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The map label for this gene is prtC [H]

Identifier: 150003653

GI number: 150003653

Start: 1443445

End: 1444707

Strand: Reverse

Name: prtC [H]

Synonym: BVU_1084

Alternate gene names: 150003653

Gene position: 1444707-1443445 (Counterclockwise)

Preceding gene: 150003655

Following gene: 150003650

Centisome position: 27.98

GC content: 44.81

Gene sequence:

>1263_bases
ATGAAAGCTTCTATAAAAGACTTTGAAATAATGGCTCCTGTAGGTTCACGTGAGTCGCTGGCGGCAGCCATACAGGCAGG
TGCGGATTCTATTTACTTCGGAATTGAGAGTCTGAATATGCGTGCACGTTCTGCCAGTACATTTACTGTCAATGATTTAC
GCGAGATAGCACAGATCTGTGATAAACATGGCATCAAGAGCTATCTTACGATCAATACCATTATTTATGATGAAGATATT
GCTTTGATGCGTACCATTGTGGATGCGGCTAAGGAGGCAGGCATCAGTGCCGTGATTGCTGCGGATGTGGCGGTAATGGC
TTATTGTTGCGAGGTGGGGCAGGAAGTACACCTTTCCACCCAATTAAATATCAGTAATGCCGGAGCGTTGAAATTTTATG
CTCGCTTTGCCGATGTAGTGGTATTGGCACGTGAATTGAATTTGAAGCAGGTGCGGGTGATTTATGATACTATCCAGAAG
GAACAAATTAAAGGTCCGAATGGAGAATTGGTCCGTATTGAGATGTTCTGCCACGGGGCACTCTGTATGGCAGTGTCGGG
CAAGTGTTATCTCAGTTTGCATGAGATGAATGCTTCTGCTAACCGGGGGGCTTGTATGCAGATATGTCGCCGTGCATACG
ATGTGAAGGATAAAGAGAGTGATATTGAACTGGAGGTGGACAATAAATACATCATGTCTCCTAAAGATTTGAAAACTATT
CATTTTATGGATGAGATGATAGAGGCTGGCGTGCGTGTCTTCAAGATCGAAGGGCGTGCCCGAGGACCTGAGTATGTACG
TACCGTGGTGGAATGTTACAAAGAAGCTATTTGTTCTTATCTGGAGGGCACTTTTACCGAGGAGAAGAAGCAGGCTTGGG
ATGAACGGCTGAAGACCGTATTCAATCGGGGATTCTGGAATGGGTATTATTTAGGACAGCGTTTGGGTGAATGGAGTAAG
AACTATGGTTCGGAGGCTACTGAGCGTAAAGTGTATGTGGGTAAGGGAATCAAGTACTTTTCTAATATTGGCGTTGCAGA
GTTTCTGGTGGAAGCTGCTGAGATGAAAGTAGGGGATAAGCTTCTGATCACAGGACCTACTACAGGAGCGGTATTTCTGA
CGTTGGATGAGGCGCGTGTGGATTTGAAACCGGTGGATGTGGTAAGAAAAGGTCAACATGTATCCTTTAAAGTACCCGAT
AAGGTACGTCCTAGTGACAAGCTTTATAAGTTGGTTTCTCCTGAGGATTTGAAGAAGAAATAA

Upstream 100 bases:

>100_bases
TAATTTATAATCTGTGTGAATCTGTGTAATCTGTGGTGAAATCAGGCTGCAAAAATACGGAAAAAAGCCTACCTTTGCAC
ATATATTAATCAAAAGAATA

Downstream 100 bases:

>100_bases
AACAGAAAGAATGTGTTTCTAAAGAGATATTGAAATTTAAATCTCTTATGATTAAAATCCGAAGAGAAGCTCCCTTTTCT
TATTTTATGTAGCAATGAAT

Product: collagenase precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKASIKDFEIMAPVGSRESLAAAIQAGADSIYFGIESLNMRARSASTFTVNDLREIAQICDKHGIKSYLTINTIIYDEDI
ALMRTIVDAAKEAGISAVIAADVAVMAYCCEVGQEVHLSTQLNISNAGALKFYARFADVVVLARELNLKQVRVIYDTIQK
EQIKGPNGELVRIEMFCHGALCMAVSGKCYLSLHEMNASANRGACMQICRRAYDVKDKESDIELEVDNKYIMSPKDLKTI
HFMDEMIEAGVRVFKIEGRARGPEYVRTVVECYKEAICSYLEGTFTEEKKQAWDERLKTVFNRGFWNGYYLGQRLGEWSK
NYGSEATERKVYVGKGIKYFSNIGVAEFLVEAAEMKVGDKLLITGPTTGAVFLTLDEARVDLKPVDVVRKGQHVSFKVPD
KVRPSDKLYKLVSPEDLKKK

Sequences:

>Translated_420_residues
MKASIKDFEIMAPVGSRESLAAAIQAGADSIYFGIESLNMRARSASTFTVNDLREIAQICDKHGIKSYLTINTIIYDEDI
ALMRTIVDAAKEAGISAVIAADVAVMAYCCEVGQEVHLSTQLNISNAGALKFYARFADVVVLARELNLKQVRVIYDTIQK
EQIKGPNGELVRIEMFCHGALCMAVSGKCYLSLHEMNASANRGACMQICRRAYDVKDKESDIELEVDNKYIMSPKDLKTI
HFMDEMIEAGVRVFKIEGRARGPEYVRTVVECYKEAICSYLEGTFTEEKKQAWDERLKTVFNRGFWNGYYLGQRLGEWSK
NYGSEATERKVYVGKGIKYFSNIGVAEFLVEAAEMKVGDKLLITGPTTGAVFLTLDEARVDLKPVDVVRKGQHVSFKVPD
KVRPSDKLYKLVSPEDLKKK
>Mature_420_residues
MKASIKDFEIMAPVGSRESLAAAIQAGADSIYFGIESLNMRARSASTFTVNDLREIAQICDKHGIKSYLTINTIIYDEDI
ALMRTIVDAAKEAGISAVIAADVAVMAYCCEVGQEVHLSTQLNISNAGALKFYARFADVVVLARELNLKQVRVIYDTIQK
EQIKGPNGELVRIEMFCHGALCMAVSGKCYLSLHEMNASANRGACMQICRRAYDVKDKESDIELEVDNKYIMSPKDLKTI
HFMDEMIEAGVRVFKIEGRARGPEYVRTVVECYKEAICSYLEGTFTEEKKQAWDERLKTVFNRGFWNGYYLGQRLGEWSK
NYGSEATERKVYVGKGIKYFSNIGVAEFLVEAAEMKVGDKLLITGPTTGAVFLTLDEARVDLKPVDVVRKGQHVSFKVPD
KVRPSDKLYKLVSPEDLKKK

Specific function: Expresses collagenase activity. Hydrolyzes type I collagen. May play a role in virulence [H]

COG id: COG0826

COG function: function code O; Collagenase and related proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U32 family [H]

Homologues:

Organism=Escherichia coli, GI87081910, Length=286, Percent_Identity=32.1678321678322, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789548, Length=328, Percent_Identity=28.9634146341463, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788397, Length=418, Percent_Identity=24.8803827751196, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001539 [H]

Pfam domain/function: PF01136 Peptidase_U32 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 47013; Mature: 47013

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS01276 PEPTIDASE_U32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASIKDFEIMAPVGSRESLAAAIQAGADSIYFGIESLNMRARSASTFTVNDLREIAQIC
CCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCEEEHHHHHHHHHHH
DKHGIKSYLTINTIIYDEDIALMRTIVDAAKEAGISAVIAADVAVMAYCCEVGQEVHLST
HHCCCCEEEEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEE
QLNISNAGALKFYARFADVVVLARELNLKQVRVIYDTIQKEQIKGPNGELVRIEMFCHGA
EEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCE
LCMAVSGKCYLSLHEMNASANRGACMQICRRAYDVKDKESDIELEVDNKYIMSPKDLKTI
EEEEECCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCEEECCCCHHHHH
HFMDEMIEAGVRVFKIEGRARGPEYVRTVVECYKEAICSYLEGTFTEEKKQAWDERLKTV
HHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FNRGFWNGYYLGQRLGEWSKNYGSEATERKVYVGKGIKYFSNIGVAEFLVEAAEMKVGDK
HHCCCCCHHHHHHHHHHHHHHCCCCHHHCEEEECCCHHHHHCCCHHHHHHHHHHHCCCCE
LLITGPTTGAVFLTLDEARVDLKPVDVVRKGQHVSFKVPDKVRPSDKLYKLVSPEDLKKK
EEEECCCCCEEEEEEECCCCCCCHHHHHHCCCEEEEECCCCCCCHHHHEECCCCHHHCCC
>Mature Secondary Structure
MKASIKDFEIMAPVGSRESLAAAIQAGADSIYFGIESLNMRARSASTFTVNDLREIAQIC
CCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCEEEHHHHHHHHHHH
DKHGIKSYLTINTIIYDEDIALMRTIVDAAKEAGISAVIAADVAVMAYCCEVGQEVHLST
HHCCCCEEEEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEE
QLNISNAGALKFYARFADVVVLARELNLKQVRVIYDTIQKEQIKGPNGELVRIEMFCHGA
EEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCE
LCMAVSGKCYLSLHEMNASANRGACMQICRRAYDVKDKESDIELEVDNKYIMSPKDLKTI
EEEEECCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCEEECCCCHHHHH
HFMDEMIEAGVRVFKIEGRARGPEYVRTVVECYKEAICSYLEGTFTEEKKQAWDERLKTV
HHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FNRGFWNGYYLGQRLGEWSKNYGSEATERKVYVGKGIKYFSNIGVAEFLVEAAEMKVGDK
HHCCCCCHHHHHHHHHHHHHHCCCCHHHCEEEECCCHHHHHCCCHHHHHHHHHHHCCCCE
LLITGPTTGAVFLTLDEARVDLKPVDVVRKGQHVSFKVPDKVRPSDKLYKLVSPEDLKKK
EEEECCCCCEEEEEEECCCCCCCHHHHHHCCCEEEEECCCCCCCHHHHEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]