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The map label for this gene is metG

Identifier: 150003455

GI number: 150003455

Start: 1185973

End: 1188015

Strand: Direct

Name: metG

Synonym: BVU_0882

Alternate gene names: 150003455

Gene position: 1185973-1188015 (Clockwise)

Preceding gene: 150003454

Following gene: 150003456

Centisome position: 22.97

GC content: 44.0

Gene sequence:

>2043_bases
ATGGAAAATAAATTCAAAAGAACCACCGTCACATCCGCTTTGCCCTACGCAAACGGTCCGGTACATATTGGTCATCTGGC
AGGTGTATATGTTCCTGCTGATATCTACGTGCGCTATCTCCGCCTGAAAAAAGAAGATGTTCTTTTCATCGGAGGCTCTG
ACGAACACGGAGTACCTATCACCATTCGTGCAAAGAAAGAAGGTATTACTCCACAAGATGTGGTGGACCGCTATCATACC
TTGATAAAAGAATCATTCAAAGAATTTGGAATTTCATTCGATATATACTCTCGTACTACATCAAAGACACACCACGAACT
GGCTTCCGAATTCTTCAAAACCCTGTATGATAAAGGTGAGTTCATTGAAAAGACCTCCATGCAATATTACGACGAAGAAG
CCCATCAGTTCCTGGCCGACCGTTATATTACCGGTGAATGCCCTCACTGCCATGCAGAAGGAGCCTACGGTGACCAATGC
GAGAAGTGCGGAACATCACTTTCTCCGACCGACCTGATCAATCCGAAAAGTGCTATCAGTGGCAGTAAACCAGTAATGCG
TGAAACCAAACATTGGTATCTGCCATTGGACAAACACGAAGGATGGCTGCGTGAATGGATTCTTGAAAATCATAAAGAAT
GGCGCCCCAATGTATACGGACAATGTAAAAGCTGGTTGGATATGGGCTTGCAACCCCGTGCGGTAAGCCGTGACTTGGAT
TGGGGAATCCCTGTTCCTGTAGAAGGCGCTGAGGGAAAAGTACTTTATGTATGGTTCGATGCCCCCATCGGCTATATTTC
AAATACAAAAGAATTACTTCCCGACAGTTGGGAAACTTGGTGGAAAGATCCAGAAACCCGTTTGATTCACTTCATCGGAA
AAGACAATATTGTATTCCACTGCATTGTTTTCCCTGCTATGCTGAAAGCGGAAGGAAGCTATATCTTACCGGATAACGTA
CCTAGCAATGAATTCCTGAACCTGGAAGGTGACAAGATTTCCACTTCACGCAACTGGGCAGTATGGTTGCATGAATATTT
GCAGGACTTCCCCGGCAAACAGGATGTATTGCGCTATGTATTGACAGCCAATGCCCCCGAAACAAAAGACAACGATTTTA
CCTGGAAAGATTTTCAAGCACGTAACAACAATGAGTTGGTGGCTGTTTACGGTAACTTTGTAAACCGTGCTTTAGTGCTG
ACTCATAAATATTTCGACGGGAAAGTTCCCGTTTGCGGTGAATTGACCGACTACGACAAAGAAACATTAAAAGAATTTGC
AGACGTAAAGGCAGAAGTAGAAAAGCTACTGGATGTATTCAAATTCCGCGATGCACAAAAAGAGGCAATGAACCTGGCAC
GTATCGGTAATAAATACTTAGCCGATACAGAGCCTTGGAAACTGGCAAAAACTGATATGGACCGTGTAGCCACCATCCTT
AACATAGCATTGCAACTAGTTGCCAATCTGGCTATTGCATTCGAACCTTTCCTGCCTTTCAGCAGCGAAAAACTCCGCAA
AATGCTGAATATGGAAACATTTGAATGGGACCAACTGGGACGTACCGATCTGCTGGCAGAAGGACATCAATTGAATAAGG
CAGAACTGTTGTTCGAAAAGATCGAAGATGAAACCATTCAAGCACAAGTAGACAAACTGTTGGCTACCAAAAAAGCAAAC
GAGGCTGCCAACTATAAAGCTAACCCGATAAAACCGGTAATTGCTTTTGAAGAATTTGAAAAGTTAGATATCCGTGTAGG
TACCGTATTAGAGTGTGAGGTAGTGCCTAAAATGAAAAAGCTGCTGAAATTCAAGATTGCTGACGGACTGGAGAACCGTA
CCATCGTCAGCGGTATCGCACAACATTATAAACCGGAAGAACTGGTGGGCAAACAAGTTTTATTCATTGCCAACCTTGCT
CCGCGCCAATTCAAGAACGGATTGGTGAGCGAAGGCATGATCCTGAGTGCTGAAAACTTTGACGGCACCTTAGCGGTGAC
CTCTTTATTAAGAGAGGTAAAGCCGGGCAGTGAAGTGAAATAA

Upstream 100 bases:

>100_bases
GAGATTTTAAGGGAGAAATATTTCTGATTATTCCATATACTTACTAAAAATCACTAACTTTGCACGTTACTGGTAGAAAT
AACAAAAAAGACATCATACA

Downstream 100 bases:

>100_bases
TATACACCCAAATACAGAAAAGGCTGAAAGCAACTGAAACGTCCGCTTTCAGCCTGACTGTTTTATAATCTATCTATAAA
AATGGCAGAACAATCCTTGA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 680; Mature: 680

Protein sequence:

>680_residues
MENKFKRTTVTSALPYANGPVHIGHLAGVYVPADIYVRYLRLKKEDVLFIGGSDEHGVPITIRAKKEGITPQDVVDRYHT
LIKESFKEFGISFDIYSRTTSKTHHELASEFFKTLYDKGEFIEKTSMQYYDEEAHQFLADRYITGECPHCHAEGAYGDQC
EKCGTSLSPTDLINPKSAISGSKPVMRETKHWYLPLDKHEGWLREWILENHKEWRPNVYGQCKSWLDMGLQPRAVSRDLD
WGIPVPVEGAEGKVLYVWFDAPIGYISNTKELLPDSWETWWKDPETRLIHFIGKDNIVFHCIVFPAMLKAEGSYILPDNV
PSNEFLNLEGDKISTSRNWAVWLHEYLQDFPGKQDVLRYVLTANAPETKDNDFTWKDFQARNNNELVAVYGNFVNRALVL
THKYFDGKVPVCGELTDYDKETLKEFADVKAEVEKLLDVFKFRDAQKEAMNLARIGNKYLADTEPWKLAKTDMDRVATIL
NIALQLVANLAIAFEPFLPFSSEKLRKMLNMETFEWDQLGRTDLLAEGHQLNKAELLFEKIEDETIQAQVDKLLATKKAN
EAANYKANPIKPVIAFEEFEKLDIRVGTVLECEVVPKMKKLLKFKIADGLENRTIVSGIAQHYKPEELVGKQVLFIANLA
PRQFKNGLVSEGMILSAENFDGTLAVTSLLREVKPGSEVK

Sequences:

>Translated_680_residues
MENKFKRTTVTSALPYANGPVHIGHLAGVYVPADIYVRYLRLKKEDVLFIGGSDEHGVPITIRAKKEGITPQDVVDRYHT
LIKESFKEFGISFDIYSRTTSKTHHELASEFFKTLYDKGEFIEKTSMQYYDEEAHQFLADRYITGECPHCHAEGAYGDQC
EKCGTSLSPTDLINPKSAISGSKPVMRETKHWYLPLDKHEGWLREWILENHKEWRPNVYGQCKSWLDMGLQPRAVSRDLD
WGIPVPVEGAEGKVLYVWFDAPIGYISNTKELLPDSWETWWKDPETRLIHFIGKDNIVFHCIVFPAMLKAEGSYILPDNV
PSNEFLNLEGDKISTSRNWAVWLHEYLQDFPGKQDVLRYVLTANAPETKDNDFTWKDFQARNNNELVAVYGNFVNRALVL
THKYFDGKVPVCGELTDYDKETLKEFADVKAEVEKLLDVFKFRDAQKEAMNLARIGNKYLADTEPWKLAKTDMDRVATIL
NIALQLVANLAIAFEPFLPFSSEKLRKMLNMETFEWDQLGRTDLLAEGHQLNKAELLFEKIEDETIQAQVDKLLATKKAN
EAANYKANPIKPVIAFEEFEKLDIRVGTVLECEVVPKMKKLLKFKIADGLENRTIVSGIAQHYKPEELVGKQVLFIANLA
PRQFKNGLVSEGMILSAENFDGTLAVTSLLREVKPGSEVK
>Mature_680_residues
MENKFKRTTVTSALPYANGPVHIGHLAGVYVPADIYVRYLRLKKEDVLFIGGSDEHGVPITIRAKKEGITPQDVVDRYHT
LIKESFKEFGISFDIYSRTTSKTHHELASEFFKTLYDKGEFIEKTSMQYYDEEAHQFLADRYITGECPHCHAEGAYGDQC
EKCGTSLSPTDLINPKSAISGSKPVMRETKHWYLPLDKHEGWLREWILENHKEWRPNVYGQCKSWLDMGLQPRAVSRDLD
WGIPVPVEGAEGKVLYVWFDAPIGYISNTKELLPDSWETWWKDPETRLIHFIGKDNIVFHCIVFPAMLKAEGSYILPDNV
PSNEFLNLEGDKISTSRNWAVWLHEYLQDFPGKQDVLRYVLTANAPETKDNDFTWKDFQARNNNELVAVYGNFVNRALVL
THKYFDGKVPVCGELTDYDKETLKEFADVKAEVEKLLDVFKFRDAQKEAMNLARIGNKYLADTEPWKLAKTDMDRVATIL
NIALQLVANLAIAFEPFLPFSSEKLRKMLNMETFEWDQLGRTDLLAEGHQLNKAELLFEKIEDETIQAQVDKLLATKKAN
EAANYKANPIKPVIAFEEFEKLDIRVGTVLECEVVPKMKKLLKFKIADGLENRTIVSGIAQHYKPEELVGKQVLFIANLA
PRQFKNGLVSEGMILSAENFDGTLAVTSLLREVKPGSEVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=552, Percent_Identity=37.5, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI24308436, Length=534, Percent_Identity=25.6554307116105, Blast_Score=138, Evalue=2e-32,
Organism=Escherichia coli, GI1788432, Length=696, Percent_Identity=34.6264367816092, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17540794, Length=564, Percent_Identity=35.1063829787234, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI212645096, Length=513, Percent_Identity=25.3411306042885, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71992781, Length=269, Percent_Identity=26.0223048327138, Blast_Score=76, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6321704, Length=548, Percent_Identity=36.8613138686131, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24655311, Length=616, Percent_Identity=35.0649350649351, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI21358483, Length=579, Percent_Identity=24.6977547495682, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_BACV8 (A6KYR3)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298199.1
- ProteinModelPortal:   A6KYR3
- SMR:   A6KYR3
- STRING:   A6KYR3
- GeneID:   5301849
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_0882
- NMPDR:   fig|435590.6.peg.840
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 77704; Mature: 77704

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKFKRTTVTSALPYANGPVHIGHLAGVYVPADIYVRYLRLKKEDVLFIGGSDEHGVPI
CCCCCCHHHHHHCCCCCCCCEEEEECCCEEECHHHHHHHHHCCCCCEEEECCCCCCCCCE
TIRAKKEGITPQDVVDRYHTLIKESFKEFGISFDIYSRTTSKTHHELASEFFKTLYDKGE
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCH
FIEKTSMQYYDEEAHQFLADRYITGECPHCHAEGAYGDQCEKCGTSLSPTDLINPKSAIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHCCCCHHHCC
GSKPVMRETKHWYLPLDKHEGWLREWILENHKEWRPNVYGQCKSWLDMGLQPRAVSRDLD
CCCCHHHCCCCEEEECCCCCHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCCCHHCCCCC
WGIPVPVEGAEGKVLYVWFDAPIGYISNTKELLPDSWETWWKDPETRLIHFIGKDNIVFH
CCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCHHHHCCCCCCEEEEEECCCCEEEE
CIVFPAMLKAEGSYILPDNVPSNEFLNLEGDKISTSRNWAVWLHEYLQDFPGKQDVLRYV
HHHHHHHHHCCCCEECCCCCCCCCEEECCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHH
LTANAPETKDNDFTWKDFQARNNNELVAVYGNFVNRALVLTHKYFDGKVPVCGELTDYDK
HHCCCCCCCCCCCCCCHHCCCCCCCEEEEECCHHCCEEEEEEEECCCCCCCCCCCCCCCH
ETLKEFADVKAEVEKLLDVFKFRDAQKEAMNLARIGNKYLADTEPWKLAKTDMDRVATIL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHH
NIALQLVANLAIAFEPFLPFSSEKLRKMLNMETFEWDQLGRTDLLAEGHQLNKAELLFEK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHCCCHHHHHCCCCCCHHHHHHHH
IEDETIQAQVDKLLATKKANEAANYKANPIKPVIAFEEFEKLDIRVGTVLECEVVPKMKK
HCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHEEEECCEEEEHHHHHHHH
LLKFKIADGLENRTIVSGIAQHYKPEELVGKQVLFIANLAPRQFKNGLVSEGMILSAENF
HHHHHHHCCCCCHHHHHHHHHHCCCHHHHCCEEEEEECCCHHHHHCCCCCCCEEEEECCC
DGTLAVTSLLREVKPGSEVK
CCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MENKFKRTTVTSALPYANGPVHIGHLAGVYVPADIYVRYLRLKKEDVLFIGGSDEHGVPI
CCCCCCHHHHHHCCCCCCCCEEEEECCCEEECHHHHHHHHHCCCCCEEEECCCCCCCCCE
TIRAKKEGITPQDVVDRYHTLIKESFKEFGISFDIYSRTTSKTHHELASEFFKTLYDKGE
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCH
FIEKTSMQYYDEEAHQFLADRYITGECPHCHAEGAYGDQCEKCGTSLSPTDLINPKSAIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHCCCCHHHCC
GSKPVMRETKHWYLPLDKHEGWLREWILENHKEWRPNVYGQCKSWLDMGLQPRAVSRDLD
CCCCHHHCCCCEEEECCCCCHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCCCHHCCCCC
WGIPVPVEGAEGKVLYVWFDAPIGYISNTKELLPDSWETWWKDPETRLIHFIGKDNIVFH
CCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCHHHHCCCCCCEEEEEECCCCEEEE
CIVFPAMLKAEGSYILPDNVPSNEFLNLEGDKISTSRNWAVWLHEYLQDFPGKQDVLRYV
HHHHHHHHHCCCCEECCCCCCCCCEEECCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHH
LTANAPETKDNDFTWKDFQARNNNELVAVYGNFVNRALVLTHKYFDGKVPVCGELTDYDK
HHCCCCCCCCCCCCCCHHCCCCCCCEEEEECCHHCCEEEEEEEECCCCCCCCCCCCCCCH
ETLKEFADVKAEVEKLLDVFKFRDAQKEAMNLARIGNKYLADTEPWKLAKTDMDRVATIL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHH
NIALQLVANLAIAFEPFLPFSSEKLRKMLNMETFEWDQLGRTDLLAEGHQLNKAELLFEK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHCCCHHHHHCCCCCCHHHHHHHH
IEDETIQAQVDKLLATKKANEAANYKANPIKPVIAFEEFEKLDIRVGTVLECEVVPKMKK
HCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHEEEECCEEEEHHHHHHHH
LLKFKIADGLENRTIVSGIAQHYKPEELVGKQVLFIANLAPRQFKNGLVSEGMILSAENF
HHHHHHHCCCCCHHHHHHHHHHCCCHHHHCCEEEEEECCCHHHHHCCCCCCCEEEEECCC
DGTLAVTSLLREVKPGSEVK
CCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA