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The map label for this gene is greA [H]

Identifier: 150003999

GI number: 150003999

Start: 1914137

End: 1914601

Strand: Direct

Name: greA [H]

Synonym: BVU_1433

Alternate gene names: 150003999

Gene position: 1914137-1914601 (Clockwise)

Preceding gene: 150003998

Following gene: 150004000

Centisome position: 37.07

GC content: 39.35

Gene sequence:

>465_bases
ATGGCTTATATGTCAGAAGAAGGTTACAAGCAACTGATTGAGGAACTGAAATATCTGGAATCAGTAGAACGTCCTAAAAT
TGTGTCGGCTATTGCCGAAGCGCGTGATAAGGGAGACTTGTCAGAAAATGCCGAATATGATGCTGCCAAAGAGGCGCAAG
GTCTATTGGAGATGAAGATCAGCCAGCTGAAATCAACTATTGGTGATGCTAAAATTATTGATACTTCAAAAATGAATGCA
GATACAGTGCAAATTTTGACGAAAGTAGAATTGAAGAATGCCAAGACCGGAATGAAGATGGTTTATACTATCGTAGCCGA
AAGCGAAGCGAATTTAAAATTGGGCAAGATTTCGGTAAACACTCCGATAGCACAGGGTTTGTTAGGAAAAAAAGTGGGTG
ATGTGGCTGAAATCACTATTCCGCAGGGTAAAATCAGCTTAGAAGTTGTGAATATTTCATTTTAA

Upstream 100 bases:

>100_bases
CCAAAGAGGGTTCCGACATTTCCATGTCGGAATTCTTTTTGTTTTTATAGGCATAACTGGAACAATGAATAATTATATAA
TTTAAAAGGAGGAAAACATT

Downstream 100 bases:

>100_bases
TATATAAGGCAGGTCCGCTATGCGGGGCTGCCTTATTTAATACTTATAGATATGGCAACAATATTTAGTAGAATTATTGC
AGGTGAGATTCCTAGCTATA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MAYMSEEGYKQLIEELKYLESVERPKIVSAIAEARDKGDLSENAEYDAAKEAQGLLEMKISQLKSTIGDAKIIDTSKMNA
DTVQILTKVELKNAKTGMKMVYTIVAESEANLKLGKISVNTPIAQGLLGKKVGDVAEITIPQGKISLEVVNISF

Sequences:

>Translated_154_residues
MAYMSEEGYKQLIEELKYLESVERPKIVSAIAEARDKGDLSENAEYDAAKEAQGLLEMKISQLKSTIGDAKIIDTSKMNA
DTVQILTKVELKNAKTGMKMVYTIVAESEANLKLGKISVNTPIAQGLLGKKVGDVAEITIPQGKISLEVVNISF
>Mature_153_residues
AYMSEEGYKQLIEELKYLESVERPKIVSAIAEARDKGDLSENAEYDAAKEAQGLLEMKISQLKSTIGDAKIIDTSKMNAD
TVQILTKVELKNAKTGMKMVYTIVAESEANLKLGKISVNTPIAQGLLGKKVGDVAEITIPQGKISLEVVNISF

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=43.421052631579, Blast_Score=127, Evalue=4e-31,
Organism=Escherichia coli, GI87082261, Length=151, Percent_Identity=32.4503311258278, Blast_Score=82, Evalue=1e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 16780; Mature: 16648

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYMSEEGYKQLIEELKYLESVERPKIVSAIAEARDKGDLSENAEYDAAKEAQGLLEMKI
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
SQLKSTIGDAKIIDTSKMNADTVQILTKVELKNAKTGMKMVYTIVAESEANLKLGKISVN
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHEEHHEEECCCCCEEEEEEECC
TPIAQGLLGKKVGDVAEITIPQGKISLEVVNISF
CCHHHHHHHHCCCCEEEEEECCCCEEEEEEEECC
>Mature Secondary Structure 
AYMSEEGYKQLIEELKYLESVERPKIVSAIAEARDKGDLSENAEYDAAKEAQGLLEMKI
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
SQLKSTIGDAKIIDTSKMNADTVQILTKVELKNAKTGMKMVYTIVAESEANLKLGKISVN
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHEEHHEEECCCCCEEEEEEECC
TPIAQGLLGKKVGDVAEITIPQGKISLEVVNISF
CCHHHHHHHHCCCCEEEEEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA