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The map label for this gene is ispG

Identifier: 150003981

GI number: 150003981

Start: 1890662

End: 1892506

Strand: Reverse

Name: ispG

Synonym: BVU_1415

Alternate gene names: 150003981

Gene position: 1892506-1890662 (Counterclockwise)

Preceding gene: 150003982

Following gene: 150003980

Centisome position: 36.65

GC content: 46.4

Gene sequence:

>1845_bases
ATGGATTTATTCAATTACTCCCGCCGCGAATCTTCCGAAGTGAATATCGGAGCCACTCCCTTGGGTGGCAACAACCCCAT
CCGTATTCAGAGCATGACAAATACCGTCACTATGGATACCGAAGCCTGTGTGGAACAAGCCAAACGCATTATAGATGCCG
GCGGTGAATACGTTCGCCTCACCACACAAGGTGTCCGTGAAGCCGAAAACTTGAAGAATATCAATATCGGATTACGCTCT
CAAGGATATGATACTCCATTGGTGGCCGATGTACATTTTAACCCCAAAGTAGCCGATGTAGCGGCACAATATGCAGAAAA
GGTACGCATCAATCCGGGAAATTACGTAGACCCGGGACGTACTTTCCAGAAACTGGAATACACTGACGAAGAATATGCGC
AAGAAATAGAAAAGATACGTGCCCGCTTCATTCCTTTTCTAAATATCTGCAAGGAAAACCACACCGCCATCCGCATCGGG
GTAAACCACGGTTCTCTGTCAGACCGCATCATGTCTCATTATGGTGACACCCCTGAAGGTATGGTGGAATCGTGCATGGA
ATTTCTGCGTATCTGTGTTGCGGAACATTTCAACGATGTGGTTATTTCCATCAAAGCATCCAATACCGTAGTAATGGTCA
GGACGGTCCGTCTGCTGGTAAAGGAAATGGAAAAGGAAGGCATGGCTTTCCCGCTTCATTTGGGGGTTACCGAAGCAGGG
GACGGCGAGGACGGAAGAATAAAATCCGCATTAGGAATAGGCGCACTCCTTGCAGATGGCTTGGGAGATACCATTCGTGT
TTCATTGAGCGAGGCTCCTGAAAACGAAATTCCGGTAGCCCGCAAACTGGTTGATTATATACTAACTCGGGAAGGACATC
CTTTTATTCCTGGAAAAGAAGCTCCGCAGTTCAACTACCTATCTCCGGGGCGCCGGAAGACTAAGGCTGTCCGAAACATT
GGAGGAGATAATCTTCCCGTAGTAATAGCCGAACGTCTGGAAGGGTCTTTCGAAACTAATCCTCAGTTCAAACCTGACTA
TATTTATTGCGGTGGAAGTGTGCCCCAATCACGAGATAACAATATAGCCTATCTAGTAGATGCCAATGCATGGAATCCAG
AAGACAAAAATGTCTATCCGGCATTTAACTACCAGCAGATGATAGAATTACATCACACGGTTTCCGATCTGAAATTCCTG
TTTCTGCCCTATATGGCTATGAATGACGAAGTAATCGCAGCCCTTAAACTACATCCCGAAGTGGTAATCATCGCCCAAAG
CAATCATCCCAACCGCCTCGGCGAATATCGTGCCATGACACACGAACTGATGAATGAAGGGTTAGAGAATCCGGTCGTAT
TCTTCCAATACTATCAGGAAACGAAAGCTGAAGACCTGCAAATCAAAGCGGCAGCTGATATGGGTGCACTAATTTTTGAC
GGACTCTGTGACGGAATCTTCTTGTACAACCAAGGCCCCTTGAGTCATATAGTGGTAGATACCACTGCATTTGGTATCCT
GCAAGCCGGCAGAATCCGAACCAGCAAAACGGAATACATTTCATGTCCGGGATGTGGTCGTACCTTATATGATTTGGAGT
CTACCATCGCCCGTATCAAAACAGCTACCTCGCACTTGAAAGGTCTGAAGATCGGCATTATGGGCTGTATTGTCAATGGT
CCCGGCGAAATGGCGGATGCCGACTATGGCTATGTAGGTGCCGGTCGTGGAAAAATCAGCCTCTATAAAAAGAAAGAATG
CATAGAAAAAAACATTCCCGAAGATCAGGCTGTTGAGAAGTTGATCGAGCTCATCAAAGCCAATGGTGACTATACAGAAA
AATAA

Upstream 100 bases:

>100_bases
AATATAAAACAAATTAACTAGTGGTTAGTGATTAGTGGTAAGTGGTTAATATTTCAGCGCAGCCCACTAATCACTAACCA
CTCACCACTAATCACTAACT

Downstream 100 bases:

>100_bases
AACGTCCAAATTATGGATTATCAACAGATTCTAAAAGACATCTATCAAGAAATCCAGCCCTACGCCTCTATTGGCAAACA
GGCTGATTACATTCCGGCAC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MDLFNYSRRESSEVNIGATPLGGNNPIRIQSMTNTVTMDTEACVEQAKRIIDAGGEYVRLTTQGVREAENLKNINIGLRS
QGYDTPLVADVHFNPKVADVAAQYAEKVRINPGNYVDPGRTFQKLEYTDEEYAQEIEKIRARFIPFLNICKENHTAIRIG
VNHGSLSDRIMSHYGDTPEGMVESCMEFLRICVAEHFNDVVISIKASNTVVMVRTVRLLVKEMEKEGMAFPLHLGVTEAG
DGEDGRIKSALGIGALLADGLGDTIRVSLSEAPENEIPVARKLVDYILTREGHPFIPGKEAPQFNYLSPGRRKTKAVRNI
GGDNLPVVIAERLEGSFETNPQFKPDYIYCGGSVPQSRDNNIAYLVDANAWNPEDKNVYPAFNYQQMIELHHTVSDLKFL
FLPYMAMNDEVIAALKLHPEVVIIAQSNHPNRLGEYRAMTHELMNEGLENPVVFFQYYQETKAEDLQIKAAADMGALIFD
GLCDGIFLYNQGPLSHIVVDTTAFGILQAGRIRTSKTEYISCPGCGRTLYDLESTIARIKTATSHLKGLKIGIMGCIVNG
PGEMADADYGYVGAGRGKISLYKKKECIEKNIPEDQAVEKLIELIKANGDYTEK

Sequences:

>Translated_614_residues
MDLFNYSRRESSEVNIGATPLGGNNPIRIQSMTNTVTMDTEACVEQAKRIIDAGGEYVRLTTQGVREAENLKNINIGLRS
QGYDTPLVADVHFNPKVADVAAQYAEKVRINPGNYVDPGRTFQKLEYTDEEYAQEIEKIRARFIPFLNICKENHTAIRIG
VNHGSLSDRIMSHYGDTPEGMVESCMEFLRICVAEHFNDVVISIKASNTVVMVRTVRLLVKEMEKEGMAFPLHLGVTEAG
DGEDGRIKSALGIGALLADGLGDTIRVSLSEAPENEIPVARKLVDYILTREGHPFIPGKEAPQFNYLSPGRRKTKAVRNI
GGDNLPVVIAERLEGSFETNPQFKPDYIYCGGSVPQSRDNNIAYLVDANAWNPEDKNVYPAFNYQQMIELHHTVSDLKFL
FLPYMAMNDEVIAALKLHPEVVIIAQSNHPNRLGEYRAMTHELMNEGLENPVVFFQYYQETKAEDLQIKAAADMGALIFD
GLCDGIFLYNQGPLSHIVVDTTAFGILQAGRIRTSKTEYISCPGCGRTLYDLESTIARIKTATSHLKGLKIGIMGCIVNG
PGEMADADYGYVGAGRGKISLYKKKECIEKNIPEDQAVEKLIELIKANGDYTEK
>Mature_614_residues
MDLFNYSRRESSEVNIGATPLGGNNPIRIQSMTNTVTMDTEACVEQAKRIIDAGGEYVRLTTQGVREAENLKNINIGLRS
QGYDTPLVADVHFNPKVADVAAQYAEKVRINPGNYVDPGRTFQKLEYTDEEYAQEIEKIRARFIPFLNICKENHTAIRIG
VNHGSLSDRIMSHYGDTPEGMVESCMEFLRICVAEHFNDVVISIKASNTVVMVRTVRLLVKEMEKEGMAFPLHLGVTEAG
DGEDGRIKSALGIGALLADGLGDTIRVSLSEAPENEIPVARKLVDYILTREGHPFIPGKEAPQFNYLSPGRRKTKAVRNI
GGDNLPVVIAERLEGSFETNPQFKPDYIYCGGSVPQSRDNNIAYLVDANAWNPEDKNVYPAFNYQQMIELHHTVSDLKFL
FLPYMAMNDEVIAALKLHPEVVIIAQSNHPNRLGEYRAMTHELMNEGLENPVVFFQYYQETKAEDLQIKAAADMGALIFD
GLCDGIFLYNQGPLSHIVVDTTAFGILQAGRIRTSKTEYISCPGCGRTLYDLESTIARIKTATSHLKGLKIGIMGCIVNG
PGEMADADYGYVGAGRGKISLYKKKECIEKNIPEDQAVEKLIELIKANGDYTEK

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=292, Percent_Identity=36.6438356164384, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_BACV8 (A6L089)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298725.1
- STRING:   A6L089
- GeneID:   5302381
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1415
- NMPDR:   fig|435590.6.peg.1338
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   SMRIGTN
- ProtClustDB:   PRK02048
- HAMAP:   MF_00159
- InterPro:   IPR017178
- InterPro:   IPR004588
- PIRSF:   PIRSF037336
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 68160; Mature: 68160

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLFNYSRRESSEVNIGATPLGGNNPIRIQSMTNTVTMDTEACVEQAKRIIDAGGEYVRL
CCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCEEEECHHHHHHHHHHHHHCCCCEEEE
TTQGVREAENLKNINIGLRSQGYDTPLVADVHFNPKVADVAAQYAEKVRINPGNYVDPGR
EHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHEECCCCCCCCCCC
TFQKLEYTDEEYAQEIEKIRARFIPFLNICKENHTAIRIGVNHGSLSDRIMSHYGDTPEG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCHHH
MVESCMEFLRICVAEHFNDVVISIKASNTVVMVRTVRLLVKEMEKEGMAFPLHLGVTEAG
HHHHHHHHHHHHHHHCCCEEEEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCC
DGEDGRIKSALGIGALLADGLGDTIRVSLSEAPENEIPVARKLVDYILTREGHPFIPGKE
CCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
APQFNYLSPGRRKTKAVRNIGGDNLPVVIAERLEGSFETNPQFKPDYIYCGGSVPQSRDN
CCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCC
NIAYLVDANAWNPEDKNVYPAFNYQQMIELHHTVSDLKFLFLPYMAMNDEVIAALKLHPE
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCE
VVIIAQSNHPNRLGEYRAMTHELMNEGLENPVVFFQYYQETKAEDLQIKAAADMGALIFD
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCCEEEEECCCCHHHHHH
GLCDGIFLYNQGPLSHIVVDTTAFGILQAGRIRTSKTEYISCPGCGRTLYDLESTIARIK
HHHCCEEEECCCCCCEEEEEHHHHHHHHCCCEECCCCCEEECCCCCCHHHHHHHHHHHHH
TATSHLKGLKIGIMGCIVNGPGEMADADYGYVGAGRGKISLYKKKECIEKNIPEDQAVEK
HHHHHHCCCEEEEEEEEECCCCCCCCCCCCEEECCCCEEEEHHHHHHHHCCCCHHHHHHH
LIELIKANGDYTEK
HHHHHHCCCCCCCC
>Mature Secondary Structure
MDLFNYSRRESSEVNIGATPLGGNNPIRIQSMTNTVTMDTEACVEQAKRIIDAGGEYVRL
CCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCEEEECHHHHHHHHHHHHHCCCCEEEE
TTQGVREAENLKNINIGLRSQGYDTPLVADVHFNPKVADVAAQYAEKVRINPGNYVDPGR
EHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHEECCCCCCCCCCC
TFQKLEYTDEEYAQEIEKIRARFIPFLNICKENHTAIRIGVNHGSLSDRIMSHYGDTPEG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCHHH
MVESCMEFLRICVAEHFNDVVISIKASNTVVMVRTVRLLVKEMEKEGMAFPLHLGVTEAG
HHHHHHHHHHHHHHHCCCEEEEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCC
DGEDGRIKSALGIGALLADGLGDTIRVSLSEAPENEIPVARKLVDYILTREGHPFIPGKE
CCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
APQFNYLSPGRRKTKAVRNIGGDNLPVVIAERLEGSFETNPQFKPDYIYCGGSVPQSRDN
CCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCC
NIAYLVDANAWNPEDKNVYPAFNYQQMIELHHTVSDLKFLFLPYMAMNDEVIAALKLHPE
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCE
VVIIAQSNHPNRLGEYRAMTHELMNEGLENPVVFFQYYQETKAEDLQIKAAADMGALIFD
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCCEEEEECCCCHHHHHH
GLCDGIFLYNQGPLSHIVVDTTAFGILQAGRIRTSKTEYISCPGCGRTLYDLESTIARIK
HHHCCEEEECCCCCCEEEEEHHHHHHHHCCCEECCCCCEEECCCCCCHHHHHHHHHHHHH
TATSHLKGLKIGIMGCIVNGPGEMADADYGYVGAGRGKISLYKKKECIEKNIPEDQAVEK
HHHHHHCCCEEEEEEEEECCCCCCCCCCCCEEECCCCEEEEHHHHHHHHCCCCHHHHHHH
LIELIKANGDYTEK
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA