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The map label for this gene is gyrB [H]

Identifier: 150003955

GI number: 150003955

Start: 1859937

End: 1861901

Strand: Reverse

Name: gyrB [H]

Synonym: BVU_1388

Alternate gene names: 150003955

Gene position: 1861901-1859937 (Counterclockwise)

Preceding gene: 150003956

Following gene: 150003954

Centisome position: 36.06

GC content: 46.87

Gene sequence:

>1965_bases
ATGAGTGAAGAAGAAAAGATAAACGGCGAAAACAATTACTCGGCCAGTAACATCCAGGTATTGGAAGGTTTGGAGGCTGT
ACGTAAACGTCCCGCCATGTATATCGGAGACATCAGCGAAAAGGGATTGCACCACTTGGTTTACGAGGTTGTGGACAACT
CTATCGACGAGGCATTGGCCGGCTACTGTGATCATATAGAAGTCACCATCAATGAAGACAACTCCATTACCGTACAGGAT
AATGGCCGTGGTATCCCGGTAGACTTTCACGAAAAAGAAAAGAAATCTGCATTGGAAGTGGTAATGACCGTGCTGCACGC
GGGAGGTAAGTTCGACAAAGGTTCTTACAAGGTTTCAGGCGGTCTGCACGGTGTAGGTGTTTCTTGTGTGAACGCCTTGT
CTACTCACATGACCACACAGGTATTCCGCGGTGGCAAGATCTACCAGCAGGAATACAGCTGCGGACATCCTTTGTATTCT
GTAAAAGAAGTAGGAACAGCTGATATTACCGGAACAAAACAGACTTTCTGGCCGGATGATACCATCTTCACTGTTACCGA
ATATAAGTTTGACATTCTACAGGCACGTATGCGTGAATTGGCCTACTTGAACAAAGGTATCACCATTTCACTGACCGACC
GCCGGATCAAAGAAGAAGATGGCAGCTTCAAGAAAGAAATATTCCATTCGGACGAAGGAGTGAAAGAGTTTGTACGTTTC
CTGAACCGTAACAACGAAGCGCTGATTAATGATGTCATTTATCTGAATACCGAAAAAAACAATACCCCCATTGAATGTGC
CATCATGTACAATACAGGCTATCGTGAAAGCCTGCATTCGTATGTAAACAATATCAATACAATAGAAGGCGGTACACACG
AGGCCGGTTTCCGCAGCGCATTAACCCGTGTACTGAAGAAATATGCGGAAGATACCAAAGCACTGGAAAAAGCAAAAGTC
GAGATTTCGGGAGAGGACTTCCGCGAAGGCTTGATTGCCGTCATTTCAGTGAAAGTAGCCGAGCCGCAGTTCGAAGGACA
GACCAAGACCAAGCTGGGCAACAGCGAAGTGAGTGGTGCCGTGAACCAAGCTGTAGGCGAAGCGCTTACATATTATCTGG
AAGAACATCCGAAAGAAGCAAAACAGATTGTTGACAAAGTGATCCTGGCTGCAACAGCGCGTATCGCCGCACGCAAGGCA
CGTGAATCTGTTCAAAGAAAGAGTCCGATGGGCGGTGGCGGACTGCCGGGCAAACTGGCCGACTGCTCGAGCCGTAATCC
GGAGGAATGTGAACTATTCCTGGTCGAGGGTGACTCGGCAGGTGGTTCTGCCAAGCAAGGACGTAGCCGTGCCTTCCAGG
CAATTCTACCTTTGAGGGGTAAAATCCTGAATGTGGAAAAAGCGATGTGGCACAAGGCTTTTGAAAGCGATGAGGTCAAT
AATATCATCACCGCCCTGGGTGTCCGTTTCGGTGTGGACGGAAATGATGACAGCAAAAAAGCGAACATCGACAAGCTGCG
TTATCACAAAGTGGTGATCATGACCGATGCCGATGTCGATGGTTCTCACATCGACACCCTTATCATGACATTGTTCTACC
GCTATATGCCGGAAATTATAGAAAACGGACATTTGTACATTGCCAATCCGCCATTGTATAAATGCAGCAAAGGAAAAATC
AGTGAATACTGTTACACCGAAGAAGCACGCCAGGCATTCATTCAAAAATATGGTGACGGACAAGAAAACGGAATCCATAC
ACAGCGATACAAAGGTTTGGGTGAGATGAATCCCGAACAACTGTGGGAAACAACAATGAACCCTGAAACGCGTATTCTGA
AACAGGTAACAATTGACAATGCCGCCAACGTAGACTATATCTTCTCCATGCTGATGGGAGACGATGTAGCTCCACGCCGC
GAGTTCATAGAGAAAAACGCGACATATGCGAATATAGACGCGTAA

Upstream 100 bases:

>100_bases
CCAATCCCCTCTTTTTTACGTTTTTTAGTCCACAAAAGCTGCCTGTCTCAGCAATTTTTCGTATCTTTGAAAGCTAATAT
TTAAGATAATTGAATCAAAC

Downstream 100 bases:

>100_bases
TTTTTCATTCATTATTTTTTAATCAAAATCCATCCTCACATCTTACAATTTGAGGTAGCCCCGATCCTGCTTTTCTCTGG
ATCGGGGCTTTTTTATCAAT

Product: DNA gyrase B subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 654; Mature: 653

Protein sequence:

>654_residues
MSEEEKINGENNYSASNIQVLEGLEAVRKRPAMYIGDISEKGLHHLVYEVVDNSIDEALAGYCDHIEVTINEDNSITVQD
NGRGIPVDFHEKEKKSALEVVMTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSTHMTTQVFRGGKIYQQEYSCGHPLYS
VKEVGTADITGTKQTFWPDDTIFTVTEYKFDILQARMRELAYLNKGITISLTDRRIKEEDGSFKKEIFHSDEGVKEFVRF
LNRNNEALINDVIYLNTEKNNTPIECAIMYNTGYRESLHSYVNNINTIEGGTHEAGFRSALTRVLKKYAEDTKALEKAKV
EISGEDFREGLIAVISVKVAEPQFEGQTKTKLGNSEVSGAVNQAVGEALTYYLEEHPKEAKQIVDKVILAATARIAARKA
RESVQRKSPMGGGGLPGKLADCSSRNPEECELFLVEGDSAGGSAKQGRSRAFQAILPLRGKILNVEKAMWHKAFESDEVN
NIITALGVRFGVDGNDDSKKANIDKLRYHKVVIMTDADVDGSHIDTLIMTLFYRYMPEIIENGHLYIANPPLYKCSKGKI
SEYCYTEEARQAFIQKYGDGQENGIHTQRYKGLGEMNPEQLWETTMNPETRILKQVTIDNAANVDYIFSMLMGDDVAPRR
EFIEKNATYANIDA

Sequences:

>Translated_654_residues
MSEEEKINGENNYSASNIQVLEGLEAVRKRPAMYIGDISEKGLHHLVYEVVDNSIDEALAGYCDHIEVTINEDNSITVQD
NGRGIPVDFHEKEKKSALEVVMTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSTHMTTQVFRGGKIYQQEYSCGHPLYS
VKEVGTADITGTKQTFWPDDTIFTVTEYKFDILQARMRELAYLNKGITISLTDRRIKEEDGSFKKEIFHSDEGVKEFVRF
LNRNNEALINDVIYLNTEKNNTPIECAIMYNTGYRESLHSYVNNINTIEGGTHEAGFRSALTRVLKKYAEDTKALEKAKV
EISGEDFREGLIAVISVKVAEPQFEGQTKTKLGNSEVSGAVNQAVGEALTYYLEEHPKEAKQIVDKVILAATARIAARKA
RESVQRKSPMGGGGLPGKLADCSSRNPEECELFLVEGDSAGGSAKQGRSRAFQAILPLRGKILNVEKAMWHKAFESDEVN
NIITALGVRFGVDGNDDSKKANIDKLRYHKVVIMTDADVDGSHIDTLIMTLFYRYMPEIIENGHLYIANPPLYKCSKGKI
SEYCYTEEARQAFIQKYGDGQENGIHTQRYKGLGEMNPEQLWETTMNPETRILKQVTIDNAANVDYIFSMLMGDDVAPRR
EFIEKNATYANIDA
>Mature_653_residues
SEEEKINGENNYSASNIQVLEGLEAVRKRPAMYIGDISEKGLHHLVYEVVDNSIDEALAGYCDHIEVTINEDNSITVQDN
GRGIPVDFHEKEKKSALEVVMTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSTHMTTQVFRGGKIYQQEYSCGHPLYSV
KEVGTADITGTKQTFWPDDTIFTVTEYKFDILQARMRELAYLNKGITISLTDRRIKEEDGSFKKEIFHSDEGVKEFVRFL
NRNNEALINDVIYLNTEKNNTPIECAIMYNTGYRESLHSYVNNINTIEGGTHEAGFRSALTRVLKKYAEDTKALEKAKVE
ISGEDFREGLIAVISVKVAEPQFEGQTKTKLGNSEVSGAVNQAVGEALTYYLEEHPKEAKQIVDKVILAATARIAARKAR
ESVQRKSPMGGGGLPGKLADCSSRNPEECELFLVEGDSAGGSAKQGRSRAFQAILPLRGKILNVEKAMWHKAFESDEVNN
IITALGVRFGVDGNDDSKKANIDKLRYHKVVIMTDADVDGSHIDTLIMTLFYRYMPEIIENGHLYIANPPLYKCSKGKIS
EYCYTEEARQAFIQKYGDGQENGIHTQRYKGLGEMNPEQLWETTMNPETRILKQVTIDNAANVDYIFSMLMGDDVAPRRE
FIEKNATYANIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=624, Percent_Identity=26.9230769230769, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI19913406, Length=625, Percent_Identity=25.12, Blast_Score=138, Evalue=2e-32,
Organism=Escherichia coli, GI48994957, Length=565, Percent_Identity=55.2212389380531, Blast_Score=589, Evalue=1e-169,
Organism=Escherichia coli, GI1789408, Length=649, Percent_Identity=38.0585516178737, Blast_Score=418, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17535065, Length=627, Percent_Identity=25.3588516746411, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI212645845, Length=563, Percent_Identity=24.6891651865009, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=154, Percent_Identity=33.7662337662338, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=694, Percent_Identity=25.5043227665706, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17136538, Length=579, Percent_Identity=25.73402417962, Blast_Score=142, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72966; Mature: 72835

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEEKINGENNYSASNIQVLEGLEAVRKRPAMYIGDISEKGLHHLVYEVVDNSIDEALA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCHHHHHHH
GYCDHIEVTINEDNSITVQDNGRGIPVDFHEKEKKSALEVVMTVLHAGGKFDKGSYKVSG
CCCCEEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGVSCVNALSTHMTTQVFRGGKIYQQEYSCGHPLYSVKEVGTADITGTKQTFWPDD
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCC
TIFTVTEYKFDILQARMRELAYLNKGITISLTDRRIKEEDGSFKKEIFHSDEGVKEFVRF
CEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCHHHHHHCCCCCHHHHHHH
LNRNNEALINDVIYLNTEKNNTPIECAIMYNTGYRESLHSYVNNINTIEGGTHEAGFRSA
HCCCCCEEEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHH
LTRVLKKYAEDTKALEKAKVEISGEDFREGLIAVISVKVAEPQFEGQTKTKLGNSEVSGA
HHHHHHHHHHHHHHHHHHEEEECCHHHHCCCEEEEEEEEECCCCCCCHHHCCCCHHHHHH
VNQAVGEALTYYLEEHPKEAKQIVDKVILAATARIAARKARESVQRKSPMGGGGLPGKLA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DCSSRNPEECELFLVEGDSAGGSAKQGRSRAFQAILPLRGKILNVEKAMWHKAFESDEVN
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHHHCCCCHHH
NIITALGVRFGVDGNDDSKKANIDKLRYHKVVIMTDADVDGSHIDTLIMTLFYRYMPEII
HHHHHHHHEECCCCCCCCCCCCHHHEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
ENGHLYIANPPLYKCSKGKISEYCYTEEARQAFIQKYGDGQENGIHTQRYKGLGEMNPEQ
HCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHH
LWETTMNPETRILKQVTIDNAANVDYIFSMLMGDDVAPRREFIEKNATYANIDA
HHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
SEEEKINGENNYSASNIQVLEGLEAVRKRPAMYIGDISEKGLHHLVYEVVDNSIDEALA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCHHHHHHH
GYCDHIEVTINEDNSITVQDNGRGIPVDFHEKEKKSALEVVMTVLHAGGKFDKGSYKVSG
CCCCEEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGVSCVNALSTHMTTQVFRGGKIYQQEYSCGHPLYSVKEVGTADITGTKQTFWPDD
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCC
TIFTVTEYKFDILQARMRELAYLNKGITISLTDRRIKEEDGSFKKEIFHSDEGVKEFVRF
CEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCHHHHHHCCCCCHHHHHHH
LNRNNEALINDVIYLNTEKNNTPIECAIMYNTGYRESLHSYVNNINTIEGGTHEAGFRSA
HCCCCCEEEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHH
LTRVLKKYAEDTKALEKAKVEISGEDFREGLIAVISVKVAEPQFEGQTKTKLGNSEVSGA
HHHHHHHHHHHHHHHHHHEEEECCHHHHCCCEEEEEEEEECCCCCCCHHHCCCCHHHHHH
VNQAVGEALTYYLEEHPKEAKQIVDKVILAATARIAARKARESVQRKSPMGGGGLPGKLA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DCSSRNPEECELFLVEGDSAGGSAKQGRSRAFQAILPLRGKILNVEKAMWHKAFESDEVN
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHHHCCCCHHH
NIITALGVRFGVDGNDDSKKANIDKLRYHKVVIMTDADVDGSHIDTLIMTLFYRYMPEII
HHHHHHHHEECCCCCCCCCCCCHHHEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
ENGHLYIANPPLYKCSKGKISEYCYTEEARQAFIQKYGDGQENGIHTQRYKGLGEMNPEQ
HCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHH
LWETTMNPETRILKQVTIDNAANVDYIFSMLMGDDVAPRREFIEKNATYANIDA
HHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]