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The map label for this gene is topB [H]

Identifier: 150004088

GI number: 150004088

Start: 2009590

End: 2011362

Strand: Reverse

Name: topB [H]

Synonym: BVU_1523

Alternate gene names: 150004088

Gene position: 2011362-2009590 (Counterclockwise)

Preceding gene: 150004089

Following gene: 150004087

Centisome position: 38.96

GC content: 57.13

Gene sequence:

>1773_bases
ATGATCGCAATAGTGACTGACAGGCCCAACGTGGGCAGAGAGATAGCAAGAGTGTTGGGAGCAGGCAGAAAGGAGAACGG
CTATATGACCGGGAACGGCTACATGGTGACATGGACATACGGCAACATGCTCTCCCTGGCAATGCCGAAGGATTCAGGAA
CAGCCCGTGTGGAGCGGGAGGACCTTCCCCTGCTGCCGCCTTCCGTCCTGACAGTAAGGCACGTGAAGACGGATACCGGG
TGGAATCCCGACATCAATGCGGTGCTCCAGTTGAAAGTGATAGCCAAGGTACTCAACGCATGCGACACCATCATCGCGGC
AACCGACGCTTCCCGGGAGGGAGAGATGCTGTTCCGCCATCTTTACGGCTTTCTGGGGTGCAAACAACCCTGCCTCCGCC
TCTGGATCTCGTCTCTGACGGACGAGGCCATCACGGAAGGCATGGCGAACCTCCTTCCATGTGACCGGTTCGACAACCTG
TTCCTGGCGGCGGACAGCCGTAACCGGGCGGACTGGCTCCTGGGCGTCAATTCAAGCCATGCCATCTGCAAGGCGGTAGG
CTTCGGGAACAACTCGCTGGGCAGGGTACAGACCCCCGTACTGGCGGAGATATGCAGACGGTACCGGGAGAGGGAGAACC
ACATGCCGGCTGACAGCTGGCCCGTATTCATCAGCCTATGCAAGAATGACCGTATCCTAAAGATGCGCCACGTGGACGAC
CTTGTGGCCCGCCGGGAGGCACTGGCATTGTACGAGGATTGCAAGGCGGCAAAGAACGCCCGCATCACAGCGGTAAGGAA
ACGGACGGAGGAAATCGGGGCACCGGCACTTTACAACCTGGCCGAACTGCAGAAAGACGCGAACCGTTACCACGGCCTGA
CGGCCATACAGGTGCAGGAGATTGCACAGAGCCTGTACGAGAAGAGGCTGATTTCCTATCCGCGCACCTCCAGTCGCCTG
TTGCCGGGGGATGTATATGACACGTTGCCGCCTGTCATGGAGAAGATGCTCTCCCGCAAGGAGTTCCGACAATATGCCGG
TATGGTTGACCTTGCCGCGCCGCAGGACAGCATTGGGGCACAAGACACCATGGAGCATCATGCCATCATCATTACAGGTA
TACAGCCCGGTAAATTGGGCCGGGAGGAAATGCAGGTTTACACGCTCATTGTCGGCAGGGTGCTTGAAACGTTCATGCCG
CCCTGCAAGGTGGAATACACAACCGTCGATGCGGTGTGCGCCGCACGTAAATTCCGCATCCGTACATACCGCATCCTGGA
AAAAGGATGGCTCGGAATCTTCGAGAGGGAACATATTGTCGCAAAAGGCTGTATGCCCTGCCCGATGCTGCCGGAATTTT
TTCAGGAAGAGATACTGCCGGTGGCAGGGTGCAGCCTTATACATAAGAAATCGTTACCGGTCTCCCCTTATACAGACGAG
GAACTGGTGGACTACATGGACAAGGCCGGACTGGGCACCGTCTCCACCCGCACGAATATCCTCCGCACGCTGCTGGAACG
TAAATACATCCGTTATTCGGGTAAATATGTCGTCCCGACCCCTAAAGGGCTTTTCCTGTATGAGACGGTACGCGGTATGA
AGGTGGCCGACGCCTCCCTCACCTCGGGCTGGGAAACTGAACTGGCACGGATAGAACGGGGGGAACTCTCGCAGGAAGAG
TTCCTGGACGGCGTGCTGGAAACGGTAAATGAGGTGACAGGCGAGATATTCCGGAACCATCCGGTGGAAAAGCGGCCACA
GGGAACCATATAA

Upstream 100 bases:

>100_bases
CCGTCAGGAGGAATCTCCAAAGAAATAACAAAAAATAAAACAAGTTTAACATGTCCCCGTATCGCCCAATCCGGTACGGG
GCATCAAAAAGAAAAAGAAT

Downstream 100 bases:

>100_bases
ACAAATGTCAAAGCCAACAAGACAAGGGAAGTGCAGACGTCTGTCTGTACTTCCTTTTTATTTTTTATATGTGCAAGATA
CGTTTTTCCGATTTTAAGCG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MIAIVTDRPNVGREIARVLGAGRKENGYMTGNGYMVTWTYGNMLSLAMPKDSGTARVEREDLPLLPPSVLTVRHVKTDTG
WNPDINAVLQLKVIAKVLNACDTIIAATDASREGEMLFRHLYGFLGCKQPCLRLWISSLTDEAITEGMANLLPCDRFDNL
FLAADSRNRADWLLGVNSSHAICKAVGFGNNSLGRVQTPVLAEICRRYRERENHMPADSWPVFISLCKNDRILKMRHVDD
LVARREALALYEDCKAAKNARITAVRKRTEEIGAPALYNLAELQKDANRYHGLTAIQVQEIAQSLYEKRLISYPRTSSRL
LPGDVYDTLPPVMEKMLSRKEFRQYAGMVDLAAPQDSIGAQDTMEHHAIIITGIQPGKLGREEMQVYTLIVGRVLETFMP
PCKVEYTTVDAVCAARKFRIRTYRILEKGWLGIFEREHIVAKGCMPCPMLPEFFQEEILPVAGCSLIHKKSLPVSPYTDE
ELVDYMDKAGLGTVSTRTNILRTLLERKYIRYSGKYVVPTPKGLFLYETVRGMKVADASLTSGWETELARIERGELSQEE
FLDGVLETVNEVTGEIFRNHPVEKRPQGTI

Sequences:

>Translated_590_residues
MIAIVTDRPNVGREIARVLGAGRKENGYMTGNGYMVTWTYGNMLSLAMPKDSGTARVEREDLPLLPPSVLTVRHVKTDTG
WNPDINAVLQLKVIAKVLNACDTIIAATDASREGEMLFRHLYGFLGCKQPCLRLWISSLTDEAITEGMANLLPCDRFDNL
FLAADSRNRADWLLGVNSSHAICKAVGFGNNSLGRVQTPVLAEICRRYRERENHMPADSWPVFISLCKNDRILKMRHVDD
LVARREALALYEDCKAAKNARITAVRKRTEEIGAPALYNLAELQKDANRYHGLTAIQVQEIAQSLYEKRLISYPRTSSRL
LPGDVYDTLPPVMEKMLSRKEFRQYAGMVDLAAPQDSIGAQDTMEHHAIIITGIQPGKLGREEMQVYTLIVGRVLETFMP
PCKVEYTTVDAVCAARKFRIRTYRILEKGWLGIFEREHIVAKGCMPCPMLPEFFQEEILPVAGCSLIHKKSLPVSPYTDE
ELVDYMDKAGLGTVSTRTNILRTLLERKYIRYSGKYVVPTPKGLFLYETVRGMKVADASLTSGWETELARIERGELSQEE
FLDGVLETVNEVTGEIFRNHPVEKRPQGTI
>Mature_590_residues
MIAIVTDRPNVGREIARVLGAGRKENGYMTGNGYMVTWTYGNMLSLAMPKDSGTARVEREDLPLLPPSVLTVRHVKTDTG
WNPDINAVLQLKVIAKVLNACDTIIAATDASREGEMLFRHLYGFLGCKQPCLRLWISSLTDEAITEGMANLLPCDRFDNL
FLAADSRNRADWLLGVNSSHAICKAVGFGNNSLGRVQTPVLAEICRRYRERENHMPADSWPVFISLCKNDRILKMRHVDD
LVARREALALYEDCKAAKNARITAVRKRTEEIGAPALYNLAELQKDANRYHGLTAIQVQEIAQSLYEKRLISYPRTSSRL
LPGDVYDTLPPVMEKMLSRKEFRQYAGMVDLAAPQDSIGAQDTMEHHAIIITGIQPGKLGREEMQVYTLIVGRVLETFMP
PCKVEYTTVDAVCAARKFRIRTYRILEKGWLGIFEREHIVAKGCMPCPMLPEFFQEEILPVAGCSLIHKKSLPVSPYTDE
ELVDYMDKAGLGTVSTRTNILRTLLERKYIRYSGKYVVPTPKGLFLYETVRGMKVADASLTSGWETELARIERGELSQEE
FLDGVLETVNEVTGEIFRNHPVEKRPQGTI

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=482, Percent_Identity=22.8215767634855, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1788061, Length=633, Percent_Identity=27.6461295418641, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1787529, Length=439, Percent_Identity=24.6013667425968, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17555378, Length=523, Percent_Identity=24.282982791587, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI32563869, Length=591, Percent_Identity=23.6886632825719, Blast_Score=89, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323263, Length=410, Percent_Identity=23.4146341463415, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24640096, Length=574, Percent_Identity=22.6480836236934, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585251, Length=585, Percent_Identity=22.9059829059829, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 66391; Mature: 66391

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIVTDRPNVGREIARVLGAGRKENGYMTGNGYMVTWTYGNMLSLAMPKDSGTARVERE
CEEEEECCCCHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCEEEEEECCCCCCCEEECC
DLPLLPPSVLTVRHVKTDTGWNPDINAVLQLKVIAKVLNACDTIIAATDASREGEMLFRH
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHH
LYGFLGCKQPCLRLWISSLTDEAITEGMANLLPCDRFDNLFLAADSRNRADWLLGVNSSH
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCCCC
AICKAVGFGNNSLGRVQTPVLAEICRRYRERENHMPADSWPVFISLCKNDRILKMRHVDD
HHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHHH
LVARREALALYEDCKAAKNARITAVRKRTEEIGAPALYNLAELQKDANRYHGLTAIQVQE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHH
IAQSLYEKRLISYPRTSSRLLPGDVYDTLPPVMEKMLSRKEFRQYAGMVDLAAPQDSIGA
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCC
QDTMEHHAIIITGIQPGKLGREEMQVYTLIVGRVLETFMPPCKVEYTTVDAVCAARKFRI
HHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
RTYRILEKGWLGIFEREHIVAKGCMPCPMLPEFFQEEILPVAGCSLIHKKSLPVSPYTDE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHCHHHHHCCCCCCCCCCCH
ELVDYMDKAGLGTVSTRTNILRTLLERKYIRYSGKYVVPTPKGLFLYETVRGMKVADASL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEHHHHHCCCEEECCHH
TSGWETELARIERGELSQEEFLDGVLETVNEVTGEIFRNHPVEKRPQGTI
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIAIVTDRPNVGREIARVLGAGRKENGYMTGNGYMVTWTYGNMLSLAMPKDSGTARVERE
CEEEEECCCCHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCEEEEEECCCCCCCEEECC
DLPLLPPSVLTVRHVKTDTGWNPDINAVLQLKVIAKVLNACDTIIAATDASREGEMLFRH
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHH
LYGFLGCKQPCLRLWISSLTDEAITEGMANLLPCDRFDNLFLAADSRNRADWLLGVNSSH
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCCCC
AICKAVGFGNNSLGRVQTPVLAEICRRYRERENHMPADSWPVFISLCKNDRILKMRHVDD
HHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHHH
LVARREALALYEDCKAAKNARITAVRKRTEEIGAPALYNLAELQKDANRYHGLTAIQVQE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHH
IAQSLYEKRLISYPRTSSRLLPGDVYDTLPPVMEKMLSRKEFRQYAGMVDLAAPQDSIGA
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCC
QDTMEHHAIIITGIQPGKLGREEMQVYTLIVGRVLETFMPPCKVEYTTVDAVCAARKFRI
HHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
RTYRILEKGWLGIFEREHIVAKGCMPCPMLPEFFQEEILPVAGCSLIHKKSLPVSPYTDE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHCHHHHHCCCCCCCCCCCH
ELVDYMDKAGLGTVSTRTNILRTLLERKYIRYSGKYVVPTPKGLFLYETVRGMKVADASL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEHHHHHCCCEEECCHH
TSGWETELARIERGELSQEEFLDGVLETVNEVTGEIFRNHPVEKRPQGTI
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA