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The map label for this gene is trmH [C]

Identifier: 150003712

GI number: 150003712

Start: 1538739

End: 1539275

Strand: Reverse

Name: trmH [C]

Synonym: BVU_1143

Alternate gene names: 150003712

Gene position: 1539275-1538739 (Counterclockwise)

Preceding gene: 150003715

Following gene: 150003710

Centisome position: 29.81

GC content: 41.71

Gene sequence:

>537_bases
ATGAGGAAACTCAAAATAACAGAACTGAACCGGCTTACTGTAGACGAATTCAAGAAAGCCGATAAGTTGCCTTTAGCAGT
GGTGCTGGATGAAGTACGCAGTTTACACAACATAGGGTCAGTGTTTCGCACATCGGATGCATTTTTGGTAGATTGCATTT
ATTTATGTGGTATTACAGCCACTCCTCCCCATCCCGAAATGCATAAAACAGCTTTAGGAGCTGAGTATTCGGTGGAGTGG
AGATATAAAAAGAATACGCTTGATGCCGTTCAGGAACTTCATGATCAAGGGTTTACCGTATTGGCTATCGAACAGGTGGA
AGGCAGTACTTTGCTGGATAAACTGGAACTGGATGCCGATAAAAAATATGCCATCGTAATGGGAAATGAAGTGAAAGGGG
TACAGCAGGAAGTGGTCAATGCTTGCGACGGTTGTGTTGAGATTCCCCAATACGGCACAAAACATTCGTTGAATGTATCG
GTTACCACAGGAATTATTTTATGGGAGTTTGCCAATAAATTAATGAAATTCAGATAA

Upstream 100 bases:

>100_bases
AAAAGAGAGTAGGAGAGACTTCTTTTTCATTATATAGGCGTAAAGATCATAGATTTTTATGTAAGTTTGTAACCGTTAAT
AATAAAGTAGAAAATAGAGT

Downstream 100 bases:

>100_bases
TAAGAGTAAAATTCAAACAGTAGGAATCAGTTAGATTTGTCCGTTTTCAACTAACAATACCACCCGTTCGGTCAGTCGAT
CCTCTCCCTCCGGATGATAT

Product: tRNA/rRNA methyltransferase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MRKLKITELNRLTVDEFKKADKLPLAVVLDEVRSLHNIGSVFRTSDAFLVDCIYLCGITATPPHPEMHKTALGAEYSVEW
RYKKNTLDAVQELHDQGFTVLAIEQVEGSTLLDKLELDADKKYAIVMGNEVKGVQQEVVNACDGCVEIPQYGTKHSLNVS
VTTGIILWEFANKLMKFR

Sequences:

>Translated_178_residues
MRKLKITELNRLTVDEFKKADKLPLAVVLDEVRSLHNIGSVFRTSDAFLVDCIYLCGITATPPHPEMHKTALGAEYSVEW
RYKKNTLDAVQELHDQGFTVLAIEQVEGSTLLDKLELDADKKYAIVMGNEVKGVQQEVVNACDGCVEIPQYGTKHSLNVS
VTTGIILWEFANKLMKFR
>Mature_178_residues
MRKLKITELNRLTVDEFKKADKLPLAVVLDEVRSLHNIGSVFRTSDAFLVDCIYLCGITATPPHPEMHKTALGAEYSVEW
RYKKNTLDAVQELHDQGFTVLAIEQVEGSTLLDKLELDADKKYAIVMGNEVKGVQQEVVNACDGCVEIPQYGTKHSLNVS
VTTGIILWEFANKLMKFR

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI110825988, Length=150, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1790083, Length=148, Percent_Identity=32.4324324324324, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1790623, Length=146, Percent_Identity=29.4520547945205, Blast_Score=63, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204953, Length=154, Percent_Identity=31.8181818181818, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 20011; Mature: 20011

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLKITELNRLTVDEFKKADKLPLAVVLDEVRSLHNIGSVFRTSDAFLVDCIYLCGITA
CCCCEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
TPPHPEMHKTALGAEYSVEWRYKKNTLDAVQELHDQGFTVLAIEQVEGSTLLDKLELDAD
CCCCCCHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCC
KKYAIVMGNEVKGVQQEVVNACDGCVEIPQYGTKHSLNVSVTTGIILWEFANKLMKFR
CCEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKLKITELNRLTVDEFKKADKLPLAVVLDEVRSLHNIGSVFRTSDAFLVDCIYLCGITA
CCCCEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
TPPHPEMHKTALGAEYSVEWRYKKNTLDAVQELHDQGFTVLAIEQVEGSTLLDKLELDAD
CCCCCCHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCC
KKYAIVMGNEVKGVQQEVVNACDGCVEIPQYGTKHSLNVSVTTGIILWEFANKLMKFR
CCEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCEEEEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]