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The map label for this gene is pfkA3 [H]

Identifier: 150003611

GI number: 150003611

Start: 1380224

End: 1381234

Strand: Direct

Name: pfkA3 [H]

Synonym: BVU_1040

Alternate gene names: 150003611

Gene position: 1380224-1381234 (Clockwise)

Preceding gene: 150003607

Following gene: 150003612

Centisome position: 26.73

GC content: 45.99

Gene sequence:

>1011_bases
ATGAAGATAGGAATATTGACATCAGGAGGAGACTGTCCAGGAATAAACGCCACAATTAGAGGAGTTTGCAAAACAGCGAT
AAATCATTACGGTATGGAAGTTTATGGAATTCATAGCGGATTCCGGGGATTGCTGGACAATGACGTAGAACCATTGACAG
AAAAATCATTATCGGGCTTGTTAAATCTGGGAGGGACAATACTAGGCACTTCCAGAGAAAAGCCATTCAAGAAACGCCTG
TCGGCAGCCTCGGAGGACAAACCGGCTTTGATGTTAAAGAATATCCATGATCTAGGTCTGGACTGTATCGTATGTATCGG
CGGAAACGGAACCCAAAAGACCGCAGCCAAACTGGCCCAAGCAGGTGTGAACGTTGTTTCTGTACCTAAAACAATAGATA
ACGATGTTTGGGGAACAGACGTTTCATTCGGATTCGATTCTGCCGTGACCATTGCTACCGATGCCATTGACCGGCTGCAT
TCTACAGCCAGTTCACACCAACGTGTCATGGTTATTGAAGTGATGGGACATAAAGCTGGCTGGATTGCACTTTATTCCGG
AATGGCGGGAGGCGGTGACGTGATTCTTATTCCGGAACTGTCTTATGATATTCATAATATAGGCAATACCATCATTGAAC
GCTTGAAAAAAGGGAAGCCCTATTCCATTGTTGTTGTCGCAGAAGGCATCAAAACCATGGACGGCAAAAAAGCCGCCGAA
TATATAGCCCAAGAAATAGAATATGAAACCGGATTCGAGACCCGTGAAACTGTACTGGGATATATCCAGCGTGGCGGCTC
TCCTACTCCCTACGACCGTAATCTGGCCACTCGTATGGGTGGACATGCCACAGAACTGATTGCTTCCGGACAATTCGGAC
GAATGGTCTCTCTGCAAGGTGCGGAAATTGGCTCCATCCCTCTGAATGAAGTGGCTGGTAAATTAAAACTTGTCAATGAG
AACCATGACCTGATTGTACAAGGCAAACGGATGGGAATCTGCTTCGGATAA

Upstream 100 bases:

>100_bases
TATATTATAATGGCTGATAACATTATTTTTTTTATAGTTTTCTACATTACTTTTATTTTTTGTTATCTTTACGGTCGCTT
ACAACTTAACATCAAAGGCT

Downstream 100 bases:

>100_bases
GAAGCAGATTCAATCCTTTTCCATATACTTGGATGGAGACACTCCGAATTGCTCGGTAAAATGCTTTCGGAGCGTACGTG
TATCACTATATCCTACCATT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 3; Phosphohexokinase 3 [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKIGILTSGGDCPGINATIRGVCKTAINHYGMEVYGIHSGFRGLLDNDVEPLTEKSLSGLLNLGGTILGTSREKPFKKRL
SAASEDKPALMLKNIHDLGLDCIVCIGGNGTQKTAAKLAQAGVNVVSVPKTIDNDVWGTDVSFGFDSAVTIATDAIDRLH
STASSHQRVMVIEVMGHKAGWIALYSGMAGGGDVILIPELSYDIHNIGNTIIERLKKGKPYSIVVVAEGIKTMDGKKAAE
YIAQEIEYETGFETRETVLGYIQRGGSPTPYDRNLATRMGGHATELIASGQFGRMVSLQGAEIGSIPLNEVAGKLKLVNE
NHDLIVQGKRMGICFG

Sequences:

>Translated_336_residues
MKIGILTSGGDCPGINATIRGVCKTAINHYGMEVYGIHSGFRGLLDNDVEPLTEKSLSGLLNLGGTILGTSREKPFKKRL
SAASEDKPALMLKNIHDLGLDCIVCIGGNGTQKTAAKLAQAGVNVVSVPKTIDNDVWGTDVSFGFDSAVTIATDAIDRLH
STASSHQRVMVIEVMGHKAGWIALYSGMAGGGDVILIPELSYDIHNIGNTIIERLKKGKPYSIVVVAEGIKTMDGKKAAE
YIAQEIEYETGFETRETVLGYIQRGGSPTPYDRNLATRMGGHATELIASGQFGRMVSLQGAEIGSIPLNEVAGKLKLVNE
NHDLIVQGKRMGICFG
>Mature_336_residues
MKIGILTSGGDCPGINATIRGVCKTAINHYGMEVYGIHSGFRGLLDNDVEPLTEKSLSGLLNLGGTILGTSREKPFKKRL
SAASEDKPALMLKNIHDLGLDCIVCIGGNGTQKTAAKLAQAGVNVVSVPKTIDNDVWGTDVSFGFDSAVTIATDAIDRLH
STASSHQRVMVIEVMGHKAGWIALYSGMAGGGDVILIPELSYDIHNIGNTIIERLKKGKPYSIVVVAEGIKTMDGKKAAE
YIAQEIEYETGFETRETVLGYIQRGGSPTPYDRNLATRMGGHATELIASGQFGRMVSLQGAEIGSIPLNEVAGKLKLVNE
NHDLIVQGKRMGICFG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=356, Percent_Identity=33.9887640449438, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI266453768, Length=356, Percent_Identity=33.9887640449438, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI266453748, Length=356, Percent_Identity=33.9887640449438, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4505749, Length=356, Percent_Identity=33.9887640449438, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI11321601, Length=356, Percent_Identity=33.4269662921348, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI48762920, Length=355, Percent_Identity=32.9577464788732, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1790350, Length=302, Percent_Identity=43.046357615894, Blast_Score=246, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI25147584, Length=355, Percent_Identity=31.2676056338028, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17558788, Length=362, Percent_Identity=29.2817679558011, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI72003601, Length=300, Percent_Identity=27.6666666666667, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321679, Length=316, Percent_Identity=31.9620253164557, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6323861, Length=317, Percent_Identity=32.1766561514196, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI17647809, Length=351, Percent_Identity=32.7635327635328, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24652337, Length=351, Percent_Identity=32.7635327635328, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28573326, Length=352, Percent_Identity=32.6704545454545, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35786; Mature: 35786

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGILTSGGDCPGINATIRGVCKTAINHYGMEVYGIHSGFRGLLDNDVEPLTEKSLSGL
CEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCHHHHHHHHHH
LNLGGTILGTSREKPFKKRLSAASEDKPALMLKNIHDLGLDCIVCIGGNGTQKTAAKLAQ
HHCCCEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
AGVNVVSVPKTIDNDVWGTDVSFGFDSAVTIATDAIDRLHSTASSHQRVMVIEVMGHKAG
CCCCEEECCCHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
WIALYSGMAGGGDVILIPELSYDIHNIGNTIIERLKKGKPYSIVVVAEGIKTMDGKKAAE
EEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHH
YIAQEIEYETGFETRETVLGYIQRGGSPTPYDRNLATRMGGHATELIASGQFGRMVSLQG
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHCCCCCCEEEECC
AEIGSIPLNEVAGKLKLVNENHDLIVQGKRMGICFG
CCCCCCCHHHHCCEEEEEECCCCEEEECCEEEEECC
>Mature Secondary Structure
MKIGILTSGGDCPGINATIRGVCKTAINHYGMEVYGIHSGFRGLLDNDVEPLTEKSLSGL
CEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCHHHHHHHHHH
LNLGGTILGTSREKPFKKRLSAASEDKPALMLKNIHDLGLDCIVCIGGNGTQKTAAKLAQ
HHCCCEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
AGVNVVSVPKTIDNDVWGTDVSFGFDSAVTIATDAIDRLHSTASSHQRVMVIEVMGHKAG
CCCCEEECCCHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
WIALYSGMAGGGDVILIPELSYDIHNIGNTIIERLKKGKPYSIVVVAEGIKTMDGKKAAE
EEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHH
YIAQEIEYETGFETRETVLGYIQRGGSPTPYDRNLATRMGGHATELIASGQFGRMVSLQG
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHCCCCCCEEEECC
AEIGSIPLNEVAGKLKLVNENHDLIVQGKRMGICFG
CCCCCCCHHHHCCEEEEEECCCCEEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]