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The map label for this gene is purA

Identifier: 150004563

GI number: 150004563

Start: 2610121

End: 2611392

Strand: Direct

Name: purA

Synonym: BVU_2018

Alternate gene names: 150004563

Gene position: 2610121-2611392 (Clockwise)

Preceding gene: 150004562

Following gene: 150004564

Centisome position: 50.55

GC content: 43.32

Gene sequence:

>1272_bases
ATGAAAGTAGATGTCTTGTTAGGTTTGCAATGGGGTGATGAAGGCAAAGGAAAGGTTGTCGATGTTCTGACTCCCCGTTA
TGATGTGGTAGCTCGTTTCCAGGGAGGTCCGAATGCAGGTCATACACTGGAATTCGAAGGCCAGAAATATGTGCTCCGTT
CCATTCCTTCCGGAATATTTCAAGGAAATAAAGTAAACATTATTGGTAATGGTGTGGTACTGGATCCGGCTTTGTTCAAG
GCTGAGGCTGAAGCTTTGGAGGCTAGCGGACACCCCCTGAAAGAACGTTTGCACATTTCAAAGAAAGCTCATTTGATTTT
GCCTACTCACCGCATTCTGGATGCTGCTTATGAAGCTGCTAAAGGAGATGCCAAAGTGGGAACTACCGGAAAAGGTATAG
GTCCTACTTATACTGATAAAGTGAGTCGTAACGGTGTACGTGTAGGTGATATCCTTCATAATTTTGAAGAAGTGTATGGA
AAGGCAAAGGCACGCCACGAACAGATCTTGAAAAGTTTGAATTATGAATATGATATAACCGAACTGGAAAAACAATGGTT
GGAGGGTATCGAATACCTGAAACAGTTCCATTTGGTGGACAGTGAACACGAAATCAATAATCTTCTGAAGTCGGGTAAGA
GTGTGCTTTGTGAAGGTGCGCAGGGAACGATGCTTGATGTTGATTTCGGTTCTTATCCTTTTGTCACTTCTTCAAATACT
ATTTGTGCCGGTGCTTGTACCGGTTTAGGTATCGGTCCTAATAAAATTGGTAATGTATACGGTATCATGAAAGCGTATTG
TACCCGTGTGGGGGCAGGTCCTTTCCCTACCGAACTGTTTGATGAAACTGGTAAGAAAATCCGCGATTTAGGTCATGAAT
ATGGTGCGGTGACAGGTCGTGAACGTCGTTGTGGCTGGATTGATCTTGTAGCTTTGAAGTATTCTATCATGGTAAACGGG
GTGACTCAGTTAATTATGATGAAGAGCGATGTGTTGGATGATTTCGAAACCATCAAAGCATGTGTGGCTTATAAAGTAAA
TGGTGAGGAAATTGATTACTTCCCTTATGATATTAGTGAGGGGCTGGAACCTGTATATGCGGAGCTGCCCGGATGGAAGA
CTGATATGACCAAGATGACCAGTGAGGATGAATTCCCGGAAGAATTCAATGCGTATGTCACTTTCTTGGAAGAACAGCTG
GAAACCCCGATCAAGATTGTTTCGGTAGGTCCTGATCGTGGTCAGACTATTGAACGCTATACGGAAGAATAA

Upstream 100 bases:

>100_bases
CATGCCTCGCACTATTCCTTATATATCTATGGTGTATGCAGCAAGTGTACTTGGGCCAAGCGTAGGAAGAAAAAAGATAA
GTAACATAAAACGAAGAAAC

Downstream 100 bases:

>100_bases
TAGAGAAGAATCTTATAAGATAAAAAAGAGATACTGTTTTCCTTTGGAGTCAGTTTCTCTTTTTTTTTGTATACATCTAT
CTGTTTCTATGTATATTTGA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKVDVLLGLQWGDEGKGKVVDVLTPRYDVVARFQGGPNAGHTLEFEGQKYVLRSIPSGIFQGNKVNIIGNGVVLDPALFK
AEAEALEASGHPLKERLHISKKAHLILPTHRILDAAYEAAKGDAKVGTTGKGIGPTYTDKVSRNGVRVGDILHNFEEVYG
KAKARHEQILKSLNYEYDITELEKQWLEGIEYLKQFHLVDSEHEINNLLKSGKSVLCEGAQGTMLDVDFGSYPFVTSSNT
ICAGACTGLGIGPNKIGNVYGIMKAYCTRVGAGPFPTELFDETGKKIRDLGHEYGAVTGRERRCGWIDLVALKYSIMVNG
VTQLIMMKSDVLDDFETIKACVAYKVNGEEIDYFPYDISEGLEPVYAELPGWKTDMTKMTSEDEFPEEFNAYVTFLEEQL
ETPIKIVSVGPDRGQTIERYTEE

Sequences:

>Translated_423_residues
MKVDVLLGLQWGDEGKGKVVDVLTPRYDVVARFQGGPNAGHTLEFEGQKYVLRSIPSGIFQGNKVNIIGNGVVLDPALFK
AEAEALEASGHPLKERLHISKKAHLILPTHRILDAAYEAAKGDAKVGTTGKGIGPTYTDKVSRNGVRVGDILHNFEEVYG
KAKARHEQILKSLNYEYDITELEKQWLEGIEYLKQFHLVDSEHEINNLLKSGKSVLCEGAQGTMLDVDFGSYPFVTSSNT
ICAGACTGLGIGPNKIGNVYGIMKAYCTRVGAGPFPTELFDETGKKIRDLGHEYGAVTGRERRCGWIDLVALKYSIMVNG
VTQLIMMKSDVLDDFETIKACVAYKVNGEEIDYFPYDISEGLEPVYAELPGWKTDMTKMTSEDEFPEEFNAYVTFLEEQL
ETPIKIVSVGPDRGQTIERYTEE
>Mature_423_residues
MKVDVLLGLQWGDEGKGKVVDVLTPRYDVVARFQGGPNAGHTLEFEGQKYVLRSIPSGIFQGNKVNIIGNGVVLDPALFK
AEAEALEASGHPLKERLHISKKAHLILPTHRILDAAYEAAKGDAKVGTTGKGIGPTYTDKVSRNGVRVGDILHNFEEVYG
KAKARHEQILKSLNYEYDITELEKQWLEGIEYLKQFHLVDSEHEINNLLKSGKSVLCEGAQGTMLDVDFGSYPFVTSSNT
ICAGACTGLGIGPNKIGNVYGIMKAYCTRVGAGPFPTELFDETGKKIRDLGHEYGAVTGRERRCGWIDLVALKYSIMVNG
VTQLIMMKSDVLDDFETIKACVAYKVNGEEIDYFPYDISEGLEPVYAELPGWKTDMTKMTSEDEFPEEFNAYVTFLEEQL
ETPIKIVSVGPDRGQTIERYTEE

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=430, Percent_Identity=43.7209302325581, Blast_Score=338, Evalue=4e-93,
Organism=Homo sapiens, GI34577063, Length=431, Percent_Identity=43.6194895591647, Blast_Score=324, Evalue=9e-89,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=42.2110552763819, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1790620, Length=433, Percent_Identity=45.2655889145497, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=44.5221445221445, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=44.5221445221445, Blast_Score=343, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=43.7209302325581, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI21356033, Length=429, Percent_Identity=44.5221445221445, Blast_Score=346, Evalue=2e-95,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_BACV8 (A6L1X1)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299307.1
- ProteinModelPortal:   A6L1X1
- SMR:   A6L1X1
- STRING:   A6L1X1
- GeneID:   5302984
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_2018
- NMPDR:   fig|435590.6.peg.1896
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46821; Mature: 46821

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 129-129 BINDING 143-143 BINDING 221-221 BINDING 236-236 BINDING 300-300 BINDING 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDVLLGLQWGDEGKGKVVDVLTPRYDVVARFQGGPNAGHTLEFEGQKYVLRSIPSGIF
CEEEEEEEEEECCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEECCCHHHHHHCCHHHC
QGNKVNIIGNGVVLDPALFKAEAEALEASGHPLKERLHISKKAHLILPTHRILDAAYEAA
CCCEEEEEECCEEECHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECHHHHHHHHHHHC
KGDAKVGTTGKGIGPTYTDKVSRNGVRVGDILHNFEEVYGKAKARHEQILKSLNYEYDIT
CCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ELEKQWLEGIEYLKQFHLVDSEHEINNLLKSGKSVLCEGAQGTMLDVDFGSYPFVTSSNT
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCEEECCCC
ICAGACTGLGIGPNKIGNVYGIMKAYCTRVGAGPFPTELFDETGKKIRDLGHEYGAVTGR
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
ERRCGWIDLVALKYSIMVNGVTQLIMMKSDVLDDFETIKACVAYKVNGEEIDYFPYDISE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCHHH
GLEPVYAELPGWKTDMTKMTSEDEFPEEFNAYVTFLEEQLETPIKIVSVGPDRGQTIERY
CCCHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
TEE
CCC
>Mature Secondary Structure
MKVDVLLGLQWGDEGKGKVVDVLTPRYDVVARFQGGPNAGHTLEFEGQKYVLRSIPSGIF
CEEEEEEEEEECCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEECCCHHHHHHCCHHHC
QGNKVNIIGNGVVLDPALFKAEAEALEASGHPLKERLHISKKAHLILPTHRILDAAYEAA
CCCEEEEEECCEEECHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECHHHHHHHHHHHC
KGDAKVGTTGKGIGPTYTDKVSRNGVRVGDILHNFEEVYGKAKARHEQILKSLNYEYDIT
CCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ELEKQWLEGIEYLKQFHLVDSEHEINNLLKSGKSVLCEGAQGTMLDVDFGSYPFVTSSNT
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCEEECCCC
ICAGACTGLGIGPNKIGNVYGIMKAYCTRVGAGPFPTELFDETGKKIRDLGHEYGAVTGR
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
ERRCGWIDLVALKYSIMVNGVTQLIMMKSDVLDDFETIKACVAYKVNGEEIDYFPYDISE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCHHH
GLEPVYAELPGWKTDMTKMTSEDEFPEEFNAYVTFLEEQLETPIKIVSVGPDRGQTIERY
CCCHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
TEE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA