Click here to switch to the map view.

The map label for this gene is mraW

Identifier: 150003970

GI number: 150003970

Start: 1878923

End: 1879837

Strand: Reverse

Name: mraW

Synonym: BVU_1404

Alternate gene names: 150003970

Gene position: 1879837-1878923 (Counterclockwise)

Preceding gene: 150003971

Following gene: 150003969

Centisome position: 36.41

GC content: 45.03

Gene sequence:

>915_bases
ATGAGTGAAGCAAAACAGATATATCATGTACCGGTATTATTAAACGAGAGCGTAGACGGAATGAACATCCAACCCGGAGG
TATTTATGTAGATGCCACTTTCGGCGGCGGAGGACATTCCAAGGAAATTCTAAGCCGGCTGGATAGCACCGCGCATCTAT
ATAGTTTTGACCAAGATGAAGATGCCGAGAAGAATATTGTAAGCGACAGCCGCTTCACTTTTGTGCGTAGTAACTTCCGC
TATCTTCCCAACTTCCTACGTTATTATGGCGTGGAAGGGGTGGATGCCATTTTGGCCGATCTGGGCGTTTCCTCACATCA
CTTTGACGACTCCGAACGTGGATTCTCATTCCGCTTCGAGGGCAAATTAGACATGCGGATGAACAAACGCGCAGGTATGA
CAGCTGCTGATGTAGTGAACACCTATGACGAAGAACGTTTGGCTAATATTTTCTATTTATATGGTGAATTGAAGAACAGC
CGCAAACTGGCATCAGCCATCGTTAAAGCACGAGGTGTAAAACAAATTGTCACCATCGGTGATTTCCTCGAAGTCATCAA
ACCGCTTTTCGGACGGGAACGTGAAAAGAAAGAGCTCGCAAAGGTGTTCCAAGCTTTACGCATAGAGGTAAATCAGGAAA
TGGAAGCATTGAAAGAAATGCTTTATGCAGCAACCAAGGCACTGAAACCGGGAGGCAGACTGGTAGTAATCACCTATCAC
TCTTTAGAAGACCGCATGGTAAAGAATATCATGAAAACCGGAAACATAGAAGGCAAGGCCGAACAAGATTTCTTTGGTAA
TGTACAGACACCTTTCAAACTAGTCAACAACAAAGTTATTGTTGCCGGCAACGAAGAAGTGACACGCAATCCGCGCTCCC
GAAGCGCCAAGTTGAGAATTGCAGAAAAGAGATAA

Upstream 100 bases:

>100_bases
TCTGGTCTAAAGAAATAGCAGACAAACCCTTCATCACACCGGAGGACTTTGGAAAAGAATTGGAAGAAATTATGGGAACT
AATAACAACGTAGAAATAGA

Downstream 100 bases:

>100_bases
CGAGATGGAAGAAGAACTGAAACAAAATACACAACAACCCAAAGACGGACAATCTGCTTCGCCCAAGCATATGTCTATCC
GCAGCATATTAGGAGGAGAT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSEAKQIYHVPVLLNESVDGMNIQPGGIYVDATFGGGGHSKEILSRLDSTAHLYSFDQDEDAEKNIVSDSRFTFVRSNFR
YLPNFLRYYGVEGVDAILADLGVSSHHFDDSERGFSFRFEGKLDMRMNKRAGMTAADVVNTYDEERLANIFYLYGELKNS
RKLASAIVKARGVKQIVTIGDFLEVIKPLFGREREKKELAKVFQALRIEVNQEMEALKEMLYAATKALKPGGRLVVITYH
SLEDRMVKNIMKTGNIEGKAEQDFFGNVQTPFKLVNNKVIVAGNEEVTRNPRSRSAKLRIAEKR

Sequences:

>Translated_304_residues
MSEAKQIYHVPVLLNESVDGMNIQPGGIYVDATFGGGGHSKEILSRLDSTAHLYSFDQDEDAEKNIVSDSRFTFVRSNFR
YLPNFLRYYGVEGVDAILADLGVSSHHFDDSERGFSFRFEGKLDMRMNKRAGMTAADVVNTYDEERLANIFYLYGELKNS
RKLASAIVKARGVKQIVTIGDFLEVIKPLFGREREKKELAKVFQALRIEVNQEMEALKEMLYAATKALKPGGRLVVITYH
SLEDRMVKNIMKTGNIEGKAEQDFFGNVQTPFKLVNNKVIVAGNEEVTRNPRSRSAKLRIAEKR
>Mature_303_residues
SEAKQIYHVPVLLNESVDGMNIQPGGIYVDATFGGGGHSKEILSRLDSTAHLYSFDQDEDAEKNIVSDSRFTFVRSNFRY
LPNFLRYYGVEGVDAILADLGVSSHHFDDSERGFSFRFEGKLDMRMNKRAGMTAADVVNTYDEERLANIFYLYGELKNSR
KLASAIVKARGVKQIVTIGDFLEVIKPLFGREREKKELAKVFQALRIEVNQEMEALKEMLYAATKALKPGGRLVVITYHS
LEDRMVKNIMKTGNIEGKAEQDFFGNVQTPFKLVNNKVIVAGNEEVTRNPRSRSAKLRIAEKR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=36.9047619047619, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=38.8297872340425, Blast_Score=121, Evalue=7e-28,
Organism=Escherichia coli, GI1786270, Length=307, Percent_Identity=38.7622149837134, Blast_Score=206, Evalue=2e-54,
Organism=Drosophila melanogaster, GI62472493, Length=329, Percent_Identity=35.5623100303951, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI28571637, Length=329, Percent_Identity=35.5623100303951, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_BACV8 (A6L078)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298714.1
- ProteinModelPortal:   A6L078
- SMR:   A6L078
- STRING:   A6L078
- GeneID:   5302370
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1404
- NMPDR:   fig|435590.6.peg.1326
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34390; Mature: 34259

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: BINDING 57-57 BINDING 79-79 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAKQIYHVPVLLNESVDGMNIQPGGIYVDATFGGGGHSKEILSRLDSTAHLYSFDQDE
CCCCCCEEEEEEEEECCCCCCEECCCCEEEEEEECCCCCHHHHHHHHCCHHHEEECCCCC
DAEKNIVSDSRFTFVRSNFRYLPNFLRYYGVEGVDAILADLGVSSHHFDDSERGFSFRFE
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEC
GKLDMRMNKRAGMTAADVVNTYDEERLANIFYLYGELKNSRKLASAIVKARGVKQIVTIG
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
DFLEVIKPLFGREREKKELAKVFQALRIEVNQEMEALKEMLYAATKALKPGGRLVVITYH
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
SLEDRMVKNIMKTGNIEGKAEQDFFGNVQTPFKLVNNKVIVAGNEEVTRNPRSRSAKLRI
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCEEEEECCHHHHCCCCCCCCEEEE
AEKR
ECCC
>Mature Secondary Structure 
SEAKQIYHVPVLLNESVDGMNIQPGGIYVDATFGGGGHSKEILSRLDSTAHLYSFDQDE
CCCCCEEEEEEEEECCCCCCEECCCCEEEEEEECCCCCHHHHHHHHCCHHHEEECCCCC
DAEKNIVSDSRFTFVRSNFRYLPNFLRYYGVEGVDAILADLGVSSHHFDDSERGFSFRFE
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEC
GKLDMRMNKRAGMTAADVVNTYDEERLANIFYLYGELKNSRKLASAIVKARGVKQIVTIG
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
DFLEVIKPLFGREREKKELAKVFQALRIEVNQEMEALKEMLYAATKALKPGGRLVVITYH
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
SLEDRMVKNIMKTGNIEGKAEQDFFGNVQTPFKLVNNKVIVAGNEEVTRNPRSRSAKLRI
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCEEEEECCHHHHCCCCCCCCEEEE
AEKR
ECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA