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The map label for this gene is queF [H]

Identifier: 150004785

GI number: 150004785

Start: 2907448

End: 2907903

Strand: Direct

Name: queF [H]

Synonym: BVU_2247

Alternate gene names: 150004785

Gene position: 2907448-2907903 (Clockwise)

Preceding gene: 150004784

Following gene: 150004787

Centisome position: 56.31

GC content: 43.2

Gene sequence:

>456_bases
ATGGAAAGAAAAGATGAATTGACCCTTCTCGGGTCGAAGACCGAGTACAGGCAAGATTATGCTCCCGAAGTGTTGGAGTC
CTTTGTGAACAAACATCCCGGAAACGACTATTGGGTACGTTTTAACTGTCCCGAATTTACCAGTTTGTGTCCTATTACAG
GACAGCCCGATTTTGCGGAAATCCGTATCTCCTACCTGCCTGATGTGAAAATGGTGGAGAGCAAGAGCTTGAAGCTGTAT
TTGTTTAGTTTCCGTAATCATGGCGATTTTCATGAAGATTGCGTGAATATCATCATGAAAGATTTGATTAAGCTGATGGA
TCCTAAATATATTGAGGTGACGGGCATTTTTACTCCGCGCGGTGGTATTTCCATTTATCCGTATTGTAATTACGGCCGTC
CGGGTACTAAGTATGAGGAATTGGCGGAGTATCGGATGCGTAATCACGATTTATAG

Upstream 100 bases:

>100_bases
ACAGTTTCATCACTTTAGTGAAACGATGTACTTGGCAGGGAGAGAATTTCTATTCCAAGGTATGAAAGAGAAAACATGAA
TATGAAAAAAACAGATTTTA

Downstream 100 bases:

>100_bases
ATGTATCTCTTGGAATGATTAGATAGATTGTGGACTCAAATATTTGCTGGTTTTGAAAATTTGTTCTATTTTTGCACTCC
GAAAAAAGGAGAGGTGCTCG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MERKDELTLLGSKTEYRQDYAPEVLESFVNKHPGNDYWVRFNCPEFTSLCPITGQPDFAEIRISYLPDVKMVESKSLKLY
LFSFRNHGDFHEDCVNIIMKDLIKLMDPKYIEVTGIFTPRGGISIYPYCNYGRPGTKYEELAEYRMRNHDL

Sequences:

>Translated_151_residues
MERKDELTLLGSKTEYRQDYAPEVLESFVNKHPGNDYWVRFNCPEFTSLCPITGQPDFAEIRISYLPDVKMVESKSLKLY
LFSFRNHGDFHEDCVNIIMKDLIKLMDPKYIEVTGIFTPRGGISIYPYCNYGRPGTKYEELAEYRMRNHDL
>Mature_151_residues
MERKDELTLLGSKTEYRQDYAPEVLESFVNKHPGNDYWVRFNCPEFTSLCPITGQPDFAEIRISYLPDVKMVESKSLKLY
LFSFRNHGDFHEDCVNIIMKDLIKLMDPKYIEVTGIFTPRGGISIYPYCNYGRPGTKYEELAEYRMRNHDL

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=81, Percent_Identity=39.5061728395062, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016856
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 17764; Mature: 17764

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKDELTLLGSKTEYRQDYAPEVLESFVNKHPGNDYWVRFNCPEFTSLCPITGQPDFAE
CCCCCCEEEECCCCHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHE
IRISYLPDVKMVESKSLKLYLFSFRNHGDFHEDCVNIIMKDLIKLMDPKYIEVTGIFTPR
EEEECCCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
GGISIYPYCNYGRPGTKYEELAEYRMRNHDL
CCEEEEEEECCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MERKDELTLLGSKTEYRQDYAPEVLESFVNKHPGNDYWVRFNCPEFTSLCPITGQPDFAE
CCCCCCEEEECCCCHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHE
IRISYLPDVKMVESKSLKLYLFSFRNHGDFHEDCVNIIMKDLIKLMDPKYIEVTGIFTPR
EEEECCCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
GGISIYPYCNYGRPGTKYEELAEYRMRNHDL
CCEEEEEEECCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA