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The map label for this gene is topB [H]

Identifier: 150004505

GI number: 150004505

Start: 2532500

End: 2534641

Strand: Reverse

Name: topB [H]

Synonym: BVU_1955

Alternate gene names: 150004505

Gene position: 2534641-2532500 (Counterclockwise)

Preceding gene: 150004508

Following gene: 150004503

Centisome position: 49.09

GC content: 44.63

Gene sequence:

>2142_bases
ATGATAGTTTGTATTGCCGAAAAACCTTCCGTAGCCCGTGACATAGCGGATGTACTGGGAGCAAAAACCAAAAAAGACGG
ATATATAGAAGGAAACGGATATCAGGTTACCTGGACATTCGGACACCTCTGTACTTTGAAGGAGCCTCATGAATACACTC
CCTCATGGAAAGCATGGAGTCTGTCCAGCCTTCCTATGATTCCACCTCGTTTCGGCATTAAGCTAATCAGCGATCCGGGC
ATTGAAAAACAATTTCGTATTATAGAAGGACTGATGCAAAATGCCGATGAAATTATAAATTGCGGTGATGCCGGCCAGGA
AGGAGAACTTATTCAACGATGGGTCATGCAGAAAGCAGGAGCACACTGCCCGGTGAAACGTCTATGGATTTCATCCTTGA
CTGAAGAAGCCATACGCGAAGGATTCAGCAAACTAAAAGATCAAAAAGAATTCCAACCTTTATACGAAGCTGGATTAAGC
CGTGCTATCGGCGATTGGGTATTGGGCATGAACGCCACCCGGCTCTATACCTTAAAGTACGGACAAAACAGACAAATTCT
ATCGATAGGACGGGTGCAGACTCCGACTTTGGCACTGATAGTAAAGCGTCAGCAAGAAATAGAGAACTTCACTCCTCAAC
AATATTGGGTACTGGCTACTTTATACCGGGAAACCACATTCTCTGCCATCATCCGCAAAAGCGATGAGGAACTGGCTCAG
GAAGAAAGCGATGCTAAAGAGAACCCCAAGAAAGCCAAAAAGGCTGAAGGGAACAGGGGAATTCCTCCCATCACCGATCT
TGCTACCGGACAGGCACTTATGGAACGTATCAAAAATGTTCCTTTTACCGTGACAGAAGTAGCAAAAAAACAAGGCACAG
AAGCACCTCCCCGTCTTTTTGACCTGACTTCCCTACAGGTGGAATGTAACAAAAAATTTGCTTATTCAGCTGACGAAACT
CTAAAACTTATCCAGTCATTGTATGAAAAGAAAGTGACTACTTATCCTCGTGTAGATACTACTTTCTTAAGCGATGACAT
CTATCCCAAATGTCCCAATATCTTGGCGGGACTAACTCCATATACAGTATTTACCGCTCCGTTGGCAGGACAAAAGCTCA
TCAAGTCCAAAAAAGTATTTGACAATTCAAAAGTAACGGATCACCATGCCATTATTCCTACGGGACAACCTCCTGTAAAT
CTGACAGAAATGGAGAAACGGGTATTTGATTTGGTAGCACGCCGCTTTATTGCCGTATTCTATCCCGACTGCAAGTTTGC
CACTACTACCGTAATAGGCGAAGCTGACAGTATAGAATTCAAAGCCACAGGAAAACAGATTCTAGATCCCGGATGGCGCG
TTATCTTTGCCAAACCACAAACCAATCTGCCAGAAGGTATGACCGATCCCGATGCAGAAGCCCGTGAAAATGACGAAGAA
CACTCCTTGCCCGCTTTTGTGAAAGGAGAAAGCGGGCCGCATCTGCCCAAACTGGACGAGAAGTGGACACAACCCCCACG
GCCATATACAGAAGCAACTTTACTACGCGCTATGGAAACTGCCGGTAAGCTGGTAGATAATGACGAGCTACGCGATGCAT
TAAAAGAAAATGGAATAGGACGTCCGTCTACACGTGCAGCCATTATTGAAACCCTATTTAAACGCCATTATATCCGTAAA
GAACGTAAAAATCTGATCGCAACCCCTACCGGAATAGAACTGGTGGGACTAATTCGCGAAGAACTGTTGAAATCCGCCGA
ATTAACGGGAATATGGGAAAAGAAACTACGTGAAATAGAAAAGAAAAGCTATGATGCAACGACTTTTCTAAATGAATTAA
AGCAAATGGTAAGTGAAATAGTATACAGCGTTTTAAGTGATAATACAAACCGCCGCGTCACCACAATAGAGGAAACAGCA
ACGAAAACTGCAACAACCTCTACGGTCAAACAGCCGAAAAAAAAGGCAGGGACCTCACGAAAAAACGCTTCTTCAGCCAC
GCCCGCTCCCACTATCCCATCCGATAACGAATTGGTGGGAAAATCCTGCCCGCTATGTGGCAAAGGAACGATTATAAAAG
GAAAAACCGCGTATGGCTGCTCAGAATGGAAAAACGGGTGTACCTACAGGAAGCCTTTCTGA

Upstream 100 bases:

>100_bases
TTAAATTCTTATAAAAGAAGAACTAATAAAGACAAAACAAAATCTCTTCCGAGGTTTTTATTACCTTTGCAATCCTATTT
GAACAGAAACGAAAAAAAAT

Downstream 100 bases:

>100_bases
TAATCACATTTTCCTTGTACGAAGCCAAACAAATTTGTATATTATAGAATACAAACAAGTCTGTACCCGGAGAAGCATTG
CGCCAGTCTATGAAAAACAA

Product: putative DNA topoisomerase

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MIVCIAEKPSVARDIADVLGAKTKKDGYIEGNGYQVTWTFGHLCTLKEPHEYTPSWKAWSLSSLPMIPPRFGIKLISDPG
IEKQFRIIEGLMQNADEIINCGDAGQEGELIQRWVMQKAGAHCPVKRLWISSLTEEAIREGFSKLKDQKEFQPLYEAGLS
RAIGDWVLGMNATRLYTLKYGQNRQILSIGRVQTPTLALIVKRQQEIENFTPQQYWVLATLYRETTFSAIIRKSDEELAQ
EESDAKENPKKAKKAEGNRGIPPITDLATGQALMERIKNVPFTVTEVAKKQGTEAPPRLFDLTSLQVECNKKFAYSADET
LKLIQSLYEKKVTTYPRVDTTFLSDDIYPKCPNILAGLTPYTVFTAPLAGQKLIKSKKVFDNSKVTDHHAIIPTGQPPVN
LTEMEKRVFDLVARRFIAVFYPDCKFATTTVIGEADSIEFKATGKQILDPGWRVIFAKPQTNLPEGMTDPDAEARENDEE
HSLPAFVKGESGPHLPKLDEKWTQPPRPYTEATLLRAMETAGKLVDNDELRDALKENGIGRPSTRAAIIETLFKRHYIRK
ERKNLIATPTGIELVGLIREELLKSAELTGIWEKKLREIEKKSYDATTFLNELKQMVSEIVYSVLSDNTNRRVTTIEETA
TKTATTSTVKQPKKKAGTSRKNASSATPAPTIPSDNELVGKSCPLCGKGTIIKGKTAYGCSEWKNGCTYRKPF

Sequences:

>Translated_713_residues
MIVCIAEKPSVARDIADVLGAKTKKDGYIEGNGYQVTWTFGHLCTLKEPHEYTPSWKAWSLSSLPMIPPRFGIKLISDPG
IEKQFRIIEGLMQNADEIINCGDAGQEGELIQRWVMQKAGAHCPVKRLWISSLTEEAIREGFSKLKDQKEFQPLYEAGLS
RAIGDWVLGMNATRLYTLKYGQNRQILSIGRVQTPTLALIVKRQQEIENFTPQQYWVLATLYRETTFSAIIRKSDEELAQ
EESDAKENPKKAKKAEGNRGIPPITDLATGQALMERIKNVPFTVTEVAKKQGTEAPPRLFDLTSLQVECNKKFAYSADET
LKLIQSLYEKKVTTYPRVDTTFLSDDIYPKCPNILAGLTPYTVFTAPLAGQKLIKSKKVFDNSKVTDHHAIIPTGQPPVN
LTEMEKRVFDLVARRFIAVFYPDCKFATTTVIGEADSIEFKATGKQILDPGWRVIFAKPQTNLPEGMTDPDAEARENDEE
HSLPAFVKGESGPHLPKLDEKWTQPPRPYTEATLLRAMETAGKLVDNDELRDALKENGIGRPSTRAAIIETLFKRHYIRK
ERKNLIATPTGIELVGLIREELLKSAELTGIWEKKLREIEKKSYDATTFLNELKQMVSEIVYSVLSDNTNRRVTTIEETA
TKTATTSTVKQPKKKAGTSRKNASSATPAPTIPSDNELVGKSCPLCGKGTIIKGKTAYGCSEWKNGCTYRKPF
>Mature_713_residues
MIVCIAEKPSVARDIADVLGAKTKKDGYIEGNGYQVTWTFGHLCTLKEPHEYTPSWKAWSLSSLPMIPPRFGIKLISDPG
IEKQFRIIEGLMQNADEIINCGDAGQEGELIQRWVMQKAGAHCPVKRLWISSLTEEAIREGFSKLKDQKEFQPLYEAGLS
RAIGDWVLGMNATRLYTLKYGQNRQILSIGRVQTPTLALIVKRQQEIENFTPQQYWVLATLYRETTFSAIIRKSDEELAQ
EESDAKENPKKAKKAEGNRGIPPITDLATGQALMERIKNVPFTVTEVAKKQGTEAPPRLFDLTSLQVECNKKFAYSADET
LKLIQSLYEKKVTTYPRVDTTFLSDDIYPKCPNILAGLTPYTVFTAPLAGQKLIKSKKVFDNSKVTDHHAIIPTGQPPVN
LTEMEKRVFDLVARRFIAVFYPDCKFATTTVIGEADSIEFKATGKQILDPGWRVIFAKPQTNLPEGMTDPDAEARENDEE
HSLPAFVKGESGPHLPKLDEKWTQPPRPYTEATLLRAMETAGKLVDNDELRDALKENGIGRPSTRAAIIETLFKRHYIRK
ERKNLIATPTGIELVGLIREELLKSAELTGIWEKKLREIEKKSYDATTFLNELKQMVSEIVYSVLSDNTNRRVTTIEETA
TKTATTSTVKQPKKKAGTSRKNASSATPAPTIPSDNELVGKSCPLCGKGTIIKGKTAYGCSEWKNGCTYRKPF

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=624, Percent_Identity=25.3205128205128, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1788061, Length=699, Percent_Identity=31.7596566523605, Blast_Score=312, Evalue=5e-86,
Organism=Escherichia coli, GI1787529, Length=535, Percent_Identity=25.7943925233645, Blast_Score=130, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32563869, Length=611, Percent_Identity=23.5679214402619, Blast_Score=112, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79855; Mature: 79855

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVCIAEKPSVARDIADVLGAKTKKDGYIEGNGYQVTWTFGHLCTLKEPHEYTPSWKAWS
CEEEEECCCHHHHHHHHHHCCCCCCCCEEECCCEEEEEEECCEEECCCCCCCCCCCCCCC
LSSLPMIPPRFGIKLISDPGIEKQFRIIEGLMQNADEIINCGDAGQEGELIQRWVMQKAG
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHCC
AHCPVKRLWISSLTEEAIREGFSKLKDQKEFQPLYEAGLSRAIGDWVLGMNATRLYTLKY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
GQNRQILSIGRVQTPTLALIVKRQQEIENFTPQQYWVLATLYRETTFSAIIRKSDEELAQ
CCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
EESDAKENPKKAKKAEGNRGIPPITDLATGQALMERIKNVPFTVTEVAKKQGTEAPPRLF
HHHHHHCCCHHHHHCCCCCCCCCCHHHHCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEE
DLTSLQVECNKKFAYSADETLKLIQSLYEKKVTTYPRVDTTFLSDDIYPKCPNILAGLTP
EEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCHHHHCCCC
YTVFTAPLAGQKLIKSKKVFDNSKVTDHHAIIPTGQPPVNLTEMEKRVFDLVARRFIAVF
HHEEECCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHC
YPDCKFATTTVIGEADSIEFKATGKQILDPGWRVIFAKPQTNLPEGMTDPDAEARENDEE
CCCCCCCCEEEECCCCCEEEEECCHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHCCCCH
HSLPAFVKGESGPHLPKLDEKWTQPPRPYTEATLLRAMETAGKLVDNDELRDALKENGIG
HCCCEEEECCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
RPSTRAAIIETLFKRHYIRKERKNLIATPTGIELVGLIREELLKSAELTGIWEKKLREIE
CCHHHHHHHHHHHHHHHHHHHHHCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKSYDATTFLNELKQMVSEIVYSVLSDNTNRRVTTIEETATKTATTSTVKQPKKKAGTSR
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
KNASSATPAPTIPSDNELVGKSCPLCGKGTIIKGKTAYGCSEWKNGCTYRKPF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MIVCIAEKPSVARDIADVLGAKTKKDGYIEGNGYQVTWTFGHLCTLKEPHEYTPSWKAWS
CEEEEECCCHHHHHHHHHHCCCCCCCCEEECCCEEEEEEECCEEECCCCCCCCCCCCCCC
LSSLPMIPPRFGIKLISDPGIEKQFRIIEGLMQNADEIINCGDAGQEGELIQRWVMQKAG
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHCC
AHCPVKRLWISSLTEEAIREGFSKLKDQKEFQPLYEAGLSRAIGDWVLGMNATRLYTLKY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
GQNRQILSIGRVQTPTLALIVKRQQEIENFTPQQYWVLATLYRETTFSAIIRKSDEELAQ
CCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
EESDAKENPKKAKKAEGNRGIPPITDLATGQALMERIKNVPFTVTEVAKKQGTEAPPRLF
HHHHHHCCCHHHHHCCCCCCCCCCHHHHCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEE
DLTSLQVECNKKFAYSADETLKLIQSLYEKKVTTYPRVDTTFLSDDIYPKCPNILAGLTP
EEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCHHHHCCCC
YTVFTAPLAGQKLIKSKKVFDNSKVTDHHAIIPTGQPPVNLTEMEKRVFDLVARRFIAVF
HHEEECCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHC
YPDCKFATTTVIGEADSIEFKATGKQILDPGWRVIFAKPQTNLPEGMTDPDAEARENDEE
CCCCCCCCEEEECCCCCEEEEECCHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHCCCCH
HSLPAFVKGESGPHLPKLDEKWTQPPRPYTEATLLRAMETAGKLVDNDELRDALKENGIG
HCCCEEEECCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
RPSTRAAIIETLFKRHYIRKERKNLIATPTGIELVGLIREELLKSAELTGIWEKKLREIE
CCHHHHHHHHHHHHHHHHHHHHHCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKSYDATTFLNELKQMVSEIVYSVLSDNTNRRVTTIEETATKTATTSTVKQPKKKAGTSR
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
KNASSATPAPTIPSDNELVGKSCPLCGKGTIIKGKTAYGCSEWKNGCTYRKPF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA