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The map label for this gene is pckA [H]

Identifier: 150003549

GI number: 150003549

Start: 1289628

End: 1291235

Strand: Reverse

Name: pckA [H]

Synonym: BVU_0976

Alternate gene names: 150003549

Gene position: 1291235-1289628 (Counterclockwise)

Preceding gene: 150003551

Following gene: 150003548

Centisome position: 25.01

GC content: 41.92

Gene sequence:

>1608_bases
ATGGCAAATTTAGATTTAAGCAAGTACGGTATTACCGACGTGAAGGAAGTTCTCCACAATCCTTCTTATGATGTGCTTTT
CGCTGAAGAAACTAAACCGGGATTGGAAGGTTTCGAAAAAGGACAGGTTACTGAACTGGGTGCTGTAAACGTAATGACTG
GTGTTTACACAGGTCGTTCTCCTAAAGATAAATTCTTCGTTAAGAATGAAGCTTCTGAAAATTCAGTATGGTGGACTTCT
GACGAATATAAAAACGATAACAAACCTTGTACTGAAGAAGCTTGGGCTGACTTGAAGTCTAAAGCTGTAAAACAATTGAG
CGGCAAGCGTTTGTTCGTTGTTGATACATTCTGCGGTGCTAATGCTGCAACTCGTATGAAAGTACGTTTCATCATGGAAG
TAGCTTGGCAGGCTCATTTCGTAACTAACATGTTCATCCGCCCGACTGCTGAAGAACTGGAAGCTTACGGAGAACCTGAT
TTTGTATGTTTCAATGCATCTAAAGCAAAAGTTGATAACTATAAAGAACTGGGCTTGAACTCTGAAACAGCTACTGTTTT
CAACTTGAAGACTAAAGAACAAGTTATCCTGAACACTTGGTACGGTGGTGAAATGAAGAAAGGTATGTTCTCTATCATGA
ACTACATGAACCCGCTTCGTGGTATCGCTTCCATGCACTGCTCTGCTAATACAAATATGGAAGGAACTAGTTCGGCTATC
TTCTTCGGTTTGTCTGGTACAGGTAAGACTACTTTGTCTACTGACCCGAAACGTAAGTTGATCGGTGACGACGAACACGG
ATGGGATGACGAAGGTGTATTCAACTACGAAGGTGGTTGCTATGCTAAAGTTATCAACTTGGATAAGGAAAGTGAACCCG
ATATCTACGCTGCTATCAAACGTGACGCTTTGCTGGAAAACGTAACAGTTGATGCTAACGGTAAGATTGATTTCGCTGAT
AAGAGCGTAACAGAAAACACTCGTGTTTCTTATCCTATCTATCATATCGAAAACATCGTTAAACCTATCTCTAAAGGTCC
TCACGCTCAACAAGTTATTTTCTTGTCAGCTGATGCATTCGGTGTATTGCCTCCGGTATCTATCTTGAACCCTGAACAAG
CTCAGTATTACTTCTTGTCTGGATTTACAGCTAAATTGGCTGGTACAGAACGTGGTATTACTGAACCGACTCCGACATTC
TCTGCTTGCTTCGGTGCTGCTTTCTTGTCATTGCATCCTACTAAGTATGCTGAAGAATTGGTTAAGAAGATGGAAAAGAC
TGGTGCTAAAGCATATTTGGTTAACACTGGTTGGAACGGTACTGGTAAACGTATCTCTATCCGCGATACTCGTGGTATCA
TCGATGCTATTTTGGATGGCTCTATTGATAAGGCTCCTACTAAGGTTATTCCTTACTTCGATTTCGTTGTTCCTACAGAA
TTGCCGGGTGTTGATCCGAAGATCCTTGATCCTCGTGACACTTACGCTGACGCTGCTCAGTGGGATGAAAAAGCAAAAGA
CTTGGCTGGTCGTTTCATCAAGAACTTCGCTAAGTTCACTGGTAACGAAGCAGGTAAGAAATTGGTTGCTGCCGGTCCGA
AATTGTAA

Upstream 100 bases:

>100_bases
TTTGTAGCGCCTTAGACAAGGGGGTGTTGAAAAACATCTTCTTTCAGAAAATAAAGAGTAAAAATAACATTATACAATAA
CCAAATTAAATCATTAAAAA

Downstream 100 bases:

>100_bases
TCTGAATTTTACAGATTTAACATATAAAAAGTGGTTCCTATCGGGAACCACTTTTTTTTATGCTTGAAAATGCCTAGATT
TGTTGCAAACCAAATATGAT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MANLDLSKYGITDVKEVLHNPSYDVLFAEETKPGLEGFEKGQVTELGAVNVMTGVYTGRSPKDKFFVKNEASENSVWWTS
DEYKNDNKPCTEEAWADLKSKAVKQLSGKRLFVVDTFCGANAATRMKVRFIMEVAWQAHFVTNMFIRPTAEELEAYGEPD
FVCFNASKAKVDNYKELGLNSETATVFNLKTKEQVILNTWYGGEMKKGMFSIMNYMNPLRGIASMHCSANTNMEGTSSAI
FFGLSGTGKTTLSTDPKRKLIGDDEHGWDDEGVFNYEGGCYAKVINLDKESEPDIYAAIKRDALLENVTVDANGKIDFAD
KSVTENTRVSYPIYHIENIVKPISKGPHAQQVIFLSADAFGVLPPVSILNPEQAQYYFLSGFTAKLAGTERGITEPTPTF
SACFGAAFLSLHPTKYAEELVKKMEKTGAKAYLVNTGWNGTGKRISIRDTRGIIDAILDGSIDKAPTKVIPYFDFVVPTE
LPGVDPKILDPRDTYADAAQWDEKAKDLAGRFIKNFAKFTGNEAGKKLVAAGPKL

Sequences:

>Translated_535_residues
MANLDLSKYGITDVKEVLHNPSYDVLFAEETKPGLEGFEKGQVTELGAVNVMTGVYTGRSPKDKFFVKNEASENSVWWTS
DEYKNDNKPCTEEAWADLKSKAVKQLSGKRLFVVDTFCGANAATRMKVRFIMEVAWQAHFVTNMFIRPTAEELEAYGEPD
FVCFNASKAKVDNYKELGLNSETATVFNLKTKEQVILNTWYGGEMKKGMFSIMNYMNPLRGIASMHCSANTNMEGTSSAI
FFGLSGTGKTTLSTDPKRKLIGDDEHGWDDEGVFNYEGGCYAKVINLDKESEPDIYAAIKRDALLENVTVDANGKIDFAD
KSVTENTRVSYPIYHIENIVKPISKGPHAQQVIFLSADAFGVLPPVSILNPEQAQYYFLSGFTAKLAGTERGITEPTPTF
SACFGAAFLSLHPTKYAEELVKKMEKTGAKAYLVNTGWNGTGKRISIRDTRGIIDAILDGSIDKAPTKVIPYFDFVVPTE
LPGVDPKILDPRDTYADAAQWDEKAKDLAGRFIKNFAKFTGNEAGKKLVAAGPKL
>Mature_534_residues
ANLDLSKYGITDVKEVLHNPSYDVLFAEETKPGLEGFEKGQVTELGAVNVMTGVYTGRSPKDKFFVKNEASENSVWWTSD
EYKNDNKPCTEEAWADLKSKAVKQLSGKRLFVVDTFCGANAATRMKVRFIMEVAWQAHFVTNMFIRPTAEELEAYGEPDF
VCFNASKAKVDNYKELGLNSETATVFNLKTKEQVILNTWYGGEMKKGMFSIMNYMNPLRGIASMHCSANTNMEGTSSAIF
FGLSGTGKTTLSTDPKRKLIGDDEHGWDDEGVFNYEGGCYAKVINLDKESEPDIYAAIKRDALLENVTVDANGKIDFADK
SVTENTRVSYPIYHIENIVKPISKGPHAQQVIFLSADAFGVLPPVSILNPEQAQYYFLSGFTAKLAGTERGITEPTPTFS
ACFGAAFLSLHPTKYAEELVKKMEKTGAKAYLVNTGWNGTGKRISIRDTRGIIDAILDGSIDKAPTKVIPYFDFVVPTEL
PGVDPKILDPRDTYADAAQWDEKAKDLAGRFIKNFAKFTGNEAGKKLVAAGPKL

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=532, Percent_Identity=67.8571428571429, Blast_Score=754, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=505, Percent_Identity=44.950495049505, Blast_Score=448, Evalue=1e-126,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59089; Mature: 58958

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLDLSKYGITDVKEVLHNPSYDVLFAEETKPGLEGFEKGQVTELGAVNVMTGVYTGRS
CCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEECCCEEEEEEECCCCC
PKDKFFVKNEASENSVWWTSDEYKNDNKPCTEEAWADLKSKAVKQLSGKRLFVVDTFCGA
CCCCEEEECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
NAATRMKVRFIMEVAWQAHFVTNMFIRPTAEELEAYGEPDFVCFNASKAKVDNYKELGLN
CCHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHCCCCCEEEEECCCCCCCCHHHCCCC
SETATVFNLKTKEQVILNTWYGGEMKKGMFSIMNYMNPLRGIASMHCSANTNMEGTSSAI
CCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
FFGLSGTGKTTLSTDPKRKLIGDDEHGWDDEGVFNYEGGCYAKVINLDKESEPDIYAAIK
EEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCHHHHHH
RDALLENVTVDANGKIDFADKSVTENTRVSYPIYHIENIVKPISKGPHAQQVIFLSADAF
HHHHHHCEEECCCCEEEECCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCC
GVLPPVSILNPEQAQYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLSLHPTKYAEEL
CCCCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
VKKMEKTGAKAYLVNTGWNGTGKRISIRDTRGIIDAILDGSIDKAPTKVIPYFDFVVPTE
HHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCEEECEEEEEECCC
LPGVDPKILDPRDTYADAAQWDEKAKDLAGRFIKNFAKFTGNEAGKKLVAAGPKL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCEEECCCCCC
>Mature Secondary Structure 
ANLDLSKYGITDVKEVLHNPSYDVLFAEETKPGLEGFEKGQVTELGAVNVMTGVYTGRS
CCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEECCCEEEEEEECCCCC
PKDKFFVKNEASENSVWWTSDEYKNDNKPCTEEAWADLKSKAVKQLSGKRLFVVDTFCGA
CCCCEEEECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
NAATRMKVRFIMEVAWQAHFVTNMFIRPTAEELEAYGEPDFVCFNASKAKVDNYKELGLN
CCHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHCCCCCEEEEECCCCCCCCHHHCCCC
SETATVFNLKTKEQVILNTWYGGEMKKGMFSIMNYMNPLRGIASMHCSANTNMEGTSSAI
CCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
FFGLSGTGKTTLSTDPKRKLIGDDEHGWDDEGVFNYEGGCYAKVINLDKESEPDIYAAIK
EEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCHHHHHH
RDALLENVTVDANGKIDFADKSVTENTRVSYPIYHIENIVKPISKGPHAQQVIFLSADAF
HHHHHHCEEECCCCEEEECCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCC
GVLPPVSILNPEQAQYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLSLHPTKYAEEL
CCCCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
VKKMEKTGAKAYLVNTGWNGTGKRISIRDTRGIIDAILDGSIDKAPTKVIPYFDFVVPTE
HHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCEEECEEEEEECCC
LPGVDPKILDPRDTYADAAQWDEKAKDLAGRFIKNFAKFTGNEAGKKLVAAGPKL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA