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The map label for this gene is dusB [H]

Identifier: 150003817

GI number: 150003817

Start: 1675674

End: 1676591

Strand: Direct

Name: dusB [H]

Synonym: BVU_1249

Alternate gene names: 150003817

Gene position: 1675674-1676591 (Clockwise)

Preceding gene: 150003814

Following gene: 150003818

Centisome position: 32.45

GC content: 51.31

Gene sequence:

>918_bases
ATGAAACTGTACGCTGCCCCTTTGCAAGGTTTTACGGAAGCCCCCTGGAGAAATCTTCATCAGGAAGTTTTTGGTGGGAT
AGATGCTTATTATACCCCCTTTGTGCGGATGGAGAAAGGAGAGTTCCGTAACAAGGATGTACGGGAGATCGCTTCTGAAA
ACAATACTGTGTCCCGGCTGATTCCCCAGCTTATCGCTTCCACTCCTGCCGAACTGGAACGGCTGGCCGGTTTGTTTATA
GAGAAAGGGTACAAGGAAGCGGATATCAATATGGGTTGTCCTTTTCCCCTGATGACAGGGAAACATAAGGGTTCGGGCAT
ATTGCCGTATCCGGCCGAGGTGGAAGCTTTATTGAAAGAGCTGGTGCACTATCCGGAACTGGGTTTCTCCGTCAAGATGA
GATTGGGATGGGAGTGTGCGGATGAATGGCGGCCGTTGTTGCCTTTGTTGAACGCTGCCCCCTTGCGGCGGATCGTTCTG
CATCCTCGTATCGGGAAGCAACAGTACAAGGGAGAGGTGGACTTGGCTGCGTTCACCGCTTTCTATGAGGAGTGCGCCCA
TCCGTTGGTGTATAACGGTGACTTGCTGACGGTGGAAGATATCCGGAAGGTGGACGAGGAGTTTCCCCGCCTGGAGGGGG
TCATGCTGGGACGCGGGTTGCTGGCCAATCCTGCTTTGGCTTGGGAATATGCAAACGGAACCGTTTTTTCTCCCGATGAG
CTGCATGAAAAGGTGAAGGCGCTTCACGCACGCCTTCTTCAATATTATGAAGCCCATTTACAGGGAGAGGCGCAATTGCT
GAGTAAAATGAAGCCTTATTGGGAATACCTGCTGCCCGATGCGGACCGGAAGATAAAAAAAGCGATAAAGAAGGCTACAA
CGATGGATAAGTATTTGACAGCGGTGAACCAGCTGTAA

Upstream 100 bases:

>100_bases
ATCGCGTACCTTCATCTTCAGTTCATAAATATACTTTTCCATAAGGCAAAGGTATAAGAAAAATGTGTTACTTTGCAGAT
AAACTTAAAAAAAGAAACAA

Downstream 100 bases:

>100_bases
TGATGGAAAAGGGGCTTCGGTACATGAAAACCGGAGTGTTTCTTCAGTTCATTCAGCCGGTCTAACAGCTTGTAAAATAG
AGAGGTATCCTGATAGGAGA

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKLYAAPLQGFTEAPWRNLHQEVFGGIDAYYTPFVRMEKGEFRNKDVREIASENNTVSRLIPQLIASTPAELERLAGLFI
EKGYKEADINMGCPFPLMTGKHKGSGILPYPAEVEALLKELVHYPELGFSVKMRLGWECADEWRPLLPLLNAAPLRRIVL
HPRIGKQQYKGEVDLAAFTAFYEECAHPLVYNGDLLTVEDIRKVDEEFPRLEGVMLGRGLLANPALAWEYANGTVFSPDE
LHEKVKALHARLLQYYEAHLQGEAQLLSKMKPYWEYLLPDADRKIKKAIKKATTMDKYLTAVNQL

Sequences:

>Translated_305_residues
MKLYAAPLQGFTEAPWRNLHQEVFGGIDAYYTPFVRMEKGEFRNKDVREIASENNTVSRLIPQLIASTPAELERLAGLFI
EKGYKEADINMGCPFPLMTGKHKGSGILPYPAEVEALLKELVHYPELGFSVKMRLGWECADEWRPLLPLLNAAPLRRIVL
HPRIGKQQYKGEVDLAAFTAFYEECAHPLVYNGDLLTVEDIRKVDEEFPRLEGVMLGRGLLANPALAWEYANGTVFSPDE
LHEKVKALHARLLQYYEAHLQGEAQLLSKMKPYWEYLLPDADRKIKKAIKKATTMDKYLTAVNQL
>Mature_305_residues
MKLYAAPLQGFTEAPWRNLHQEVFGGIDAYYTPFVRMEKGEFRNKDVREIASENNTVSRLIPQLIASTPAELERLAGLFI
EKGYKEADINMGCPFPLMTGKHKGSGILPYPAEVEALLKELVHYPELGFSVKMRLGWECADEWRPLLPLLNAAPLRRIVL
HPRIGKQQYKGEVDLAAFTAFYEECAHPLVYNGDLLTVEDIRKVDEEFPRLEGVMLGRGLLANPALAWEYANGTVFSPDE
LHEKVKALHARLLQYYEAHLQGEAQLLSKMKPYWEYLLPDADRKIKKAIKKATTMDKYLTAVNQL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789660, Length=302, Percent_Identity=24.5033112582781, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788462, Length=296, Percent_Identity=26.0135135135135, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=29.0748898678414, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585320, Length=184, Percent_Identity=29.3478260869565, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34732; Mature: 34732

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYAAPLQGFTEAPWRNLHQEVFGGIDAYYTPFVRMEKGEFRNKDVREIASENNTVSRL
CCEECCCCCCCCCCHHHHHHHHHHCCHHHHHCCCEEECCCCCCCHHHHHHHHCCCHHHHH
IPQLIASTPAELERLAGLFIEKGYKEADINMGCPFPLMTGKHKGSGILPYPAEVEALLKE
HHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LVHYPELGFSVKMRLGWECADEWRPLLPLLNAAPLRRIVLHPRIGKQQYKGEVDLAAFTA
HHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHCCCHHHHHHHH
FYEECAHPLVYNGDLLTVEDIRKVDEEFPRLEGVMLGRGLLANPALAWEYANGTVFSPDE
HHHHHCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEEECCCCCEECHHH
LHEKVKALHARLLQYYEAHLQGEAQLLSKMKPYWEYLLPDADRKIKKAIKKATTMDKYLT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AVNQL
HHHCC
>Mature Secondary Structure
MKLYAAPLQGFTEAPWRNLHQEVFGGIDAYYTPFVRMEKGEFRNKDVREIASENNTVSRL
CCEECCCCCCCCCCHHHHHHHHHHCCHHHHHCCCEEECCCCCCCHHHHHHHHCCCHHHHH
IPQLIASTPAELERLAGLFIEKGYKEADINMGCPFPLMTGKHKGSGILPYPAEVEALLKE
HHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LVHYPELGFSVKMRLGWECADEWRPLLPLLNAAPLRRIVLHPRIGKQQYKGEVDLAAFTA
HHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHCCCHHHHHHHH
FYEECAHPLVYNGDLLTVEDIRKVDEEFPRLEGVMLGRGLLANPALAWEYANGTVFSPDE
HHHHHCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEEECCCCCEECHHH
LHEKVKALHARLLQYYEAHLQGEAQLLSKMKPYWEYLLPDADRKIKKAIKKATTMDKYLT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AVNQL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]