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The map label for this gene is afr [H]

Identifier: 150004844

GI number: 150004844

Start: 2981865

End: 2982854

Strand: Reverse

Name: afr [H]

Synonym: BVU_2307

Alternate gene names: 150004844

Gene position: 2982854-2981865 (Counterclockwise)

Preceding gene: 150004845

Following gene: 150004843

Centisome position: 57.77

GC content: 48.38

Gene sequence:

>990_bases
ATGGTTAAATTAGACAAAGTAAAATGGGGCTTCATCGGTTGCGGAGAAGTGACCGAGAAGAAAAGCGGACCAGCCTTTGC
CCTGATCGAAGGTTCAGAGGTAGTGGCCGTAATGAGCCGTGACAAAGAAAAAGCTCAATCATACGCCAAAAGACACAACA
TTCCCCGCTGGTACTCCGATCCGCAGCAGTTAATAGGAGATGAAGATGTCAATGCTATTTATATAGCCACTCCCCCCTCT
TCTCATGCCACTTTTGCCATTATGTCCATGAAGGCAGGAAAGCCCGTATACATCGAGAAACCTATGGCGGCCAGCTATGA
AGACTGTGCACGCATCAACCGCATATCACAGGAAACAGGAATTCCTTGTTTCGTAGCTTATTACAGACGCTATCTTCCTT
ATTTCCAAAAAGTACGCCAGATGGTAGAGGACGGAGAAATAGGCAATGTCATCAACATACAGATACGTTTCGCCCAGCCT
CCCCGCGCTTTGGATTACAACAGTACCAACCTTCCCTGGCGTGTACAACCTGATATTGCCGGCGGCGGTTATTTCTATGA
TCTGGCTCCTCACCAGCTGGATTTGCTACAAGATATGTTCGGCTGCATTCTCGAAGCCCAAGGATACAAGAGCAACCGTG
GCGGGTTATATCCGGCAGAAGATACGATAAGTGCCTGCTTCAAATTTGATTCCGGTCTGCCGGGATCAGGATCATGGTGT
TTCGTTGCCCATGAATCGGCCAAGGAAGACCGTATAGAAATCATCGGAGACAAGGGAATGATCTGTTTCTCTGTTTTCAC
TTACGATCCCATCGCCCTGCATACTGAAAGAGGCCGCGAGGAGTTTCTTCCCGAAAATCCCCCTCATGTACAACTTCCGC
TCATAAAAGCCGTAGTAGAACACTTGCAGGGAAAAGCTGTCTGTACCTGCGACGGCATCAGTGCGACACCCACCAACTGG
GTAATGGACCGTATACTTGACAAACTCTGA

Upstream 100 bases:

>100_bases
TACAATAACTGATTTTTATCCGGCTGAAATTGCAAAATAGTCCTATCTTTGCAATATAAATTGTTATTATTTTAAGCGGA
ATGACAAAAAAGTTATTACC

Downstream 100 bases:

>100_bases
TTTCTGCCTATGGACATCCGTCAATTATGCCTGTTTTGTCTGTCTTTCCTTTCCGCCCTCGGTGTATATGCACAAGCGGA
ACAGAAGGACTCGACAGTAC

Product: GFO/IDH/MocA family oxidoreductase

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MVKLDKVKWGFIGCGEVTEKKSGPAFALIEGSEVVAVMSRDKEKAQSYAKRHNIPRWYSDPQQLIGDEDVNAIYIATPPS
SHATFAIMSMKAGKPVYIEKPMAASYEDCARINRISQETGIPCFVAYYRRYLPYFQKVRQMVEDGEIGNVINIQIRFAQP
PRALDYNSTNLPWRVQPDIAGGGYFYDLAPHQLDLLQDMFGCILEAQGYKSNRGGLYPAEDTISACFKFDSGLPGSGSWC
FVAHESAKEDRIEIIGDKGMICFSVFTYDPIALHTERGREEFLPENPPHVQLPLIKAVVEHLQGKAVCTCDGISATPTNW
VMDRILDKL

Sequences:

>Translated_329_residues
MVKLDKVKWGFIGCGEVTEKKSGPAFALIEGSEVVAVMSRDKEKAQSYAKRHNIPRWYSDPQQLIGDEDVNAIYIATPPS
SHATFAIMSMKAGKPVYIEKPMAASYEDCARINRISQETGIPCFVAYYRRYLPYFQKVRQMVEDGEIGNVINIQIRFAQP
PRALDYNSTNLPWRVQPDIAGGGYFYDLAPHQLDLLQDMFGCILEAQGYKSNRGGLYPAEDTISACFKFDSGLPGSGSWC
FVAHESAKEDRIEIIGDKGMICFSVFTYDPIALHTERGREEFLPENPPHVQLPLIKAVVEHLQGKAVCTCDGISATPTNW
VMDRILDKL
>Mature_329_residues
MVKLDKVKWGFIGCGEVTEKKSGPAFALIEGSEVVAVMSRDKEKAQSYAKRHNIPRWYSDPQQLIGDEDVNAIYIATPPS
SHATFAIMSMKAGKPVYIEKPMAASYEDCARINRISQETGIPCFVAYYRRYLPYFQKVRQMVEDGEIGNVINIQIRFAQP
PRALDYNSTNLPWRVQPDIAGGGYFYDLAPHQLDLLQDMFGCILEAQGYKSNRGGLYPAEDTISACFKFDSGLPGSGSWC
FVAHESAKEDRIEIIGDKGMICFSVFTYDPIALHTERGREEFLPENPPHVQLPLIKAVVEHLQGKAVCTCDGISATPTNW
VMDRILDKL

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=287, Percent_Identity=22.6480836236934, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI87081947, Length=199, Percent_Identity=27.1356783919598, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082405, Length=186, Percent_Identity=24.7311827956989, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789848, Length=195, Percent_Identity=24.1025641025641, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787574, Length=330, Percent_Identity=22.7272727272727, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323975, Length=195, Percent_Identity=28.7179487179487, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24581117, Length=290, Percent_Identity=26.551724137931, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24581115, Length=202, Percent_Identity=25.7425742574257, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 36845; Mature: 36845

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLDKVKWGFIGCGEVTEKKSGPAFALIEGSEVVAVMSRDKEKAQSYAKRHNIPRWYSD
CCEECCEEECEEECCCHHCCCCCCEEEEEECCEEEEEECCCHHHHHHHHHHCCCCCCCCC
PQQLIGDEDVNAIYIATPPSSHATFAIMSMKAGKPVYIEKPMAASYEDCARINRISQETG
HHHHCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCC
IPCFVAYYRRYLPYFQKVRQMVEDGEIGNVINIQIRFAQPPRALDYNSTNLPWRVQPDIA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCC
GGGYFYDLAPHQLDLLQDMFGCILEAQGYKSNRGGLYPAEDTISACFKFDSGLPGSGSWC
CCEEEEECCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEE
FVAHESAKEDRIEIIGDKGMICFSVFTYDPIALHTERGREEFLPENPPHVQLPLIKAVVE
EEEECCCCCCCEEEECCCCEEEEEEECCCCEEEECCCCHHHCCCCCCCEEEHHHHHHHHH
HLQGKAVCTCDGISATPTNWVMDRILDKL
HHCCCEEEEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MVKLDKVKWGFIGCGEVTEKKSGPAFALIEGSEVVAVMSRDKEKAQSYAKRHNIPRWYSD
CCEECCEEECEEECCCHHCCCCCCEEEEEECCEEEEEECCCHHHHHHHHHHCCCCCCCCC
PQQLIGDEDVNAIYIATPPSSHATFAIMSMKAGKPVYIEKPMAASYEDCARINRISQETG
HHHHCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCC
IPCFVAYYRRYLPYFQKVRQMVEDGEIGNVINIQIRFAQPPRALDYNSTNLPWRVQPDIA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCC
GGGYFYDLAPHQLDLLQDMFGCILEAQGYKSNRGGLYPAEDTISACFKFDSGLPGSGSWC
CCEEEEECCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEE
FVAHESAKEDRIEIIGDKGMICFSVFTYDPIALHTERGREEFLPENPPHVQLPLIKAVVE
EEEECCCCCCCEEEECCCCEEEEEEECCCCEEEECCCCHHHCCCCCCCEEEHHHHHHHHH
HLQGKAVCTCDGISATPTNWVMDRILDKL
HHCCCEEEEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA