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The map label for this gene is pycB [H]

Identifier: 150004525

GI number: 150004525

Start: 2554395

End: 2556257

Strand: Direct

Name: pycB [H]

Synonym: BVU_1975

Alternate gene names: 150004525

Gene position: 2554395-2556257 (Clockwise)

Preceding gene: 150004524

Following gene: 150004526

Centisome position: 49.47

GC content: 45.25

Gene sequence:

>1863_bases
ATGGAAAGAGAAGTAAAATTTAGTCTGGTCTTCCGCGATATGTGGCAGAGTGCGGGTAAGTACGTGCCTCGTGTAGACCA
GTTGGTTAAGGTTGCCCCGGCTATTATTGAAATGGGCTGTTTCGCCCGTGTGGAAACAAATGGTGGTGGTTTTGAACAGG
TAAATTTATTGTTTGGTGAAAATCCGAATAGAGCTGTGCGCGAGTGGACGAAACCCTTCCACGAAGCAGGCATCCAGACT
CATATGCTGGACCGTGCGCTGAACGGTCTCCGCATGAGTCCCGTCCCGGCAGATGTCCGTAAATTATTCTATAAGGTTAA
ACATGCGCAAGGAACAGATATTACCCGTACTTTCTGCGGATTGAATGATGTCCGCAATATTATCCCTTCTATCGGCTATG
CTCACGAAGCCGGAATGATTTCACAATGTTCGCTCTGTATCACTTTTTCTCCGATACATACTGTGGAATATTATGTGAAT
ATGGCCAGGCAGCTTATAGAAGCCGGAGCCGATGAAATCTGTATTAAAGATATGGCCGGCATCGGACGTCCCGTTTCATT
GGGAAAGATTGTTGCCGGAATCAAGAAGATTAAAAATATTCCTATACAGTATCACTCTCATGCGGGGCCCGGATTCAATA
TGGCTTCTATTTTGGAAGTATGCCAGGCTGGTTGCGACTATATTGATGTAGGTATGGAACCATTGTCATGGGGAACCGGC
CATGCTGATTTGATTAGTGTGCAAGCCATGTTGAAAGATGCCGGATTTAAAGTGCCCGAAATTAATATGGAAGCGTATAT
GAAAGTGCGTTCCATGATTCAGGAGTTTATGGATGATTTCCTTGGTTTATATATCAGTCCCAAAAACCGTCTGATGAATT
CTTTACTGATTGCTCCGGGATTGCCGGGCGGCATGATGGGAAGTTTGATGGCCGATCTTGAAACGAATCTGGAATCTATC
AACAAGTACAAGGCAAAACGTAACCTGCCGTTTATGACTCAGGATGAATTGTTGATCAAGCTGTTCAATGAAGTTGCTTA
CGTATGGCCGCGTGTGGGTTATCCTCCTTTGGTTACTCCGTTCAGCCAGTATGTGAAGAACCTTGCCATGATGAATGTAA
TGGCTATGGAGAAAGGTAAGGAACGTTGGGGAATGATTGCCGATGATATTTGGGATATGATTTTGGGTAAAGCGGGACGT
TTACCGGGTAAACTGGCCCCTGAAATTATTGAAAAGGCAGAACGTGAAGGCCGCAAGTTCTTTGATGGCAATCCGCAGGA
CAATTATCCCGACCAGTTGGAAAAATATCGCAAGATGATGCTTGAAAAACAATGGGATAAAGGACAGGATGACGAAGAAC
TGTTTGAGTATGCCATGCATCCGGCCCAGTATGAAGCTTATAAGAGCGGGAAGGCAAAAGAAGACTTCTTGGCAGATGTG
AAGAAACGTCGTGAGGAGAAAGCGAATGCCACTACTCCTGTCGAAGCTGAAAACAAGACCAAGGTATTGACGGTGGATGT
GAACGGGCAACCTTATCGCGTTACAGTGGCATATGGTGCTGTAGATCCTGCTACGCTGACAGCTCCTTCGGGTGCAGTTC
CTGCACAAGCTGCTCCTGCTCCAGTAGGGGAAGGGAAAGATGTGCTTTCTCCGTTGGAAGGTAAATTCTTCTTGGTAAAG
AATGCCCAGGAAACTCCGAAGAAGGTGGGTGAAAAGGTTAATAAAGGTGATGTGATTTGTTACGTCGAAGCAATGAAGAC
CTATAATGCGATTCGTGCGGAATATGACGGTACTATAACTGCTATTTGCGCTAATTCGGGTGACACGGTTTCAGAGGATG
ACGTATTAATGAAAATAGTATAA

Upstream 100 bases:

>100_bases
TGCTATTAAGGCGGCAGTTGAGATTTTGACAGCAGGTAAAGGTCAGGTGATAAAGATTGAGAAGTTATAATCCAAAAAAA
GAAAAACACATTTATCAAAA

Downstream 100 bases:

>100_bases
TGGAAGATATATTTGAAAGACTTTATGATATGACTGCTTTCAGCAATATTATTGCTGAACCGCAATTCTTGATAATGTAT
GCCATTGCGTTCATCTTACT

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MEREVKFSLVFRDMWQSAGKYVPRVDQLVKVAPAIIEMGCFARVETNGGGFEQVNLLFGENPNRAVREWTKPFHEAGIQT
HMLDRALNGLRMSPVPADVRKLFYKVKHAQGTDITRTFCGLNDVRNIIPSIGYAHEAGMISQCSLCITFSPIHTVEYYVN
MARQLIEAGADEICIKDMAGIGRPVSLGKIVAGIKKIKNIPIQYHSHAGPGFNMASILEVCQAGCDYIDVGMEPLSWGTG
HADLISVQAMLKDAGFKVPEINMEAYMKVRSMIQEFMDDFLGLYISPKNRLMNSLLIAPGLPGGMMGSLMADLETNLESI
NKYKAKRNLPFMTQDELLIKLFNEVAYVWPRVGYPPLVTPFSQYVKNLAMMNVMAMEKGKERWGMIADDIWDMILGKAGR
LPGKLAPEIIEKAEREGRKFFDGNPQDNYPDQLEKYRKMMLEKQWDKGQDDEELFEYAMHPAQYEAYKSGKAKEDFLADV
KKRREEKANATTPVEAENKTKVLTVDVNGQPYRVTVAYGAVDPATLTAPSGAVPAQAAPAPVGEGKDVLSPLEGKFFLVK
NAQETPKKVGEKVNKGDVICYVEAMKTYNAIRAEYDGTITAICANSGDTVSEDDVLMKIV

Sequences:

>Translated_620_residues
MEREVKFSLVFRDMWQSAGKYVPRVDQLVKVAPAIIEMGCFARVETNGGGFEQVNLLFGENPNRAVREWTKPFHEAGIQT
HMLDRALNGLRMSPVPADVRKLFYKVKHAQGTDITRTFCGLNDVRNIIPSIGYAHEAGMISQCSLCITFSPIHTVEYYVN
MARQLIEAGADEICIKDMAGIGRPVSLGKIVAGIKKIKNIPIQYHSHAGPGFNMASILEVCQAGCDYIDVGMEPLSWGTG
HADLISVQAMLKDAGFKVPEINMEAYMKVRSMIQEFMDDFLGLYISPKNRLMNSLLIAPGLPGGMMGSLMADLETNLESI
NKYKAKRNLPFMTQDELLIKLFNEVAYVWPRVGYPPLVTPFSQYVKNLAMMNVMAMEKGKERWGMIADDIWDMILGKAGR
LPGKLAPEIIEKAEREGRKFFDGNPQDNYPDQLEKYRKMMLEKQWDKGQDDEELFEYAMHPAQYEAYKSGKAKEDFLADV
KKRREEKANATTPVEAENKTKVLTVDVNGQPYRVTVAYGAVDPATLTAPSGAVPAQAAPAPVGEGKDVLSPLEGKFFLVK
NAQETPKKVGEKVNKGDVICYVEAMKTYNAIRAEYDGTITAICANSGDTVSEDDVLMKIV
>Mature_620_residues
MEREVKFSLVFRDMWQSAGKYVPRVDQLVKVAPAIIEMGCFARVETNGGGFEQVNLLFGENPNRAVREWTKPFHEAGIQT
HMLDRALNGLRMSPVPADVRKLFYKVKHAQGTDITRTFCGLNDVRNIIPSIGYAHEAGMISQCSLCITFSPIHTVEYYVN
MARQLIEAGADEICIKDMAGIGRPVSLGKIVAGIKKIKNIPIQYHSHAGPGFNMASILEVCQAGCDYIDVGMEPLSWGTG
HADLISVQAMLKDAGFKVPEINMEAYMKVRSMIQEFMDDFLGLYISPKNRLMNSLLIAPGLPGGMMGSLMADLETNLESI
NKYKAKRNLPFMTQDELLIKLFNEVAYVWPRVGYPPLVTPFSQYVKNLAMMNVMAMEKGKERWGMIADDIWDMILGKAGR
LPGKLAPEIIEKAEREGRKFFDGNPQDNYPDQLEKYRKMMLEKQWDKGQDDEELFEYAMHPAQYEAYKSGKAKEDFLADV
KKRREEKANATTPVEAENKTKVLTVDVNGQPYRVTVAYGAVDPATLTAPSGAVPAQAAPAPVGEGKDVLSPLEGKFFLVK
NAQETPKKVGEKVNKGDVICYVEAMKTYNAIRAEYDGTITAICANSGDTVSEDDVLMKIV

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=647, Percent_Identity=23.338485316847, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI106049295, Length=647, Percent_Identity=23.338485316847, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI106049292, Length=647, Percent_Identity=23.338485316847, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17562816, Length=654, Percent_Identity=23.5474006116208, Blast_Score=108, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6319695, Length=648, Percent_Identity=22.2222222222222, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321376, Length=648, Percent_Identity=22.2222222222222, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI281363050, Length=252, Percent_Identity=31.3492063492063, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24652224, Length=252, Percent_Identity=31.3492063492063, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24652222, Length=252, Percent_Identity=31.3492063492063, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24652220, Length=252, Percent_Identity=31.3492063492063, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24652218, Length=252, Percent_Identity=31.3492063492063, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24652212, Length=251, Percent_Identity=31.4741035856574, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24652210, Length=251, Percent_Identity=31.4741035856574, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24652214, Length=251, Percent_Identity=31.4741035856574, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI19921944, Length=251, Percent_Identity=31.4741035856574, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24652216, Length=251, Percent_Identity=31.4741035856574, Blast_Score=102, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 69023; Mature: 69023

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEREVKFSLVFRDMWQSAGKYVPRVDQLVKVAPAIIEMGCFARVETNGGGFEQVNLLFGE
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECC
NPNRAVREWTKPFHEAGIQTHMLDRALNGLRMSPVPADVRKLFYKVKHAQGTDITRTFCG
CCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHC
LNDVRNIIPSIGYAHEAGMISQCSLCITFSPIHTVEYYVNMARQLIEAGADEICIKDMAG
HHHHHHHHHCCCCHHCCCCHHHCEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
IGRPVSLGKIVAGIKKIKNIPIQYHSHAGPGFNMASILEVCQAGCDYIDVGMEPLSWGTG
CCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCC
HADLISVQAMLKDAGFKVPEINMEAYMKVRSMIQEFMDDFLGLYISPKNRLMNSLLIAPG
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHCCC
LPGGMMGSLMADLETNLESINKYKAKRNLPFMTQDELLIKLFNEVAYVWPRVGYPPLVTP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCH
FSQYVKNLAMMNVMAMEKGKERWGMIADDIWDMILGKAGRLPGKLAPEIIEKAEREGRKF
HHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
FDGNPQDNYPDQLEKYRKMMLEKQWDKGQDDEELFEYAMHPAQYEAYKSGKAKEDFLADV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHH
KKRREEKANATTPVEAENKTKVLTVDVNGQPYRVTVAYGAVDPATLTAPSGAVPAQAAPA
HHHHHHHCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCEECCCCCCCCCCCCCC
PVGEGKDVLSPLEGKFFLVKNAQETPKKVGEKVNKGDVICYVEAMKTYNAIRAEYDGTIT
CCCCCHHHHCCCCCCEEEEECCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCEEE
AICANSGDTVSEDDVLMKIV
EEEECCCCCCCCCCEEEEEC
>Mature Secondary Structure
MEREVKFSLVFRDMWQSAGKYVPRVDQLVKVAPAIIEMGCFARVETNGGGFEQVNLLFGE
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECC
NPNRAVREWTKPFHEAGIQTHMLDRALNGLRMSPVPADVRKLFYKVKHAQGTDITRTFCG
CCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHC
LNDVRNIIPSIGYAHEAGMISQCSLCITFSPIHTVEYYVNMARQLIEAGADEICIKDMAG
HHHHHHHHHCCCCHHCCCCHHHCEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
IGRPVSLGKIVAGIKKIKNIPIQYHSHAGPGFNMASILEVCQAGCDYIDVGMEPLSWGTG
CCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCC
HADLISVQAMLKDAGFKVPEINMEAYMKVRSMIQEFMDDFLGLYISPKNRLMNSLLIAPG
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHCCC
LPGGMMGSLMADLETNLESINKYKAKRNLPFMTQDELLIKLFNEVAYVWPRVGYPPLVTP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCH
FSQYVKNLAMMNVMAMEKGKERWGMIADDIWDMILGKAGRLPGKLAPEIIEKAEREGRKF
HHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
FDGNPQDNYPDQLEKYRKMMLEKQWDKGQDDEELFEYAMHPAQYEAYKSGKAKEDFLADV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHH
KKRREEKANATTPVEAENKTKVLTVDVNGQPYRVTVAYGAVDPATLTAPSGAVPAQAAPA
HHHHHHHCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCEECCCCCCCCCCCCCC
PVGEGKDVLSPLEGKFFLVKNAQETPKKVGEKVNKGDVICYVEAMKTYNAIRAEYDGTIT
CCCCCHHHHCCCCCCEEEEECCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCEEE
AICANSGDTVSEDDVLMKIV
EEEECCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]