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The map label for this gene is btuB [H]

Identifier: 150003426

GI number: 150003426

Start: 1155429

End: 1157816

Strand: Reverse

Name: btuB [H]

Synonym: BVU_0851

Alternate gene names: 150003426

Gene position: 1157816-1155429 (Counterclockwise)

Preceding gene: 150003430

Following gene: 150003425

Centisome position: 22.42

GC content: 38.86

Gene sequence:

>2388_bases
ATGGTAAAAAGACAGTTACAAAGGATCAGTTTAGGCTCTCTTTTTTTTATAATCGGAGCGATATCTATCTTTGCACAACC
TAAGACAATGGTATCCGGTAAAGTGATATCCAAAGAAAAACAGGAAGTCGTAGATTTTGCGACCGTATATTTGAAGGGTA
CAACTTATGGCTGTACAACGAATGAAGAAGGTATCTATCATCTTCATGCACCGGCAGGGAAATACACGCTGGTTGTTTCG
GCTATAGGATATAAGACTATAGAAAAGCCCGTTACTTTGGTTCATGGAGAAAGGATTAAGATGAATGTAATGATTGCCCC
GTCAGTGACTGAGTTGGGTGAAGTGGTAGTGGTGTCTAACGGGGTTTCCCGTGTGAAACGTTCCGCATTCAATGCGATAG
CTGTAGATACAAAAGAATTCCAGAATTCTACCAAGAATCTGAGTGATGCTTTGGCTAAAGCTCCGGGAATGAAACTTCGT
GAGTCGGGGGGGGTAGGTTCGGATATGCAATTGATGTTGGATGGTTTCAGTGGCAAACATGTGAAGATATTTATTGACGG
TGTACCTCAGGAGGGAGTAGGGAGCTCTTTTGGCTTGAATAATATTCCTGTGAATTTTGCAGATAGGATTGAAGTGTATA
AAGGGGTGGTTCCTGTAGGATTTGGCACGGATGCTATAGGTGGTGTTATCAATATTGTAACCAATAAGAAGAAACGTAAT
TGGTTTTTGGATGGCTCCTATTCATACGGGTCGTTCAATACGCATAAATCGTATGTGAATTTCGGACAAACTTTCAAAAA
TGGCTTTACTTATGAAATAAATGCTTTCCAAAACTATTCGGATAATGATTATCAGGTGGACGCACCGGTAGAGGACTTTG
AAACCGGAAGAATTGATAAAGATAAAAGGGTACGTGTGAAGCGCTTTAATGATACCTATCATAATGAGGCGGTTATAGGT
AAGATGGGTATTGTGGATAAGAAATGGGCAGACAGATTGATGTTGGGTTTTACTTATTCACACATGTATAAGGAGATACA
GACCGGAGTACGTCAGGAAATAGTCTATGGGGAAAAGCATCGTAAAGGACATTCGTTGATGCCGTCATTGGAATATAGTA
AGCGTGGCTTATTCATCAAAGGTCTGGATGTGGCATTGACTGCCAATTATAATAAAAATGCAACGACAAATATAGATACA
GCTTCCTATAAGTACAATTGGTTGGGTGATAGAAAATTAATGAACTCACCCGGTGAACAATCCAATCAATATTCACGGGC
GGATAATAACAATTGGAATGGAACATTAACCGTTAATTACCGTTTGGCGAAAATACATATGCTGACCTTTAATCATGTGT
TGAATACTTTCCGCCGTTCTAACACTTCTTTGTTGGCTAAGATGGAATCGGAGGATGCCATAGCTAAAGAAACCCATAAG
AATATCTCCGGATTGTCCTATCGGTTGATGCCGTCTGATAATTGGAATCTCTCAGTATTTGGAAAATATTATAGTTTGTA
TGTGGCAGGTCCTATGGCTACGACTACGAATCAAGATGATTATGTCCGTACTACCCGAAACATGAATTCCATAGGATATG
GAGCTGCGGGAACTTATTTCATTCTACCCGGTTTGCAGGCGAAATTGTCATACGAAAAAGCATATCGTCTGCCTACTATT
GAAGAAATGTTTGGAGATGAGGATTTGGAAATGGGAGATATCAGTATAAAACCGGAGAGTAGTGATAATTTGAATTTTAA
TTTGAGCTATAACAGAACATTTGGCAGGCATTCTGTATATATGGAAGGTGGAGTGATTTATCGGAATACAAAAGATTATA
TTCAGCGTAATATTGCGGATTTGAGTGGTGGTAAATATGCCGCGAAATATATTAATTACGGTAAAGTATTGACGAAAGGA
TATACTGTTTCTGCTCGTTACGGTTTTGGTAATTGGGTGAGTATAGGAGGTAATTTTACCAAAATGGATGTACGGGACAA
TATGAAAACATCTATTTCCAGCAGTGCGGAAAATCTGGCTTATAAAGAACGGATGCCGAATCTGCCCTATATGTTTGCTG
ATTCGGATGTTACTTTTTATTGGCGGGATTTAGGAAGGAAAGGCAATATGTTGACCGTGTCTTATGATAATCAATATCTG
CATAGCTTTACTTATTATTCTTCCCGAATAGGCTCAAATAAAGGGGACTATGTGGTACCTGATCAATTCTCACACAATAT
ATCCTTTTCTTATAGTTTGCAAAAAGGACGATATAACGTATCATTGGAATGTCGTAACTTCACAGATGAGAAGTTATATG
ATAATTTTAGTCTTCAGAAGGCCGGTAGGGCTTTTTATGGTAAACTGCGTGTTTGTTTCGGTAATTAA

Upstream 100 bases:

>100_bases
AATGGGGGAAAAGATTACCTATATCAGGTGATTGTTTCCCCTTTTCTTTTTACGGACCTTTGCATTCTCAAAAATTAAGA
ATTTAGAATTATAGGTATTC

Downstream 100 bases:

>100_bases
AGAATTGGAAAAAGGAGTTTATAATTAATTGGATTTAAAATTAAATAGAAGAATAAAATGAAGAAAGATTTCTTTTTGAA
CGGATTTGCTGCTATTGCTA

Product: putative TonB-linked outer membrane receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MVKRQLQRISLGSLFFIIGAISIFAQPKTMVSGKVISKEKQEVVDFATVYLKGTTYGCTTNEEGIYHLHAPAGKYTLVVS
AIGYKTIEKPVTLVHGERIKMNVMIAPSVTELGEVVVVSNGVSRVKRSAFNAIAVDTKEFQNSTKNLSDALAKAPGMKLR
ESGGVGSDMQLMLDGFSGKHVKIFIDGVPQEGVGSSFGLNNIPVNFADRIEVYKGVVPVGFGTDAIGGVINIVTNKKKRN
WFLDGSYSYGSFNTHKSYVNFGQTFKNGFTYEINAFQNYSDNDYQVDAPVEDFETGRIDKDKRVRVKRFNDTYHNEAVIG
KMGIVDKKWADRLMLGFTYSHMYKEIQTGVRQEIVYGEKHRKGHSLMPSLEYSKRGLFIKGLDVALTANYNKNATTNIDT
ASYKYNWLGDRKLMNSPGEQSNQYSRADNNNWNGTLTVNYRLAKIHMLTFNHVLNTFRRSNTSLLAKMESEDAIAKETHK
NISGLSYRLMPSDNWNLSVFGKYYSLYVAGPMATTTNQDDYVRTTRNMNSIGYGAAGTYFILPGLQAKLSYEKAYRLPTI
EEMFGDEDLEMGDISIKPESSDNLNFNLSYNRTFGRHSVYMEGGVIYRNTKDYIQRNIADLSGGKYAAKYINYGKVLTKG
YTVSARYGFGNWVSIGGNFTKMDVRDNMKTSISSSAENLAYKERMPNLPYMFADSDVTFYWRDLGRKGNMLTVSYDNQYL
HSFTYYSSRIGSNKGDYVVPDQFSHNISFSYSLQKGRYNVSLECRNFTDEKLYDNFSLQKAGRAFYGKLRVCFGN

Sequences:

>Translated_795_residues
MVKRQLQRISLGSLFFIIGAISIFAQPKTMVSGKVISKEKQEVVDFATVYLKGTTYGCTTNEEGIYHLHAPAGKYTLVVS
AIGYKTIEKPVTLVHGERIKMNVMIAPSVTELGEVVVVSNGVSRVKRSAFNAIAVDTKEFQNSTKNLSDALAKAPGMKLR
ESGGVGSDMQLMLDGFSGKHVKIFIDGVPQEGVGSSFGLNNIPVNFADRIEVYKGVVPVGFGTDAIGGVINIVTNKKKRN
WFLDGSYSYGSFNTHKSYVNFGQTFKNGFTYEINAFQNYSDNDYQVDAPVEDFETGRIDKDKRVRVKRFNDTYHNEAVIG
KMGIVDKKWADRLMLGFTYSHMYKEIQTGVRQEIVYGEKHRKGHSLMPSLEYSKRGLFIKGLDVALTANYNKNATTNIDT
ASYKYNWLGDRKLMNSPGEQSNQYSRADNNNWNGTLTVNYRLAKIHMLTFNHVLNTFRRSNTSLLAKMESEDAIAKETHK
NISGLSYRLMPSDNWNLSVFGKYYSLYVAGPMATTTNQDDYVRTTRNMNSIGYGAAGTYFILPGLQAKLSYEKAYRLPTI
EEMFGDEDLEMGDISIKPESSDNLNFNLSYNRTFGRHSVYMEGGVIYRNTKDYIQRNIADLSGGKYAAKYINYGKVLTKG
YTVSARYGFGNWVSIGGNFTKMDVRDNMKTSISSSAENLAYKERMPNLPYMFADSDVTFYWRDLGRKGNMLTVSYDNQYL
HSFTYYSSRIGSNKGDYVVPDQFSHNISFSYSLQKGRYNVSLECRNFTDEKLYDNFSLQKAGRAFYGKLRVCFGN
>Mature_795_residues
MVKRQLQRISLGSLFFIIGAISIFAQPKTMVSGKVISKEKQEVVDFATVYLKGTTYGCTTNEEGIYHLHAPAGKYTLVVS
AIGYKTIEKPVTLVHGERIKMNVMIAPSVTELGEVVVVSNGVSRVKRSAFNAIAVDTKEFQNSTKNLSDALAKAPGMKLR
ESGGVGSDMQLMLDGFSGKHVKIFIDGVPQEGVGSSFGLNNIPVNFADRIEVYKGVVPVGFGTDAIGGVINIVTNKKKRN
WFLDGSYSYGSFNTHKSYVNFGQTFKNGFTYEINAFQNYSDNDYQVDAPVEDFETGRIDKDKRVRVKRFNDTYHNEAVIG
KMGIVDKKWADRLMLGFTYSHMYKEIQTGVRQEIVYGEKHRKGHSLMPSLEYSKRGLFIKGLDVALTANYNKNATTNIDT
ASYKYNWLGDRKLMNSPGEQSNQYSRADNNNWNGTLTVNYRLAKIHMLTFNHVLNTFRRSNTSLLAKMESEDAIAKETHK
NISGLSYRLMPSDNWNLSVFGKYYSLYVAGPMATTTNQDDYVRTTRNMNSIGYGAAGTYFILPGLQAKLSYEKAYRLPTI
EEMFGDEDLEMGDISIKPESSDNLNFNLSYNRTFGRHSVYMEGGVIYRNTKDYIQRNIADLSGGKYAAKYINYGKVLTKG
YTVSARYGFGNWVSIGGNFTKMDVRDNMKTSISSSAENLAYKERMPNLPYMFADSDVTFYWRDLGRKGNMLTVSYDNQYL
HSFTYYSSRIGSNKGDYVVPDQFSHNISFSYSLQKGRYNVSLECRNFTDEKLYDNFSLQKAGRAFYGKLRVCFGN

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=154, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=7e-13,
Organism=Escherichia coli, GI1788478, Length=142, Percent_Identity=31.6901408450704, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 89686; Mature: 89686

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKRQLQRISLGSLFFIIGAISIFAQPKTMVSGKVISKEKQEVVDFATVYLKGTTYGCTT
CCHHHHHHHCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHEEEEEEEECCEECCCC
NEEGIYHLHAPAGKYTLVVSAIGYKTIEKPVTLVHGERIKMNVMIAPSVTELGEVVVVSN
CCCCEEEEECCCCCEEEEEEECCCHHHCCCEEEEECCEEEEEEEECCCHHHCCCEEEEEC
GVSRVKRSAFNAIAVDTKEFQNSTKNLSDALAKAPGMKLRESGGVGSDMQLMLDGFSGKH
CHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECCCCCE
VKIFIDGVPQEGVGSSFGLNNIPVNFADRIEVYKGVVPVGFGTDAIGGVINIVTNKKKRN
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEECCCCHHHCCEEEEEECCCCCC
WFLDGSYSYGSFNTHKSYVNFGQTFKNGFTYEINAFQNYSDNDYQVDAPVEDFETGRIDK
EEECCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCEEECCCHHHCCCCCCCC
DKRVRVKRFNDTYHNEAVIGKMGIVDKKWADRLMLGFTYSHMYKEIQTGVRQEIVYGEKH
CCCEEEEECCCCCCCCEEEEECCCCCHHHHHHEEEEHHHHHHHHHHHHHHHHHHHCCCCC
RKGHSLMPSLEYSKRGLFIKGLDVALTANYNKNATTNIDTASYKYNWLGDRKLMNSPGEQ
CCCCCCCCCCCCCCCCEEEEECEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHCCCCCC
SNQYSRADNNNWNGTLTVNYRLAKIHMLTFNHVLNTFRRSNTSLLAKMESEDAIAKETHK
CCCCCCCCCCCCCEEEEEEEEEEEEEEEHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHC
NISGLSYRLMPSDNWNLSVFGKYYSLYVAGPMATTTNQDDYVRTTRNMNSIGYGAAGTYF
CCCCCEEEEECCCCCCEEEEEEEEEEEEECCCCCCCCCCCHHHECCCCCCCCCCCCCCEE
ILPGLQAKLSYEKAYRLPTIEEMFGDEDLEMGDISIKPESSDNLNFNLSYNRTFGRHSVY
EECCCCEEECHHHHHCCCCHHHHCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCEEEE
MEGGVIYRNTKDYIQRNIADLSGGKYAAKYINYGKVLTKGYTVSARYGFGNWVSIGGNFT
EECCEEEECCHHHHHHHHHHCCCCCEEEHHHCCCCEEECCEEEEEECCCCCEEEECCCEE
KMDVRDNMKTSISSSAENLAYKERMPNLPYMFADSDVTFYWRDLGRKGNMLTVSYDNQYL
EEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCEEEEEECCCCH
HSFTYYSSRIGSNKGDYVVPDQFSHNISFSYSLQKGRYNVSLECRNFTDEKLYDNFSLQK
HHHHHHHHHCCCCCCCEECCCCCCCCEEEEEEEECCEEEEEEEECCCCCHHHHCCCCHHH
AGRAFYGKLRVCFGN
HCCEEEEEEEEEECC
>Mature Secondary Structure
MVKRQLQRISLGSLFFIIGAISIFAQPKTMVSGKVISKEKQEVVDFATVYLKGTTYGCTT
CCHHHHHHHCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHEEEEEEEECCEECCCC
NEEGIYHLHAPAGKYTLVVSAIGYKTIEKPVTLVHGERIKMNVMIAPSVTELGEVVVVSN
CCCCEEEEECCCCCEEEEEEECCCHHHCCCEEEEECCEEEEEEEECCCHHHCCCEEEEEC
GVSRVKRSAFNAIAVDTKEFQNSTKNLSDALAKAPGMKLRESGGVGSDMQLMLDGFSGKH
CHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECCCCCE
VKIFIDGVPQEGVGSSFGLNNIPVNFADRIEVYKGVVPVGFGTDAIGGVINIVTNKKKRN
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEECCCCHHHCCEEEEEECCCCCC
WFLDGSYSYGSFNTHKSYVNFGQTFKNGFTYEINAFQNYSDNDYQVDAPVEDFETGRIDK
EEECCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCEEECCCHHHCCCCCCCC
DKRVRVKRFNDTYHNEAVIGKMGIVDKKWADRLMLGFTYSHMYKEIQTGVRQEIVYGEKH
CCCEEEEECCCCCCCCEEEEECCCCCHHHHHHEEEEHHHHHHHHHHHHHHHHHHHCCCCC
RKGHSLMPSLEYSKRGLFIKGLDVALTANYNKNATTNIDTASYKYNWLGDRKLMNSPGEQ
CCCCCCCCCCCCCCCCEEEEECEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHCCCCCC
SNQYSRADNNNWNGTLTVNYRLAKIHMLTFNHVLNTFRRSNTSLLAKMESEDAIAKETHK
CCCCCCCCCCCCCEEEEEEEEEEEEEEEHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHC
NISGLSYRLMPSDNWNLSVFGKYYSLYVAGPMATTTNQDDYVRTTRNMNSIGYGAAGTYF
CCCCCEEEEECCCCCCEEEEEEEEEEEEECCCCCCCCCCCHHHECCCCCCCCCCCCCCEE
ILPGLQAKLSYEKAYRLPTIEEMFGDEDLEMGDISIKPESSDNLNFNLSYNRTFGRHSVY
EECCCCEEECHHHHHCCCCHHHHCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCEEEE
MEGGVIYRNTKDYIQRNIADLSGGKYAAKYINYGKVLTKGYTVSARYGFGNWVSIGGNFT
EECCEEEECCHHHHHHHHHHCCCCCEEEHHHCCCCEEECCEEEEEECCCCCEEEECCCEE
KMDVRDNMKTSISSSAENLAYKERMPNLPYMFADSDVTFYWRDLGRKGNMLTVSYDNQYL
EEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCEEEEEECCCCH
HSFTYYSSRIGSNKGDYVVPDQFSHNISFSYSLQKGRYNVSLECRNFTDEKLYDNFSLQK
HHHHHHHHHCCCCCCCEECCCCCCCCEEEEEEEECCEEEEEEEECCCCCHHHHCCCCHHH
AGRAFYGKLRVCFGN
HCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA