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The map label for this gene is purD [H]

Identifier: 150003664

GI number: 150003664

Start: 1457922

End: 1459190

Strand: Direct

Name: purD [H]

Synonym: BVU_1095

Alternate gene names: 150003664

Gene position: 1457922-1459190 (Clockwise)

Preceding gene: 150003663

Following gene: 150003665

Centisome position: 28.24

GC content: 47.12

Gene sequence:

>1269_bases
ATGAAACTGTTGCTATTAGGCTCAGGCGGACGTGAACACGCCCTGGCATGGAAGATTGCTCAAAGTCCCAAAATCGAGAA
ACTCTATATCGCTCCGGGAAATGCCGGCACAAGTAATGTAGGCGAGAATGTAGCGATTAAAGCAGATGACTTTACAGCAA
TCAGAGAGTTTGTAGTAAAGAATAATATAGATATGGTAGTGGTAGGTCCCGAAGACCCGTTAGTCAAAGGAATCTATGAC
TATTTCAAAAAAGATGAAGCGCTGAAAAACATTCCTGTTATCGGGCCTTCAAAAGCGGGTGCGGTACTGGAAGGCAGCAA
GGAGTTCGCCAAAGGCTTCATGCAACGCCATAATATCCCGACAGCCGGTTACAAAAGTATCACTGCCGCAAATCTGGAAG
AAGGATTAGCCTTTCTGGAAACATTGGAAGCTCCATACGTACTGAAAGCCGACGGTCTTTGCGCCGGAAAAGGCGTACTG
ATTCTGCCCACCCTGGAAGAAGCCAAGAAAGAATTGAAAGAAATGCTGAGCGGAATGTTCGGTGACGCTTCCGCCACCGT
AGTGATTGAAGAATTCCTGAGCGGTATAGAATGTTCCGTATTCGTTCTGACCGATGGAAAGAACTACAAGATTCTGCCCG
AAGCAAAAGACTACAAACGCATCGGCGAAGGCGACAAAGGGCTGAACACCGGTGGTATGGGTAGTGTATCTCCTGTTCCG
TTTGCCGACAAAGCATGGATGGAAAAAGTGGAAGAACGTATCATCCGCCCCACAGTAGAAGGACTGGCAGCAGAAGGCAT
CGACTATAAAGGCTTTATCTTCTTCGGTCTGATTAATGTAAAAGGCGAACCGATGGTCATTGAATACAATGTACGCATGG
GCGACCCGGAAACGGAAAGTGTCATGCTGCGCATCAAGAGTGACTTGGTGGAACTGCTTGAAGGAGTGGCAACAGGCAAT
CTGAATGAAAAAGTCCTGGAGATAGACCCCCGTTCGGCAGTATGCGTCATGCTGGTATCCGGCGGATATCCGGAACATTA
TGACAAGGGATTTGCCATCAGCGGAGTAGAAGAAATTGAAAACAGCATTGTATTCCATGCAGGAACAGCACTGAAAGACG
GACAGGTAGTCACTAGCGGCGGACGTGTCATTGCAGTCAGCTCCTACGGTGCCAATAAAGAAGAAGCCTTGGCACAATCA
TTCGCCAATGCGAAGAAAATCAATTTCGACAAGAAATATTTCCGTTCAGATATTGGATTCGACCTGTAA

Upstream 100 bases:

>100_bases
GGGCGACGGTCATAATATGTTCGGACGCGACCGTGTGCACTTGTATGAACGCATTACACGCTATTTTGAAGACCACTTAA
AATAAACAAAAGATAAGATT

Downstream 100 bases:

>100_bases
TCAGCGGACTATCACCCCATGGCAGGCTATGACAAATTCCAAATAGATATGGCAAAGGGAAGATTCACCTTCCCTGTTGC
CATCCTTATATGCCTGCTTC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKLLLLGSGGREHALAWKIAQSPKIEKLYIAPGNAGTSNVGENVAIKADDFTAIREFVVKNNIDMVVVGPEDPLVKGIYD
YFKKDEALKNIPVIGPSKAGAVLEGSKEFAKGFMQRHNIPTAGYKSITAANLEEGLAFLETLEAPYVLKADGLCAGKGVL
ILPTLEEAKKELKEMLSGMFGDASATVVIEEFLSGIECSVFVLTDGKNYKILPEAKDYKRIGEGDKGLNTGGMGSVSPVP
FADKAWMEKVEERIIRPTVEGLAAEGIDYKGFIFFGLINVKGEPMVIEYNVRMGDPETESVMLRIKSDLVELLEGVATGN
LNEKVLEIDPRSAVCVMLVSGGYPEHYDKGFAISGVEEIENSIVFHAGTALKDGQVVTSGGRVIAVSSYGANKEEALAQS
FANAKKINFDKKYFRSDIGFDL

Sequences:

>Translated_422_residues
MKLLLLGSGGREHALAWKIAQSPKIEKLYIAPGNAGTSNVGENVAIKADDFTAIREFVVKNNIDMVVVGPEDPLVKGIYD
YFKKDEALKNIPVIGPSKAGAVLEGSKEFAKGFMQRHNIPTAGYKSITAANLEEGLAFLETLEAPYVLKADGLCAGKGVL
ILPTLEEAKKELKEMLSGMFGDASATVVIEEFLSGIECSVFVLTDGKNYKILPEAKDYKRIGEGDKGLNTGGMGSVSPVP
FADKAWMEKVEERIIRPTVEGLAAEGIDYKGFIFFGLINVKGEPMVIEYNVRMGDPETESVMLRIKSDLVELLEGVATGN
LNEKVLEIDPRSAVCVMLVSGGYPEHYDKGFAISGVEEIENSIVFHAGTALKDGQVVTSGGRVIAVSSYGANKEEALAQS
FANAKKINFDKKYFRSDIGFDL
>Mature_422_residues
MKLLLLGSGGREHALAWKIAQSPKIEKLYIAPGNAGTSNVGENVAIKADDFTAIREFVVKNNIDMVVVGPEDPLVKGIYD
YFKKDEALKNIPVIGPSKAGAVLEGSKEFAKGFMQRHNIPTAGYKSITAANLEEGLAFLETLEAPYVLKADGLCAGKGVL
ILPTLEEAKKELKEMLSGMFGDASATVVIEEFLSGIECSVFVLTDGKNYKILPEAKDYKRIGEGDKGLNTGGMGSVSPVP
FADKAWMEKVEERIIRPTVEGLAAEGIDYKGFIFFGLINVKGEPMVIEYNVRMGDPETESVMLRIKSDLVELLEGVATGN
LNEKVLEIDPRSAVCVMLVSGGYPEHYDKGFAISGVEEIENSIVFHAGTALKDGQVVTSGGRVIAVSSYGANKEEALAQS
FANAKKINFDKKYFRSDIGFDL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=42.018779342723, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=42.018779342723, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=42.018779342723, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=42.018779342723, Blast_Score=322, Evalue=5e-88,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=48.0093676814988, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=40.4255319148936, Blast_Score=308, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6321203, Length=442, Percent_Identity=40.0452488687783, Blast_Score=311, Evalue=1e-85,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=40.2777777777778, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=40.2777777777778, Blast_Score=285, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45593; Mature: 45593

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLLLGSGGREHALAWKIAQSPKIEKLYIAPGNAGTSNVGENVAIKADDFTAIREFVVK
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHH
NNIDMVVVGPEDPLVKGIYDYFKKDEALKNIPVIGPSKAGAVLEGSKEFAKGFMQRHNIP
CCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHCCCC
TAGYKSITAANLEEGLAFLETLEAPYVLKADGLCAGKGVLILPTLEEAKKELKEMLSGMF
CCCCCHHHHHCHHHHHHHHHHHCCCEEEEECCEECCCCEEEEECHHHHHHHHHHHHHHHC
GDASATVVIEEFLSGIECSVFVLTDGKNYKILPEAKDYKRIGEGDKGLNTGGMGSVSPVP
CCCHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCC
FADKAWMEKVEERIIRPTVEGLAAEGIDYKGFIFFGLINVKGEPMVIEYNVRMGDPETES
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCHH
VMLRIKSDLVELLEGVATGNLNEKVLEIDPRSAVCVMLVSGGYPEHYDKGFAISGVEEIE
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEECCCCCHHCCCCEECCHHHHC
NSIVFHAGTALKDGQVVTSGGRVIAVSSYGANKEEALAQSFANAKKINFDKKYFRSDIGF
CCEEEEECCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHCHHCCCCCHHHHHHHCCC
DL
CC
>Mature Secondary Structure
MKLLLLGSGGREHALAWKIAQSPKIEKLYIAPGNAGTSNVGENVAIKADDFTAIREFVVK
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHH
NNIDMVVVGPEDPLVKGIYDYFKKDEALKNIPVIGPSKAGAVLEGSKEFAKGFMQRHNIP
CCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHCCCC
TAGYKSITAANLEEGLAFLETLEAPYVLKADGLCAGKGVLILPTLEEAKKELKEMLSGMF
CCCCCHHHHHCHHHHHHHHHHHCCCEEEEECCEECCCCEEEEECHHHHHHHHHHHHHHHC
GDASATVVIEEFLSGIECSVFVLTDGKNYKILPEAKDYKRIGEGDKGLNTGGMGSVSPVP
CCCHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCC
FADKAWMEKVEERIIRPTVEGLAAEGIDYKGFIFFGLINVKGEPMVIEYNVRMGDPETES
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCHH
VMLRIKSDLVELLEGVATGNLNEKVLEIDPRSAVCVMLVSGGYPEHYDKGFAISGVEEIE
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEECCCCCHHCCCCEECCHHHHC
NSIVFHAGTALKDGQVVTSGGRVIAVSSYGANKEEALAQSFANAKKINFDKKYFRSDIGF
CCEEEEECCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHCHHCCCCCHHHHHHHCCC
DL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]