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The map label for this gene is pat [H]

Identifier: 150004026

GI number: 150004026

Start: 1940219

End: 1940704

Strand: Direct

Name: pat [H]

Synonym: BVU_1461

Alternate gene names: 150004026

Gene position: 1940219-1940704 (Clockwise)

Preceding gene: 150004022

Following gene: 150004027

Centisome position: 37.58

GC content: 45.47

Gene sequence:

>486_bases
ATGATTAGACGAGTGGAACTTCAGGATGCCAAAGCCATCACAACGATATACAATGAATATGTCGGGCACAGTGTGGCTAC
ATTTGAAACGGAACCTTTGAGAGAGGAGGATATGCGTAACCGTATTGCCGGAATTGCCGTCCGTTTTCCCTATTTTGTGT
ATGAGGAAGAAGGGAAAGTAGTGGGTTATTGTTATGCCCATTTGTGGAAGGAGAGGGCTGCTTACAGGCATACGTTGGAA
ACAACGGTCTATTTAGCTCCCGGATACGAGGGGAAGGGCATAGGCAGGGAGTTGATGGAAAGATTGATTGAAGAATGTCG
TAGGGACGGGTATCGTGCACTGATAGCCTGTGTCACAGAGGGAAATGTTGTCAGTGATGCTTTGCATTTGAGGCTGGGAT
TTAAAAAAGTTTCCCATTTTAAGAAGGTAGGACTGAAATTCGGACGATGGTTGGATGTAGTGGATTACGAGTTGCTGTTA
TCCTGA

Upstream 100 bases:

>100_bases
ATAGCTGAAATTAGAATCGGCTGTAAATATAGTGATTATTCTCCAGATAACTGATTATTTTTTTATATCTTGCTTACGTT
TTAATAATAGATATGACATT

Downstream 100 bases:

>100_bases
AACCACTGTCCGGAATGAACTTTTTAGGGTTATATGGAGTTATAAGCTTGTACCTAAATTAAAATAACCAACTATGACTC
AAGTAAACATCAAGAGGGTT

Product: putative sortase and related acyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MIRRVELQDAKAITTIYNEYVGHSVATFETEPLREEDMRNRIAGIAVRFPYFVYEEEGKVVGYCYAHLWKERAAYRHTLE
TTVYLAPGYEGKGIGRELMERLIEECRRDGYRALIACVTEGNVVSDALHLRLGFKKVSHFKKVGLKFGRWLDVVDYELLL
S

Sequences:

>Translated_161_residues
MIRRVELQDAKAITTIYNEYVGHSVATFETEPLREEDMRNRIAGIAVRFPYFVYEEEGKVVGYCYAHLWKERAAYRHTLE
TTVYLAPGYEGKGIGRELMERLIEECRRDGYRALIACVTEGNVVSDALHLRLGFKKVSHFKKVGLKFGRWLDVVDYELLL
S
>Mature_161_residues
MIRRVELQDAKAITTIYNEYVGHSVATFETEPLREEDMRNRIAGIAVRFPYFVYEEEGKVVGYCYAHLWKERAAYRHTLE
TTVYLAPGYEGKGIGRELMERLIEECRRDGYRALIACVTEGNVVSDALHLRLGFKKVSHFKKVGLKFGRWLDVVDYELLL
S

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=164, Percent_Identity=38.4146341463415, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 18685; Mature: 18685

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRVELQDAKAITTIYNEYVGHSVATFETEPLREEDMRNRIAGIAVRFPYFVYEEEGKV
CCEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHEEEECCEEEEECCCCE
VGYCYAHLWKERAAYRHTLETTVYLAPGYEGKGIGRELMERLIEECRRDGYRALIACVTE
EHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEEEEEECC
GNVVSDALHLRLGFKKVSHFKKVGLKFGRWLDVVDYELLLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MIRRVELQDAKAITTIYNEYVGHSVATFETEPLREEDMRNRIAGIAVRFPYFVYEEEGKV
CCEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHEEEECCEEEEECCCCE
VGYCYAHLWKERAAYRHTLETTVYLAPGYEGKGIGRELMERLIEECRRDGYRALIACVTE
EHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEEEEEECC
GNVVSDALHLRLGFKKVSHFKKVGLKFGRWLDVVDYELLLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA