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The map label for this gene is acnA [H]

Identifier: 150004509

GI number: 150004509

Start: 2538623

End: 2540878

Strand: Direct

Name: acnA [H]

Synonym: BVU_1959

Alternate gene names: 150004509

Gene position: 2538623-2540878 (Clockwise)

Preceding gene: 150004507

Following gene: 150004510

Centisome position: 49.17

GC content: 47.34

Gene sequence:

>2256_bases
ATGGTAAAAGGTATGGTATATGACTTGGACATGTTAAAGGCCTTTTATGCTTCTTTCGAAAAGAAGATAGGTCGGGTAAG
AGCCGTGTTGCAGCGTCCGCTGACATTGGCTGAGAAAATTCTGTATACACATCTTTATGATGATGCTCAACTGAAGAATT
ATGAACGTGGCGAGGACTATGTGAATTTCCGTCCCGACCGCGTGGCTATGCAAGACGCGACGGCGCAAATGGCATTGTTG
CAATTTATCAATGCAGGAAAGGATTCCGCTGCCGTACCTTCTACTGTGCATTGTGACCATTTGATTCAGGCATATAAAGG
AGCGGGCCCGGATATTGCAACGGCTACGGAAACCAACCGTGAGGTATACGATTTCTTACGAGATGTTTCTTCGCGTTTCG
GTATCGGGTTTTGGAAACCGGGAGCAGGAATCATTCATCAGGTGGTACTCGAAAACTATGCTTTTCCCGGGGGAATGATG
GTGGGGACGGATTCTCATACCCCCAATGCCGGAGGATTGGGGATGGTGGCAATCGGAGTCGGTGGTGCCGATGCTGTGGA
TGTAATGACCGGTATGGAATGGGAGTTAAAGATGCCGAAACTGATTGGAGTTCATTTGACCGGAAGCTTGAATGGATGGG
CTTCGCCAAAGGATGTGATTCTGAAATTGGCCGGTATTCTGACCGTGAAAGGTGGAACGAATGCCATTATAGAATATTTT
GGAGAAGGGACAGCTTCACTTTCGGCAACCGGTAAGGCTACTATCTGCAATATGGGTGCAGAGGTGGGGGCTACCACATC
CTTGTTCCCCTATGACGACCGGATGGCTGTGTATCTGAAAGCTACCGGAAGAGAGTCTGTAGCAGAGATGGCCGGAAAAG
TGGCAAATGATTTACGTGCCGATGCTGAGGTTGTTGCCAATCCTTCTGCTTTCTACGACCGTGTGATTGAAATTAATTTG
TCCGAGTTGGAACCTTATATCAACGGGCCGTTTACACCGGATGCCGCAACACCTATTTCAGAGTTTGCCGAAAAGGTATT
GGTTAATGGTTATCCGCGTAAGATGGAAGTCGGGCTGATAGGTTCATGTACCAATTCATCTTATCAGGATTTAAGTCGGG
CTGCTTCCATTGCCCGTCAGGTAGCTGAAAAACATTTGGCTGTGGCTGCTCCTTTGATTGTCAATCCGGGTTCTGAACAG
ATTCGAGCTACCGCCGAGCGTGACGGGATGATTGATGCTTTCCAAAAGATTGGGGCTACTATTATGGCGAATGCTTGCGG
TCCGTGCATCGGACAGTGGAAACGCCATACCGATGATCCTGTCCGCAAAAACTCGATTGTCACTTCTTTCAACCGTAATT
TTGCCAAACGTGCCGATGGTAATCCGAATACACATGCATTTGTGGCTTCTCCTGAATTGGTATTGGCTCTGACTATTGCC
GGTGATTTATGTTTCAATCCGTTGAAAGATACGTTGATAAATCAAGAAGGCGAGAAGGTGAAATTGAGCGTTCCCGAGGG
AGATGAATTGCCGTCGGCAGGTTTCACTCAAGGTAATCCCGGTTATTTGGCTCCTGCCGGTGCACAGGTAGAAATTAAGG
TGAATCCCGAATCACAGCGTTTGCAGTTATTGGCTCCGTTTCCGGCATGGGATGGAAAGGATTTTACGGATATGCCTTTA
CTGATTAAGGCTCAAGGTAAATGTACCACAGACCATATTTCGATGGCAGGCCCGTGGCTGCGTTTCCGTGGTCATCTGGA
AAATATTTCGGATAATATGCTGATGGGGGCGGTGAACGCTTTCAATGGTGAAACGAACAAGGTGTGGAACCGATTGACAA
ATACCTATGAGAGTGTATCGGGCGCGGCTAAGCAGTATAAAGCGCAAGGCATCGGGTCTATGGTAGTTGCGGAGGAAAAT
TATGGAGAAGGTTCCAGTCGTGAACATGCCGCTATGGAGCCTCGTTTCCTGAATGTAAAGGTTATTCTGGCGAAGAGTTT
TGCCCGCATCCATGAAACGAATCTGAAGAAGCAGGGAATGTTGGCGGTGACTTTTATAGACAAAGCGGACTATGACAAGA
TTCAAGAACATGATTTGATTACCGTCAGCGGATTGGCGGACTTTGCTCCCGGAAGAAATCTGACTGTGACTTTACACCAT
GAAGATGGCACTCAGGATGGTTTTGAAGTACAGCATACTTATAATGAACAGCAGATAGGTTGGTTCCGTGCCGGCTCTGC
TTTAAATGCAAGATAA

Upstream 100 bases:

>100_bases
ATGTTTTATTTTGTTAAACGTATTATAGATTACACAAGAATTCAAATATTATTCCTATATTGCCAAAAATAAAATAGATT
TAAAACTGTAATAATTATAA

Downstream 100 bases:

>100_bases
AAGATGAAAAAGACAGTACCTTATATTACAGGAGACGGAGTGGGAGCAGAAATTACTCCCGCTATGCAAGCCATAGTGAA
TGCGGCCGTGAAGAAGGCGT

Product: aconitate hydratase

Products: NA

Alternate protein names: Aconitase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 751; Mature: 751

Protein sequence:

>751_residues
MVKGMVYDLDMLKAFYASFEKKIGRVRAVLQRPLTLAEKILYTHLYDDAQLKNYERGEDYVNFRPDRVAMQDATAQMALL
QFINAGKDSAAVPSTVHCDHLIQAYKGAGPDIATATETNREVYDFLRDVSSRFGIGFWKPGAGIIHQVVLENYAFPGGMM
VGTDSHTPNAGGLGMVAIGVGGADAVDVMTGMEWELKMPKLIGVHLTGSLNGWASPKDVILKLAGILTVKGGTNAIIEYF
GEGTASLSATGKATICNMGAEVGATTSLFPYDDRMAVYLKATGRESVAEMAGKVANDLRADAEVVANPSAFYDRVIEINL
SELEPYINGPFTPDAATPISEFAEKVLVNGYPRKMEVGLIGSCTNSSYQDLSRAASIARQVAEKHLAVAAPLIVNPGSEQ
IRATAERDGMIDAFQKIGATIMANACGPCIGQWKRHTDDPVRKNSIVTSFNRNFAKRADGNPNTHAFVASPELVLALTIA
GDLCFNPLKDTLINQEGEKVKLSVPEGDELPSAGFTQGNPGYLAPAGAQVEIKVNPESQRLQLLAPFPAWDGKDFTDMPL
LIKAQGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNGETNKVWNRLTNTYESVSGAAKQYKAQGIGSMVVAEEN
YGEGSSREHAAMEPRFLNVKVILAKSFARIHETNLKKQGMLAVTFIDKADYDKIQEHDLITVSGLADFAPGRNLTVTLHH
EDGTQDGFEVQHTYNEQQIGWFRAGSALNAR

Sequences:

>Translated_751_residues
MVKGMVYDLDMLKAFYASFEKKIGRVRAVLQRPLTLAEKILYTHLYDDAQLKNYERGEDYVNFRPDRVAMQDATAQMALL
QFINAGKDSAAVPSTVHCDHLIQAYKGAGPDIATATETNREVYDFLRDVSSRFGIGFWKPGAGIIHQVVLENYAFPGGMM
VGTDSHTPNAGGLGMVAIGVGGADAVDVMTGMEWELKMPKLIGVHLTGSLNGWASPKDVILKLAGILTVKGGTNAIIEYF
GEGTASLSATGKATICNMGAEVGATTSLFPYDDRMAVYLKATGRESVAEMAGKVANDLRADAEVVANPSAFYDRVIEINL
SELEPYINGPFTPDAATPISEFAEKVLVNGYPRKMEVGLIGSCTNSSYQDLSRAASIARQVAEKHLAVAAPLIVNPGSEQ
IRATAERDGMIDAFQKIGATIMANACGPCIGQWKRHTDDPVRKNSIVTSFNRNFAKRADGNPNTHAFVASPELVLALTIA
GDLCFNPLKDTLINQEGEKVKLSVPEGDELPSAGFTQGNPGYLAPAGAQVEIKVNPESQRLQLLAPFPAWDGKDFTDMPL
LIKAQGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNGETNKVWNRLTNTYESVSGAAKQYKAQGIGSMVVAEEN
YGEGSSREHAAMEPRFLNVKVILAKSFARIHETNLKKQGMLAVTFIDKADYDKIQEHDLITVSGLADFAPGRNLTVTLHH
EDGTQDGFEVQHTYNEQQIGWFRAGSALNAR
>Mature_751_residues
MVKGMVYDLDMLKAFYASFEKKIGRVRAVLQRPLTLAEKILYTHLYDDAQLKNYERGEDYVNFRPDRVAMQDATAQMALL
QFINAGKDSAAVPSTVHCDHLIQAYKGAGPDIATATETNREVYDFLRDVSSRFGIGFWKPGAGIIHQVVLENYAFPGGMM
VGTDSHTPNAGGLGMVAIGVGGADAVDVMTGMEWELKMPKLIGVHLTGSLNGWASPKDVILKLAGILTVKGGTNAIIEYF
GEGTASLSATGKATICNMGAEVGATTSLFPYDDRMAVYLKATGRESVAEMAGKVANDLRADAEVVANPSAFYDRVIEINL
SELEPYINGPFTPDAATPISEFAEKVLVNGYPRKMEVGLIGSCTNSSYQDLSRAASIARQVAEKHLAVAAPLIVNPGSEQ
IRATAERDGMIDAFQKIGATIMANACGPCIGQWKRHTDDPVRKNSIVTSFNRNFAKRADGNPNTHAFVASPELVLALTIA
GDLCFNPLKDTLINQEGEKVKLSVPEGDELPSAGFTQGNPGYLAPAGAQVEIKVNPESQRLQLLAPFPAWDGKDFTDMPL
LIKAQGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNGETNKVWNRLTNTYESVSGAAKQYKAQGIGSMVVAEEN
YGEGSSREHAAMEPRFLNVKVILAKSFARIHETNLKKQGMLAVTFIDKADYDKIQEHDLITVSGLADFAPGRNLTVTLHH
EDGTQDGFEVQHTYNEQQIGWFRAGSALNAR

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=736, Percent_Identity=58.0163043478261, Blast_Score=870, Evalue=0.0,
Organism=Homo sapiens, GI8659555, Length=788, Percent_Identity=28.5532994923858, Blast_Score=203, Evalue=4e-52,
Organism=Homo sapiens, GI41352693, Length=420, Percent_Identity=29.5238095238095, Blast_Score=147, Evalue=5e-35,
Organism=Escherichia coli, GI87081781, Length=470, Percent_Identity=24.2553191489362, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1787531, Length=508, Percent_Identity=27.1653543307087, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1786259, Length=400, Percent_Identity=28, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25149337, Length=734, Percent_Identity=57.3569482288828, Blast_Score=862, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32564738, Length=680, Percent_Identity=58.0882352941177, Blast_Score=812, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25149342, Length=570, Percent_Identity=56.3157894736842, Blast_Score=667, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17568399, Length=826, Percent_Identity=27.1186440677966, Blast_Score=213, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6323335, Length=726, Percent_Identity=58.8154269972452, Blast_Score=881, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322261, Length=754, Percent_Identity=53.183023872679, Blast_Score=792, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320440, Length=690, Percent_Identity=25.5072463768116, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321429, Length=530, Percent_Identity=26.6037735849057, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI281365315, Length=736, Percent_Identity=58.4239130434783, Blast_Score=862, Evalue=0.0,
Organism=Drosophila melanogaster, GI17864292, Length=736, Percent_Identity=58.4239130434783, Blast_Score=862, Evalue=0.0,
Organism=Drosophila melanogaster, GI28571643, Length=736, Percent_Identity=57.2010869565217, Blast_Score=845, Evalue=0.0,
Organism=Drosophila melanogaster, GI161076999, Length=682, Percent_Identity=59.0909090909091, Blast_Score=806, Evalue=0.0,
Organism=Drosophila melanogaster, GI24645686, Length=834, Percent_Identity=28.0575539568345, Blast_Score=191, Evalue=2e-48,
Organism=Drosophila melanogaster, GI17137564, Length=831, Percent_Identity=26.3537906137184, Blast_Score=186, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR006249
- InterPro:   IPR015934
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00330 Aconitase; PF00694 Aconitase_C [H]

EC number: =4.2.1.3 [H]

Molecular weight: Translated: 81439; Mature: 81439

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKGMVYDLDMLKAFYASFEKKIGRVRAVLQRPLTLAEKILYTHLYDDAQLKNYERGEDY
CCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCC
VNFRPDRVAMQDATAQMALLQFINAGKDSAAVPSTVHCDHLIQAYKGAGPDIATATETNR
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCH
EVYDFLRDVSSRFGIGFWKPGAGIIHQVVLENYAFPGGMMVGTDSHTPNAGGLGMVAIGV
HHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEC
GGADAVDVMTGMEWELKMPKLIGVHLTGSLNGWASPKDVILKLAGILTVKGGTNAIIEYF
CCCCHHHHHCCCCEEEECCEEEEEEEEECCCCCCCHHHHHHHHHHEEEECCCCHHHHHHH
GEGTASLSATGKATICNMGAEVGATTSLFPYDDRMAVYLKATGRESVAEMAGKVANDLRA
CCCCCEEECCCCEEEEECCCCCCCCCEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHC
DAEVVANPSAFYDRVIEINLSELEPYINGPFTPDAATPISEFAEKVLVNGYPRKMEVGLI
CHHHHCCCHHHHHEEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
GSCTNSSYQDLSRAASIARQVAEKHLAVAAPLIVNPGSEQIRATAERDGMIDAFQKIGAT
ECCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEECCCHHHHHHHHHHCCHHHHHHHCCHH
IMANACGPCIGQWKRHTDDPVRKNSIVTSFNRNFAKRADGNPNTHAFVASPELVLALTIA
EEHHCCCHHHHHHHCCCCCCCHHCCEEEHHCCHHHHHCCCCCCCEEEEECCCEEEEEEEC
GDLCFNPLKDTLINQEGEKVKLSVPEGDELPSAGFTQGNPGYLAPAGAQVEIKVNPESQR
CHHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCE
LQLLAPFPAWDGKDFTDMPLLIKAQGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNA
EEEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHCCCCHHHHHHHHHCCCCCCEEEEHHH
FNGETNKVWNRLTNTYESVSGAAKQYKAQGIGSMVVAEENYGEGSSREHAAMEPRFLNVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEE
VILAKSFARIHETNLKKQGMLAVTFIDKADYDKIQEHDLITVSGLADFAPGRNLTVTLHH
EEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHCCCCEEEEECCHHCCCCCCEEEEEEE
EDGTQDGFEVQHTYNEQQIGWFRAGSALNAR
CCCCCCCEEEEECCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MVKGMVYDLDMLKAFYASFEKKIGRVRAVLQRPLTLAEKILYTHLYDDAQLKNYERGEDY
CCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCC
VNFRPDRVAMQDATAQMALLQFINAGKDSAAVPSTVHCDHLIQAYKGAGPDIATATETNR
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCH
EVYDFLRDVSSRFGIGFWKPGAGIIHQVVLENYAFPGGMMVGTDSHTPNAGGLGMVAIGV
HHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEC
GGADAVDVMTGMEWELKMPKLIGVHLTGSLNGWASPKDVILKLAGILTVKGGTNAIIEYF
CCCCHHHHHCCCCEEEECCEEEEEEEEECCCCCCCHHHHHHHHHHEEEECCCCHHHHHHH
GEGTASLSATGKATICNMGAEVGATTSLFPYDDRMAVYLKATGRESVAEMAGKVANDLRA
CCCCCEEECCCCEEEEECCCCCCCCCEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHC
DAEVVANPSAFYDRVIEINLSELEPYINGPFTPDAATPISEFAEKVLVNGYPRKMEVGLI
CHHHHCCCHHHHHEEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
GSCTNSSYQDLSRAASIARQVAEKHLAVAAPLIVNPGSEQIRATAERDGMIDAFQKIGAT
ECCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEECCCHHHHHHHHHHCCHHHHHHHCCHH
IMANACGPCIGQWKRHTDDPVRKNSIVTSFNRNFAKRADGNPNTHAFVASPELVLALTIA
EEHHCCCHHHHHHHCCCCCCCHHCCEEEHHCCHHHHHCCCCCCCEEEEECCCEEEEEEEC
GDLCFNPLKDTLINQEGEKVKLSVPEGDELPSAGFTQGNPGYLAPAGAQVEIKVNPESQR
CHHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCE
LQLLAPFPAWDGKDFTDMPLLIKAQGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNA
EEEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHCCCCHHHHHHHHHCCCCCCEEEEHHH
FNGETNKVWNRLTNTYESVSGAAKQYKAQGIGSMVVAEENYGEGSSREHAAMEPRFLNVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEE
VILAKSFARIHETNLKKQGMLAVTFIDKADYDKIQEHDLITVSGLADFAPGRNLTVTLHH
EEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHCCCCEEEEECCHHCCCCCCEEEEEEE
EDGTQDGFEVQHTYNEQQIGWFRAGSALNAR
CCCCCCCEEEEECCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA