATGGCAAAAAGAGACTATTACGAAGTTCTGGAGGTGGACAAAACAGCCACCCTTGATGTTA
TAAAAAAAGCATACCGCAAA

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSKNKKNKKFNKNMNPTEKNQPQDEEVLKNQEAAEAAIDEETQKEATEELNAEEKVNKELAEAQKTIEEQHDKYLRLSAE
FDNYRKRTMKEKAELIKNGGEKAITAILPILDDLERAVKTSETSDDVKAMREGIELIYNKFLKVLNQEGLQKIETDGENF
DTDYHEAIALVPAPSEEKKGKILDCVQTGYKLNDKVIRHAKVVVAQ

Sequences:

>Translated_206_residues
MSKNKKNKKFNKNMNPTEKNQPQDEEVLKNQEAAEAAIDEETQKEATEELNAEEKVNKELAEAQKTIEEQHDKYLRLSAE
FDNYRKRTMKEKAELIKNGGEKAITAILPILDDLERAVKTSETSDDVKAMREGIELIYNKFLKVLNQEGLQKIETDGENF
DTDYHEAIALVPAPSEEKKGKILDCVQTGYKLNDKVIRHAKVVVAQ
>Mature_205_residues
SKNKKNKKFNKNMNPTEKNQPQDEEVLKNQEAAEAAIDEETQKEATEELNAEEKVNKELAEAQKTIEEQHDKYLRLSAEF
DNYRKRTMKEKAELIKNGGEKAITAILPILDDLERAVKTSETSDDVKAMREGIELIYNKFLKVLNQEGLQKIETDGENFD
TDYHEAIALVPAPSEEKKGKILDCVQTGYKLNDKVIRHAKVVVAQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=200, Percent_Identity=33.5, Blast_Score=88, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324806, Length=171, Percent_Identity=28.0701754385965, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 23521; Mature: 23390

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNKKNKKFNKNMNPTEKNQPQDEEVLKNQEAAEAAIDEETQKEATEELNAEEKVNKEL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AEAQKTIEEQHDKYLRLSAEFDNYRKRTMKEKAELIKNGGEKAITAILPILDDLERAVKT
HHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
SETSDDVKAMREGIELIYNKFLKVLNQEGLQKIETDGENFDTDYHEAIALVPAPSEEKKG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEECCCCCCHHCC
KILDCVQTGYKLNDKVIRHAKVVVAQ
HHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKNKKNKKFNKNMNPTEKNQPQDEEVLKNQEAAEAAIDEETQKEATEELNAEEKVNKEL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AEAQKTIEEQHDKYLRLSAEFDNYRKRTMKEKAELIKNGGEKAITAILPILDDLERAVKT
HHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
SETSDDVKAMREGIELIYNKFLKVLNQEGLQKIETDGENFDTDYHEAIALVPAPSEEKKG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEECCCCCCHHCC
KILDCVQTGYKLNDKVIRHAKVVVAQ
HHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]