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The map label for this gene is purN [H]

Identifier: 150003621

GI number: 150003621

Start: 1400194

End: 1400772

Strand: Direct

Name: purN [H]

Synonym: BVU_1052

Alternate gene names: 150003621

Gene position: 1400194-1400772 (Clockwise)

Preceding gene: 150003617

Following gene: 150003623

Centisome position: 27.12

GC content: 43.35

Gene sequence:

>579_bases
ATGAAGAAAATCGCAATTTTAGCTTCAGGAGAAGGCACGAATGCTGAAAGAATTATTCGGTATTTTTTAGAGAAAAGAAC
AGCTGAAGTAGCATTGGTTATCGTCAACAAAGCACAGGCCGGTGTACTCAAGCGAGCTGAAAGACTGAGTGTTCCGTCAC
TTATCCTCACAGCCCAAGACTTTGCCGACGGGAAAGCACTGGAAATACTGCACCAATATCATATTGACTTTATTGTACTG
GCAGGCTTCCTCTTGAAAGTACCTGATGCCATTTTGCATGATTATCCGAATAAAATAGTAAATATTCATCCGGCCCTCCT
TCCCAAGTTCGGTGGTAAAGGTATGTACGGTTCCCGCGTGCACCAAGCTGTCATAGCTTCCCATGAAAAGGAAAGCGGCA
TCACCATCCATTATATAAATGAACGATATGATGAAGGAAATACCATTTTCCAAGCCACCTGTCCGGTATTGCCCACTGAC
ACGCCAGATACATTGGCAATACGGGTGCACCAGTTGGAATACGAATATTTCCCTCGGGTGATTGAAGCGACTATTCTAGG
CAAAAATCTTTCAATCTAA

Upstream 100 bases:

>100_bases
CAAAGGAATAAATATTTCTTGGGCTGAGCAAATATTCAGGCAAATAAATGCAGAATTTTGCACATTTTTTTGTTTTATGT
GCACCAAAATAGAGAACATC

Downstream 100 bases:

>100_bases
TACGAAAAAACTGAAGAGCTTTGCCTATCCGATAATTGACAGTCTGCACCGACACCTCCAGCTTGTCAGCAATTTCTTTA
TGGGTCAGTTGTTTATACCG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MKKIAILASGEGTNAERIIRYFLEKRTAEVALVIVNKAQAGVLKRAERLSVPSLILTAQDFADGKALEILHQYHIDFIVL
AGFLLKVPDAILHDYPNKIVNIHPALLPKFGGKGMYGSRVHQAVIASHEKESGITIHYINERYDEGNTIFQATCPVLPTD
TPDTLAIRVHQLEYEYFPRVIEATILGKNLSI

Sequences:

>Translated_192_residues
MKKIAILASGEGTNAERIIRYFLEKRTAEVALVIVNKAQAGVLKRAERLSVPSLILTAQDFADGKALEILHQYHIDFIVL
AGFLLKVPDAILHDYPNKIVNIHPALLPKFGGKGMYGSRVHQAVIASHEKESGITIHYINERYDEGNTIFQATCPVLPTD
TPDTLAIRVHQLEYEYFPRVIEATILGKNLSI
>Mature_192_residues
MKKIAILASGEGTNAERIIRYFLEKRTAEVALVIVNKAQAGVLKRAERLSVPSLILTAQDFADGKALEILHQYHIDFIVL
AGFLLKVPDAILHDYPNKIVNIHPALLPKFGGKGMYGSRVHQAVIASHEKESGITIHYINERYDEGNTIFQATCPVLPTD
TPDTLAIRVHQLEYEYFPRVIEATILGKNLSI

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=186, Percent_Identity=30.6451612903226, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI209869995, Length=186, Percent_Identity=30.6451612903226, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI209869993, Length=186, Percent_Identity=30.6451612903226, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788846, Length=192, Percent_Identity=32.2916666666667, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=185, Percent_Identity=30.2702702702703, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24582400, Length=197, Percent_Identity=36.0406091370558, Blast_Score=127, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21385; Mature: 21385

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAILASGEGTNAERIIRYFLEKRTAEVALVIVNKAQAGVLKRAERLSVPSLILTAQD
CCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEEECC
FADGKALEILHQYHIDFIVLAGFLLKVPDAILHDYPNKIVNIHPALLPKFGGKGMYGSRV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECHHHCCCCCCCCCCHHHH
HQAVIASHEKESGITIHYINERYDEGNTIFQATCPVLPTDTPDTLAIRVHQLEYEYFPRV
HHHHHHCCCCCCCCEEEEECCEECCCCEEEEEECCCCCCCCCCEEEEEEEEHHHHHHHHH
IEATILGKNLSI
HHHHHCCCCCCC
>Mature Secondary Structure
MKKIAILASGEGTNAERIIRYFLEKRTAEVALVIVNKAQAGVLKRAERLSVPSLILTAQD
CCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEEECC
FADGKALEILHQYHIDFIVLAGFLLKVPDAILHDYPNKIVNIHPALLPKFGGKGMYGSRV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECHHHCCCCCCCCCCHHHH
HQAVIASHEKESGITIHYINERYDEGNTIFQATCPVLPTDTPDTLAIRVHQLEYEYFPRV
HHHHHHCCCCCCCCEEEEECCEECCCCEEEEEECCCCCCCCCCEEEEEEEEHHHHHHHHH
IEATILGKNLSI
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]