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The map label for this gene is rpoN [H]

Identifier: 150003985

GI number: 150003985

Start: 1894308

End: 1895777

Strand: Reverse

Name: rpoN [H]

Synonym: BVU_1419

Alternate gene names: 150003985

Gene position: 1895777-1894308 (Counterclockwise)

Preceding gene: 150003986

Following gene: 150003984

Centisome position: 36.72

GC content: 43.4

Gene sequence:

>1470_bases
ATGGCACAAGGCTCACGTCAAGTACAAACACAAGCCCAGCAACAAATACAAACCTTATCTCCACAACAGATACTGGTAGT
GAAATTGCTGGAACTGCCCACTGTGGAGCTGGAAGAACGTATCCATTCCGAAATACTGGATAATCCGGCCCTGGAGGAAG
GGAAAGAAATGCCCGAAAATGAAGATGACAACACAGAATACGTTGAGAACGAAGACGGAAATACAGACTCGAACGAAGAT
TTTTCATTAGGCGATTACAGCAACGAAGATGATATTCCCGATTATAAACTACAGGAACATAATCGTTCCAAAGAAGGAGT
AGCCGAAGAAATCCCTTTCTCGGATGCCGTATCTTTTTATGAAATATTGAAAGACCAGTTGCAGATGCAGGAACTTACCC
CAGAACAACGAGATATAGCAGAATACTTGATCGGTTCATTGGACGATGACGGCCTGTTACGCAAAAATATGGAGTCTATC
ATGGATGAACTTGCCATCTATCGAGGCATCTATACCACTGAGGAGGAATTAAATAAAATCTTGGAAATAATCCAAGACTT
TGATCCGGCAGGTATCGGTGCCCGCAGTTTACAAGAATGTCTTCTGCTACAAATACAAAGAAAAGCCGATTCACCGTTAA
AACAAATAGAACTGGACATCATCGGTAAATGCTGTGATGAGTTTACCCGCAAAAACAAAGAAAGGATTATCCAAAAACTA
GGTATTACCGAAGAACAATATAATGAGGCGGTGAGCGATCTCACCAAACTGAATCCTCGTCCGGGCAGTTCACTGGGTGA
AGCAATGGGCAAAAATATGCAGCAGATCATTCCCGACTTCATTGTAGAAACGTATGAGGATGACACCATTACTCTAAGTT
TAAACAATCGTAATGTTCCTGAGTTGCGTCTGAGCCGCCAGTTCACCGAACTACTGGATGAACACACCCGCAACAAAGAC
AATCAGAGCAAAGCCTCAAAGGACGCACTGATGTTCCTAAAACAAAAAGTAGATGCCGCACAAGGTTTTATCAACGCCGT
AAAACAGCGACAACATACGTTGCTCACCACCATGCAAGCCATTATAGATATTCAGCGCCCCTTCTTTTTGGAAGGAGACG
AATCCCTGCTAAAGCCCATGATCCTAAAAGATGTAGCCGAACGCTCGGGATTGGATATTTCCACCATATCACGGGTAAGC
AACAGCAAATATGTACAAACAAACTATGGTATCTATTCGTTGAAATTCTTTTTCAGCGACGGTTACACTACCGAAGACGG
TGAAGAACTCTCTGTCCGCGAAATCAAGCGTATTCTGAAAGAATGTGTAGACACCGAAGACAAGGAAAAACCGTACACTG
ATGATGAACTGGCCGAAACACTAAAAACGAAAGGCTACCCCATAGCCCGCCGTACTGTAGCCAAATACCGCCAACAGCTT
AACATACCCGTCGCACGATTAAGAAGGTAA

Upstream 100 bases:

>100_bases
GTAAAGATAGCCATCCCCTTAAAATAATCCCCCCAAAAAGGAAGATTATTACAATACAATTTCCTATTTTTGTCGCAATA
AACAAGGAAATAAAAATATT

Downstream 100 bases:

>100_bases
TATAAATTATATGGAAGCTAACGAAACAACCGAAACAATAGCAGTAGGCAAGAAAAACAGTCCGCTTGAAGTCAGTTCAC
GTATCATATCAGGCATATTC

Product: RNA polymerase sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MAQGSRQVQTQAQQQIQTLSPQQILVVKLLELPTVELEERIHSEILDNPALEEGKEMPENEDDNTEYVENEDGNTDSNED
FSLGDYSNEDDIPDYKLQEHNRSKEGVAEEIPFSDAVSFYEILKDQLQMQELTPEQRDIAEYLIGSLDDDGLLRKNMESI
MDELAIYRGIYTTEEELNKILEIIQDFDPAGIGARSLQECLLLQIQRKADSPLKQIELDIIGKCCDEFTRKNKERIIQKL
GITEEQYNEAVSDLTKLNPRPGSSLGEAMGKNMQQIIPDFIVETYEDDTITLSLNNRNVPELRLSRQFTELLDEHTRNKD
NQSKASKDALMFLKQKVDAAQGFINAVKQRQHTLLTTMQAIIDIQRPFFLEGDESLLKPMILKDVAERSGLDISTISRVS
NSKYVQTNYGIYSLKFFFSDGYTTEDGEELSVREIKRILKECVDTEDKEKPYTDDELAETLKTKGYPIARRTVAKYRQQL
NIPVARLRR

Sequences:

>Translated_489_residues
MAQGSRQVQTQAQQQIQTLSPQQILVVKLLELPTVELEERIHSEILDNPALEEGKEMPENEDDNTEYVENEDGNTDSNED
FSLGDYSNEDDIPDYKLQEHNRSKEGVAEEIPFSDAVSFYEILKDQLQMQELTPEQRDIAEYLIGSLDDDGLLRKNMESI
MDELAIYRGIYTTEEELNKILEIIQDFDPAGIGARSLQECLLLQIQRKADSPLKQIELDIIGKCCDEFTRKNKERIIQKL
GITEEQYNEAVSDLTKLNPRPGSSLGEAMGKNMQQIIPDFIVETYEDDTITLSLNNRNVPELRLSRQFTELLDEHTRNKD
NQSKASKDALMFLKQKVDAAQGFINAVKQRQHTLLTTMQAIIDIQRPFFLEGDESLLKPMILKDVAERSGLDISTISRVS
NSKYVQTNYGIYSLKFFFSDGYTTEDGEELSVREIKRILKECVDTEDKEKPYTDDELAETLKTKGYPIARRTVAKYRQQL
NIPVARLRR
>Mature_488_residues
AQGSRQVQTQAQQQIQTLSPQQILVVKLLELPTVELEERIHSEILDNPALEEGKEMPENEDDNTEYVENEDGNTDSNEDF
SLGDYSNEDDIPDYKLQEHNRSKEGVAEEIPFSDAVSFYEILKDQLQMQELTPEQRDIAEYLIGSLDDDGLLRKNMESIM
DELAIYRGIYTTEEELNKILEIIQDFDPAGIGARSLQECLLLQIQRKADSPLKQIELDIIGKCCDEFTRKNKERIIQKLG
ITEEQYNEAVSDLTKLNPRPGSSLGEAMGKNMQQIIPDFIVETYEDDTITLSLNNRNVPELRLSRQFTELLDEHTRNKDN
QSKASKDALMFLKQKVDAAQGFINAVKQRQHTLLTTMQAIIDIQRPFFLEGDESLLKPMILKDVAERSGLDISTISRVSN
SKYVQTNYGIYSLKFFFSDGYTTEDGEELSVREIKRILKECVDTEDKEKPYTDDELAETLKTKGYPIARRTVAKYRQQLN
IPVARLRR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=504, Percent_Identity=29.7619047619048, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 56142; Mature: 56011

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQGSRQVQTQAQQQIQTLSPQQILVVKLLELPTVELEERIHSEILDNPALEEGKEMPEN
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCC
EDDNTEYVENEDGNTDSNEDFSLGDYSNEDDIPDYKLQEHNRSKEGVAEEIPFSDAVSFY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHCCCHHHHHHH
EILKDQLQMQELTPEQRDIAEYLIGSLDDDGLLRKNMESIMDELAIYRGIYTTEEELNKI
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LEIIQDFDPAGIGARSLQECLLLQIQRKADSPLKQIELDIIGKCCDEFTRKNKERIIQKL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GITEEQYNEAVSDLTKLNPRPGSSLGEAMGKNMQQIIPDFIVETYEDDTITLSLNNRNVP
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCC
ELRLSRQFTELLDEHTRNKDNQSKASKDALMFLKQKVDAAQGFINAVKQRQHTLLTTMQA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIDIQRPFFLEGDESLLKPMILKDVAERSGLDISTISRVSNSKYVQTNYGIYSLKFFFSD
HHHHCCCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCEEEEEEEECC
GYTTEDGEELSVREIKRILKECVDTEDKEKPYTDDELAETLKTKGYPIARRTVAKYRQQL
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHC
NIPVARLRR
CCCHHHHCC
>Mature Secondary Structure 
AQGSRQVQTQAQQQIQTLSPQQILVVKLLELPTVELEERIHSEILDNPALEEGKEMPEN
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCC
EDDNTEYVENEDGNTDSNEDFSLGDYSNEDDIPDYKLQEHNRSKEGVAEEIPFSDAVSFY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHCCCHHHHHHH
EILKDQLQMQELTPEQRDIAEYLIGSLDDDGLLRKNMESIMDELAIYRGIYTTEEELNKI
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LEIIQDFDPAGIGARSLQECLLLQIQRKADSPLKQIELDIIGKCCDEFTRKNKERIIQKL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GITEEQYNEAVSDLTKLNPRPGSSLGEAMGKNMQQIIPDFIVETYEDDTITLSLNNRNVP
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCC
ELRLSRQFTELLDEHTRNKDNQSKASKDALMFLKQKVDAAQGFINAVKQRQHTLLTTMQA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIDIQRPFFLEGDESLLKPMILKDVAERSGLDISTISRVSNSKYVQTNYGIYSLKFFFSD
HHHHCCCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCEEEEEEEECC
GYTTEDGEELSVREIKRILKECVDTEDKEKPYTDDELAETLKTKGYPIARRTVAKYRQQL
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHC
NIPVARLRR
CCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2198257 [H]