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The map label for this gene is ndhF [H]

Identifier: 150004306

GI number: 150004306

Start: 2249647

End: 2251548

Strand: Reverse

Name: ndhF [H]

Synonym: BVU_1752

Alternate gene names: 150004306

Gene position: 2251548-2249647 (Counterclockwise)

Preceding gene: 150004307

Following gene: 150004305

Centisome position: 43.61

GC content: 44.95

Gene sequence:

>1902_bases
ATGGAACATACAATACTAATCCTTATCCTACCTTTCCTCAGTTTCCTGATACTGGGGCTGGCAGGTATGCGTATGAAGAA
CGGATTGGCAGGAACCATCGGCACCGTTTCACTGGCAGGGGTGACGTTGCTTTCCTACCTCACTGCCTTCAATTATTTTG
CAGGAGGGCGTACGGCAGAAGGCGTCTATCCTACTCTCACACCTTATAATTTTGAATGGTTACCTTTCACAACGAACCTG
CATATTGACATGGGAATCATGCTTGACCCTATTTCAGTTATGATGCTAGTCGTTATTTCCACCGTCAGTCTGATGGTACA
TATCTATTCATTCGGATATATGAAAGGTGAAAAAGGCTTTCAACGTTACTACGCATTTCTTAGCCTGTTTACCATGTCCA
TGTTGGGACTCGTAGTAGCAACTAATATTTTCCAGATGTATGTCTTTTGGGAACTGGTAGGTGTTTCTTCTTATCTACTT
ATCGGCTTTTATTACACCAAGAAAGAAGCGATTGCTGCCAGTAAAAAAGCATTTATTGTTACCCGTTTCGCTGATTTAGG
CTTTCTGATCGGTATCTTGATTTACGGCTATTATGCCGAAACATTCTCGTTCACCCCCCAACTTCAGGTACTTGCAGTTG
CCGGAAGTATGATACCATTGGCCTTGGGGCTAATGTTTATCGGTGGTGCCGGTAAAAGTGCGATGTTTCCATTGCACATC
TGGTTACCGGATGCTATGGAAGGTCCTACTCCTGTCAGTGCATTAATCCATGCTGCTACCATGGTAGTAGCCGGTGTATA
TCTGGTAGCCCGTATGTTCCCGTTGTTTATAGGATATGCGCCCGAAGTGCTGCACTGGATAGCTTATATCGGTGCGTTCA
CTGCATTCTACGCCGCTAGTGTGGCGTGTGCACAAAGCGATATCAAACGTGTGCTCGCCTTCTCCACTATATCTCAAATT
GGATTTATGATTGTTGCATTAGGCGTTTGTACTTCCATGAATCCTCATGAAGGAGGACTAGGTTACATGGCATCCATGTT
CCACCTGTTCACACATGCCATGTTCAAGGCATTGCTGTTCTTAGGAGCCGGATGTATCATTCATGCCGTACACAGTAACG
AGATGAGCGCCATGGGAGGACTTCATAAATTCATGCCCGTCACCCACTGGACATTCCTGATTGCTTGTCTGGCTATCTCG
GGTATTTGGCCATTCTCAGGTTTTTTCTCTAAAGACGAAATTCTGACTGCCTGCTTCCAATTCAGTCCTGTTATGGGATG
GATAATGACAGGCATTGCCGCCATGACAGCATTTTATATGTTCCGCCTTTATTATTGTATTTTCTGGAATGGAGAATGGA
AAGGACACTCCCACGAATCCGGACCTGATGCACATACTCCGCATGAGGCTCCACGTACCATGACATTCCCGCTGATGTTT
TTAGCCGCTATCACCTGTATTGCCGGATTCATACCCTTCGGCAATCTGATCAGTAGTAACGGAGAAGCCTATACCATTCA
TCTGGATATACAGATAGCTGCTACAAGTATCATTATTGCTATAGCATCCATCACTTTAGCTACATGGATGTATGCAGGTA
AGAGACAACCCGTAGCCAACTATCTGGCGCGCACATTCCCACGTTTGCACACAGCAGCTTACCACCGTTTCTATATGGAT
GAGATATGGTTGTTCGTAACCAAGAAAATCATCTTCCGGTGCATCAGTACTCCGATAGCTTGGTGGGACCGGCACGTTAT
CGATCAATTCTTTAACTTCACTGCGTGGAGTACTCATGCTACCGCCGATGAAATACGTGACATGCAAAGTGGAAACGTAC
AGCAGTACAGTATTTGGTTCCTTGCAGGTGCATTGATATTGACGCTGATACTATTAATTTAA

Upstream 100 bases:

>100_bases
GTGCGGCTGAAACAGCAGTAGCTATTGCTATCATTATCAATATCTACCGCAATGTACGCAATATCCAAGTGAAGAACTTA
AATGAAATGAAAAACTAATT

Downstream 100 bases:

>100_bases
TGAATAATAAAAAATCCGCCGCATGGCCATTTTTTAATTTTTAATTTAAAAAGATGAACATACTAAGTTTTTTCATTATA
GTTCCCTTGTTGATGCTCGC

Product: putative NADH dehydrogenase chain L

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MEHTILILILPFLSFLILGLAGMRMKNGLAGTIGTVSLAGVTLLSYLTAFNYFAGGRTAEGVYPTLTPYNFEWLPFTTNL
HIDMGIMLDPISVMMLVVISTVSLMVHIYSFGYMKGEKGFQRYYAFLSLFTMSMLGLVVATNIFQMYVFWELVGVSSYLL
IGFYYTKKEAIAASKKAFIVTRFADLGFLIGILIYGYYAETFSFTPQLQVLAVAGSMIPLALGLMFIGGAGKSAMFPLHI
WLPDAMEGPTPVSALIHAATMVVAGVYLVARMFPLFIGYAPEVLHWIAYIGAFTAFYAASVACAQSDIKRVLAFSTISQI
GFMIVALGVCTSMNPHEGGLGYMASMFHLFTHAMFKALLFLGAGCIIHAVHSNEMSAMGGLHKFMPVTHWTFLIACLAIS
GIWPFSGFFSKDEILTACFQFSPVMGWIMTGIAAMTAFYMFRLYYCIFWNGEWKGHSHESGPDAHTPHEAPRTMTFPLMF
LAAITCIAGFIPFGNLISSNGEAYTIHLDIQIAATSIIIAIASITLATWMYAGKRQPVANYLARTFPRLHTAAYHRFYMD
EIWLFVTKKIIFRCISTPIAWWDRHVIDQFFNFTAWSTHATADEIRDMQSGNVQQYSIWFLAGALILTLILLI

Sequences:

>Translated_633_residues
MEHTILILILPFLSFLILGLAGMRMKNGLAGTIGTVSLAGVTLLSYLTAFNYFAGGRTAEGVYPTLTPYNFEWLPFTTNL
HIDMGIMLDPISVMMLVVISTVSLMVHIYSFGYMKGEKGFQRYYAFLSLFTMSMLGLVVATNIFQMYVFWELVGVSSYLL
IGFYYTKKEAIAASKKAFIVTRFADLGFLIGILIYGYYAETFSFTPQLQVLAVAGSMIPLALGLMFIGGAGKSAMFPLHI
WLPDAMEGPTPVSALIHAATMVVAGVYLVARMFPLFIGYAPEVLHWIAYIGAFTAFYAASVACAQSDIKRVLAFSTISQI
GFMIVALGVCTSMNPHEGGLGYMASMFHLFTHAMFKALLFLGAGCIIHAVHSNEMSAMGGLHKFMPVTHWTFLIACLAIS
GIWPFSGFFSKDEILTACFQFSPVMGWIMTGIAAMTAFYMFRLYYCIFWNGEWKGHSHESGPDAHTPHEAPRTMTFPLMF
LAAITCIAGFIPFGNLISSNGEAYTIHLDIQIAATSIIIAIASITLATWMYAGKRQPVANYLARTFPRLHTAAYHRFYMD
EIWLFVTKKIIFRCISTPIAWWDRHVIDQFFNFTAWSTHATADEIRDMQSGNVQQYSIWFLAGALILTLILLI
>Mature_633_residues
MEHTILILILPFLSFLILGLAGMRMKNGLAGTIGTVSLAGVTLLSYLTAFNYFAGGRTAEGVYPTLTPYNFEWLPFTTNL
HIDMGIMLDPISVMMLVVISTVSLMVHIYSFGYMKGEKGFQRYYAFLSLFTMSMLGLVVATNIFQMYVFWELVGVSSYLL
IGFYYTKKEAIAASKKAFIVTRFADLGFLIGILIYGYYAETFSFTPQLQVLAVAGSMIPLALGLMFIGGAGKSAMFPLHI
WLPDAMEGPTPVSALIHAATMVVAGVYLVARMFPLFIGYAPEVLHWIAYIGAFTAFYAASVACAQSDIKRVLAFSTISQI
GFMIVALGVCTSMNPHEGGLGYMASMFHLFTHAMFKALLFLGAGCIIHAVHSNEMSAMGGLHKFMPVTHWTFLIACLAIS
GIWPFSGFFSKDEILTACFQFSPVMGWIMTGIAAMTAFYMFRLYYCIFWNGEWKGHSHESGPDAHTPHEAPRTMTFPLMF
LAAITCIAGFIPFGNLISSNGEAYTIHLDIQIAATSIIIAIASITLATWMYAGKRQPVANYLARTFPRLHTAAYHRFYMD
EIWLFVTKKIIFRCISTPIAWWDRHVIDQFFNFTAWSTHATADEIRDMQSGNVQQYSIWFLAGALILTLILLI

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=451, Percent_Identity=34.1463414634146, Blast_Score=222, Evalue=8e-58,
Organism=Escherichia coli, GI1788614, Length=561, Percent_Identity=37.9679144385027, Blast_Score=311, Evalue=9e-86,
Organism=Escherichia coli, GI1788829, Length=503, Percent_Identity=31.2127236580517, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788827, Length=426, Percent_Identity=30.5164319248826, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788831, Length=351, Percent_Identity=30.1994301994302, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788613, Length=377, Percent_Identity=29.4429708222812, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI2367154, Length=430, Percent_Identity=23.4883720930233, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI145693160, Length=236, Percent_Identity=27.9661016949153, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 70353; Mature: 70353

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHTILILILPFLSFLILGLAGMRMKNGLAGTIGTVSLAGVTLLSYLTAFNYFAGGRTAE
CCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GVYPTLTPYNFEWLPFTTNLHIDMGIMLDPISVMMLVVISTVSLMVHIYSFGYMKGEKGF
CCCCCCCCCCEEEEEEEEEEEEEECCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
QRYYAFLSLFTMSMLGLVVATNIFQMYVFWELVGVSSYLLIGFYYTKKEAIAASKKAFIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHCCCCEEE
TRFADLGFLIGILIYGYYAETFSFTPQLQVLAVAGSMIPLALGLMFIGGAGKSAMFPLHI
EHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
WLPDAMEGPTPVSALIHAATMVVAGVYLVARMFPLFIGYAPEVLHWIAYIGAFTAFYAAS
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VACAQSDIKRVLAFSTISQIGFMIVALGVCTSMNPHEGGLGYMASMFHLFTHAMFKALLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGAGCIIHAVHSNEMSAMGGLHKFMPVTHWTFLIACLAISGIWPFSGFFSKDEILTACFQ
HCCCHHEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
FSPVMGWIMTGIAAMTAFYMFRLYYCIFWNGEWKGHSHESGPDAHTPHEAPRTMTFPLMF
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LAAITCIAGFIPFGNLISSNGEAYTIHLDIQIAATSIIIAIASITLATWMYAGKRQPVAN
HHHHHHHHHHHCCCHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHH
YLARTFPRLHTAAYHRFYMDEIWLFVTKKIIFRCISTPIAWWDRHVIDQFFNFTAWSTHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCC
TADEIRDMQSGNVQQYSIWFLAGALILTLILLI
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MEHTILILILPFLSFLILGLAGMRMKNGLAGTIGTVSLAGVTLLSYLTAFNYFAGGRTAE
CCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GVYPTLTPYNFEWLPFTTNLHIDMGIMLDPISVMMLVVISTVSLMVHIYSFGYMKGEKGF
CCCCCCCCCCEEEEEEEEEEEEEECCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
QRYYAFLSLFTMSMLGLVVATNIFQMYVFWELVGVSSYLLIGFYYTKKEAIAASKKAFIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHCCCCEEE
TRFADLGFLIGILIYGYYAETFSFTPQLQVLAVAGSMIPLALGLMFIGGAGKSAMFPLHI
EHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
WLPDAMEGPTPVSALIHAATMVVAGVYLVARMFPLFIGYAPEVLHWIAYIGAFTAFYAAS
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VACAQSDIKRVLAFSTISQIGFMIVALGVCTSMNPHEGGLGYMASMFHLFTHAMFKALLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGAGCIIHAVHSNEMSAMGGLHKFMPVTHWTFLIACLAISGIWPFSGFFSKDEILTACFQ
HCCCHHEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
FSPVMGWIMTGIAAMTAFYMFRLYYCIFWNGEWKGHSHESGPDAHTPHEAPRTMTFPLMF
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LAAITCIAGFIPFGNLISSNGEAYTIHLDIQIAATSIIIAIASITLATWMYAGKRQPVAN
HHHHHHHHHHHCCCHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHH
YLARTFPRLHTAAYHRFYMDEIWLFVTKKIIFRCISTPIAWWDRHVIDQFFNFTAWSTHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCC
TADEIRDMQSGNVQQYSIWFLAGALILTLILLI
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]