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The map label for this gene is ahpF [H]

Identifier: 150003421

GI number: 150003421

Start: 1149105

End: 1150655

Strand: Reverse

Name: ahpF [H]

Synonym: BVU_0846

Alternate gene names: 150003421

Gene position: 1150655-1149105 (Counterclockwise)

Preceding gene: 150003422

Following gene: 150003420

Centisome position: 22.29

GC content: 44.17

Gene sequence:

>1551_bases
ATGTTAGATAGTGCGATAAAAGATCAGTTAAAAGGTCTTTTTGCCCAATTGGAAGCGCACTATACTTTTGACATCTTTGT
CCACCCTCAGCACGAAAGTCGTGCCGAGTTGGTGGACTTGTTGGAAGAAGTGGCTTCCTGTTCGGATAAACTTTCCTGTC
GGTTGCAGGATAGTGAAGGGCTGAAGTTTATCCTTTTAAAAGAAGGAAAGGATACGGGAATCACATTTCGTGCCGTACCT
GGTGGTCATGAATTTACTTCTTTGTTGATGGCTGTTCTGAATGCTGACGGCAAAGGAAAGAATTTTCCGGACGAGTTTAT
TACCAGACGTATCAAGGCATTACAGGGGGCGATTAGTTTGACCACCTATTTGTCTCTCACCTGTACCAATTGTCCCGATG
TGGTTCAAGCTTTGAATATGATGGTACTTCTGAATGGTCAGATACGCCATGAGGCAGTAGATGGATCTATAAATGAAGAA
GAAGTAGAACGGATGAAAGTACAGGCCGTTCCTACTGTATTTGCTGATGGTGAACAGATTCATGTGGGGCGTAGCAGTAT
GGGGGATCTGTTGGAGAAGCTGGAAGCCCGTTACGGTTCGGTGGAATTGGAAGCTGTTGAAACAAAAGAATATGATGTAC
TGGTAGCCGGTGCTGGTCCTGCCGGTGCGACAGCTGCTATCTACTCGGCTCGTAAAGGGTTGAAAGTAGCAATAATAGCC
GAGCGTATTGGTGGACAAGTCAATGAAACGATGGGGATTGAGAACTTGATATCTGTGCATCAGACTACCGGTAAACAGTT
GGCCCAGGATCTGAAAAAACATTTGGCGGAATATCATATTGATATTTTAGAAAATCGCCGGATAGAGAAAGTGGAAGTGG
CAGAGGGCATGAAAGTGTTGTCTGTGAAAGGTGGAGAAACGTATAAGGCTCCCGTACTGATCATTGCTACCGGGGCGAAT
TGGAGAAAATTGAATGTTCCCGGTGAAGAAAAATACATCGGTCATGGAGTTGCTTTCTGTCCTCATTGTGACGGACCATT
TTATAAAAATAAGGAAGTAGCAGTCATAGGTGGTGGAAACTCCGGGGTTGAGGCTGCTATTGATTTGGCGGGAGTCTGTT
CCAAAGTGACTGTATTCGAATTCATGGATACTTTGAAAGCGGATACTGTTTTGCAGGAGAAAGCTAAAAGCCTGCCCAAT
GTGGATATTATTACTAATACTCAAACGGTAGAGGTGTTAGGAAACGGTGATAAAGTGGTGGGGCTTATAAAGAAAGACCG
TTCATCCGAAAAAGAAGAGATCTTTGCCTTGGATGGTATTTTTGTCCAAATAGGTTTGACTGCCAATAGCACTTTGTTTA
AAGAATTGCTGGAAACGAACCGCATGGGTGAAATTCTGACTGATAAAAATGGCCGGACCTCTGTAAAAGGTATCTATGCT
GCCGGAGATGTAACGGATGTTTCCTATAAACAGATAGTTATAGCAATGGGGGAAGGCGCCAAGGCTGCTTTAGCTGCATT
CGAAGACCGGATGCGCGGAACGATTTATTAG

Upstream 100 bases:

>100_bases
CACTCATGATGGTGAGGTTTGTCCTGCTAAATGGAAGAAAGGTGCTGAGACCTTGAAACCGAGTATTGATTTGGTAGGTA
AAATTTAAAGTATAGAACTC

Downstream 100 bases:

>100_bases
TGATAAGTGGTTGGTGGTGAATAGTGGGTGCATATCGCAGTATTTACCACTAATCTTATCACTAAAAAATGCACTCTTTG
TATGTATATTGGTGAGAATA

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MLDSAIKDQLKGLFAQLEAHYTFDIFVHPQHESRAELVDLLEEVASCSDKLSCRLQDSEGLKFILLKEGKDTGITFRAVP
GGHEFTSLLMAVLNADGKGKNFPDEFITRRIKALQGAISLTTYLSLTCTNCPDVVQALNMMVLLNGQIRHEAVDGSINEE
EVERMKVQAVPTVFADGEQIHVGRSSMGDLLEKLEARYGSVELEAVETKEYDVLVAGAGPAGATAAIYSARKGLKVAIIA
ERIGGQVNETMGIENLISVHQTTGKQLAQDLKKHLAEYHIDILENRRIEKVEVAEGMKVLSVKGGETYKAPVLIIATGAN
WRKLNVPGEEKYIGHGVAFCPHCDGPFYKNKEVAVIGGGNSGVEAAIDLAGVCSKVTVFEFMDTLKADTVLQEKAKSLPN
VDIITNTQTVEVLGNGDKVVGLIKKDRSSEKEEIFALDGIFVQIGLTANSTLFKELLETNRMGEILTDKNGRTSVKGIYA
AGDVTDVSYKQIVIAMGEGAKAALAAFEDRMRGTIY

Sequences:

>Translated_516_residues
MLDSAIKDQLKGLFAQLEAHYTFDIFVHPQHESRAELVDLLEEVASCSDKLSCRLQDSEGLKFILLKEGKDTGITFRAVP
GGHEFTSLLMAVLNADGKGKNFPDEFITRRIKALQGAISLTTYLSLTCTNCPDVVQALNMMVLLNGQIRHEAVDGSINEE
EVERMKVQAVPTVFADGEQIHVGRSSMGDLLEKLEARYGSVELEAVETKEYDVLVAGAGPAGATAAIYSARKGLKVAIIA
ERIGGQVNETMGIENLISVHQTTGKQLAQDLKKHLAEYHIDILENRRIEKVEVAEGMKVLSVKGGETYKAPVLIIATGAN
WRKLNVPGEEKYIGHGVAFCPHCDGPFYKNKEVAVIGGGNSGVEAAIDLAGVCSKVTVFEFMDTLKADTVLQEKAKSLPN
VDIITNTQTVEVLGNGDKVVGLIKKDRSSEKEEIFALDGIFVQIGLTANSTLFKELLETNRMGEILTDKNGRTSVKGIYA
AGDVTDVSYKQIVIAMGEGAKAALAAFEDRMRGTIY
>Mature_516_residues
MLDSAIKDQLKGLFAQLEAHYTFDIFVHPQHESRAELVDLLEEVASCSDKLSCRLQDSEGLKFILLKEGKDTGITFRAVP
GGHEFTSLLMAVLNADGKGKNFPDEFITRRIKALQGAISLTTYLSLTCTNCPDVVQALNMMVLLNGQIRHEAVDGSINEE
EVERMKVQAVPTVFADGEQIHVGRSSMGDLLEKLEARYGSVELEAVETKEYDVLVAGAGPAGATAAIYSARKGLKVAIIA
ERIGGQVNETMGIENLISVHQTTGKQLAQDLKKHLAEYHIDILENRRIEKVEVAEGMKVLSVKGGETYKAPVLIIATGAN
WRKLNVPGEEKYIGHGVAFCPHCDGPFYKNKEVAVIGGGNSGVEAAIDLAGVCSKVTVFEFMDTLKADTVLQEKAKSLPN
VDIITNTQTVEVLGNGDKVVGLIKKDRSSEKEEIFALDGIFVQIGLTANSTLFKELLETNRMGEILTDKNGRTSVKGIYA
AGDVTDVSYKQIVIAMGEGAKAALAAFEDRMRGTIY

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=519, Percent_Identity=45.6647398843931, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1787114, Length=309, Percent_Identity=37.2168284789644, Blast_Score=165, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6321898, Length=310, Percent_Identity=33.8709677419355, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320560, Length=308, Percent_Identity=34.0909090909091, Blast_Score=148, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 55995; Mature: 55995

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSAIKDQLKGLFAQLEAHYTFDIFVHPQHESRAELVDLLEEVASCSDKLSCRLQDSEG
CCCHHHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCC
LKFILLKEGKDTGITFRAVPGGHEFTSLLMAVLNADGKGKNFPDEFITRRIKALQGAISL
CEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEE
TTYLSLTCTNCPDVVQALNMMVLLNGQIRHEAVDGSINEEEVERMKVQAVPTVFADGEQI
EEEEEEEECCCHHHHHHHCEEEEECCCEEEHHCCCCCCHHHHHHHHHHCCCEEECCCCEE
HVGRSSMGDLLEKLEARYGSVELEAVETKEYDVLVAGAGPAGATAAIYSARKGLKVAIIA
EECCHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEE
ERIGGQVNETMGIENLISVHQTTGKQLAQDLKKHLAEYHIDILENRRIEKVEVAEGMKVL
HHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHCCCEEE
SVKGGETYKAPVLIIATGANWRKLNVPGEEKYIGHGVAFCPHCDGPFYKNKEVAVIGGGN
EECCCCCEECCEEEEEECCCEEEECCCCCCCEECCCEEECCCCCCCCCCCCEEEEEECCC
SGVEAAIDLAGVCSKVTVFEFMDTLKADTVLQEKAKSLPNVDIITNTQTVEVLGNGDKVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEECCCEEE
GLIKKDRSSEKEEIFALDGIFVQIGLTANSTLFKELLETNRMGEILTDKNGRTSVKGIYA
EEEECCCCCCHHHEEEEEEEEEEEEECCCHHHHHHHHHCCCCCCCEECCCCCCCEEEEEE
AGDVTDVSYKQIVIAMGEGAKAALAAFEDRMRGTIY
ECCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLDSAIKDQLKGLFAQLEAHYTFDIFVHPQHESRAELVDLLEEVASCSDKLSCRLQDSEG
CCCHHHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCC
LKFILLKEGKDTGITFRAVPGGHEFTSLLMAVLNADGKGKNFPDEFITRRIKALQGAISL
CEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEE
TTYLSLTCTNCPDVVQALNMMVLLNGQIRHEAVDGSINEEEVERMKVQAVPTVFADGEQI
EEEEEEEECCCHHHHHHHCEEEEECCCEEEHHCCCCCCHHHHHHHHHHCCCEEECCCCEE
HVGRSSMGDLLEKLEARYGSVELEAVETKEYDVLVAGAGPAGATAAIYSARKGLKVAIIA
EECCHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEE
ERIGGQVNETMGIENLISVHQTTGKQLAQDLKKHLAEYHIDILENRRIEKVEVAEGMKVL
HHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHCCCEEE
SVKGGETYKAPVLIIATGANWRKLNVPGEEKYIGHGVAFCPHCDGPFYKNKEVAVIGGGN
EECCCCCEECCEEEEEECCCEEEECCCCCCCEECCCEEECCCCCCCCCCCCEEEEEECCC
SGVEAAIDLAGVCSKVTVFEFMDTLKADTVLQEKAKSLPNVDIITNTQTVEVLGNGDKVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEECCCEEE
GLIKKDRSSEKEEIFALDGIFVQIGLTANSTLFKELLETNRMGEILTDKNGRTSVKGIYA
EEEECCCCCCHHHEEEEEEEEEEEEECCCHHHHHHHHHCCCCCCCEECCCCCCCEEEEEE
AGDVTDVSYKQIVIAMGEGAKAALAAFEDRMRGTIY
ECCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]