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The map label for this gene is gcvH

Identifier: 150003983

GI number: 150003983

Start: 1893224

End: 1893604

Strand: Reverse

Name: gcvH

Synonym: BVU_1417

Alternate gene names: 150003983

Gene position: 1893604-1893224 (Counterclockwise)

Preceding gene: 150003984

Following gene: 150003982

Centisome position: 36.68

GC content: 38.58

Gene sequence:

>381_bases
ATGGAATTTCCAAGCAATGTAAAGTATACCAAAGAACACGAATGGATACGAGTAGAAGGTGATATAGCTTATGTAGGCAT
CACTGATTATGCACAAGAGCAACTGGGTGACATCGTATTCGTAGATATCACAACTGAAGGAGAAACTTTAGAAAAAGATG
AAGTTTTCGGTACTATTGAAGTAGTAAAAACGATTTCGGACTTGTTCCTTCCTGTTTCAGGAGAAGTTTTGGAACAGAAT
GAAGCATTGGCCGACAATCCCGAATTAGTAAACCAAGACCCGTATGGTAAAGGGTGGCTGATTAAGATTAAGCCCAATGA
CTCTAATGACGTTAACGATTTGCTGGATGCTGAAGGATATAAAGCATTAGTCAACGAATAA

Upstream 100 bases:

>100_bases
AGAAGTTTTAGCCGGATTTATTGTCGGATTGTTTTGTGGTGTCATTGGAATTTTGTTTATTTGAAAAAAATTAGTATTAA
CCTTATAAATTGAAAGCATT

Downstream 100 bases:

>100_bases
TATAGATTCCCATTAGGAATTGTTCAAGCGCGGATTACGCGGACCTCACGGATTTTTCTATCCGCGTTCATCCGTATAAC
CCGTGCTTGAAGTAAATATA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MEFPSNVKYTKEHEWIRVEGDIAYVGITDYAQEQLGDIVFVDITTEGETLEKDEVFGTIEVVKTISDLFLPVSGEVLEQN
EALADNPELVNQDPYGKGWLIKIKPNDSNDVNDLLDAEGYKALVNE

Sequences:

>Translated_126_residues
MEFPSNVKYTKEHEWIRVEGDIAYVGITDYAQEQLGDIVFVDITTEGETLEKDEVFGTIEVVKTISDLFLPVSGEVLEQN
EALADNPELVNQDPYGKGWLIKIKPNDSNDVNDLLDAEGYKALVNE
>Mature_126_residues
MEFPSNVKYTKEHEWIRVEGDIAYVGITDYAQEQLGDIVFVDITTEGETLEKDEVFGTIEVVKTISDLFLPVSGEVLEQN
EALADNPELVNQDPYGKGWLIKIKPNDSNDVNDLLDAEGYKALVNE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=113, Percent_Identity=46.0176991150442, Blast_Score=113, Evalue=5e-26,
Organism=Homo sapiens, GI89057342, Length=113, Percent_Identity=45.1327433628319, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1789271, Length=122, Percent_Identity=50.8196721311475, Blast_Score=130, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17551294, Length=127, Percent_Identity=43.3070866141732, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=43.75, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=42.1875, Blast_Score=99, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=42.0168067226891, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_BACV8 (A6L091)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298727.1
- ProteinModelPortal:   A6L091
- SMR:   A6L091
- STRING:   A6L091
- GeneID:   5302383
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1417
- NMPDR:   fig|435590.6.peg.1340
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14151; Mature: 14151

Theoretical pI: Translated: 3.78; Mature: 3.78

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFPSNVKYTKEHEWIRVEGDIAYVGITDYAQEQLGDIVFVDITTEGETLEKDEVFGTIE
CCCCCCCCCCCCCCEEEEECCEEEEEECHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHH
VVKTISDLFLPVSGEVLEQNEALADNPELVNQDPYGKGWLIKIKPNDSNDVNDLLDAEGY
HHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCC
KALVNE
EEECCC
>Mature Secondary Structure
MEFPSNVKYTKEHEWIRVEGDIAYVGITDYAQEQLGDIVFVDITTEGETLEKDEVFGTIE
CCCCCCCCCCCCCCEEEEECCEEEEEECHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHH
VVKTISDLFLPVSGEVLEQNEALADNPELVNQDPYGKGWLIKIKPNDSNDVNDLLDAEGY
HHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCC
KALVNE
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA