Click here to switch to the map view.

The map label for this gene is buk

Identifier: 150003887

GI number: 150003887

Start: 1769277

End: 1770341

Strand: Direct

Name: buk

Synonym: BVU_1320

Alternate gene names: 150003887

Gene position: 1769277-1770341 (Clockwise)

Preceding gene: 150003886

Following gene: 150003894

Centisome position: 34.27

GC content: 52.86

Gene sequence:

>1065_bases
ATGAAAATATTTGTGATTAATCCGGGCTCTACTTCTACCAAAATAGCCCTTTTCATCGATGAAAAGCCGGTGTGGGCGGC
AGGGGCCCATCATACTGCGGATGACTTGTCGGAGTTTCACCACGTCAACGAACAGTATGCGTACCGTAAGGACTTTGTAT
TGCGATTGCTTGCCGAGGCGGATATTCCGTTGGATTTTGATGCCGTCATTGCCCGTGGCGGTCTGCTGAAGCCTACGCCC
GGCGGGGTGTATGCCATTAACGAACAGATGAAACACGATTTGCTCAACGCCCGGATGGAGCACGCCTGTAATTTGGGCGC
GCTGATTGCCGATGAAATAGCCCGGGAATGCCATTGTCCCGCCTACATTGCCGATCCCGAAGTGGTAGACGAGCTGCAAC
CGGCCGCCCGCCTGACGGGAATACCGGAGATAGAGCGCATTTCCATCTTCCATGCCTTGAACAGTAAGGCGGTATCGCGT
AAATATGCCGCTTCCATCGGGAAACATTATGAAGAGTTGAATCTGATTGTGGTGCATCTGGGGGGTGGCATATCGGTGGG
TGCCCATTGCAAAGGACGGGTGATTGATGTTAACAATGCCTTGAACGGTGAGGGGCCGTTTTCTCCCGAACGTGCGGGAA
CCATTCCTGCCGACCAGTTAGCGGAATTGTGCTTCAGCGGTAAGTATACGTTAAAGCAGATCAAGAAGATGCTGAATGGC
AAAGGGGGGCTGACTGCCCATCTGGGTATGAATGATGTGGTGACTATTGCCCGGAAAGCTTCCGAAGGCGAGGAGCCTTA
TAAAGGGGTATTGGATGCGATGCTCTATACGGTGGCGAAACAGGCAGGGGCCATGTATGTCACGCTCCGTGGGCAGGTGG
ATGCCATTATTCTGACGGGAGGCATTGCCCATAGTGATTACTGTGTAGGGATTCTCAAGGAACAGATTGATTATCTGGCG
CCGGTTGTGCTGATGCCCGGTGAAGACGAGATGGGCTCGCTGGCTTATAATGCGTTGGGAGCCTTGAAAGGTGAGCTGCC
TTTGCAGGTTTACCGGCCGGAATAA

Upstream 100 bases:

>100_bases
AAATAATGTGGCAATGTGCCAATTTGCCGGTGTGTCAATTGGCTGTGCTGTGTTGCCGCATGGTTTTGGCTCATTCGCAA
ATTGATAAATTATTGAAACT

Downstream 100 bases:

>100_bases
GAAGATGGTGGTACATCGGAATTAAAATAAGCGATAAAATTGTTCAAATCCTTCATGGAAGGGTAATGTGTTGATATACA
GAAGGCTATTATGAAGGAAT

Product: butyrate kinase

Products: NA

Alternate protein names: BK; Branched-chain carboxylic acid kinase

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKIFVINPGSTSTKIALFIDEKPVWAAGAHHTADDLSEFHHVNEQYAYRKDFVLRLLAEADIPLDFDAVIARGGLLKPTP
GGVYAINEQMKHDLLNARMEHACNLGALIADEIARECHCPAYIADPEVVDELQPAARLTGIPEIERISIFHALNSKAVSR
KYAASIGKHYEELNLIVVHLGGGISVGAHCKGRVIDVNNALNGEGPFSPERAGTIPADQLAELCFSGKYTLKQIKKMLNG
KGGLTAHLGMNDVVTIARKASEGEEPYKGVLDAMLYTVAKQAGAMYVTLRGQVDAIILTGGIAHSDYCVGILKEQIDYLA
PVVLMPGEDEMGSLAYNALGALKGELPLQVYRPE

Sequences:

>Translated_354_residues
MKIFVINPGSTSTKIALFIDEKPVWAAGAHHTADDLSEFHHVNEQYAYRKDFVLRLLAEADIPLDFDAVIARGGLLKPTP
GGVYAINEQMKHDLLNARMEHACNLGALIADEIARECHCPAYIADPEVVDELQPAARLTGIPEIERISIFHALNSKAVSR
KYAASIGKHYEELNLIVVHLGGGISVGAHCKGRVIDVNNALNGEGPFSPERAGTIPADQLAELCFSGKYTLKQIKKMLNG
KGGLTAHLGMNDVVTIARKASEGEEPYKGVLDAMLYTVAKQAGAMYVTLRGQVDAIILTGGIAHSDYCVGILKEQIDYLA
PVVLMPGEDEMGSLAYNALGALKGELPLQVYRPE
>Mature_354_residues
MKIFVINPGSTSTKIALFIDEKPVWAAGAHHTADDLSEFHHVNEQYAYRKDFVLRLLAEADIPLDFDAVIARGGLLKPTP
GGVYAINEQMKHDLLNARMEHACNLGALIADEIARECHCPAYIADPEVVDELQPAARLTGIPEIERISIFHALNSKAVSR
KYAASIGKHYEELNLIVVHLGGGISVGAHCKGRVIDVNNALNGEGPFSPERAGTIPADQLAELCFSGKYTLKQIKKMLNG
KGGLTAHLGMNDVVTIARKASEGEEPYKGVLDAMLYTVAKQAGAMYVTLRGQVDAIILTGGIAHSDYCVGILKEQIDYLA
PVVLMPGEDEMGSLAYNALGALKGELPLQVYRPE

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=180, Percent_Identity=28.8888888888889, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): BUK_BACV8 (A6KZZ5)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298631.1
- ProteinModelPortal:   A6KZZ5
- SMR:   A6KZZ5
- STRING:   A6KZZ5
- GeneID:   5302286
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1320
- NMPDR:   fig|435590.6.peg.1245
- eggNOG:   COG3426
- HOGENOM:   HBG449785
- OMA:   RSIFHAL
- ProtClustDB:   PRK03011
- GO:   GO:0005737
- HAMAP:   MF_00542
- InterPro:   IPR000890
- InterPro:   IPR011245
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF3
- PIRSF:   PIRSF036458
- PRINTS:   PR00471
- TIGRFAMs:   TIGR02707

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.7

Molecular weight: Translated: 38267; Mature: 38267

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFVINPGSTSTKIALFIDEKPVWAAGAHHTADDLSEFHHVNEQYAYRKDFVLRLLAEA
CEEEEECCCCCCEEEEEEEECCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHC
DIPLDFDAVIARGGLLKPTPGGVYAINEQMKHDLLNARMEHACNLGALIADEIARECHCP
CCCCCHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
AYIADPEVVDELQPAARLTGIPEIERISIFHALNSKAVSRKYAASIGKHYEELNLIVVHL
CEECCHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEE
GGGISVGAHCKGRVIDVNNALNGEGPFSPERAGTIPADQLAELCFSGKYTLKQIKKMLNG
CCCEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCC
KGGLTAHLGMNDVVTIARKASEGEEPYKGVLDAMLYTVAKQAGAMYVTLRGQVDAIILTG
CCCEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCEEEEEEEC
GIAHSDYCVGILKEQIDYLAPVVLMPGEDEMGSLAYNALGALKGELPLQVYRPE
CCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCC
>Mature Secondary Structure
MKIFVINPGSTSTKIALFIDEKPVWAAGAHHTADDLSEFHHVNEQYAYRKDFVLRLLAEA
CEEEEECCCCCCEEEEEEEECCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHC
DIPLDFDAVIARGGLLKPTPGGVYAINEQMKHDLLNARMEHACNLGALIADEIARECHCP
CCCCCHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
AYIADPEVVDELQPAARLTGIPEIERISIFHALNSKAVSRKYAASIGKHYEELNLIVVHL
CEECCHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEE
GGGISVGAHCKGRVIDVNNALNGEGPFSPERAGTIPADQLAELCFSGKYTLKQIKKMLNG
CCCEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCC
KGGLTAHLGMNDVVTIARKASEGEEPYKGVLDAMLYTVAKQAGAMYVTLRGQVDAIILTG
CCCEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCEEEEEEEC
GIAHSDYCVGILKEQIDYLAPVVLMPGEDEMGSLAYNALGALKGELPLQVYRPE
CCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA